Changes in the Lipid Metabolism of the Longissimus thoracis Muscle in Bulls When Using Different Feeding Strategies during the Growing and Finishing Phases.

The objective was to evaluate the supplementation strategy's effect on beef cattle during the growing phase and two systems during the finishing phase. One hundred and twenty young bulls were randomly divided in a 2 × 2 factorial design to receive either mineral (ad libitum) or protein + energy (3 g/kg body weight (BW)/day) during the growing phase and pasture plus concentrate supplementation (20 g/kg BW/day) or feedlot (25:75% corn silage:concentrate) during the finishing phase. Feedlot-fed bulls had meat (Longissimus thoracis-LT) with a higher content of lipids and saturated and monounsaturated fatty acids and a greater upregulation of stearoyl-CoA desaturase and sterol regulatory element-binding protein-1c than animals that fed on pasture (p < 0.05). On the other hand, pasture-fed bulls had meat with a higher content of α-linoleic acid, linolenic acid, and n6 and a greater n6:n3 ratio compared to the feedlot-fed group (p < 0.05). In addition, meat from pasture-fed bulls during the finishing phase had 17.6% more isocitrate dehydrogenase enzyme concentration than the feedlot group (p = 0.02). Mineral-fed and pasture-finished bulls showed down-regulation of peroxisome proliferator-activated receptor gamma (p < 0.05), while the bulls fed protein + energy and finished in the feedlot had higher carnitine palmitoyltransferase 2 expression (p ≤ 0.013). In conclusion, mineral or protein + energy supplementation in the growing does not affect the fatty acid composition of intramuscular fat of LT muscle. In the finishing phase, feeding bulls in the feedlot upregulates the lipogenic genes and consequently improves the intramuscular fat content in the meat.

Evaluation of different percentages of Duroc genes and gender on growth, carcass and meat quality traits for pigs.

The effect of different percentages of Duroc genes in crossbreeding (5, 50 and 100%), the gender (immunocastrated males - ICM and females) and their interaction was evaluated on growth, carcass and meat quality traits for pigs. Crossbreds (50% Duroc) had greater average daily feed intake, hot carcass weight and backfat thickness but lower meat yield when compared to 5% Duroc crossbreds and purebred (100% Duroc) animals (P < 0.05). Purebred (100% Duroc) animals had the lowest backfat thickness and subcutaneous fat thickness, and the greatest muscle depth and meat yield (P < 0.05). ICM animals had better feed conversion, greater average daily gain, hot carcass weight and amount of meat, and lower hot carcass yield, cold carcass weight, Longissimus thoracis depth, rib with belly and ham weights compared to females (P < 0.05). Marbling scores were greater in purebred (100%) animals (P < 0.05). The meat from 50% Duroc crossbreds and 100% Duroc purebred pigs was more reddish pink in color than 5% Duroc crossbreds (P < 0.05). Also, marbling scores were greater for females compared to ICM (P < 0.05). Purebred (100% Duroc) animals required more medications during production (P < 0.05).

Polimorfismos en genes candidatos a la composición de ácidos grasos y su efecto en carne Wagyu-Cross

RESUMEN Objetivo. Comparar la composición de ácidos grasos (FA) en la carne Wagyu y sus cruzas con Angus, Beefmaster, Brangus y Hereford, y analizar su relación con marcadores genéticos del metabolismo lipídico. Materiales y métodos. Se colectaron 111 muestras de Longissimus dorsi, las cuales se agruparon por grupo genético, se cuantificaron los FA (cromatografía de gases líquidos) y se tipificaron marcadores de ADN asociados teóricamente a FA. Se examinó el equilibrio de Hardy-Weinberg y desequilibrio de ligamiento de los marcadores y se estimó el efecto de las cruzas y el efecto de los genotipos. Resultados. Las cruzas no mostraron diferencias substanciales en la composición de FA con respecto a Wagyu. Nueve SNPs mostraron asociación con la composición de FA, y se encontró un efecto importante en el marcador SLC2A4 ss62538460, el cual influyó sobre SFA, MUFA y MUFA/SFA. Los marcadores, PLTP ss77832104 y IGF2R ss77831885 influyeron sobre C16:0, MYOZ1 ss77832104 sobre C17:1 y PPARGC1A c.1892+19 sobre C18:2. Además, se comprobaron efectos previamente descritos de MEF2C ss38329156 y SCD c.878. Conclusiones. Los presentes resultados representan una de las primeras evidencias sobre la deposición de FA en ganado Wagyu y sus cruzas y propone algunos loci en genes candidatos con posibilidad de implementación en estrategias de mejoramiento asistido.

ABSTRACT Objective. To assess the fatty acids composition (FA) in Wagyu beef and its crosses with Angus, Beefmaster, Brangus and Hereford, and to analyze its relationship with genetic markers related to lipid metabolism. Materials and methods. 111 Longissimus dorsi samples were collected and grouped by genetic group. FA were extracted and quantified in a gas-liquid chromatography and DNA markers theoretically associated to FA were typed. Hardy-Weinberg equilibrium and linkage disequilibrium were examined, the effect of crosses and the effect of genotypes were estimated. Results. The crosses did not show substantial differences in FA composition. Nine SNPs showed association with FA composition, and a significant effect was found in the SLC2A4 marker ss62538460 which influenced SFA, MUFA and MUFA/SFA; PLTP ss77832104 and IGF2R ss77831885 markers, influenced C16:0, MYOZ1 ss77832104 on C17:1 and PPARGC1A c.1892+19 on C18:2. In addition, previously described effects of MEF2C ss38329156 and SCD c.878 were supported. Conclusions. These results are first evidence on FA deposition in Wagyu cattle and their crosses, and proposes some loci in candidate genes with the possibility of implementation in assisted selection strategies.

Nutrient supplementation of beef female calves at pre-weaning enhances the commitment of fibro-adipogenic progenitor cells to preadipocytes.

We aimed to evaluate the impact of nutrient supplementation of beef female calves at pre-weaning on adipogenic determination. Thirty-four female calves were assigned to two experimental treatments: Control (CON, n = 17), where animals were supplemented only with mineral mixture; Supplemented (SUP, n = 17), where animals received energy-protein supplement containing minerals (5 g/kg of BW per day) of their body weight. Animals were supplemented from 100 to 250 days of age, and muscle samples were biopsied at the end of the supplementation period. Regarding the performance variables, there were no differences between treatments for initial body weight (P = 0.75). The final body weight (P = 0.07), average daily gain (P = 0.07), rib eye area (P = 0.03), and rib fat thickness (P = 0.08) were greater in SUP female calves compared with CON treatment. The number of fibro-adipogenic progenitor cells (P = 0.69) did not differ between treatments, while a greater number of intramuscular pre-adipocytes were observed in SUP than CON female calves (P = 0.01). The expression of miRNA-4429 (P = 0.20) did not differ between treatments, while the expression of miRNA-129-5p (P = 0.09) and miRNA-129-2-3p (P = 0.05) was greater in CON than SUP female calves. Our results suggest that nutrient supplementation at early postnatal stages of development enhances the commitment of fibro-adipogenic progenitor cells into the adipogenic lineages allowing to an increase in intramuscular fat deposition potential of the animals later in life.

Effect of Cow-Calf Supplementation on Gene Expression, Processes, and Pathways Related to Adipogenesis and Lipogenesis in Longissimus thoracis Muscle of F1 Angus × Nellore Cattle at Weaning.

The aim of this study was to identify differentially expressed genes, biological processes, and metabolic pathways related to adipogenesis and lipogenesis in calves receiving different diets during the cow-calf phase. Forty-eight uncastrated F1 Angus × Nellore males were randomly assigned to two treatments from thirty days of age to weaning: no creep feeding (G1) or creep feeding (G2). The creep feed offered contained ground corn (44.8%), soybean meal (40.4%), and mineral core (14.8%), with 22% crude protein and 65% total digestible nutrients in dry matter. After weaning, the animals were feedlot finished for 180 days and fed a single diet containing 12.6% forage and 87.4% corn-based concentrate. Longissimus thoracis muscle samples were collected by biopsy at weaning for transcriptome analysis and at slaughter for the measurement of intramuscular fat content (IMF) and marbling score (MS). Animals of G2 had 17.2% and 14.0% higher IMF and MS, respectively (p < 0.05). We identified 947 differentially expressed genes (log2 fold change 0.5, FDR 5%); of these, 504 were upregulated and 443 were downregulated in G2. Part of the genes upregulated in G2 were related to PPAR signaling (PPARA, SLC27A1, FABP3, and DBI), unsaturated fatty acid synthesis (FADS1, FADS2, SCD, and SCD5), and fatty acid metabolism (FASN, FADS1, FADS2, SCD, and SCD5). Regarding biological processes, the genes upregulated in G2 were related to cholesterol biosynthesis (EBP, CYP51A1, DHCR24, and LSS), unsaturated fatty acid biosynthesis (FADS2, SCD, SCD5, and FADS1), and insulin sensitivity (INSIG1 and LPIN2). Cow-calf supplementation G2 positively affected energy metabolism and lipid biosynthesis, and thus favored the deposition of marbling fat during the postweaning period, which was shown here in an unprecedented way, by analyzing the transcriptome, genes, pathways, and enriched processes due to the use of creep feeding.

Effect of the gain rate during growing phase on the performance and characteristics of carcass and meat of super precocious steers.

The aim was to evaluate the effect of different rates of weight gain during the backgrounding on animal performance and carcass and meat characteristics of steers finished in feedlots. Thirty-six Angus steers, 12 ± 2 months of age, were backgrounded during 91 days on Aruana grass pasture (Panicum maximum cv. Aruana) managed under different stocking rates to achieve three different weight gains: HIGH ADG (average daily gain)-no feed restriction (ADG = 0.846 kg); MEDIUM ADG-moderate feed restriction (ADG = 0.456 kg); and LOW ADG-high feed restriction (ADG = 0.154 kg). To ensure the difference in ADG, we offered 0.7% live weight of concentrate feed in the HIGH treatment and a better forage supply in the MEDIUM treatment. After the backgrounding, the animals were finished in feedlot. There was no effect of the previous performance on the animals' performance in the feedlot. The LOW presented higher weight (218.9 vs. 207.9 kg) and hot (54.0% vs. 51.3%) and cold (53.5% vs. 50.5%) carcass yield than the MEDIUM, besides presenting meat with less cooking losses (15.0% vs. 18.2%), marbling (7 vs. 4.0 points), palatability (7.1 vs. 6.3 points), juiciness (7.2 vs. 6.4 points), tenderness (7.1 vs. 6.3 points), and lower shear force (5.78 vs. 8.75 kg) than HIGH. LOW ADG steers stay longer in the finishing phase but presented in general better quality carcass and meat than those with MEDIUM or HIGH during the backgrounding.

Effect of vitamin A injection at birth on intramuscular fat development and meat quality in beef cattle.

This study aimed to evaluate intramuscular fat and expression of genes in the muscle of Montana × Nellore treated with vitamin A at birth. We hypothesized that an injection of vitamin A after birth would increase marbling by increasing the expression of angiogenic, adipogenic, and lipogenic genes. Animals treated with vitamin A had greater marbling in the longissimus muscle (P = 0.05). The vitamin A treatment increased the expression of VEGFA gene at 40 days of age and at weaning and increased the expression of ZNF423 at weaning and at harvesting (P ≤ 0.03). The expression of WNT was higher (P = 0.01) at 40 days of age and at weaning in the animals treated with vitamin A. Vitamin A also increased the expression of SREBF1 at 40 days of age and at weaning (P ≤ 0.05). Therefore, the administration of vitamin A to cattle at birth could be a way to increase carcass marbling without affecting the performance of the animals.

Metabolome fingerprints, performance and carcass quality of beef calves supplemented with antibiotic free additive.

The aim of this study was to investigate the effects of probiotics on pre-weaning performance, muscle, and fat deposition and serum metabolite profiles in male and female Senepol calves. Thirty new-born Senepol calves, 15 males and 15 females, were randomly allocated to the following treatments: CON a control group that received the basal creep feeding diet and PRO animals that received the basal diet with addition of 2 g/100 kg of body weight (BW) of probiotic. PRO supplementation did not change the DMI but increased average BW, final BW, ADG relative to animals fed CON. Additionally, PRO improved LMA and marbling. Regardless of the serum metabolite profile, the important metabolites for discriminating PRO and CON were glutamine, leucine, creatine, acetate, creatinine, arginine, glutamate, hippurate, glycerol, carnitine, lactate, carnosine, myo-inositol and histidine. According to gender, males had an overabundance of glutamine, glycerol, isoleucine, creatinine and glucose, whereas females had an overabundance of acetyl carnitine, glutamate and carnitine. In conclusion, the addition of PRO in the pre-weaning diet of calves increases performance, weight at weaning and muscle and fat deposition on the carcass, improving proteins and fatty acid metabolism, the immune system response and rumen development.

Effect of growth and carcass traits on likelihood of early pregnancy in Nellore heifers raised at intensive nutritional plan

This study evaluated the influence of the quantitative traits measured by real-time ultrasound (adjusted longissimus muscle area [AdjLMA], adjusted rump fat thickness [AdjRFT], and adjusted marbling [AdjMAR]) as well as age at first breeding [AFB] and adjusted weight [AdjWeight], on the probability of occurrence of early pregnancy (EP) in 55 Nellore heifers, and also performed an economic analysis. All calves received supplementation in creep-feeding (ad libitum), and at weaning (average age= 210 ± 30 days; average weight= 241 ± 33 kg) until first breeding by artificial insemination (May to November) all heifers were managed in the same group (two paddocks of 25 ha each evenly covered with Urochloa Marandu Grass) and received protein-energy supplementation (1% of average BW per animal/day). The quantitative variables were collected immediately after timed artificial insemination (average age= 392 ± 27 days; average weight= 431.90 kg), and the pregnancy diagnosis was completed at 30 days following insemination. For economic analysis, two systems were compared (age at first breeding at 13 and 24 months). The greater adjusted weight on the first breeding increased the probability of occurrence of early pregnancy, while the greater adjusted longissimus muscle area reduced. In addition, intensive meat production systems provide greater economic return throughout cow-calf operation.(AU)

Color and Marbling as Predictors of Meat Quality Perception of Argentinian Consumers.

In Argentina, color and intramuscular fat are the main attributes of raw beef quality; however, it is necessary to clarify how consumers use them, in order to establish different marketing strategies. Consumer preferences are affected by multiple factors. Thus, the objective of the present study was to identify the Argentinian consumer's choice behavior regarding beef color and fat content. An online survey was carried out in Argentina. It inquired about socio-demographic characteristics, purchase and consumption habits and beliefs, showing pictures related to color, marbling and the amount of fat. Choice behavior was evaluated by asking why consumers chose a particular picture out of the ones shown. Several Kruskal-Wallis tests evaluated the different hypotheses. Three different decision trees using the CHAID analysis method were created. Multifactorial analysis was carried out for clustering consumers. Regarding consumer beliefs, 90% of the respondents agreed with the sentence, "The two main characteristics defining beef quality at purchase time are meat color and marbling". Socio-demographic characteristics affected purchase habits and beliefs; they also affected perceptions about meat color and marbling. It was possible to build three consumer groups for future marketing strategies: "hedonic" focused on a pleasing sensory experience, "appearance" prioritized the visual aspects, and the "health-conscious" consumers were interested in their healthy nutrition.

Random-effect meta-analysis of genetic parameter estimates for carcass and meat quality traits in beef cattle.

Considerable variability of genetic parameter estimates is observed among different studies for the same trait, which is associated with the distinct effects included in the statistical model, population breed, and sample sizes. The random-effect meta-analysis summarizes genetic parameters considering the heterogeneity among studies. Therefore, the aim of this study was to perform a random-effect meta-analysis of heritability and genetic correlation estimates for carcass and meat quality traits in beef cattle. A total of 152 estimates of heritability and 83 genetic correlations for longissimus muscle area (LMA), back fat thickness (BFT), and marbling score (MRB) were used. High heterogeneity among published studies was observed for all traits, indicating the need of a random-effects model to perform the analysis. Estimates of heritability through the meta-analysis using the random-effects model were high (0.30 to 0.34), indicating that fast genetic progress can be obtained for these traits. However, genetic correlations had low magnitude (lower than 0.25), which suggested that all three traits should be included in the selection scheme.

Effects of the association between whole cottonseed and calcium salts of fatty acids on nutrient intake, feedlot performance, and carcass characteristics of Bos indicus animals offered a high-concentrate diet.

This experiment evaluated the effects of feeding whole cottonseed (WC) and/or calcium salts of fatty acids (CSFA) on dry matter intake (DMI), performance, and carcass characteristics of Bos indicus animals receiving a high-concentrate diet during the finishing phase. On day 0, 96 Nellore bulls were blocked according to initial shrunk body weight (BW; 302 ± 26.7 kg) into group pens (four animals/pen) and, within blocks, pens were randomly assigned to receive: 1) 15% of WC and 2% of CSFA (dry matter [DM] basis) of palm, cottonseed, and soybean oil (15WC; n = 6); 2) 10% of WC and 3% of CSFA (DM basis) of palm, cottonseed, and soybean oil (10WC; n = 6); 3) 5% of WC and 4% of CSFA (DM basis) of palm, cottonseed, and soybean oil (5WC; n = 6); and 4) 0% of WC and 5% of CSFA (DM basis) of palm, cottonseed, and soybean oil (0WC; n = 6). Diets were formulated to be isocaloric, isonitrogenous, and isolipidic. Experimental period lasted 108 d; DMI was evaluated daily, whereas blood samples and carcass measurements were obtained on days 0, 55, and 108 of the study. Upon slaughter on day 109, steaks were collected for determination of the chemical and fatty acid (FA) profile of the meat. No treatment effects (P ≥ 0.35) were observed on DMI, performance, average daily gain (ADG), carcass ultrasound measurements, and chemical variables of the steak. Nonetheless, including WC into the diets decreased C12:0, C16:0, C16:1 trans-9, C17:0, C18:0, C18:1 cis-9, C18:2 cis-9, cis-12, C18:3 cis-9, cis-12, cis-15, saturated, and unsaturated FA intake (P < 0.01). Moreover, adding WC increased DMI fluctuation and feed efficiency (FE; P = 0.03) but decreased marbling (P ≤ 0.03). A treatment × day interaction was observed (P < 0.01) for serum leptin concentration, as 10WC animals had greater leptin concentration on day 103 vs. other treatments (P < 0.01). Regarding steak FA profile, WC addition into the diet increased C18:2 cis-7, trans-9 and C18:3 cis-9, cis-12, cis-15 (P < 0.001), whereas saturated FA was quadratically affected (P = 0.02) and unsaturated FA was reduced for 15WC (P < 0.04). In summary, increasing levels of CSFA into isolipidic finishing diets containing WC did not negatively impact feedlot performance but reduced FE and increased marbling scores of B. indicus bulls, demonstrating its feasibility as a technology to improve carcass traits of low-marbling animals.

Perception of beef quality for Spanish and Brazilian consumers.

Understanding the priorities of beef consumers is important for planning strategies to attract and maintain each customer. Using self-administered interviews with Spanish and Brazilian adults who regularly consume beef, we analyzed their purchasing behaviors in relation to their perceptions and priorities regarding beef quality that guide their choices. The current study identifies the influences of information (labeling and marketing) requirements, concerns about humane production systems, and the intrinsic characteristics of beef. To evaluate the data extracted from the interviews, the questions were grouped into four main factors. While extrinsic factors are the highest valued in both Spain and Brazil, those such as beef freshness and tenderness present inverse concerns between the two countries based on cultural dissimilarities. The data also reveals a clear symmetry among consumers who increasingly seek specific pre-purchase information and products with guaranteed superior quality. Despite other characteristics that differentiate developed and developing countries, the perceptions of quality among consumers in Spain and Brazil are decidedly similar.

Immunocastration on performance and meat quality of Bos indicus (Nellore) cattle under different nutritional systems

The present study aimed to evaluate the effects of immunocastration on the animal growth, carcass traits, and meat quality of Nellore cattle receiving or not Ionophore supplementation at pasture or pasture with concentrate (semi-feedlot). The first experiment (Pasture) was carried out during the rainy season, while the second (supplemented = Semi-feedlot) was conducted during the dry season. In each assessment, 60 males were allocated into three treatments, in a completely randomized design: non-castrated, immunocastrated (anti-gonadotropin-releasing hormone vaccine), and immunocastrated animals receiving ionophore. The highly energetic supplemented diet was prepared using 85 % corn and 10 % soybean meal at 1 % body weight, while the ionophore was provided with salt containing 1,650 mg kg−1 of lasalocid. After slaughter, the carcasses were graded, and Longissimus thoracis et lumborum samples were collected for meat quality analysis. The non-castrated animals exhibited a more significant final body weight and hot carcass weight than those immunocastrated regardless of ionophore supplementation (p < 0.05). The carcasses of non-castrated animals had less fat cover and marbling than castrated animals. A high incidence of dark cutting beef was verified in non-castrated animals. The immunocastrated cattle produced tenderer beef, mainly at pasture rearing. In conclusion, regardless of production system or ionophore supplementation, immunocastration was an adequate choice to generate high-quality meat, since it increased marbling and improved beef tenderness. However, limitations regarding animal growth should be estimated.(AU)

Meat quality of cattle subjected to period of aging process: a cross-heifer study / Qualidade da carne de bovinos submetida ao processo de maturação: estudo com novilhas cruzadas

The aim of this study was to evaluate the effects of aging on meat quality of crossbred heifers. Qualitative and quantitative evaluations of carcasses and meat of 148 crossbred heifers with an average age of 13 months and 407.5 ± 29.22 kg body weight at slaughter were performed. After cooling for 24 h, the carcasses were evaluated for hot carcass weight, pH, conformation, physiological maturity, finishing, fat distribution, and length. Characteristics, such as pH, color, marbling, exudation, as well as cooking losses, percentage of ether extract, and shear force were evaluated in meat at time zero and after seven days of aging. The carcasses displayed satisfactory results for parameters such as pH at 24 h of cooling (pH24), finishing, distribution of fat, and subcutaneous fat thickness (5.58, 3.27, 2.40, and 6.62 mm, respectively). The meat had a low marbling score (5.08) and percentage of ether extract (1.71), indicating that the meat was relatively lean. Exudation and cooking losses showed similar results at zero and seven days of aging. The meat subjected to seven days of aging showed higher lightness and hue (40.98 and 0.77, respectively) and tenderness of 5.25 kg. These results indicated that aging affected meat color and shear force parameters; the meat of cross-heifers was softer but less red after maturation.(AU)

O objetivo deste estudo foi avaliar os efeitos da maturação na qualidade da carne de novilhas mestiças. Foram realizadas avalações quali-quantitativas de carcaças e carne de 148 novilhas mestiças com idade média de 13 meses e 407,5 ± 29,22 kg de peso corporal ao abate. Após resfriamento por 24 horas, as carcaças foram avaliadas quanto ao peso da carcaça quente, pH, conformação, maturidade fisiológica, acabamento, distribuição de gordura e comprimento. Características como pH, cor, marmoreio, perdas por exsudação, assim como perdas por cocção, porcentagem de extrato etéreo e força de cisalhamento foram avaliadas na carne no tempo zero e após sete dias de maturação. As carcaças apresentaram resultados satisfatórios para parâmetros com pH em 24 horas de resfriamento (pH24), acabamento, distribuição de gordura e espessura de gordura subcutânea (5,58, 3,27, 2,40 e 6,62 mm, respectivamente). A carne apresentou baixo índice de marmoreio (5,08) e porcentagem de extrato etéreo (1,71), indicando que a carne era relativamente magra. As perdas por exsudação e cozimento apresentaram resultados semelhantes aos zero e sete dias de maturação. A carne submetida a sete dias de maturação apresentou maior luminosidade e hue (40,98 e 0,77, respectivamente) e maciez de 5,25 kg. Esses resultados indicaram que a maturação afetou os parâmetros de cor da carne e a força de cisalhamento; a carne das novilhas cruzadas ficou mais macia, mas menos vermelha após a maturação.(AU)

Immunocastration on performance and meat quality of Bos indicus (Nellore) cattle under different nutritional systems

The present study aimed to evaluate the effects of immunocastration on the animal growth, carcass traits, and meat quality of Nellore cattle receiving or not Ionophore supplementation at pasture or pasture with concentrate (semi-feedlot). The first experiment (Pasture) was carried out during the rainy season, while the second (supplemented = Semi-feedlot) was conducted during the dry season. In each assessment, 60 males were allocated into three treatments, in a completely randomized design: non-castrated, immunocastrated (anti-gonadotropin-releasing hormone vaccine), and immunocastrated animals receiving ionophore. The highly energetic supplemented diet was prepared using 85 % corn and 10 % soybean meal at 1 % body weight, while the ionophore was provided with salt containing 1,650 mg kg−1 of lasalocid. After slaughter, the carcasses were graded, and Longissimus thoracis et lumborum samples were collected for meat quality analysis. The non-castrated animals exhibited a more significant final body weight and hot carcass weight than those immunocastrated regardless of ionophore supplementation (p < 0.05). The carcasses of non-castrated animals had less fat cover and marbling than castrated animals. A high incidence of dark cutting beef was verified in non-castrated animals. The immunocastrated cattle produced tenderer beef, mainly at pasture rearing. In conclusion, regardless of production system or ionophore supplementation, immunocastration was an adequate choice to generate high-quality meat, since it increased marbling and improved beef tenderness. However, limitations regarding animal growth should be estimated.

Likelihood of obtaining tender meat from confined calf / Probabilidade de maciez da carne de novilhos confinados

The objective was to evaluate the main factors that influence the shear strength of meat from confined steers and the probability of obtaining soft meat. For this purpose, we evaluated the literature on carcass and/or meat of beef steers in Brazil published between January 1999 and April 2019 and extracted the content from three sections for analysis: materials and methods, results, and discussion. Pearsons correlation was used to analyze the data, and the stepwise statistic was used to determine the proportion of the synchronized effect of variables on shear force. For determining the probability of tenderness, meat with a shear force lower than 4.6 kgf/cm3 was classified as soft; meat with a higher sheer force was classified as hard. Following the classification, logistic regression analysis and odds ratio test were performed. The factors of study location, the proportion of zebu background in the genome, finishing weight, the percentage of concentrate in the diet, and finishing period and meat marbling explained 62.45% of the variability in the shear strength of beef. The following strategies were found to increase the chances of effectively obtaining soft meat from confined steers: starting the termination phase early even in animals with lower weights, prolonging the confinement time, increasing concentrate percentage in the diet, and a higher marbling degree. It is possible to estimate a large proportion of shear force variability using the production variables (ante-mortem), and the process can be adjusted accordingly to considerable increase the possibility of obtaining soft meat.(AU)

Objetivou-se avaliar os principais fatores que influenciam a força de cisalhamento da carne de novilhos confinados e a chance de obtenção de carne macia. Para isso, foram avaliados os artigos de trabalhos científicos realizados no Brasil sobre carcaça e/ou carne de novilhos de corte, publicados entre janeiro de 1999 a abril de 2019. Foram extraídas da literatura informações do material e método e resultados e dos discussão. Para análise dos dados foi utilizado correlação de Pearson, e a estatística stepwise para a determinação da proporção do efeito sincronizado das variáveis sobre a força de cisalhamento. Para a determinação da chance de maciez, as carnes foram classificadas como macia quando apresentavam força de cisalhamento inferior a 4,6 kgF/ cm3 e dura quando superior a 4,6 kgf/ cm3, seguido de análise de regressão logística e teste de ods ratio. O local em que o estudo foi desenvolvido, o percentual de zebuíno no genótipo, o peso inicial da terminação, o percentual de concentrado na dieta, o período de terminação e marmoreio da carne, explicaram 62,45% da variabilidade da força de cisalhamento da carne. Iniciar precocemente a fase de terminação, mesmo que com pesos mais leves; prolongar o tempo de confinamento; elevar a participação de concentrado na dieta e aumentar o grau de marmoreio da carne; são estratégias que efetivamente aumentam a chance de obtenção de carne macia de novilhos confinados. A partir de variáveis de produção (ante-mortem), pode-se estimar grande parte da variabilidade da força de cisalhamento, sendo que sua correta manipulação possibilitará incrementos significativos na chance de obtenção de carne macia.(AU)

Effect of corn processing on growth performance, carcass characteristics, and plasma glucose-dependent insulinotropic polypeptide and metabolite concentrations in feedlot cattle.

The objectives of this trial were to evaluate the association between corn processing, glucose-dependent insulinotropic polypeptide (GIP) concentration, and intramuscular (IM) fat deposition. We hypothesized that steers fed whole shelled corn (WSC) would have a greater IM fat deposition than steers fed cracked corn (CC) due to an increase in plasma GIP concentration. Backgrounded, Angus-cross cattle (initial body weight [BW] = 279 ± 9.8 kg) were used in a randomized complete block design in a feedlot setting for an average of 230 d. Cattle were allotted in 12 pens (6 pens per treatment with 8 animals per pen). There were three blocks: heifers (n = 32, initial BW = 265 ± 1.3 kg), small steers (n = 32, initial BW = 262 ± 1.3 kg), and large steers (n = 32, initial BW = 310 ± 1.4 kg). Two pens within each block were randomly assigned to one of the following treatments: 1) CC or 2) WSC. Animal growth performance, carcass characteristics, and plasma hormone and metabolite concentrations were analyzed using the MIXED procedure of SAS, including the fixed effects of treatment, or treatment, time, and their interaction. Pen and block were included as random effects. Carcass yield and quality grade distributions were compared using the GLIMMIX procedure of SAS. including the fixed effects of treatment and time with pen and block as random effects. Linear regression was used to evaluate the association of plasma GIP concentration and IM fat content. Average daily gain (P = 0.57) and final BW (P = 0.34) were similar, regardless of treatment. Cattle fed CC had reduced (P < 0.01) dry matter intake (DMI) when compared with those fed WSC. This lesser DMI resulted in improved gain:feed ratio (P < 0.01) for cattle fed CC compared with cattle fed WSC. There was no effect (P ≤ 0.33) of corn processing on plasma glucose, plasma GIP concentrations, hot carcass weight, dressing percentage, or marbling score. There was a positive linear relationship (P = 0.03) between IM fat concentration and plasma GIP concentration. In conclusion, feeding CC increased gain:feed ratio compared with WSC, but there was no difference in plasma GIP concentration, whereas plasma GIP concentration appears to be related to IM fat deposition.

Beef quality of Nellore steers fed dried or rehydrated and ensiled corn or sorghum grains / Calidad de la carne de Ganado Nelore alimentado con grano de maíz o sorgo seco o rehidratado y ensilado / Qualidade de carne de bovinos Nelore alimentados com grãos de milho ou sorgo seco ou reidratado e ensilado

Abstract Background: Rehydration of grains, such as corn and sorghum, is used to increase nutrient absorption. However, the effect of this practice on meat quality is poorly understood. Objective: To evaluate the effects of type of grain and processing on the meat quality of Nellore steers in a feedlot. Methods: Twenty-four non-castrated Nellore steers (270 ± 53 kg initial body weight) were distributed in a completely randomized 2×2 factorial design, with six replicates. The first factor was cereal type (corn or sorghum), and the second was the grain processing (dry or rehydrated and ensiled). The diets were composed of 30% corn silage and 70% concentrate. Sixty days before the beginning of the experiment, corn and sorghum grains were rehydrated and ensiled. The animals were slaughtered after 140 days of confinement. Meat quality analyses were determined in samples of fresh and aged meat (7 days) from the Longissimus lumborum muscle. Results: no difference between treatments was observed for carcass pH and L* (lightness), a* (redness), and b* (yellowness) values, shear force, thawing and cooking losses, and chemical composition of meat (p>0.05). The b* (yellowness) value of subcutaneous fat was higher in steers fed corn, regardless of grain processing (p=0.03). Sarcomere length was higher in aged meat of steers fed sorghum, regardless of processing method (p=0.01). Conclusions: the grain processing method does not affect beef quality; however, grain type can affect subcutaneous fat color and sarcomere length of aged beef.

Resumen Antecedentes: La rehidratación de granos, tales como maíz y sorgo, se usa para aprovechar mejor sus nutrientes. Sin embargo, es poco conocido el efecto de esta práctica sobre la calidad de la carne. Objetivo: Evaluar los efectos del tipo de cereal y su procesamiento sobre la calidad de carne de toretes Nelore en confinamiento. Métodos: Veinticuatro novillos Nelore enteros con un peso promedio inicial de 270 ± 53 kg, se distribuyeron en un diseño factorial 2×2 completamente aleatorizado (n=6). El primer factor fue el tipo de cereal (maíz o sorgo) y el segundo fue su procesamiento (seco o rehidratado y ensilado). Las dietas estuvieron compuestas por 28,44% de ensilaje de maíz y 71,56% de concentrado. Sesenta días antes de iniciar el experimento, los granos de maíz y sorgo se rehidrataron y ensilaron. Los animales se sacrificaron después de 140 días de confinamiento. La calidad de la carne se determinó en muestras de carne fresca y madurada (7 días) obtenidas del músculo Longissimus lumborum. Resultados: No hubo diferencias entre tratamientos para el pH de las canales, ni para los valores de color (L* "luminosidad", a* "intensidad de rojo" y b* "intensidad de amarillo"), fuerza de corte, composición química, o pérdidas de agua por descongelamiento o cocción (p>0,05). El valor de b* de la grasa subcutánea fue mayor en los novillos alimentados con maíz, independientemente del tipo de procesamiento (p=0,03). La longitud de sarcómero fue más alto en la carne madurada de novillos alimentados con sorgo, sin importar el método de procesamiento (p=0,01). Conclusiones: El método de procesamiento del grano no afecta la calidad de la carne; sin embargo, el tipo de cereal afecta el color de la grasa subcutánea y la longitud del sarcómero de la carne madurada.

Resumo Antecedentes: A reidratação de grãos, como milho e sorgo, tem sido usada para aumentar a utilização de seus nutrientes. Contudo, o efeito dessa prática na qualidade da carne é pouco compreendido. Objetivo: Avaliar os efeitos do tipo de grão de cereais e seu processamento sobre a qualidade da carne de bovinos confinados. Métodos: Vinte e quatro bovinos Nelore não castrados, com peso corporal médio inicial de 270 ± 53 kg foram distribuídos em delineamento inteiramente casualizado, fatorial 2×2, com seis repetições. O primeiro fator foi o tipo de grão de cereal no concentrado (milho ou sorgo), e o segundo foi o processamento destes grãos (seco ou reidratado e ensilado). As dietas foram compostas por 28,44% de silagem de milho e 71,56% de concentrado. Sessenta dias antes do início do experimento, os grãos de milho e sorgo foram reidratados e ensilados. Os animais foram abatidos após 140 dias de confinamento. As análises de qualidade da carne foram determinadas em amostras não maturadas e maturadas (7 dias) obtidas do músculo Longissimus lumborum. Resultados: Não houve diferenças significativas (p>0,05) entre os tratamentos para pH da carcaça e L* (luminosidade), a* (intensidade de vermelho), b* (intensidade de amarelo), força de cisalhamento, perdas por descongelamento, perdas por cocção, perdas totais e composição química da carne. O valor de b* da gordura subcutânea foi maior (p=0,03) em bovinos alimentados com dietas contendo grãos de milho, independentemente do tipo de processamento. O comprimento de sarcômero foi maior na carne maturada de novilhos alimentados com sorgo, independente do método de processamento (p=0,01). Conclusões: O método de processamento dos grãos não afetou a qualidade da carne bovina, no entanto, a cor da gordura subcutânea e o comprimento de sarcômero da carne maturada foram afetados pelo tipo de grão.

Proteomic analysis reveals changes in energy metabolism of skeletal muscle in beef cattle supplemented with vitamin A.

BACKGROUND: Vitamin A has been reported as a factor influencing marbling deposition in meat from animals. Although the mechanisms by which vitamin A regulates lipid metabolism in mature adipocytes are already well-established, information regarding molecular mechanisms underlying the effects of vitamin A on the regulation of intramuscular fat deposition in beef cattle still remains limited. The present study aimed to assess the molecular mechanisms involved in the intramuscular fat deposition in beef cattle supplemented with vitamin A during the fattening phase using a proteomic approach. RESULTS: Vitamin A supplementation during the fattening phase decreased intramuscular fat deposition in beef cattle. Proteome and phospho-proteome analysis together with biological and networking analysis of the protein differentially abundant between treatments indicated that Vitamin A supplementation affects the overall energy metabolism of skeletal muscle, impairing lipid biosynthesis in skeletal muscle. CONCLUSION: Vitamin A supplementation at fattening phase impairs intramuscular fat deposition in beef cattle likely by changing the energy metabolism of skeletal muscle. The interaction of retinoic acid and heat shock 70-kDa protein may play a pivotal role in intramuscular fat deposition as a consequence of vitamin A supplementation by impairing de novo fatty acid synthesis as a result of a possible decrease in insulin sensitivity in the skeletal muscle. © 2020 Society of Chemical Industry.