Carbon dots and gold nanoclusters assisted construction of a ratiometric fluorescent biosensor for detection of Gram-negative bacteria.

A core-satellite nanocomposite was prepared by encapsulating the photostable blue carbon dots (BCDs) in the core of silica as the reference signal readout, and the target-sensitive gold nanoclusters (AuNCs) covalently linked to the surface of silica as the respond signal readout. The nanocomposite (BCD@SiO2@AuNC) was used as a ratiometric fluorescent sensor to realize the selective detection of Gram-negative bacteria. The detection principle was based on the quenching of Cu2+ toward AuNCs and the reduction of Gram-negative bacteria toward Cu2+. The sensor exhibited good selectivity toward Gram-negative bacteria owing to the copper-homeostasis mechanism possessed by the bacteria. The sensor demonstrated linear response to the logarithm concentration of Gram-negative bacteria with determination coefficients higher than 0.912. The feasibility of the sensor was verified by analysis of Gram-negative bacteria in eggshell, swimming pool water, as well as Chinese cabbage samples with recoveries ranging from 93.9% to 109%.

[Research progress on mechanisms of bidirectional regulation of acupuncture].

Bidirectional regulation is one of the key function of acupuncture. The stimulator, mediator and receptor are the basis while the specificity of acupoints and the multi-target regulation of receptors receiving stimulation signals are the essential link of the bidirectional regulation of acupuncture. The possible mechanisms of bidirectional regulation of acupuncture are discussed in 4 aspects, i.e. homeostasis mechanism, stress reaction, central adaptive regulation and autonomic nerve regulation. Knowing the limitations of bidirectional regulation and exploring suitable researchmethods are proposed to be the key points in future researches.

Research progress on mechanisms of bidirectional regulation of acupuncture / 中国针灸

Bidirectional regulation is one of the key function of acupuncture. The stimulator, mediator and receptor are the basis while the specificity of acupoints and the multi-target regulation of receptors receiving stimulation signals are the essential link of the bidirectional regulation of acupuncture. The possible mechanisms of bidirectional regulation of acupuncture are discussed in 4 aspects, i.e. homeostasis mechanism, stress reaction, central adaptive regulation and autonomic nerve regulation. Knowing the limitations of bidirectional regulation and exploring suitable researchmethods are proposed to be the key points in future researches.