Discovery and lectotype designation of Longitarsuscalifornicus (Motschulsky) (Coleoptera, Chrysomelidae, Galerucinae, Alticini).

The lectotype of Longitarsuscalifornicus (Motschulsky, 1845) is designated, described, and illustrated. An illustrated key to eight light-colored Longitarsus species known to occur in the western United States is presented. A brief history of Russian entomological collecting in North America during the first half of 19th century, with specimens preserved in Zoological Museum of Moscow University, Moscow and Zoological Institute, St. Petersburg, is provided.

Feeding Preferences of the Bean Leaf Beetle (Ootheca spp.) (Coleoptera: Chrysomelidae): Insights for Targeted Pest Control Strategies in Uganda.

The bean leaf beetle (BLB) (Ootheca spp.) is a polyphagous pest causing significant yield losses in Uganda, particularly in the Northern and Eastern regions on various hosts plants. Despite its polyphagous behaviour, the BLB exhibits preferential feeding, offering an opportunity for targeted pest management. This study explored its feeding preferences across seven crops: common bean, cowpea, greengram, okra, roselle (malakwang), groundnuts, and soybean. This study was conducted in Arua and Lira districts using a randomized complete block design for two rainy seasons (2018A and 2018B). The results showed significant differences in BLB abundance and foliar damage among host crops, locations, days after planting and seasons. Cowpea was the most preferred crop while groundnuts was the least preferred. Therefore, cowpea can be recommended for use as a trap for managing Ootheca spp. in gardens where it is not the main crop. There was a higher pest abundance in Arua than in Lira. There was also a higher pest abundance in 2018A than in 2018B. These findings highlight the importance of understanding BLB's feeding preferences for implementing effective IPM strategies, emphasizing the potential role of trap cropping, especially with cowpea, to minimize BLB damage in resource-constrained agricultural settings.

Two new species of Hesperopenna Medvedev & Dang, 1981 (Coleoptera, Chrysomelidae, Galerucinae) from Singapore.

Two new species of Hesperopenna Medvedev & Dang, 1981 are described from Singapore: H.temaseksp. nov. and H.bakerisp. nov. The specimens of both new species were collected by Charles Fuller Baker and found in the unidentified Galerucinae material deposited in the National Museum of Natural History, Smithsonian Institution, Washington, DC. Hesperopennatemaseksp. nov. is diagnosed by the black extreme elytral suture in the basal third, antennae longer than the body, the structure of the penis, and the last abdominal ventrite with two deep U-shaped incisions in females. Hesperopennabakerisp. nov. is diagnosed by the black tibia and first two tarsomeres, and the structure of the penis.

A Review of Chinese Species of the Genus Oides Weber, 1801 (Coleoptera: Chrysomelidae: Galerucinae).

In this study, 25 species of Oides Weber from China were reviewed. Among them, the following seven new species are described: Oides angustasp. nov., O. cystoprocessasp. nov., O. paraborerisp. nov., O. parabowringiisp. nov., O. parathibettanasp. nov., O. shimenensissp. nov., and O. yunnanensissp. nov.; Oides innocua Gahan has been recorded in China for the first time. A key to all the Chinese Oides species is provided.

Four new species of Sphaeroderma Stephens (Coleoptera, Chrysomelidae, Galerucinae, Alticini) from Taiwan, with discussion on genus boundaries based on S.flavonotatum Chûjô and S.jungchani sp. nov.

Three new species of Sphaeroderma Stephens, 1831, S.hsuisp. nov., S.changisp. nov., and S.sheipaensissp. nov. are described based on specimens from Shei-Pa National Park, Taiwan. A fourth new species, S.jungchanisp. nov., is described based on specimens from southern Taiwan. Delimitation of the genus is discussed based on S.flavonotatum Chûjô, 1937, which is redescribed, and the new species, S.jungchanisp. nov.

Current knowledge on the diversity of Eumolpinae (Coleoptera, Chrysomelidae) in New Caledonia.

The Eumolpinae leaf beetles of New Caledonia are very diverse, but our knowledge about their diversity is still incomplete. Following a renewed interest in the group in the last two decades, there has been an exponential increase in the number of species described, with species descriptions and taxonomic reassessment ongoing. In this work, the catalogue of New Caledonian Eumolpinae is updated, incorporating all these recent changes, and also indicating the collection where type specimens are currently available. The updated catalogue includes 120 species in 13 genera, and more additions and taxonomic changes, including new combinations, are expected in forthcoming years. Here two new synonymies are reported, namely Dumbeastriata Jolivet, Verma & Mille, 2007 = Taophilacancellata Samuelson, 2010, syn. nov.; and Dematochromatheryi Jolivet, Verma & Mille, 2010 = Dematochromapoyensis Jolivet, Verma & Mille, 2010, syn. nov. Moreover, two species still retaining their original adscription to the genus Colaspis Fabricius, 1801, are treated as incertae sedis. This catalogue represents a useful tool for future taxonomic studies of New Caledonian Chrysomelidae and can assist biodiversity surveys and conservation studies within the archipelago.

Demarchushsui (Coleoptera, Chrysomelidae, Galerucinae, Alticini), a new species from Taiwan, with notes on immatures and biology.

A new species of the little-known genus Demarchus Jacoby was discovered at Pilu, East Taiwan, and is here described as Demarchushsuisp. nov. The larvae and adults utilise showy mistletoes as food plants. Their remarkable biology is described in detail, including egg deposition and leaf mining behaviour. Their biology is compared with that of other members of the genus.

Revision of the Palaearctic species of the genus Plateumaris C. G. Thomson, 1859 (Coleoptera, Chrysomelidae, Donaciinae).

Ten of the 27 species of Plateumaris Thomson (Chrysomelidae: Donaciinae) occur in the Palaearctic. Due to the intraspecific variation and the large distributions of some species, descriptions exist for at least 80 taxa plus five nomina nuda. The status of each valid species is clarified and the remaining 70 names are allocated as synonyms. New synonymies are P.tenuicornis Balthasar, considered a synonym of P.consimilis (Schrank), P.sulcifrons Weise as a synonym of P.rustica (Kunze), and P.caucasica Zaitzev as a synonym of P.sericea (Linnaeus). Two controversial synonyms are confirmed: P.discolor (Panzer) and P.sericeasibirica (Solsky) are both synonyms of P.sericea. Finally, P.obsoleta Jacobson is a synonym but at present it is not possible to decide whether it belongs to P.shirahatai Kimoto or to P.sericea. Forty-one new country records are added, compared with the Catalogue of Palaearctic Coleoptera published in 2010; 28 records are based on recently published records and 13 are first records for a specific country.

Original descriptions of Palaearctic species of the genus Plateumaris C. G. Thomson, 1859 (Coleoptera, Chrysomelidae, Donaciinae) and their translations.

Many original descriptions of beetles were published in Latin with specific idioms and technical terms, which are sometimes difficult to understand. The exact meaning of these descriptions is necessary for taxonomic and systematic research. Of the ten Palaearctic Plateumaris species regarded as valid three were described in English, the remaining seven in Latin, French, or German: P.amurensis Weise, 1898, P.bracata (Scopoli, 1772), P.consimilis (Schrank, 1781), P.roscida Weise, 1912, P.rustica (Kunze, 1818), P.sericea (Linnaeus, 1758), and P.weisei (Duvivier, 1885). These seven non-English original descriptions and their translations into English are presented here. Additionally, the translations of the first descriptions of the genus Plateumaris and of its 19 synonyms (some were described in Russian, also) are given.

Host Phylogeny Structures the Gut Bacterial Community Within Galerucella Leaf Beetles.

Gut microbes play important roles for their hosts. Previous studies suggest that host-microbial systems can form long-term associations over evolutionary time and the dynamic changes of the intestinal system may represent major driving forces and contribute to insect dietary diversification and speciation. Our study system includes a set of six closely related leaf beetle species (Galerucella spp.) and our study aims to separate the roles of host phylogeny and ecology in determining the gut microbial community and to identify eventual relationship between host insects and gut bacteria. We collected adult beetles from their respective host plants and quantified their microbial community using 16S rRNA sequencing. The results showed that the gut bacteria community composition was structured by host beetle phylogeny, where more or less host-specific gut bacteria interact with the different Galerucella species. For example, the endosymbiotic bacteria Wolbachia was found almost exclusively in G. nymphaea and G. sagittariae. Diversity indicators also suggested that α- and ß-diversities of gut bacteria communities varied among host beetle species. Overall, our results suggest a phylogenetically controlled co-occurrence pattern between the six closely related Galerucella beetles and their gut bacteria, indicating the potential of co-evolutionary processes occurring between hosts and their gut bacterial communities.

Can mollusks or insects serve as bioindicators of the risk element polluted area? Gastropods (Gastropoda) versus leaf beetles (Coleoptera: Chrysomelidae).

Two groups of invertebrates, terrestrial mollusks (Gastropoda) and Chrysomela populi leaf beetles (Coleoptera: Chrysomelidae), were sampled to estimate the suitability of these organisms as bioindicators of risk element pollution (predominantly Cd, Pb, and Zn) alongside the risk element concentration gradient in the contaminated area (former mining/smelting areas in the vicinity of Príbram city, Central Bohemia, Czech Republic). The individuals representing ten species of terrestrial snails and imagoes of C. populi were collected manually at five sampling sites, differing in the level of soil contamination with risk elements. The findings showed high variability of the results regardless of the element determined, animal species, and sampling location. Among the elements, higher accumulation ability was observed for Cd and Zn, given the higher bioaccessibility of these elements in soils compared to Pb, Cr, and Cu. Higher Cd and Zn accumulation in the soft tissues of gastropods (without any statistically significant differences among the species) compared to C. populi was also recorded. Medians of the bioaccumulation factors (BAFs) reached up to 33.2 for Cd and 5.8 for Zn, in gastropods while reaching up to 3.4 for Cd, and 2.3 for Zn, for C. populi. For both groups of organisms, paradoxically, a higher rate of accumulation of risk elements was observed in all analyzed organisms in sites with lower soil contamination compared to heavily contaminated sites. This indicated the ability of the organisms living in extreme conditions to avoid the uptake of these elements or to move among areas of different contamination levels. Thus, terrestrial gastropods and C. populi proved to be unsuitable bioindicators for assessing soil pollution.

Revision of Aplosonyx Chevrolat, 1836 (Coleoptera, Chrysomelidae, Galerucinae) from China, with descriptions of three new species.

In this study, 21 species of the leaf-beetle genus Aplosonyx in China are described, including three new species, Aplosonyxancorellasp. nov., Aplosonyxnigricornissp. nov. and Aplosonyxwudangensissp. nov., and 1 new record, Aplosonyxduvivieri Jacoby, 1900. Additionally, Aplosonyxancorafulvescens Chen, 1964 is elevated to species. A key to the Chinese species of Aplosonyx is provided.

Characterization of the Complete Mitochondrial Genome of a Flea Beetle Luperomorpha xanthodera (Coleoptera: Chrysomelidae: Galerucinae) and Phylogenetic Analysis.

In this study, the mitochondrial genome of Luperomorpha xanthodera was assembled and annotated, which is a circular DNA molecule including 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA genes (12S rRNA and 16S rRNA), and 1388 bp non-coding regions (A + T rich region), measuring 16,021 bp in length. The nucleotide composition of the mitochondrial genome is 41.3% adenine (A), 38.7% thymine (T), 8.4% guanine (G), and 11.6% cytosine (C). Most of the protein-coding genes presented a typical ATN start codon (ATA, ATT, ATC, ATG), except for ND1, which showed the start codon TTG. Three-quarters of the protein-coding genes showed the complete stop codon TAR (TAA, TAG), except the genes COI, COII, ND4, and ND5, which showed incomplete stop codons (T- or TA-). All the tRNA genes have the typical clover-leaf structure, except tRNASer1 (AGN), which has a missing dihydrouridine arm (DHU). The phylogenetic results determined by both maximum likelihood and Bayesian inference methods consistently supported the monophyly of the subfamily Galerucinae and revealed that the subtribe Luperina and genus Monolepta are polyphyletic groups. Meanwhile, the classification status of the genus Luperomorpha is controversial.

Bean Leaf Beetle (Ootheca spp.) (Coleoptera: Chrysomelidae) Management via Planting Timing and Insecticides.

Bean leaf beetles (Ootheca spp.) (Coleoptera: Chrysomelidae) are one of Africa's most important pests of the common bean (Phaseolus vulgaris L.). Roots, leaves, floral parts, and young pods are all attacked, leading to a considerable loss in grain yield. In Uganda, there are no comprehensive prescribed management strategies for bean leaf beetles, but farmers typically try to control the pest by delaying bean crop sowing, and to a lesser extent, using insecticides. Although farmers have consistently implemented the two approaches, there is no information on the effects of the approaches in Uganda. To assess the impact of planting timing and insecticide spray regimes on bean leaf beetle populations, concomitant foliar damage, and grain yield, we set up trials in three agro-ecological zones with known presence of the beetles during the second rainy season of 2016 (2016) and the first rainy season of 2017 (2017). The first planting, coinciding with early planting, was conducted within one week after the onset of rains. The second planting, coinciding with mid planting, followed two weeks later, while the third planting, considered late planting in this study, was conducted one month after the second planting. A foliar application of cypermethrin commencing at 7 days after emergence (DAE), 14 DAE, 21 DAE, 28 DAE, and 35 DAE; a soil drench of imidacloprid at planting combined with a foliar spray starting at 7 DAE; and an untreated control were among the insecticide spray regimes evaluated. Higher bean leaf beetle abundance was recorded from mid-planting, while higher foliar damage was recorded from late planting in two of the three agro-ecological zones. However, higher marketable grain yield was recorded from early planting in all agro-ecological zones, suggesting that delayed planting may not be beneficial. Insecticide application reduced foliar damage and increased marketable grain yield, with a combination of soil drench and foliar spray resulting in much less foliar damage and, as a result, higher grain yield. However, this did not result in economic benefits. Furthermore, marketable grain yield was higher when insecticide spray regimes were combined with early planting in all agro-ecological zones during both seasons. Our findings suggest that the common bean should be planted early and that the control of the bean leaf beetle should target both the adults and the juvenile stages in the soil. Therefore, there is a need for farmers to be able to access less-expensive soil treatments.

Revision of the genus Arthrotus Motschulsky, 1858 (Coleoptera, Chrysomelidae, Galerucinae) of Taiwan, with notes on color polymorphism.

Seven species of Arthrotus are recognized and redescribed: A.abdominalis (Chûjô, 1962), A.gressitti Kimoto, 1969, A.hirashimai Kimoto, 1969, A.fulvus Chûjô, 1938, A.saigusai Kimoto, 1969, A.tricolor (Chûjô, 1965), and A.testaceus Gressitt & Kimoto, 1963. Also, two new species are described: A.yuae sp. nov. and A.yangi sp. nov. Three new synonyms are proposed: Proegmenataiwana Takizawa, 1978 syn. nov., Dercetinanakanei Kimoto, 1969 syn. nov. and A.shibatai Kimoto, 1984 syn. nov. Lectotypes are designated for A.fulvus Chûjô, 1938 and Dercestraabdominalis Chûjô, 1962. Color polymorphism of each species is delimited base on more than 1800 specimens.

Mycorrhiza-Tree-Herbivore Interactions: Alterations in Poplar Metabolome and Volatilome.

Plants are continuously interacting with other organisms to optimize their performance in a changing environment. Mycorrhization is known to affect the plant growth and nutrient status, but it also can lead to adjusted plant defense and alter interactions with other trophic levels. Here, we studied the effect of Laccaria bicolor-mycorrhization on the poplar (Populus x canescens) metabolome and volatilome on trees with and without a poplar leaf beetle (Chrysomela populi) infestation. We analyzed the leaf and root metabolomes employing liquid chromatography-mass spectrometry, and the leaf volatilome employing headspace sorptive extraction combined with gas-chromatography-mass spectrometry. Mycorrhization caused distinct metabolic adjustments in roots, young/infested leaves and old/not directly infested leaves. Mycorrhization adjusted the lipid composition, the abundance of peptides and, especially upon herbivory, the level of various phenolic compounds. The greatest change in leaf volatile organic compound (VOC) emissions occurred four to eight days following the beetle infestation. Together, these results prove that mycorrhization affects the whole plant metabolome and may influence poplar aboveground interactions. The herbivores and the mycorrhizal fungi interact with each other indirectly through a common host plant, a result that emphasizes the importance of community approach in chemical ecology.

Two new species of the genus Smaragdina Chevrolat, 1836 (Coleoptera, Chrysomelidae, Cryptocephalinae) from China.

Two new species of the genus Smaragdina Chevrolat from China are reported: S.hejingensis Duan, Wang & Zhou sp. nov. from Xinjiang, and S.magnipunctata Duan, Wang and Zhou sp. nov. from Yunnan. Six species, S.divisa (Jacoby), S.insulana Medvedev, S.kimotoi Lopatin, S.laboissierei (Pic), S.laosensis Kimoto & Gressitt, and S.oculata Medvedev are new country records for China. Color illustrations and line drawings of general habitus and morphological details are given. All types of two new species are deposited in the collection of Institute of Zoology, Chinese Academy of Sciences (IZ-CAS).

Calothecanigromaculata species-group from sub-Saharan Africa with descriptions of two new species from KwaZulu-Natal (Chrysomelidae, Galerucinae, Alticini).

Calotheca Heyden is a flea beetle genus with a largely sub-Saharan distribution and currently comprising 34 species. The examination of new material is revealing an increase in species richness and intraspecific variability. Calothecacarolineae sp. nov. and C.wanati sp. nov., both from KwaZulu-Natal in the Republic of South Africa, are here described and attributed to the C.nigromaculata (Jacoby) species group, mainly based on genitalic characters. Photographs of the main diagnostic characters are provided, including the habitus, median lobe of the aedeagus, and spermatheca. Information on the geographic distribution and host plants of these species is also provided.

Revision of Sphenoraia Clark, 1865 (Coleoptera, Chrysomelidae, Galerucinae) from China, with descriptions of two new species.

In this study, ten species of Sphenoraia Clack, 1865 are recognized and re-described: Sphenoraia (Sphenoraioides) anjiensis Yang & Li, 1998, Sphenoraia (Sphenoraioides) berberii Jiang, 1992, Sphenoraia (Sphenoraioides) duvivieri (Laboissière, 1925), Sphenoraia (Sphenoraioides) haizhuensis Yang, 2021, Sphenoraia (Sphenoraioides) micans (Fairmaire, 1888), Sphenoraia (Sphenoraioides) nebulosa (Gyllenhal, 1808), Sphenoraia (Sphenoraioides) nigromaculata Jiang, 1992, Sphenoraia (Sphenoraioides) punctipennis Jiang, 1992, Sphenoraia (Sphenoraioides) rutilans (Hope, 1831), and Sphenoraia (Sphenoraioides) yajiangensis Jiang, 1992. Two new species, Sphenoraia (Sphenoraia) decemmaculata Feng, Yang & Liu, sp. nov. and Sphenoraia (Sphenoraioides) flavomarginata Feng, Yang & Li, sp. nov., are described. Additionally, Sphenoraia (Sphenoraia) cupreata Jacoby, 1890 and Sphenoraia (Sphenoraia) nigra Wang, Li & Yang, 2000 are transferred from Sphenoraia to Gallerucida. A key to the 12 Chinese species of Sphenoraia is given.

The genus Japonitata Strand (Insecta, Coleoptera, Chrysomelidae, Galerucinae) in Taiwan: a redefinition of the genus and descriptions of two new species.

The genus Japonitata is redefined based on comparison with its allied genera Paraplotes Laboissière, 1933 and Shairella Chûjô, 1962. Japonitataquadricostata Kimoto, 1996 and J.caerulea Kimoto, 1996 are transferred to Shairella. Japonitatahoujayi sp. nov. and J.jungchani sp. nov. are described. Biological information is provided for J.houjayi sp. nov. In addition, the generic boundary of Shairella is redefined by including S.quadricostata and S.caerulea.