[Comparison of active ingredients and protective effects of Dendrobium huoshanense of different growth years on acute liver injury].

The differences of the active ingredients in Dendrobium huoshanense of different growth years and their protective effects on acute liver injury were studied to provide evidence for optimizing harvest time. The contents of polysaccharides, total flavonoids and total alkaloids in D. huoshanense of different growth years were determined by UV spectrophotometry, and the contents of gigantol in D. huoshanense were determined by HPLC. C57 BL/6 mice were randomly divided into blank control group(saline), modeling group(saline), high-dose(7.5 g·kg~(-1)) and low-dose(1.25 g·kg~(-1)) groups of D. huoshanense of different growth years. Each group was intragastrically administered every day for 2 weeks. 500 mg·kg~(-1) paracetamol was injected intraperitoneally 2 h after last treatment except the control group. After 12 hours, the serum and liver tissues were collected to detect the activities of ALT and AST, and the levels of SOD and MDA. The hepatic histopathological examination was performed. The results showed that the chemical constituents of D. huo-shanense of different growth years were significantly different(P<0.05). The contents of polysaccharide and gigantol of D. huoshanense of 2 growth years were the highest. The contents of flavonoids and alkaloids of D. huoshanense of 3 growth years were the hig-hest, followed by the D. huoshanense of 2 growth years, and the lowest were that of 1 growth year. Compared with the modeling group, D. huoshanense of different growth years could decrease the activities of ALT and AST in serum. Meanwhile, the levels of MDA reduced significantly, while those of SOD increased markedly. Histopathological results suggested that all D. huoshanense samples were effective in the reduction of the necrosis of hepatocytes in different degrees. The results of the multi-component SPSS paired tests showed that polysaccharide and gigantol probably played a leading role in the liver protection effects, while D. huoshanense of 2 growth years showed the best efficacy. The optimal harvesting time of D. huoshanense is 2 growth years.

Comparison of active ingredients and protective effects of Dendrobium huoshanense of different growth years on acute liver injury / 中国中药杂志

The differences of the active ingredients in Dendrobium huoshanense of different growth years and their protective effects on acute liver injury were studied to provide evidence for optimizing harvest time. The contents of polysaccharides, total flavonoids and total alkaloids in D. huoshanense of different growth years were determined by UV spectrophotometry, and the contents of gigantol in D. huoshanense were determined by HPLC. C57 BL/6 mice were randomly divided into blank control group(saline), modeling group(saline), high-dose(7.5 g·kg~(-1)) and low-dose(1.25 g·kg~(-1)) groups of D. huoshanense of different growth years. Each group was intragastrically administered every day for 2 weeks. 500 mg·kg~(-1) paracetamol was injected intraperitoneally 2 h after last treatment except the control group. After 12 hours, the serum and liver tissues were collected to detect the activities of ALT and AST, and the levels of SOD and MDA. The hepatic histopathological examination was performed. The results showed that the chemical constituents of D. huo-shanense of different growth years were significantly different(P<0.05). The contents of polysaccharide and gigantol of D. huoshanense of 2 growth years were the highest. The contents of flavonoids and alkaloids of D. huoshanense of 3 growth years were the hig-hest, followed by the D. huoshanense of 2 growth years, and the lowest were that of 1 growth year. Compared with the modeling group, D. huoshanense of different growth years could decrease the activities of ALT and AST in serum. Meanwhile, the levels of MDA reduced significantly, while those of SOD increased markedly. Histopathological results suggested that all D. huoshanense samples were effective in the reduction of the necrosis of hepatocytes in different degrees. The results of the multi-component SPSS paired tests showed that polysaccharide and gigantol probably played a leading role in the liver protection effects, while D. huoshanense of 2 growth years showed the best efficacy. The optimal harvesting time of D. huoshanense is 2 growth years.

Protective effect and nursing intervention of magnesium magnesium oxalate on liver function after hepatectomy / 中国生化药物杂志

Objective To study the protective effect of nursing intervention and magnesium oxalate on liver function after hepatectomy. Methods 64 cases of liver resection patients in our hospital were randomly divided into the control group and the experimental group, with 32 patients in each group. In the control group, patients were given 150mg magnesium magnesium sulfate for 7 days after the operation. On this basis treatment of the control group, the experimental group was carried out targeted nursing intervention, payed attention to the patient's psychological state, patiently explain to the patients, did well preoperative preparation, and closely observed the correlation of liver function. Clinical indicators of the two groups of patients were compared and analyzed. Results 7 days after operation, the level of ALT in the experimental group was (50.20±23.21) U/L, and the level of AST was (25.40±9.03) U/L. The level of ALT in the control group was (95.43±43.90) U/L, and the level of AST was (38.33 ± 9.19) U/L, there were statistical significance in two groups (P<0.05). There were no obvious adverse reactions in two groups, and there was no statistical significance (P<0.05). The level of serum ALT in the experimental group decreased to normal range, and the number of patients was significantly higher than that of the control group with statistical significance (P<0.05). Conclusion The effect of Magnesium Oxalate and nursing intervention on liver function after hepatectomy is ideal. It can significantly improve the liver function of patients with high safety and clinical significance.

Protective effect and nursing intervention of magnesium magnesium oxalate on liver function after hepatectomy / 中国生化药物杂志

Objective To study the protective effect of nursing intervention and magnesium oxalate on liver function after hepatectomy. Methods 64 cases of liver resection patients in our hospital were randomly divided into the control group and the experimental group, with 32 patients in each group. In the control group, patients were given 150mg magnesium magnesium sulfate for 7 days after the operation. On this basis treatment of the control group, the experimental group was carried out targeted nursing intervention, payed attention to the patient's psychological state, patiently explain to the patients, did well preoperative preparation, and closely observed the correlation of liver function. Clinical indicators of the two groups of patients were compared and analyzed. Results 7 days after operation, the level of ALT in the experimental group was (50.20±23.21) U/L, and the level of AST was (25.40±9.03) U/L. The level of ALT in the control group was (95.43±43.90) U/L, and the level of AST was (38.33 ± 9.19) U/L, there were statistical significance in two groups (P<0.05). There were no obvious adverse reactions in two groups, and there was no statistical significance (P<0.05). The level of serum ALT in the experimental group decreased to normal range, and the number of patients was significantly higher than that of the control group with statistical significance (P<0.05). Conclusion The effect of Magnesium Oxalate and nursing intervention on liver function after hepatectomy is ideal. It can significantly improve the liver function of patients with high safety and clinical significance.

Antioxidant, Liver Protective and Angiotensin I-converting Enzyme Inhibitory Activities of Old Laying Hen Hydrolysate in Crab Meat Analogue.

The purpose of this study was to evaluate the antioxidative activities of Crab meat analogue prepared with protein hydrolysates obtained from mechanically deboned chicken meat (MDCM) from spent laying hens. 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) radical-scavenging activity was increased by adding MDCM hydrolysates during storage, and activity correlated with the concentration of DPPH added up to 6 weeks of storage. Hydroxyl radical-scavenging activity was increased in all analogues containing MDCM hydrolysates. At 0 days of storage, angiotensin I-converting enzyme (ACE)-inhibitory activity was increased by the addition of MDCM hydrolysates. Activity did not correlate after 6 weeks of storage, in which ACE-inhibitory activity was increased with low concentrations of MDCM hydrolysates, but no ACE-inhibitory activity was observed at higher concentrations. The liver-protecting activity of crab meat analogue was shown to be around 60% of the positive control; however, it was not significantly different among the samples during storage. These results support the use of MDCM as a source of health-promoting constituents in crab meat analogue.