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Objective To investigate the SENP1 and c-myb gene expression and their correlations in bone marrow specimens in the patients with acute lymphoblastic leukemia (ALL ) to provide the basis for expounding the role ,mechanism and prognosis of SENP1 and c-myb in ALL .Methods 31 patients diagnosed with ALL (22 cases of B-ALL ,1 case of T-ALL and 8 cases of uncate-gorized ALL ;6 cases in the low/medium risk group ,25 cases in the high risk group) and 31 patients with proliferative bone marrow and hyperplastic anemia diagnosed by the morphology were taken as the control group .The real-time PCR and immunocytochemical staining(SP method) were adopted to detect the mRNA and protein expressions of SENP1 and c-myb in the bone marrow specimens of the ALL patients and the control group .Results The expression of SENP1 and c-myb were both increased in the bone marrow specimens and smears of ALL patients ,which showed the statistical difference compared with the control group (P< 0 .05) ,the Pearson correlation analysis found that the high expression of SENP1 and c-myb had correlation .The expression of SENP1and c-myb in the low/medium risk group were lower than that in the high risk group ,but the difference had no statistical significance . Conclusion The high expression of SENP1 and c-myb exists in the bone marrow specimens of the ALL patients ,SENP1 and c-myb could possibly have the correlation with the occurrence and development of ALL ;but now the differences of SENP1 and c-myb ex-pression among different risk groups of ALL patients are yet to be proven .
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Objective: To study the expression of the B-myb and cyclin D1 in human hepatocellular carcinoma (HCC) tissue and explore their correlation with initiation and progression of HCC. Methods: The expressions of B-myb and cyclin D1 in 60 cases of HCC tissue and the corresponding pericancerous liver tissue were detected by immunohistochemical method. Results: The positive rates of B-myb and cyclin D1 in the HCC tissue were higher than those in the pericancerous liver tissue (56.67% vs 36.67% and 50% vs 31.67%, P < 0.05) respectively. The expression of B-myb in the HCC tissue significantly correlated with the clinical stage, the extrahepatic metastasis, post-operative recurrence, and the number of tumor nodes but not correlated with the tumor thrombus in portal vein, the tumor diameter, the serum level of alpha-fetoprotein (AFP), and tumor differentiation. The expression of cyclin D1 in the HCC tissue was significantly associated with the clinical stage, the tumor thrombus in portal vein, extrahepatic metastasis, post-operative recurrence, the number of tumor nodes, and the differentiation of tumor but not associated with the tumor diameter and the serum level of AFP. The expression of B-myb had positive correlation with expression of cyclin D1 in HCC tissues. Conclusion: The overexpressions of B-myb and cyclin D1 in HCC tissue may contribute to the proliferation of hepatoma cells, and have close correlation with the initiation and progression of HCC.
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Objective To establish the cell models of c-myb+/+ and c-myb+/-ES in vitro by gene targeting with ES cell culture system, with the aim to examine the detailed role of the transcription factor c-myb in haemopoietic commitment and differentiation. Methods Haemopoietic differentiation was initiated by seeding ES cells in LIF-free methylcellulose medium. The embryoids of c-myb+/+ and c-myb+/-ES were analyzed by methylcellulose colony assay and real-time PCR, the formative process of embryoids and the expression of relative genes were compared in each haemopoietic system at different differentiation stages and procedures. Results The formative process of embryoids was similar for both c-myb+/+ and c-myb+/-ES cells, but the size and frequency of EBs were reduced in the case of the c-myb+/-cells. Similar kinetics gain existed for the formation of CFU-Es in both groups, but the number in c-myb+/-group was less than that of c-myb+/+ group, and the colonies were generally smaller. BFU-E was first detectable on day 7, and the peak value emerged on day 10 in both groups. Similar kinetics gain existed for the formation of CFU-M in the two groups, but the number was larger in c-myb+/-group than that in c-myb+/+ group, while the number of CFU-GM in c-myb+/-group was less than that in c-myb+/+ group. Real-time PCR analysis showed no changes on the gene expressions of ?-globin, ?-globin, GATA3, CD34, Lys, c-fms and c-mpl in both groups. Conclusions Sub-level expressions of c-myb (55%) and c-myb+/-are sufficient to allow progenitor to expand, but they throw a negative influence on the terminal differentiation. Sub-level expression of c-myb may influence the erythropoiesis and granulocytic development, but throw no influence on the precursor cells to differentiate into macrophages. The expressive levels of c-myb have no effect on the expressions of ?-globin, ?-globin, GATA3, CD34, Lys, c-fms and c-mp.
