Pretreatment using Opuntia cochenillifera followed by household slow sand filters: technological alternatives for supplying isolated communities.

Household slow sand filter (HSSF) performance in continuous and intermittent flows was evaluated when influent water was treated with a natural coagulant extracted from Opuntia cochenillifera. The water under study, used as influent, had a turbidity of 111 ± 17.3 NTU. When clarifying the water with O. cochenillifera, the best condition obtained was 30  mg L-1 in natural pH (without correction), generating clarified water with turbidity satisfactory to filters operation (7.83 ± 2.32 NTU). The results indicated a better performance of continuous flow HSSF in turbidity removal (79.2% ± 8.39%) and higher efficiency of intermittent flow HSSF in the removal of E. coli (2.86 log ± 0.79 log for 12  h pause period and 2.41 log ± 0.42 log for 4  h pause period). For the sake of comparison, the evaluated HSSFs had the same production (60 L day-1). The impact on the interruption of the 96-h feed into the HSSFs was analysed and the results indicated a significant change in the quality of the filtered water after resuming the operation. This fragility of technology must be considered when it is implemented as lack of water can be a reality in the target communities. Acute toxicological assays with C. xanthus larvae showed no toxicity for pretreated and filtered water; however, more testing should be performed.

Hyperglycemia and dyslipidemia of Isabela, Galápagos, Ecuador: A pilot study of cardiovascular risk factors in an Isolated Island community.

AIM: To evaluate the prevalence of hyperglycemia and dyslipidemia in the population of Isabela, Galápagos, Ecuador, across gender and age (above or below 50). METHODS: In this population-based retrospective cross-sectional study among individuals in Isabela, Galápagos, Ecuador, demographic and metabolic factors were evaluated based on World Health Organization (WHO) Global Guidelines. RESULTS: The population overall exceeded the WHO guidelines for cardiovascular health. As to be expected, there was significance in the trend of increasing dyslipidemia and hyperglycemia with age except postprandial glucose. In those individuals below the age of 50, 8.0%, 49% and 26% had hyperglycemia, hypercholesterolemia and hypertriglyceridemia, respectively. However, in those above 50, they measured 24%, 68% and 36% respectively, showing a significant increase. CONCLUSIONS: Hyperglycemia and dyslipidemia appear to be prevalent in Isabela, Galápagos, Ecuador and this pilot study supports further research into metabolic syndrome and diabetes. Such data may help in healthcare planning and screening to ensure not only timely diagnosis, but prevention. The limitations of this data illustrate modalities that data collection can be improved, such as having a linked clinical history to the data itself and better patient follow up for such entities as post prandial glucose, for example. However, this pilot study presents a starting point for future directions of research, such as ascertaining prevalence of diabetes type II, metabolic syndrome and cardiovascular disease.

Human papillomavirus genotypes 68 and 58 are the most prevalent genotypes in women from quilombo communities in the state of Maranhão, Brazil.

OBJECTIVES: To determine the frequency of human papillomavirus (HPV) types and behavioral characteristics related to cytological abnormalities in women descendants of slaves, who live in isolated communities known as quilombos in the state of Maranhão, Brazil. METHODS: Cervicovaginal specimens of 353 women were analyzed by conventional cytology and genotyping. HPV detection and genotyping was performed using a linear array HPV genotyping test kit. Behavioral factors and their association with cytological abnormalities were analyzed, as well as the association between cytological abnormalities and HPV infection. RESULTS: The frequency of HPV infection was 13%, and infection with high-risk HPV types was more frequent than with low-risk types (10.2% vs. 2.8%). The most prevalent genotypes were HPV 68 (3.1%) and HPV 58 (2.6%). HPV-positive women were 6.5 times more likely than HPV-negative women to be diagnosed with cytological abnormalities. There was a significant association between HPV infection and the presence of cytological abnormalities in women 31-40 years of age and in women 51-60 years of age. CONCLUSIONS: A distinct profile of high-risk HPV genotypes was detected, with predominance of types 68 and 58. It is possible that the results of the present study are due to specific characteristics of the population, which is geographically isolated and maintains conservative sexual habits.