Enhancing Wound Healing: A Novel Topical Emulsion Combining CW49 Peptide and Lavender Essential Oil for Accelerated Regeneration and Antibacterial Protection.

Wound healing is a complex process involving blood cells, extracellular matrix, and parenchymal cells. Research on biomimetics in amphibian skin has identified the CW49 peptide from Odorrana grahami, which has been demonstrated to promote wound regeneration. Additionally, lavender essential oil exhibits anti-inflammatory and antibacterial activities. Given these considerations, we propose an innovative emulsion that combines the CW49 peptide with lavender oil. This novel formulation could serve as a potent topical treatment, potentially fostering the regeneration of damaged tissues and providing robust antibacterial protection for skin wounds. This study investigates the physicochemical properties, biocompatibility, and in vitro regenerative capacity of the active components and the emulsion. The results show that the emulsion possesses appropriate rheological characteristics for topical application. Both the CW49 peptide and lavender oil exhibit high viability in human keratinocytes, indicating their biocompatibility. The emulsion induces hemolysis and platelet aggregation, an expected behavior for such topical treatments. Furthermore, the lavender-oil emulsion demonstrates antibacterial activity against both Gram-positive and Gram-negative bacterial strains. Finally, the regenerative potential of the emulsion and its active components is confirmed in a 2D wound model using human keratinocytes. In conclusion, the formulated emulsion, which combines the CW49 peptide and lavender oil, shows great promise as a topical treatment for wound healing. Further research is needed to validate these findings in more advanced in vitro models and in vivo settings, potentially leading to improved wound-care management and novel therapeutic options for patients with skin injuries.

Effects of Lavender (Lavandula Angustifolia) Essential Oil on Fattening Performance, Meat Quality, Serum Antioxidant Enzymes, Gut Microbiota and Intestinal Histomorphology in Japanese Quails

This study examined the effects of lavender essential oil on performance, meat quality, microbial load, fatty acid profile and gut microbiota in quails. In the study, 200 quails (Coturnix coturnix Japonica) were divided into 4 groups and 5 subgroups. The groups consisted of a control group (0 mg/kg feed) and three lavender essential oil groups, namely Lav125 (125 mg/kg feed), Lav250 (250 mg/kg feed), and Lav500 (500 mg/kg feed). In terms of body weight change, Lav500 group had the best results after the control group (p>0.05). It was observed that, compared to the control group, pH levels were high (p<0.05) in Lav250 and Lav500 groups on the 9th day of storage. The effect of storage time on malondialdehyde (MDA) was used as a parameter of lipid peroxidation in meat, and the Lav250 and Lav500 groups presented lower concentrations as compared to the control group (p<0.05). In this study, the addition of lavender essential oil to the diet enriched the concentrations of n-3 and n-6 polyunsaturated fatty acids (PUFA). Moreover, the height of villi in the duodenum and jejunum, and consequently absorption, increased significantly in the Lav500 group as compared to the control group. The concentration of MDA, a blood serum antioxidant enzyme, decreased with the addition of lavender oil. Additionally, lavender essential oil added to quail diets was observed to increase the number of Lactobacillus spp. (probiotic bacteria) colonies (p<0.05).(AU)

Determinação do rendimento, teor e composição química de óleo essencial de espécie medicinal aloysia gratíssima (gillies & hook.) tronc. em diferentes horários do dia / Determination of yield, content and chemical composition of essential oil of medicinal plant aloysia gratissima (gillies & hook.) tronc. at different times of the day / Determinación del rendimiento, contenido y composición química del aceite esencial de la especie medicinal aloysia gratíssima (gillies & hook.) tronc. en distintos momentos del día

A Aloysia gratíssima, popularmente conhecida como alfazema-do-brasil é uma importante planta medicinal, sendo o óleo essencial rico em terpenos. Determinar o horário de colheita que resulte em maior rendimento, teor de óleo essencial e compostos químicos, são informações importantes para toda cadeia produtiva de plantas medicinais. Neste sentido, o objetivo do trabalho foi analisar o rendimento, teor e composição química do óleo essencial de A. gratíssima, em diferentes horários de colheita. O experimento foi realizado na Universidade Tecnológica Federal do Paraná - Campus Dois Vizinhos, nos anos de 2018 e 2019. Os tratamentos foram diferentes horários de colheita (9:00, 11:00, 15:00 e 17:00 horas) de massa fresca de A. gratíssima. O óleo essencial foi obtido pelo método de hidrodestilação e a composição química determinada por cromatografia gasosa-espectrometria de massa. Os melhores resultados de teor (1,18 e 0,55%) e rendimento de óleo essencial (0,71 e 0,33 g planta-1) foram obtidos nos horários de colheita das 9:00 e 11:00 horas, respectivamente, quando as plantas estavam no estádio de floração, e também as condições climáticas favoráveis, como temperaturas amenas. A cromatografia gasosa-espectrometria de massa revelou que os principais componentes dos óleos essenciais foram classificados como terpenos. Eucaliptol (39,30%) foi o componente majoritário do óleo essencial no horário de colheita das 9:00 horas. Os resultados desse estudo podem contribuir para otimizar o período de colheita de A. gratíssima, quanto à quantidade e qualidade dos óleos essenciais para indústria de produtos farmacêuticos e cosméticos.

Aloysia gratissima, popularly known as Brazilian lavender, is an important medicinal plant, the essential oil being rich in terpenes. Determining the harvest time that results in greater yield, essential oil content and chemical compounds, are important information for the entire production chain of medicinal plants In this sense, the objective of this work was to analyze the yield, content and chemical composition of the essential oil of A. gratíssima, at different harvest times. The experiment was carried out at the Universidade Tecnológica Federal do Paraná - Campus Dois Vizinhos, in the years 2018 and 2019. The treatments were different harvest times (9:00 am, 11:00 am, 3:00 pm and 5:00 pm) of fresh mass by A. gratissima. The essential oil was obtained by the hydrodistillation method and the chemical composition determined by gas chromatography-mass spectrometry. The best results for essential oil content (1.18 and 0.55%) and yield (0.71 and 0.33 g plant-1) were obtained at 9:00 am and 11:00 am, respectively, when the plants were in the flowering stage, and also favorable climatic conditions, such as mild temperatures. Gas chromatography-mass spectrometry revealed that the main components of essential oils were as terpenes. Eucalyptol (39.30%) was the major component of the essential oil at the 9:00 am harvest time. The results of this study may contribute to optimizing the period of harvesting A. gratissima, regarding the quantity and quality of essential oils for the pharmaceutical and cosmetics industry.

Aloysia gratissima, conocida popularmente como lavanda brasileña, es una importante planta medicinal, siendo el aceite esencial rico en terpenos. Determinar el tiempo de cosecha que resulte en mayor rendimiento, contenido de aceites esenciales y compuestos químicos, son información importante para toda la cadena productiva de las plantas medicinales. En ese sentido, el objetivo de este trabajo fue analizar el rendimiento, contenido y composición química del aceite esencial de A. gratíssima, en diferentes épocas de cosecha. El experimento se realizó en la Universidad Tecnológica Federal de Paraná - Campus Dois Vizinhos, en los años 2018 y 2019. Los tratamientos fueron diferentes tiempos de cosecha (9:00 am, 11:00 am, 3:00 pm y 5:00 pm ) de masa fresca por A. gratissima. El aceite esencial se obtuvo por el método de hidrodestilación y la composición química determinada por cromatografía de gases-espectrometría de masas. Los mejores resultados para contenido de aceite esencial (1.18 y 0.55%) y rendimiento (0.71 y 0.33 g planta-1) se obtuvieron a las 9:00 am y 11:00 am, respectivamente, cuando las plantas se encontraban en etapa de floración, y también condiciones climáticas favorables, como temperaturas suaves. La cromatografía de gases-espectrometría de masas reveló que los principales componentes de los aceites esenciales se clasificaron como terpenos. El eucaliptol (39,30%) fue el componente mayoritario del aceite esencial a las 9:00 a. m. del tiempo de cosecha. Los resultados de este estudio pueden contribuir a optimizar el período de cosecha de A. gratissima, en cuanto a la cantidad y calidad de los aceites esenciales para la industria farmacéutica y cosmética.

Inhaled Lavandula angustifolia essential oil enhances extinction learning and inhibits memory updating in mice submitted to the contextual fear conditioning.

ETHNOPHARMACOLOGICAL RELEVANCE: Lavender (Lavandula angustifolia) essential oil (EO) has a long history of use in emotional illness, including anxiety disorders. Cognitive mechanisms of learning and memory play a pivotal role in the etiology and maintenance of anxiety since exposure to cues related to aversive situations induces high arousal and anticipatory anxiety. Memory become labile after its reactivation and can be modulated by reconsolidation or extinction. Inhibition of memory reconsolidation or facilitation of memory extinction may be effective in preventing or minimizing the effect of contextual cues on anticipatory anxiety. AIM OF THE STUDY: We investigated the effect of Lavandula angustifolia EO in the memory updating of conditioned contextual fear. MATERIALS AND METHODS: Adult male C57Bl6 mice were submitted to fear conditioning. Two days after conditioning the mice underwent a reactivation session in a hybrid context and were then immediately exposed to vaporized water or essential oil at concentrations of 1%, 2.5% or 5% for 3 h. Two days later, the mice were tested in the original or an altered context and their freezing behavior was measured. In addition, mice were subjected to a fear memory recovery protocol followed by a reinstatement session. RESULTS: In the contextual fear test, 1% essential oil, but not 2.5% or 5%, reduced the freezing behavior response, whereas after a reinstatement session, exposure to 1% essential oil increased the freezing behavior response. CONCLUSIONS: These results suggest that Lavandula angustifolia essential oil enhances memory extinction and, consequently, inhibits memory updating.

Rural tourism marketing: Lavender tourism in Turkey / Marketing do turismo rural: Turismo lavanda na Turquia

This study aimed to comprehend rural tourism marketing through the visitors eyes and discuss its sustainability. What makes rural tourism marketing more sophisticated and complex is that it is a service marketing, associated with agricultural activities, natural conditions and rural areas, and the main providers have no experience in rural tourism or service marketing. The study investigated perception of visitors in a small village, Kuyucak, in the Southwest part of Turkey, where rural tourism based on lavender production has recently developed. Results of the analysis of 175 questionnaires completed by visitors online showed that more than half of the respondents had heard and got information about the village via social media. Although, the main purpose of their visit was stated as nature/rural experience and recreation/holiday, the primary motivation of the majority was taking and sharing photos via social media tools. Appearance of lavender and previous social media sharing are very important determinants in the decision-making and expectations of visitors. Results of the factor analysis showed that two components composed of the variables regarding pre-visit perception and the physical conditions of the village together explain sixty-five per cent of the overall satisfaction. The most important component in decision making of visitors was found their perception before visiting, which means that what they expected to see was more important than what they actually saw, which was represented by visitors satisfaction. Making visitors experience their pre-visit perception will contribute to the sustainability of rural tourism activities benefiting the area.(AU)

Este estudo teve como objetivo compreender o marketing do turismo rural através dos olhos dos visitantes e discutir sua sustentabilidade. O que torna o marketing do turismo rural mais sofisticado e complexo é que ele é um marketing de serviços, associado a atividades agrícolas, condições naturais e áreas rurais, e os principais provedores não têm experiência em turismo rural ou marketing de serviços. O estudo investigado a percepção de visitantes em uma pequena aldeia, Kuyucak, no sudoeste da Turquia, onde o turismo rural baseado na produção de lavanda se desenvolveu recentemente. Os resultados da análise de 175 questionários preenchidos pelos visitantes online mostraram que mais da metade dos entrevistados ouviram e receberam informações sobre o vilarejo pelas mídias sociais, e embora o objetivo principal de sua visita tenha sido declarado como natureza experiência rural e recreação feriado, a principal motivação da maioria foi tirar e compartilhar fotos através de ferramentas de mídia social. O surgimento da lavanda e o compartilhamento prévio de mídias sociais são determinantes muito importantes na tomada de decisões e expectativas dos visitantes. Os resultados da análise fatorial mostram que dois componentes compostos pelas variáveis relativas à percepção pré-visita e às condições físicas da aldeia, juntos, explicam 65% da satisfação geral. O que foi considerado interessante foi o componente sobre a percepção deles antes de visitar, o que significa que o que eles esperavam ver era mais importante do que o que eles realmente viram e encontraram o que foi representado pelo componente em evidências físicas sobre a satisfação dos visitantes. Fazer com que os visitantes experimentem sua percepção pré-visita contribuirá para a sustentabilidade das atividades de turismo rural e seus benefícios na área.(AU)

Rural tourism marketing: Lavender tourism in Turkey / Marketing do turismo rural: Turismo lavanda na Turquia

ABSTRACT: This study aimed to comprehend rural tourism marketing through the visitors' eyes and discuss its sustainability. What makes rural tourism marketing more sophisticated and complex is that it is a service marketing, associated with agricultural activities, natural conditions and rural areas, and the main providers have no experience in rural tourism or service marketing. The study investigated perception of visitors in a small village, Kuyucak, in the Southwest part of Turkey, where rural tourism based on lavender production has recently developed. Results of the analysis of 175 questionnaires completed by visitors online showed that more than half of the respondents had heard and got information about the village via social media. Although, the main purpose of their visit was stated as nature/rural experience and recreation/holiday, the primary motivation of the majority was taking and sharing photos via social media tools. Appearance of lavender and previous social media sharing are very important determinants in the decision-making and expectations of visitors. Results of the factor analysis showed that two components composed of the variables regarding pre-visit perception and the physical conditions of the village together explain sixty-five per cent of the overall satisfaction. The most important component in decision making of visitors was found their perception before visiting, which means that what they expected to see was more important than what they actually saw, which was represented by visitors' satisfaction. Making visitors experience their pre-visit perception will contribute to the sustainability of rural tourism activities benefiting the area.

RESUMO: Este estudo teve como objetivo compreender o marketing do turismo rural através dos olhos dos visitantes e discutir sua sustentabilidade. O que torna o marketing do turismo rural mais sofisticado e complexo é que ele é um marketing de serviços, associado a atividades agrícolas, condições naturais e áreas rurais, e os principais provedores não têm experiência em turismo rural ou marketing de serviços. O estudo investigado a percepção de visitantes em uma pequena aldeia, Kuyucak, no sudoeste da Turquia, onde o turismo rural baseado na produção de lavanda se desenvolveu recentemente. Os resultados da análise de 175 questionários preenchidos pelos visitantes online mostraram que mais da metade dos entrevistados ouviram e receberam informações sobre o vilarejo pelas mídias sociais, e embora o objetivo principal de sua visita tenha sido declarado como natureza experiência rural e recreação feriado, a principal motivação da maioria foi tirar e compartilhar fotos através de ferramentas de mídia social. O surgimento da lavanda e o compartilhamento prévio de mídias sociais são determinantes muito importantes na tomada de decisões e expectativas dos visitantes. Os resultados da análise fatorial mostram que dois componentes compostos pelas variáveis relativas à percepção pré-visita e às condições físicas da aldeia, juntos, explicam 65% da satisfação geral. O que foi considerado interessante foi o componente sobre a percepção deles antes de visitar, o que significa que o que eles esperavam ver era mais importante do que o que eles realmente viram e encontraram o que foi representado pelo componente em evidências físicas sobre a satisfação dos visitantes. Fazer com que os visitantes experimentem sua percepção pré-visita contribuirá para a sustentabilidade das atividades de turismo rural e seus benefícios na área.

Inhaled Lavandula angustifolia essential oil inhibits consolidation of contextual- but not tone-fear conditioning in rats.

Although the current treatment for anxiety is effective, it promotes a number of adverse reactions and medical interactions. Inhaled essential oils have a prominent action on the central nervous system, with minimal systemic effects, primarily because of reduced systemic bioavailability. The effects of drugs on the consolidation of fear conditioning reflects its clinical efficacy in preventing a vicious cycle of anticipatory anxiety leading to fearful cognition and anxiety symptoms. In this study, we investigated the effects of inhaled Lavandula angustifolia essential oil on the consolidation of aversive memories and its influence on c-Fos expression. Adult male Wistar rats were subjected to a fear conditioning protocol. Immediately after the training session, the rats were exposed to vaporized water or essential oil (1%, 2.5% and 5% solutions) for 4h. The next day, the rats underwent contextual- or tone-fear tests and 90min after the test they were euthanized and their brains processed for c-Fos immunohistochemistry. In the contextual-fear test, essential oil at 2.5% and 5% (but not 1%) reduced the freezing response and its respective c-Fos expression in the ventral hippocampus and amygdala. In the tone-fear test, essential oil did not reduce the freezing response during tone presentation. However, rats that inhaled essential oil at 2.5% and 5% (but not 1%) showed decreased freezing in the three minutes after tone presentation, as well as reduced c-Fos expression in the prefrontal cortex and amygdala. These results show that the inhalation of L. angustifolia essential oil inhibited the consolidation of contextual- but not tone-fear conditioning and had an anxiolytic effect in a conditioned animal model of anxiety.

A Systematic Review of the Anxiolytic-Like Effects of Essential Oils in Animal Models.

The clinical efficacy of standardized essential oils (such as Lavender officinalis), in treating anxiety disorders strongly suggests that these natural products are an important candidate source for new anxiolytic drugs. A systematic review of essential oils, their bioactive constituents, and anxiolytic-like activity is conducted. The essential oil with the best profile is Lavendula angustifolia, which has already been tested in controlled clinical trials with positive results. Citrus aurantium using different routes of administration also showed significant effects in several animal models, and was corroborated by different research groups. Other promising essential oils are Citrus sinensis and bergamot oil, which showed certain clinical anxiolytic actions; along with Achillea wilhemsii, Alpinia zerumbet, Citrus aurantium, and Spiranthera odoratissima, which, like Lavendula angustifolia, appear to exert anxiolytic-like effects without GABA/benzodiazepine activity, thus differing in their mechanisms of action from the benzodiazepines. The anxiolytic activity of 25 compounds commonly found in essential oils is also discussed.

Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill.

In the present study, the effects of two CaCl2.2H2O levels (440 and 1320 mg L-1) and two subcultures were evaluated on in vitro shoots of Lavandula angustifolia cv. Provence Blue. Ca2+ content of the apical, middle and basal portion of shoots was determined. Increasing CaCl2.2H2O level in the culture medium increased tissue Ca2+ content and decreased hyperhydricity. Shoot-tip necrosis also decreased with 1320 mg L-1 CaCl2.2H2O, but it did not occur in the second subculture. The middle and basal portion had higher Ca2+ content than apical portion. In non-hyperhydric tissues, there were smaller and more juxtaposed cells. Scanning electron microscopy of the leaves demonstrated that trichomes from in vitro leaf surface occurred in smaller quantities.

Microscopic characters of the leaf and stem of Lavandula dentata L. (Lamiaceae).

Lavandula dentata L. is an aromatic plant used in folk medicine for different purposes and, for this reason, phytochemical surveys have been carried out in the search for bioactive substances aiming to support its uses. Since there is little knowledge on the structural aspects of L. dentata, this work has studied the anatomical characters of the leaf and stem using light and scanning electron microscopy, in order to assist the species identification. As a result, there are different types of trichomes: capitate glandular with uni- or bicellular head, peltate glandular with multicellular head, and branched non-glandular. The leaf is hypostomatic showing diacytic stomata. The epidermis is uniseriate and coated with striate cuticle. The mesophyll is dorsiventral and the midrib is concave-convex and traversed by a single collateral vascular bundle. The stem is quadrangular and has alternating strands of collenchyma and cortical parenchyma as well as a typical endodermis in the cortex. The phloem and xylem cylinders are traversed by narrow rays and there is an incomplete sclerenchymatic sheath adjoining the phloem. These results are a novelty for the species and contribute to distinguish it from other lavenders.

Indução de enraizamento em diferentes acessos de Lavandula angustifolia por meio de aplicação de auxina / Rooting induction of different Lavandula angustifolia accessions by auxin application

A Lavandula angustifolia Miller (alfazema), considerada de difícil enraizamento, apresenta muitas variedades e formas horticulturais, e genótipos de diferentes procedências podem apresentar variação na sua capacidade de enraizamento. O objetivo deste experimento foi testar diferentes concentrações de ácido indol butírico (AIB) no enraizamento de oito genótipos (G1 a G8) de L. angustifolia de diferentes procedências. Estacas foram tratadas com diferentes doses (0 a 2000 mg L-1) de AIB.  Porcentagem de enraizamento (RN), número de raízes (RN) e comprimento da maior raiz (RL) foram avaliados. Os diferentes acessos apresentaram diferentes respostas à aplicação de AIB, que não influenciou no enraizamento de G1, G2 e G7; foi benéfica para G3 até 700 mg L-1; benéfica para G4 e G6; e prejudicial para G5 e G8; a mesma dose de AIB que promoveu melhor enraizamento em G3 (700 mg L-1) foi tóxica para G5; 1500 mg L-1 de AIB promoveu melhor enraizamento, maior número e comprimento de raízes em G6 mas foi completamente e significativamente prejudicial para G8. G1 e G3 apresentaram altas taxas de enraizamento, apesar da L. angustifolia ser considerada espécie de difícil enraizamento. G4, G5 e G6, todavia, apresentaram enraizamento deficiente quando comparados aos outros genótipos. A necessidade de aplicação de auxina e a dose adequada não podem ser generalizadas para diferentes acessos de L. an

Lavandula angustifolia Miller (lavender), considered a hard to root species, is comprised of many varieties and horticultural forms, and genotypes from different provenances may present variance in rooting capacity. The objective of this experiment was to test different concentrations of indol-3-butyric acid (IBA) on rooting of eight (G1 to G8) L. angustifolia genotypes from different provenances. Cuttings were treated with different doses (0 to 2000 mg L-1) of IBA. Rooting percentage (RP), root number (RN), and length of the longest root (RL) were evaluated. The different accessions presented very diverse response to IBA application. IBA did not exert influence on rooting of G1, G2, and G7; was benefic to G3 until 700 mg L-1; was benefic to G4 and G6; and was prejudicial to G5 and G8; the same IBA dose which promoted better rooting on G3 (700 mg L-1) was toxic to G5; 1500 mg L-1 of IBA promoted better rooting, greater root number and length on G6 but was completely and significantly detrimental to G8. G1 and G3 presented high rooting percentages, in spite of L. angustifolia being considered a hard to root species. G4, G5 and G6, however, presented very poor rooting, compared to the other genotypes. Necessity of auxin application and adequate dose may not be generalized to L. angustifolia different accessions. Its use should be incentivized when the genotype is recalcitrant to

Influence of cutting type and size on rooting of Lavandula dentata L / Influência do tipo e tamanho de estaca no enraizamento de Lavandula dentata L

Even though the Lavandula species may be propagated by seeds, it should not be the preferred propagation method because it causes a great lack of uniformity. On the other hand, asexually propagated lavender crops would provide more homogeneous crops, and clones from high quality plant material would increase the odds for obtaining a higher quality essential oil. However, problems such as poor rooting and restrict market availability for superior clones have been a problem in vegetative propagation of the Lavandula species. The objective of this work was to define which type and size of cutting is more adequate for cutting propagation of L. dentata, a very productive Lavandula species. Cuttings with 5, 8, 10 or 13 cm and from the apical or basal parts of stems cut from L. dentata stock plants were placed in Plantmax HT® filled polystyrene foam trays and kept under intermittent mist system for two months. Averages of root number, length of the longest root, fresh and dry root weight, and percentage of rooting were evaluated. Apical cuttings combined 97.9% rooting with an average of 13.2 roots per cutting and basal cuttings 93.7% rooting with 2.98 roots per cutting. Apical cuttings with at least 10 cm in length were considered the most adequate for cutting propagation of L. dentata.

Apesar de serem propagadas por sementes, as espécies de Lavandula deveriam ser preferencialmente propagadas por estaquia devido a grande falta de uniformidade causada pela primeira, enquanto que a propagação assexual da alfazema permite maior homogeneidade da lavoura e o uso de clones de alta qualidade aumentaria as chances de se obter óleo essencial de alta qualidade. No entanto, o baixo poder de enraizamento e o mercado restrito de clones superiores têm sido problemas para a propagação vegetativa de espécies de alfazema. O objetivo deste trabalho foi definir qual tipo e tamanho de estaca seriam mais adequados para a propagação de L. dentata, espécie muito produtiva de Lavandula, por estaquia. Estacas com 5, 8, 10 ou 13 cm das partes apical e basal de ramos de plantas matrizes de L. dentata foram colocados em bandejas de isopor com o substrato Plantmax HT® e mantidas em casa de vegetação sob nebulização por dois meses. As médias de número de raízes, comprimento da raiz mais longa, massa fresca e seca de raízes e porcentagem de enraizamento foram avaliadas. Estacas apicais apresentaram enraizamento de 97,9% e 13,2 raízes por estaca em média e as estacas basais 93,7% de enraizamento e 2,98 raízes por estaca. Estacas apicais com pelo menos 10 cm de comprimento foram consideradas as mais adequadas para a propagação da L. dentata por estaquia.

Indução de enraizamento em diferentes acessos de Lavandula angustifolia por meio de aplicação de auxina / Rooting induction of different Lavandula angustifolia accessions by auxin application

A Lavandula angustifolia Miller (alfazema), considerada de difícil enraizamento, apresenta muitas variedades e formas horticulturais, e genótipos de diferentes procedências podem apresentar variação na sua capacidade de enraizamento. O objetivo deste experimento foi testar diferentes concentrações de ácido indol butírico (AIB) no enraizamento de oito genótipos (G1 a G8) de L. angustifolia de diferentes procedências. Estacas foram tratadas com diferentes doses (0 a 2000 mg L-1) de AIB.  Porcentagem de enraizamento (RN), número de raízes (RN) e comprimento da maior raiz (RL) foram avaliados. Os diferentes acessos apresentaram diferentes respostas à aplicação de AIB, que não influenciou no enraizamento de G1, G2 e G7; foi benéfica para G3 até 700 mg L-1; benéfica para G4 e G6; e prejudicial para G5 e G8; a mesma dose de AIB que promoveu melhor enraizamento em G3 (700 mg L-1) foi tóxica para G5; 1500 mg L-1 de AIB promoveu melhor enraizamento, maior número e comprimento de raízes em G6 mas foi completamente e significativamente prejudicial para G8. G1 e G3 apresentaram altas taxas de enraizamento, apesar da L. angustifolia ser considerada espécie de difícil enraizamento. G4, G5 e G6, todavia, apresentaram enraizamento deficiente quando comparados aos outros genótipos. A necessidade de aplicação de auxina e a dose adequada não podem ser generalizadas para diferentes acessos de L. an

Lavandula angustifolia Miller (lavender), considered a hard to root species, is comprised of many varieties and horticultural forms, and genotypes from different provenances may present variance in rooting capacity. The objective of this experiment was to test different concentrations of indol-3-butyric acid (IBA) on rooting of eight (G1 to G8) L. angustifolia genotypes from different provenances. Cuttings were treated with different doses (0 to 2000 mg L-1) of IBA. Rooting percentage (RP), root number (RN), and length of the longest root (RL) were evaluated. The different accessions presented very diverse response to IBA application. IBA did not exert influence on rooting of G1, G2, and G7; was benefic to G3 until 700 mg L-1; was benefic to G4 and G6; and was prejudicial to G5 and G8; the same IBA dose which promoted better rooting on G3 (700 mg L-1) was toxic to G5; 1500 mg L-1 of IBA promoted better rooting, greater root number and length on G6 but was completely and significantly detrimental to G8. G1 and G3 presented high rooting percentages, in spite of L. angustifolia being considered a hard to root species. G4, G5 and G6, however, presented very poor rooting, compared to the other genotypes. Necessity of auxin application and adequate dose may not be generalized to L. angustifolia different accessions. Its use should be incentivized when the genotype is recalcitrant to

Fitoaromaterapia como complemento para mejorar la salud laboral / Supplementary phytoaromatherapy in occupational health improvement

Introducción: la fitoaromaterapia es una terapia complementaria que utiliza los aceites esenciales contenidos en órganos especializados de las plantas. Uno de los efectos atribuibles a algunas esencias es el ansiolítico, presente en especies como la lavanda (Lavandula officinalis L., Labiateae), una de las más estudiadas a nivel mundial. No obstante, en Chile no existen registros de estudios que evalúen el efecto ansiolítico de esencias en poblaciones susceptibles. El estrés laboral se conceptualiza como el conjunto de fenómenos que suceden en el organismo del trabajador, con la participación de agentes estresantes lesivos derivados directamente del trabajo o que con motivo de este, pueden afectar la salud y el rendimiento del trabajador. Objetivo: evaluar los efectos ansiolíticos de la lavanda, como una terapia complementaria no invasiva en el tratamiento de la ansiedad y la percepción de afectividad y bienestar psicológico en trabajadores voluntarios de la Universidad de Concepción. Métodos: la esencia se obtuvo por hidrodestilación en aparato clavenger y su caracterización química se realizó por CG-MS. Para evaluar el efecto ansiolítico se utilizaron 3 instrumentos: beck anxiety inventory (BAI), escala de afectos positivos y negativos (PANAS) y psychological general well-being index (PGWB). Resultados: el análisis químico de la esencia evidenció presencia de compuestos con reconocida actividad ansiolítica, como el linalol y el acetato de linalilo. Con respecto a la percepción de los funcionarios, después de aplicar la intervención con lavanda, se observó una reducción de la ansiedad, al mismo tiempo hubo una mejoría en el bienestar psicológico general en relación con la ansiedad, salud general y vitalidad, y en la afectividad negativa; mientras que en la afectividad positiva no hubo cambios significativos. Conclusiones: la terapia sobre la base de aromas naturales, como el de la lavanda, constituye un método simple y barato de mejorar la calidad de vida de la población susceptible a desarrollar diversas enfermedades producto del estrés diario.

Introduction: phytoaromatherapy is a supplementary treatment using essential oils found in specific parts of plants. One of the effects attributable to certain types of essences such as lavender (Lavandula officinalis L., Labiateae), widely studied around the world, is the anti-ansiolytic impact. There are no studies in Chile of the anti anxiety effect of this essence in sensitive populations. Work-related stress has been defined as a series of events that occur in a worker's body in which work-related harmful stressing agents are involved and may affect health and work performance Objective: to evaluate the anxiolytic effects of Lavender as a non-invasive supplementary therapy in the treatment of anxiety and on the perception of affectivity, and general psychological well-being in voluntary group of workers from the University of Concepción. Methods: the essential oil was obtained by hydrodistillation in Clavenger equipment and the chemical characterization was performed by CG-MS. To evaluate the anxiolytic effects, three instruments: Beck Anxiety Inventory (BAI), Positive and Negative Affect Scale (PANAS) and Psychological General Well-Being Index (PGWB Index) were used. Results: the chemical analysis of the essence evinced existence of recognized anxiolytic action compounds such as linalol and linalilo acetate. After applying the lavender, workers reported lower levels of anxiety, and improvement of their general psychological well-being in terms of anxiety, global health condition and strength and negative affect whereas no significant changes were seen in positive affect. Conclusions: the natural aroma-based therapy constitutes a simple and inexpensive method of increasing the quality of life of the population sensitive to several daily stress-derived diseases.

Teor e composição do óleo essencial de inflorescências e folhas de Lavandula dentata L. em diferentes estádios de desenvolvimento floral e épocas de colheita / Yield and composition of essential oil from inflorescences and leaves of lavender (Lavandula dentata L.) in different flower development stages and harvest times

O óleo essencial de Lavandula dentata L. possui importância econômica devido à utilização nas indústrias farmacêutica, cosmética e de higiene pessoal. O presente trabalho teve como objetivo avaliar o teor e a composição do óleo essencial de inflorescências e folhas de lavanda em diferentes estádios de desenvolvimento. O delineamento experimental foi inteiramente casualizado em esquema fatorial 2 x 3, sendo três estádios de desenvolvimento floral (botão, pré-antese/antese e senescente) e duas épocas de colheita (janeiro e abril), com cinco repetições. As amostras de óleo essencial foram obtidas por hidrodestilação, sendo os constituintes analisados por cromatografia em fase gasosa acoplada à espectrometria de massas (CG/EM). Houve interação entre os fatores épocas de colheita e desenvolvimento floral no teor de óleo essencial das inflorescências, sendo observada média superior em botões florais colhidos em janeiro. Os estádios de desenvolvimento não alteraram o teor do óleo essencial das folhas. Os estádios de desenvolvimento influenciaram nos teores dos constituintes dos óleos essenciais das inflorescências e folhas. No óleo essencial em estádio de flor senescente da colheita de abril, observou-se teor elevado de 1,8-cineol, enquanto nos demais estádios de desenvolvimento os teores foram menores em ambas as épocas de colheita. Teores elevados de cânfora foram observados nos estádios de pré-antese/antese e senescente, na colheita de janeiro. O óleo essencial das folhas apresentou teor superior de 1,8-cineol em ramos com flores em estádio de pré-antese/antese. Os teores de cânfora e fenchona foram superiores em ramos contendo botões. O óleo essencial das folhas de ramos com botões e flores senescentes apresentou teor superior de linalol em relação ao óleo essencial das inflorescências.

The essential oil of Lavandula dentata L. has economic importance due to its utilization by pharmaceutical, cosmetic and personal care industries. The aim of this study was to evaluate the yield and composition of essential oil from lavender inflorescences and leaves in different development stages. The experimental design was completely randomized in 2 x 3 factorial arrangement, with three flower development stages (bud, pre-anthesis/anthesis and senescence) and two harvest periods (January and April), and 5 replicates. The essential oil samples were obtained by hydrodistillation, and the compounds were analyzed by gas chromatography coupled to mass spectrometry (GC/MS). There was an interaction between the factors harvest time and flower development on essential oil yield of inflorescences, and a superior average was observed for flower buds harvested in January. The development stages did not alter the essential oil yield of leaves. The development stages influenced the levels of compounds of the essential oil from inflorescences and leaves. For essential oil in senescent flower stage harvested in April, there was a high level of 1,8-cineol, whereas in the remaining development stages, the levels were lower in both harvest times. The levels of camphor increased in the pre-anthesis/anthesis and senescence in January harvest. The essential oil from leaves presented higher levels of 1,8-cineol in branches with flowers in pre-anthesis/anthesis. Camphor and fenchone levels were higher in branches with buds. The essential oil from leaves of branches with buds and senescent flowers showed higher levels of linalool than that from inflorescences.

Adventitious rooting of auxin-treated Lavandula dentata cuttings / Enraizamento adventício de estacas de Lavandula dentata tratadas com auxina

Lavandula species may be propagated by seeds. However, plants will present expressive variation both in size and essential oil content. Indole-3-butyric acid (IBA) is a growth regulator which efficiently stimulates rooting and the objective of this research was to observe the influence of different IBA concentrations on rooting of L. dentata cuttings. Herbaceous cuttings with approximately 10cm in length and around 1/3 of leaf retention had their bases submerged into 0 (control), 500, 1.000, 2.000 or 3.000mg dm-3 of IBA diluted in de-ionized water for 30 seconds and placed in polystyrene foam trays filled with commercial substrate and kept under intermittent mist system. Averages of root number, length of the longest root, fresh and dry root weight, and percentage of rooted cuttings were evaluated after 60 days. There was no significant difference between treatments for the length of root, fresh and dry root weight. The percentage of rooted cuttings increased with IBA concentrations. A quadratic regression was obtained to root number. The 2.000mg dm-3 dose increased the percentage of rooted cuttings and the mean root number per cutting. L. dentata cuttings are easy to root.

Espécies de Lavandula podem ser propagadas por sementes. Entretanto, as plantas apresentarão elevada variabilidade em tamanho e em produção de óleo essencial. O ácido indol-3-butírico (AIB) é um regulador de crescimento que eficientemente estimula o enraizamento. O objetivo deste trabalho foi observar a influência de diferentes concentrações de AIB no enraizamento de estacas de L. dentata. Estacas herbáceas com aproximadamente 10cm de comprimento e 1/3 de retenção foliar tiveram suas bases submersas em 0 (controle), 500, 1.000, 2.000 ou 3.000mg dm-3 de AIB diluído em água deionizada por 30 segundos e colocadas em bandejas de isopor contendo substrato comercial sob sistema de nebulização intermitente. As médias de número de raízes, comprimento da raiz mais longa, massa fresca e seca de raízes e porcentagem de estacas enraizadas foram avaliadas após 60 dias. Não houve diferença significativa entre os tratamentos para o comprimento da raiz e a massa fresca e seca de raízes. A porcentagem de estacas enraizadas aumentou com a concentração de AIB. Uma regressão quadrática foi obtida para o número de raízes. A concentração de 2.000mg dm-3 aumentou a porcentagem de estacas enraizadas e o número de raízes formadas por estaca. As estacas de L. dentata são de fácil enraizamento.

Adventitious rooting of auxin-treated Lavandula dentata cuttings

Lavandula species may be propagated by seeds. However, plants will present expressive variation both in size and essential oil content. Indole-3-butyric acid (IBA) is a growth regulator which efficiently stimulates rooting and the objective of this research was to observe the influence of different IBA concentrations on rooting of L. dentata cuttings. Herbaceous cuttings with approximately 10cm in length and around 1/3 of leaf retention had their bases submerged into 0 (control), 500, 1.000, 2.000 or 3.000mg dm-3 of IBA diluted in de-ionized water for 30 seconds and placed in polystyrene foam trays filled with commercial substrate and kept under intermittent mist system. Averages of root number, length of the longest root, fresh and dry root weight, and percentage of rooted cuttings were evaluated after 60 days. There was no significant difference between treatments for the length of root, fresh and dry root weight. The percentage of rooted cuttings increased with IBA concentrations. A quadratic regression was obtained to root number. The 2.000mg dm-3 dose increased the percentage of rooted cuttings and the mean root number per cutting. L. dentata cuttings are easy to root.

Espécies de Lavandula podem ser propagadas por sementes. Entretanto, as plantas apresentarão elevada variabilidade em tamanho e em produção de óleo essencial. O ácido indol-3-butírico (AIB) é um regulador de crescimento que eficientemente estimula o enraizamento. O objetivo deste trabalho foi observar a influência de diferentes concentrações de AIB no enraizamento de estacas de L. dentata. Estacas herbáceas com aproximadamente 10cm de comprimento e 1/3 de retenção foliar tiveram suas bases submersas em 0 (controle), 500, 1.000, 2.000 ou 3.000mg dm-3 de AIB diluído em água deionizada por 30 segundos e colocadas em bandejas de isopor contendo substrato comercial sob sistema de nebulização intermitente. As médias de número de raízes, comprimento da raiz mais longa, massa fresca e seca de raízes e porcentagem de estacas enraizadas foram avaliadas após 60 dias. Não houve diferença significativa entre os tratamentos para o comprimento da raiz e a massa fresca e seca de raízes. A porcentagem de estacas enraizadas aumentou com a concentração de AIB. Uma regressão quadrática foi obtida para o número de raízes. A concentração de 2.000mg dm-3 aumentou a porcentagem de estacas enraizadas e o número de raízes formadas por estaca. As estacas de L. dentata são de fácil enraizamento.

Adventitious rooting of auxin-treated Lavandula dentata cuttings

Lavandula species may be propagated by seeds. However, plants will present expressive variation both in size and essential oil content. Indole-3-butyric acid (IBA) is a growth regulator which efficiently stimulates rooting and the objective of this research was to observe the influence of different IBA concentrations on rooting of L. dentata cuttings. Herbaceous cuttings with approximately 10cm in length and around 1/3 of leaf retention had their bases submerged into 0 (control), 500, 1.000, 2.000 or 3.000mg dm-3 of IBA diluted in de-ionized water for 30 seconds and placed in polystyrene foam trays filled with commercial substrate and kept under intermittent mist system. Averages of root number, length of the longest root, fresh and dry root weight, and percentage of rooted cuttings were evaluated after 60 days. There was no significant difference between treatments for the length of root, fresh and dry root weight. The percentage of rooted cuttings increased with IBA concentrations. A quadratic regression was obtained to root number. The 2.000mg dm-3 dose increased the percentage of rooted cuttings and the mean root number per cutting. L. dentata cuttings are easy to root.

Espécies de Lavandula podem ser propagadas por sementes. Entretanto, as plantas apresentarão elevada variabilidade em tamanho e em produção de óleo essencial. O ácido indol-3-butírico (AIB) é um regulador de crescimento que eficientemente estimula o enraizamento. O objetivo deste trabalho foi observar a influência de diferentes concentrações de AIB no enraizamento de estacas de L. dentata. Estacas herbáceas com aproximadamente 10cm de comprimento e 1/3 de retenção foliar tiveram suas bases submersas em 0 (controle), 500, 1.000, 2.000 ou 3.000mg dm-3 de AIB diluído em água deionizada por 30 segundos e colocadas em bandejas de isopor contendo substrato comercial sob sistema de nebulização intermitente. As médias de número de raízes, comprimento da raiz mais longa, massa fresca e seca de raízes e porcentagem de estacas enraizadas foram avaliadas após 60 dias. Não houve diferença significativa entre os tratamentos para o comprimento da raiz e a massa fresca e seca de raízes. A porcentagem de estacas enraizadas aumentou com a concentração de AIB. Uma regressão quadrática foi obtida para o número de raízes. A concentração de 2.000mg dm-3 aumentou a porcentagem de estacas enraizadas e o número de raízes formadas por estaca. As estacas de L. dentata são de fácil enraizamento.

Adventitious rooting of auxin-treated Lavandula dentata cuttings

Lavandula species may be propagated by seeds. However, plants will present expressive variation both in size and essential oil content. Indole-3-butyric acid (IBA) is a growth regulator which efficiently stimulates rooting and the objective of this research was to observe the influence of different IBA concentrations on rooting of L. dentata cuttings. Herbaceous cuttings with approximately 10cm in length and around 1/3 of leaf retention had their bases submerged into 0 (control), 500, 1.000, 2.000 or 3.000mg dm-3 of IBA diluted in de-ionized water for 30 seconds and placed in polystyrene foam trays filled with commercial substrate and kept under intermittent mist system. Averages of root number, length of the longest root, fresh and dry root weight, and percentage of rooted cuttings were evaluated after 60 days. There was no significant difference between treatments for the length of root, fresh and dry root weight. The percentage of rooted cuttings increased with IBA concentrations. A quadratic regression was obtained to root number. The 2.000mg dm-3 dose increased the percentage of rooted cuttings and the mean root number per cutting. L. dentata cuttings are easy to root.

Espécies de Lavandula podem ser propagadas por sementes. Entretanto, as plantas apresentarão elevada variabilidade em tamanho e em produção de óleo essencial. O ácido indol-3-butírico (AIB) é um regulador de crescimento que eficientemente estimula o enraizamento. O objetivo deste trabalho foi observar a influência de diferentes concentrações de AIB no enraizamento de estacas de L. dentata. Estacas herbáceas com aproximadamente 10cm de comprimento e 1/3 de retenção foliar tiveram suas bases submersas em 0 (controle), 500, 1.000, 2.000 ou 3.000mg dm-3 de AIB diluído em água deionizada por 30 segundos e colocadas em bandejas de isopor contendo substrato comercial sob sistema de nebulização intermitente. As médias de número de raízes, comprimento da raiz mais longa, massa fresca e seca de raízes e porcentagem de estacas enraizadas foram avaliadas após 60 dias. Não houve diferença significativa entre os tratamentos para o comprimento da raiz e a massa fresca e seca de raízes. A porcentagem de estacas enraizadas aumentou com a concentração de AIB. Uma regressão quadrática foi obtida para o número de raízes. A concentração de 2.000mg dm-3 aumentou a porcentagem de estacas enraizadas e o número de raízes formadas por estaca. As estacas de L. dentata são de fácil enraizamento.