RESUMO
To investigate the in vitro anthelmintic efficacy of dividivi (Caesalpinia coriaria), a traditional medicinal plant used in Central America and the northern part of South America, extracts from the foliage of this plant were subjected to the egg hatching test (EHT) and larval exsheathment inhibition test (LEIT), against Haemonchus contortus. Four different extracts were evaluated: acetone-water (AW), methanol-water (MW), acetone-water-dichloromethane (AWD) and methanol-water-dichloromethane (MWD). The concentrations used for the EHT and LEIT tests ranged from 500 to 4000 µg mL-1 and six repetitions per concentration. The effective concentrations (EC50) were calculated using Probit analysis. The EC50 for EHT were 2947.0, 3347.0, 3959.6 and 4538.7 µg mL-1 for MWD, MW, AW and AWD, respectively. The EC50 for LEIT were 2883.4, 5927.4, 9876.3 and 9955.4 µg mL-1 for AWD, AW, MWD and MW, respectively. The methanol extracts were the most effective in inhibiting the hatching of eggs, while the acetone extracts showed efficacy in inhibiting larval exsheathment. This study explains the importance that C. coriaria has as a medicinal plant in Central and South American countries.(AU)
Para investigar a eficácia anti-helmíntica de Divi-divi (Caesalpinia coriaria), uma planta medicinal tradicional usada na América Central e no norte da América do Sul. Extratos das folhas dessa planta foram utilizados em testes in vitro de inibição da eclosão de ovos (EHT) e desembainhamento larvar (LEIT) de Haemonchus contortus. Quatro diferentes extratos foram avaliados: acetona-água (AW), metanol-água (MW), acetona-água-diclorometano (AWD) e metanol-água-diclorometano (MWD). Para os testes EHT e LEIT, as concentrações utilizadas variaram de 500 a 4000 µg mL-1, em seis repetições por concentração. As concentrações efetivas (EC50) foram calculadas, usando-se a análise Probit. A EC50 para EHT foram 2947,0; 3347,0; 3959,6 e 4538,7 µg mL-1 para MWD, MW, AW e AWD, respectivamente. As EC50 para LEIT foram 2883,4; 5927,4; 9876,3 e 9955,4 µg mL-1 para AWD, AW, MWD e MW, respectivamente. Os extratos de metanol foram os mais eficazes em inibir a eclosão de ovos, enquanto os extratos de acetona mostraram-se eficazes em inibir a desembainhamento larvar. Este estudo ajuda a explicar a importância da C. coriaria como planta medicinal nos países da América Central e América do Sul.(AU)
Assuntos
Animais , Ruminantes/parasitologia , Antiparasitários/análise , Gastroenteropatias/parasitologia , Anti-Helmínticos/efeitos adversos , Anti-Helmínticos/análise , Fabaceae/parasitologia , Infecções por Strongylida/parasitologia , Plantas Medicinais/toxicidadeRESUMO
Abstract To investigate the in vitro anthelmintic efficacy of dividivi (Caesalpinia coriaria), a traditional medicinal plant used in Central America and the northern part of South America, extracts from the foliage of this plant were subjected to the egg hatching test (EHT) and larval exsheathment inhibition test (LEIT), against Haemonchus contortus. Four different extracts were evaluated: acetone-water (AW), methanol-water (MW), acetone-water-dichloromethane (AWD) and methanol-water-dichloromethane (MWD). The concentrations used for the EHT and LEIT tests ranged from 500 to 4000 µg mL-1 and six repetitions per concentration. The effective concentrations (EC50) were calculated using Probit analysis. The EC50 for EHT were 2947.0, 3347.0, 3959.6 and 4538.7 µg mL-1 for MWD, MW, AW and AWD, respectively. The EC50 for LEIT were 2883.4, 5927.4, 9876.3 and 9955.4 µg mL-1 for AWD, AW, MWD and MW, respectively. The methanol extracts were the most effective in inhibiting the hatching of eggs, while the acetone extracts showed efficacy in inhibiting larval exsheathment. This study explains the importance that C. coriaria has as a medicinal plant in Central and South American countries.
Resumo Para investigar a eficácia anti-helmíntica de Divi-divi (Caesalpinia coriaria), uma planta medicinal tradicional usada na América Central e no norte da América do Sul. Extratos das folhas dessa planta foram utilizados em testes in vitro de inibição da eclosão de ovos (EHT) e desembainhamento larvar (LEIT) de Haemonchus contortus. Quatro diferentes extratos foram avaliados: acetona-água (AW), metanol-água (MW), acetona-água-diclorometano (AWD) e metanol-água-diclorometano (MWD). Para os testes EHT e LEIT, as concentrações utilizadas variaram de 500 a 4000 µg mL-1, em seis repetições por concentração. As concentrações efetivas (EC50) foram calculadas, usando-se a análise Probit. A EC50 para EHT foram 2947,0; 3347,0; 3959,6 e 4538,7 µg mL-1 para MWD, MW, AW e AWD, respectivamente. As EC50 para LEIT foram 2883,4; 5927,4; 9876,3 e 9955,4 µg mL-1 para AWD, AW, MWD e MW, respectivamente. Os extratos de metanol foram os mais eficazes em inibir a eclosão de ovos, enquanto os extratos de acetona mostraram-se eficazes em inibir a desembainhamento larvar. Este estudo ajuda a explicar a importância da C. coriaria como planta medicinal nos países da América Central e América do Sul.
Assuntos
Animais , Caesalpinia , Haemonchus , Anti-Helmínticos/farmacologia , Extratos Vegetais/farmacologia , LarvaRESUMO
BACKGROUND: Leucaena leucocephala, as a shrub legume rich in condensed tannins, is a candidate for the integrated control of gastrointestinal parasitic pathogen nematodes. Here, we investigated the anthelmintic potential of the nutraceutical L. leucocephala, transformed into pellets, against Haemonchus contortus. RESULTS: Creole goat kids were fed an iso-nitrogenous diet of Dichantium hay with alfalfa pellets or Leucaena pellets or an alfalfa-leucaena mixture in varying ratios. The artificial infection of kids with H. contortus led to infection levels comparable to those observed in the farm environment on the basis of egg excretion. The anthelmintic activity of Leucaena, compared to alfalfa, was demonstrated by its potential to reduce egg excretions (1524 vs. 3651 eggs/g) and the larval development of excreted eggs (3.5 vs. 24%). This anthelmintic potential was reported when the ratio of Leucaena incorporation in the diet was high (50% DM intake). The voluntary dry matter intake (79.3 vs. 77.0 g Large Weigth0.75), the total tract dry matter digestion (0.585 vs. 0.620), and the growth (57.1 vs. 71.3 g/d) of kids fed Leucaena compared to alfalfa indicate that Leucaena has a high feed value. The Leucaena, even at the highest intakes in the diets, has not shown any signs of poisoning in kids. CONCLUSIONS: Leucaena fulfilled the conditions to be a good nutraceutical, and pelleting is a good method for its use.
Assuntos
Anti-Helmínticos/uso terapêutico , Doenças das Cabras/tratamento farmacológico , Hemoncose/veterinária , Haemonchus/efeitos dos fármacos , Ração Animal/análise , Animais , Dieta/veterinária , Suplementos Nutricionais , Feminino , Doenças das Cabras/parasitologia , Cabras , Hemoncose/tratamento farmacológico , Haemonchus/crescimento & desenvolvimento , Masculino , Medicago sativa , Contagem de Ovos de Parasitas/veterinária , Folhas de PlantaRESUMO
Nonhuman primates host a variety of gastrointestinal parasites that infect individuals through different transmission routes. Social contact among group members (e.g., body contact, grooming) brings the risk of parasite infection, especially when the pathogen infection is directly transmitted. Along with this, accidental provisioning (i.e., food provisioning occurring during close tourist-wildlife interactions) is also considered to increase the risk of infection, as aggregation during feeding can cause higher exposure to parasite infective stages. However, while some attention has been paid to the relationship between social behavior and parasites, the link between accidental food provisioning and characteristics of parasite infection in primates has thus far received less attention. This study examines the potential effect of accidental provisioning on patterns of inter-individual spatial association, and in turn on parasite infection risk in a wild group of black capuchin monkeys (Sapajus nigritus) in Iguazú National Park, Argentina. To do so, we simulated events of accidental provisioning via researcher-managed provisioning experiments and tested whether experimental provisioning affects the inter-individual spatial distribution within groups. In addition, we determined whether patterns of parasite infection were better predicted by naturally occurring spatial networks (i.e., spatial association during natural observations) or by provisioning spatial networks (i.e., spatial interactions during experimental provisioning). We found a significant increase in network centrality that was potentially associated with an overall increase in individual connections with other group members during experimental trials. However, when assessing the effects of natural and provisioning network metrics on parasite characteristics, we did not observe a significant effect of centrality measures (i.e., closeness and betweenness) on parasite richness and single infection by Filariopsis sp. Taken together, our findings suggest that alterations of within-group spatial networks due to accidental provisioning may have a limited influence in determining the characteristics of parasite infections in black capuchin monkeys.
Assuntos
Cebus/parasitologia , Alimentos , Doenças Parasitárias/transmissão , Comportamento Social , Animais , Animais Selvagens/parasitologia , Argentina , Trato Gastrointestinal/parasitologia , Asseio AnimalRESUMO
The mechanisms responsible for the imbalance between oxidants and antioxidants in sheep infected with Haemonchus contortus are not well established. This study aimed to prove the hypothesis that oxidative stress occurring during infection by H. contortus varies according to breed, and that the parasite burden correlates with hypoalbuminaemia and anaemia. Thus, after deworming and confirming the absence of infection, two different sheep breeds, Suffolk (n = 15) and Santa Ines (n = 22), were orally inoculated with a single dose of 5,000 L3 of H. contortus. The egg counts per gram of faeces (EPG), packed cell volume (PCV) and concentrations of several plasma markers of oxidative stress (lipid peroxidation, albumin, uric acid, total bilirubin, total antioxidant capacity [TAC], total oxidant concentration [TOC] and the oxidative stress index [OSI]) were quantified before (control group) and during the experimental infection (28, 34 and 42 days post-inoculation). In both breeds, TOC increased at 28 days and TAC increased at 42 days. In Suffolk sheep, there was a positive correlation of EPG with oxidant components (28 days) and a negative correlation of EPG with PCV (42 days). In Santa Ines sheep, there was a positive correlation of EPG with bilirubin (r = 0.492; p = 0.020). H. contortus infection caused oxidative stress, which varied according to the breed. Parasite burden was not associated with hypoalbuminaemia, whereas there was a negative correlation with PCV. This research provides the first evidence that the antioxidant status contributes more to the resilience to H. contortus in Santa Ines sheep compared to Suffolk sheep.
Assuntos
Anemia/veterinária , Hemoncose/veterinária , Haemonchus/fisiologia , Hipoalbuminemia/veterinária , Estresse Oxidativo , Doenças dos Ovinos/parasitologia , Anemia/parasitologia , Animais , Fezes/parasitologia , Feminino , Gastroenteropatias/parasitologia , Hemoncose/parasitologia , Hematócrito/veterinária , Hipoalbuminemia/parasitologia , Larva , Peroxidação de Lipídeos , Masculino , Contagem de Ovos de Parasitas/veterinária , OvinosRESUMO
Intensive production systems require high-yield genetics as obtained in Bos taurus × Bos indicus crossbreeding. Generally, high-producing taurine cattle are more susceptible to parasites and heat stress. This study evaluated animal performance, heat-stress measurement (infrared temperatures), and internal parasite infection with daily weight gain in heifers from 2 genetic groups (Nelore and F1-Nelore × Angus) reared in 2 forage production systems (with or without crop-livestock system) during 1 yr. The main objectives were to determine the relationship between infrared measures and animal performance and whether it differs between genetic groups and environments. Thirty-six heifers were randomly assigned to 2 forage production systems, one considered as high-input system with crop-livestock system and other exclusive livestock system considered as low input. At each 28 d, infrared thermography (IR) temperatures, weight, and internal parasite infection (fecal egg count) were measured. The temperatures of the eye, snout, forehead, dewlap, body, ground and squeeze chute were determined. F1 heifers had higher weight gain than Nelore (P < 0.05) and both did not differ in internal parasite infection (P > 0.05). F1 heifers had higher IR than Nelore (P < 0.05). The main body points that differentiate between genetic groups were dewlap, forehead, and eye. Higher dewlap IR temperature (DW) was associated with higher average daily gain (ADG) during dry season (independently of genetic groups) (ADG = -0.755 + 0.032 × DW; R2 = 0.44). Otherwise, the IR temperatures had a negative relationship with ADG during rainy season and low forehead IR temperature was related to higher average daily gain (ADG = 1.81 - 0.033 × forehead; R2 = 0.12 for F1 animals and ADG = 1.46 - 0.025 × forehead; R2 = 0.07 for Nelore). The infrared temperatures were more related to animal performance during the dry season, which had high temperature and low humidity. The infrared temperatures were able to identify the animal response to the environment challenge. Animals with higher temperatures (dewlap and forehead) had higher daily gain during the dry season.