Bibliographical Mapping of Research into the Relationship between In Ovo Injection Practice and Hatchability in Poultry.

Recent advances in poultry practice have produced new tools enabling the poultry industry to increase productivity. Aiming at increasing production quality, varying protocols of in ovo injection facilitate the introduction of exogenous substances into the egg to complement the nutrients that support embryonic development up to hatching, which are already available in the internal and external compartments. Due to embryonic sensitivity, adding any substance into the egg can be either advantageous or disadvantageous for embryonic survival and can influence hatch rates. Thus, understanding the relationship between poultry practices and production rates is the first step towards successful commercial application. This review aims to assess the influence on hatch rates of injecting different substances in ovo, including effects on embryo and chick health parameters where these are reported. Bibliographic mappings of co-authorship of citations, co-occurrence of keywords, and bibliographic coupling based on the in ovo injection technique and hatchability parameters were also performed. Using the Scopus database, 242 papers were retrieved, reviewed, and submitted for bibliographic mapping using the VOSviewer® software. This review provides a broad overview of just over 38 years' research on the subject, revealing that studies have significantly increased and peaked in 2020, being produced primarily by US researchers and published primarily in the journal Poultry Science. It also reveals that despite negative reports relating to some substances in the embryo, in ovo delivery of substances may possibly change the poultry industry for the better in terms of production rates (hatchability) and/or poultry health.

Effects of Sanitizers on Microbiological Control of Hatching Eggshells and Poultry Health during Embryogenesis and Early Stages after Hatching in the Last Decade.

The sanitization of hatching eggs is the backbone of the hygienic-sanitary management of eggs on farms and extends to the hatchery. Poultry production gains depend on the benefits of sanitizers. Obtaining the maximum yield from incubation free of toxic sanitizers is a trend in poultry farming, closely following the concerns imposed through scientific research. The toxic characteristics of formaldehyde, the primary sanitizer for hatching eggs, are disappointing, but it is a cheap, practical and widely used antimicrobial. To overcome this shortcoming, multiple synthetic and natural chemical sanitizers have been, and continue to be, tested on hatching eggs. This review aims to evaluate the effects of different sanitizers on the microbiological quality of hatching eggshells and poultry health during embryogenesis and early stages after hatching.

Occurrence, Risk Factors, Serotypes, and Antimicrobial Resistance of Salmonella Strains Isolated from Imported Fertile Hatching Eggs, Hatcheries, and Broiler Farms in Trinidad and Tobago.

ABSTRACT: This cross-sectional study was conducted to determine the occurrence, risk factors, and characteristics of Salmonella isolates recovered from imported fertile broiler hatching eggs, hatcheries, and broiler farms in Trinidad and Tobago. Standard methods were used to isolate and characterize Salmonella isolates from two broiler hatcheries and 27 broiler farms in the country. The frequency of isolation of Salmonella was 0.0% for imported fertile hatching eggs (0 of 45 pools of 10 eggs each, i.e., 450 eggs), 7.6% for hatcheries (12 of 158 samples), and 2.8% for broiler farms (24 of 866 samples) (P = 0.006). Stillborn chicks at hatcheries had the highest prevalence of Salmonella (7 of 28 samples, 28.0%), whereas on broiler farms the cloacal swabs had the highest prevalence of Salmonella (15 of 675 samples, 2.2%). None of the 15 farm management and production practices investigated were significantly associated (P > 0.05) with the isolation of Salmonella. The predominant Salmonella serotypes were Kentucky (83.3%) and Infantis (62.5%) among hatchery and farm isolates, respectively. The disk diffusion method revealed frequencies of antimicrobial resistance (i.e., resistance to one or more agents) of 44.0% (11 of 25 isolates) and 87.5% (35 of 40 isolates) at hatcheries and broiler farms, respectively (P = 0.0002). Antimicrobial resistance among hatchery isolates was highest (28.0%) to doxycycline and kanamycin and was very high (>65%) among farm isolates to sulfamethoxazole-trimethoprim, gentamicin, ceftriaxone, kanamycin, and doxycycline. Multidrug resistance (MDR; i.e., resistance to antimicrobial agents from three or more classes) was exhibited by 4.0 and 85.7% of Salmonella isolates recovered from several environmental and animal sources at the hatcheries and farms, respectively (P < 0.0001). The high level of antimicrobial resistance and the presence of MDR among Salmonella isolates from broiler farms highlight the therapeutic implications and the potential for MDR strains to enter the food chain.

Spraying Hatching Eggs with Clove Essential Oil Does Not Compromise the Quality of Embryos and One-Day-Old Chicks or Broiler Performance.

The objective of this study was to evaluate whether sanitizing hatching eggs with clove essential oil in the preincubation phase affects broiler performance and influences the hatch window and quality of embryos and one-day-old chicks. Hatching eggs (n = 1280; mean weight = 58.64 ± 0.49 g) from a batch of 37-week-old broiler breeder hens of the CPK (Pesadão Vermelho) lineage were randomly distributed into four treatments in the preincubation phase. The treatments consisted of three different sanitization procedures (spraying with grain alcohol, spraying with clove essential oil, and fumigation with paraformaldehyde) and a control treatment (nonsanitized). The lengths of the embryos and one-day-old chicks (one of the parameters used to assess bird quality) were not significantly different among the treatments, with means of 15.30 ± 1.41 and 18.37 ± 0.76 mm, respectively. Body weight, body weight gain, feed consumption, and feed conversion rate in different rearing periods did not differ significantly among the treatments. However, there was a significant difference in the percentage of survivability during the initial period (1 to 28 days) among the treatments. In conclusion, clove essential oil treatment did not negatively affect the quality of embryos and one-day-old chicks or the performance of broilers.

Effects of a dry hydrogen peroxide disinfection system used in an egg cooler on hatchability and chick quality.

A sanitation method that could continually clean and disinfect the air and surfaces in a hatchery could provide a second layer of microbial reduction on top of routine cleaning and disinfection. A gaseous dry hydrogen peroxide (DHP) system has been used in other facilities for this purpose and could have potential for use in chicken hatcheries. Because the DHP is a true gas and can permeate through the entire hatchery space, contact with eggs during storage and incubation could potentially interfere with normal hatching processes. Therefore, the aim of this study was to evaluate the effects of the DHP system on hatching parameters and chick quality. A total of 3,960 hatching eggs were collected from an ∼40-week-old Ross 308 broiler breeder flock and distributed in 2 treatments: treated and nontreated. For the treated group, the egg cooler was cleaned, and 1 DHP generator was placed inside. Two other DHP generators were placed in the common area outside as well. Both areas were treated for 7 D before placement of eggs, and then eggs were collected and placed inside the cooler over a 4-day period. Eggs were then stored for an additional 3 D after the last collection. Dry hydrogen peroxide levels were recorded each day during storage. For the nontreated group, all DHP machines were removed from the cooler and external room, and the egg cooler was cleaned. Eggs were collected in the same way for the control group as the treated group. After storage, eggs were placed into a single stage Natureform incubator. The eggs exposed to DHP showed higher (P < 0.05) hatchability of fertile eggs and lower (P < 0.05) early embryonic dead than eggs from the nontreated group. No other parameters evaluated were different between groups. Based on this work, the DHP treatment of fertile eggs had no detrimental effect on any performance parameter, with potential positive effects seen on hatch of fertile eggs and early embryonic dead embryos.

The effects of using garlic extract for quail hatching egg disinfection on hatching results and performance

This experiment was carried out to determine the effects of using garlic extract (Allium sativum) as an alternative to formaldehyde for the disinfection of hatching eggs on hatching traits and growth performance of quails. Four treatments, with 240 hatching eggs each, were evaluated: egg immersion in two different levels (2.5% and 5.0%) of garlic extract (garlic-1, garlic-2), formaldehyde fumigation (positive control), and eggs not submitted to disinfection (negative control). Mid-term and late embryomortality, pipped egg, and culled chick rates were not influenced by the (p>0.05). The highest (p<0.05) early embryo mortality rate was detected in the formaldehyde treatment (9.99%), and the lowest in garlic-2 treatment (2.68%). Hatchability of fertile eggs was the lowest in the formaldehyde group (83.78%), followed by the control (85.20%), garlic-1 (87.11%) and garlic-2 (88.72%) groups, respectively. The highest final body weight (5 weeks of age) was obtained in the garlic-2 group (304.1 g), followed by the control (294.13 g), formaldehyde (290.56 g), and garlic-1 (288.44 g), respectively, and the differences were statistically significant (p<0.05). Feed intake and feed conversion ratio were not influenced by the treatments (p>0.05). The results obtained for hatching traits and live performance parameters indicated that the immersion of eggs in garlic extract may be used as an alternative to formaldehyde fumigation for the disinfection of hatching quail eggs.(AU)

The effects of using garlic extract for quail hatching egg disinfection on hatching results and performance

This experiment was carried out to determine the effects of using garlic extract (Allium sativum) as an alternative to formaldehyde for the disinfection of hatching eggs on hatching traits and growth performance of quails. Four treatments, with 240 hatching eggs each, were evaluated: egg immersion in two different levels (2.5% and 5.0%) of garlic extract (garlic-1, garlic-2), formaldehyde fumigation (positive control), and eggs not submitted to disinfection (negative control). Mid-term and late embryomortality, pipped egg, and culled chick rates were not influenced by the (p>0.05). The highest (p0.05). The results obtained for hatching traits and live performance parameters indicated that the immersion of eggs in garlic extract may be used as an alternative to formaldehyde fumigation for the disinfection of hatching quail eggs.

Gravidade específica de ovos de matrizes pesadas com diferentes idades no rendimento de incubação e no peso dos pintos pós-eclosão

SUMMARY The objective of this work was to evaluate the effect of specific gravity of eggs from broiler breeders of different ages in the yield of incubation and the weight of post-hatch chicks. Were used 3.072 eggs from two batches of broiler breeders, Cobb - 500®. The eggs were distributed a completely randomized design in a 2x4 factorial design with two broiler breeder ages (53 and 64 weeks) and four different egg densities (1.070, 1.075, 1.080, 1.085), totaling eight treatments and four replications (with 96 eggs per experimental unit). The evaluated variables were the egg weight (g), the hatching rate (%), the fertility rate (%), the weight loss of eggs during the incubation period (%) and the ratio of the weight of the chicks/egg weight (%). The results were submitted to analysis of variance, being that the effect of density was assessed by polynomial regression and the age effect of breeders by F test at 5% probability. Weight losses of eggs, except for the densities of 1,075 and 1,085, from breeders with 53 and 64 weeks of age, respectively, were within the values recommended in the literature. The chick weight was proportional to the weight of the egg, representing on average 68% and no differences between age and density of broiler breeders. The age of the breeders exerted influence on the yield of incubation, and the best results (p 0.05) obtained by eggs from breeders with 53 weeks of age.

RESUMO Objetivou-se neste trabalho avaliar o efeito da gravidade específica de ovos de matrizes pesadas com diferentes idades no rendimento de incubação e no peso dos pintos pós-eclosão. Foram utilizados 3.072 ovos oriundos de dois lotes de matrizes pesadas, linhagem Cobb 500®. Os ovos foram distribuídos em delineamento experimental inteiramente casualizado, em esquema fatorial 2x4, com duas idades de matrizes (53 e 64 semanas) e quatro diferentes densidades (1.070, 1.075, 1.080, 1.085), totalizando oito tratamentos e quatro repetições (com 96 ovos por unidade experimental). As variáveis avaliadas foram o peso do ovo (g), a taxa de eclosão (%), a taxa de fertilidade (%), a perda de peso dos ovos durante o período de incubação (%) e a relação do peso dos pintos/peso dos ovos (%). Os resultados foram submetidos à análise de variância, sendo que o efeito das densidades foi avaliado por meio de regressão polinomial e o efeito da idade das matrizes pelo teste F, ao nível de 5% de probabilidade. As perdas de peso dos ovos, exceto para as densidades de 1.075 e 1.085, oriundos de matrizes com 53 e 64 semanas de idade, respectivamente, apresentaram-se dentro dos valores recomendados na literatura. O peso do pinto ao nascer foi proporcional ao peso do ovo, representando em média 68% e sem diferenças entre a idade das matrizes e as diferentes densidades. A idade da matriz exerceu influência no rendimento de incubação, sendo as maiores taxas de fertilidade e eclosão total (p 0,05) obtida pelos ovos oriundos de matrizes com 53 semanas de idade.

Specific gravity of eggs of female broiler breeders in the yield of incubation and chick weight post-hatch / Gravidade específica de ovos de matrizes pesadas com diferentes idades no rendimento de incubação e no peso dos pintos pós-eclosão

The objective of this work was to evaluate the effect of specific gravity of eggs from broiler breeders of different ages in the yield of incubation and the weight of post-hatch chicks. Were used 3.072 eggs from two batches of broiler breeders, Cobb - 500®. The eggs were distributed a completely randomized design in a 2x4 factorial design with two broiler breeder ages (53 and 64 weeks) and four different egg densities (1.070, 1.075, 1.080, 1.085), totaling eight treatments and four replications (with 96 eggs per experimental unit). The evaluated variables were the egg weight (g), the hatching rate (%), the fertility rate (%), the weight loss of eggs during the incubation period (%) and the ratio of the weight of the chicks/egg weight (%). The results were submitted to analysis of variance, being that the effect of density was assessed by polynomial regression and the age effect of breeders by F test at 5% probability. Weight losses of eggs, except for the densities of 1,075 and 1,085, from breeders with 53 and 64 weeks of age, respectively, were within the values recommended in the literature. The chick weight was proportional to the weight of the egg, representing on average 68% and no differences between age and density of broiler breeders. The age of the breeders exerted influence on the yield of incubation, and the best results (p 0.05)(AU)

Objetivou-se neste trabalho avaliar o efeito da gravidade específica de ovos de matrizes pesadas com diferentes idades no rendimento de incubação e no peso dos pintos pós-eclosão. Foram utilizados 3.072 ovos oriundos de dois lotes de matrizes pesadas, linhagem Cobb 500®. Os ovos foram distribuídos em delineamento experimental inteiramente casualizado, em esquema fatorial 2x4, com duas idades de matrizes (53 e 64 semanas) e quatro diferentes densidades (1.070, 1.075, 1.080, 1.085), totalizando oito tratamentos e quatro repetições (com 96 ovos por unidade experimental). As variáveis avaliadas foram o peso do ovo (g), a taxa de eclosão (%), a taxa de fertilidade (%), a perda de peso dos ovos durante o período de incubação (%) e a relação do peso dos pintos/peso dos ovos (%). Os resultados foram submetidos à análise de variância, sendo que o efeito das densidades foi avaliado por meio de regressão polinomial e o efeito da idade das matrizes pelo teste F, ao nível de 5% de probabilidade. As perdas de peso dos ovos, exceto para as densidades de 1.075 e 1.085, oriundos de matrizes com 53 e 64 semanas de idade, respectivamente, apresentaram-se dentro dos valores recomendados na literatura. O peso do pinto ao nascer foi proporcional ao peso do ovo, representando em média 68% e sem diferenças entre a idade das matrizes e as diferentes densidades.(AU)

Specific gravity of eggs of female broiler breeders in the yield of incubation and chick weight post-hatch / Gravidade específica de ovos de matrizes pesadas com diferentes idades no rendimento de incubação e no peso dos pintos pós-eclosão

The objective of this work was to evaluate the effect of specific gravity of eggs from broiler breeders of different ages in the yield of incubation and the weight of post-hatch chicks. Were used 3.072 eggs from two batches of broiler breeders, Cobb - 500®. The eggs were distributed a completely randomized design in a 2x4 factorial design with two broiler breeder ages (53 and 64 weeks) and four different egg densities (1.070, 1.075, 1.080, 1.085), totaling eight treatments and four replications (with 96 eggs per experimental unit). The evaluated variables were the egg weight (g), the hatching rate (%), the fertility rate (%), the weight loss of eggs during the incubation period (%) and the ratio of the weight of the chicks/egg weight (%). The results were submitted to analysis of variance, being that the effect of density was assessed by polynomial regression and the age effect of breeders by F test at 5% probability. Weight losses of eggs, except for the densities of 1,075 and 1,085, from breeders with 53 and 64 weeks of age, respectively, were within the values recommended in the literature. The chick weight was proportional to the weight of the egg, representing on average 68% and no differences between age and density of broiler breeders. The age of the breeders exerted influence on the yield of incubation, and the best results (p 0.05)

Objetivou-se neste trabalho avaliar o efeito da gravidade específica de ovos de matrizes pesadas com diferentes idades no rendimento de incubação e no peso dos pintos pós-eclosão. Foram utilizados 3.072 ovos oriundos de dois lotes de matrizes pesadas, linhagem Cobb 500®. Os ovos foram distribuídos em delineamento experimental inteiramente casualizado, em esquema fatorial 2x4, com duas idades de matrizes (53 e 64 semanas) e quatro diferentes densidades (1.070, 1.075, 1.080, 1.085), totalizando oito tratamentos e quatro repetições (com 96 ovos por unidade experimental). As variáveis avaliadas foram o peso do ovo (g), a taxa de eclosão (%), a taxa de fertilidade (%), a perda de peso dos ovos durante o período de incubação (%) e a relação do peso dos pintos/peso dos ovos (%). Os resultados foram submetidos à análise de variância, sendo que o efeito das densidades foi avaliado por meio de regressão polinomial e o efeito da idade das matrizes pelo teste F, ao nível de 5% de probabilidade. As perdas de peso dos ovos, exceto para as densidades de 1.075 e 1.085, oriundos de matrizes com 53 e 64 semanas de idade, respectivamente, apresentaram-se dentro dos valores recomendados na literatura. O peso do pinto ao nascer foi proporcional ao peso do ovo, representando em média 68% e sem diferenças entre a idade das matrizes e as diferentes densidades.

Gravidade específica de ovos de matrizes pesadas com diferentes idades no rendimento de incubação e no peso dos pintos pós-eclosão

SUMMARY The objective of this work was to evaluate the effect of specific gravity of eggs from broiler breeders of different ages in the yield of incubation and the weight of post-hatch chicks. Were used 3.072 eggs from two batches of broiler breeders, Cobb - 500®. The eggs were distributed a completely randomized design in a 2x4 factorial design with two broiler breeder ages (53 and 64 weeks) and four different egg densities (1.070, 1.075, 1.080, 1.085), totaling eight treatments and four replications (with 96 eggs per experimental unit). The evaluated variables were the egg weight (g), the hatching rate (%), the fertility rate (%), the weight loss of eggs during the incubation period (%) and the ratio of the weight of the chicks/egg weight (%). The results were submitted to analysis of variance, being that the effect of density was assessed by polynomial regression and the age effect of breeders by F test at 5% probability. Weight losses of eggs, except for the densities of 1,075 and 1,085, from breeders with 53 and 64 weeks of age, respectively, were within the values recommended in the literature. The chick weight was proportional to the weight of the egg, representing on average 68% and no differences between age and density of broiler breeders. The age of the breeders exerted influence on the yield of incubation, and the best results (p 0.05) obtained by eggs from breeders with 53 weeks of age.

RESUMO Objetivou-se neste trabalho avaliar o efeito da gravidade específica de ovos de matrizes pesadas com diferentes idades no rendimento de incubação e no peso dos pintos pós-eclosão. Foram utilizados 3.072 ovos oriundos de dois lotes de matrizes pesadas, linhagem Cobb 500®. Os ovos foram distribuídos em delineamento experimental inteiramente casualizado, em esquema fatorial 2x4, com duas idades de matrizes (53 e 64 semanas) e quatro diferentes densidades (1.070, 1.075, 1.080, 1.085), totalizando oito tratamentos e quatro repetições (com 96 ovos por unidade experimental). As variáveis avaliadas foram o peso do ovo (g), a taxa de eclosão (%), a taxa de fertilidade (%), a perda de peso dos ovos durante o período de incubação (%) e a relação do peso dos pintos/peso dos ovos (%). Os resultados foram submetidos à análise de variância, sendo que o efeito das densidades foi avaliado por meio de regressão polinomial e o efeito da idade das matrizes pelo teste F, ao nível de 5% de probabilidade. As perdas de peso dos ovos, exceto para as densidades de 1.075 e 1.085, oriundos de matrizes com 53 e 64 semanas de idade, respectivamente, apresentaram-se dentro dos valores recomendados na literatura. O peso do pinto ao nascer foi proporcional ao peso do ovo, representando em média 68% e sem diferenças entre a idade das matrizes e as diferentes densidades. A idade da matriz exerceu influência no rendimento de incubação, sendo as maiores taxas de fertilidade e eclosão total (p 0,05) obtida pelos ovos oriundos de matrizes com 53 semanas de idade.