Methylation Modification in Ornamental Plants: Impact on Floral Aroma and Color.

Methylation represents a crucial class of modification that orchestrates a spectrum of regulatory roles in plants, impacting ornamental characteristics, growth, development, and responses to abiotic stress. The establishment and maintenance of methylation involve the coordinated actions of multiple regulatory factors. Methyltransferases play a pivotal role by specifically recognizing and methylating targeted sites, which induces alterations in chromatin structure and gene expression, subsequently influencing the release of volatile aromatic substances and the accumulation of pigments in plant petals. In this paper, we review the regulatory mechanisms of methylation modification reactions and their effects on the changes in aromatic substances and pigments in plant petals. We also explore the potential of methylation modifications to unravel the regulatory mechanisms underlying aroma and color in plant petals. This aims to further elucidate the synthesis, metabolism, and regulatory mechanisms of various methylation modifications related to the aroma and color substances in plant petals, thereby providing a theoretical reference for improving the aroma and color of plant petals.

Biological effects of Bougainvillea glabra, Delonix regia, Lantana camara, and Platycladus orientalis extracts and their possible metabolomics therapeutics against the West Nile virus vector, Culex pipiens (Diptera: Culicidae).

Plants are a treasure trove of biological materials containing a wide range of potential phytochemicals that are target-specific, rapidly biodegradable, and environmentally friendly, with multiple medicinal effects. Unfortunately, the development of resistance to synthetic pesticides and antibiotics led to the discovery of new antibiotics, antioxidants, and biopesticides. This has also led to the creation of new medications that work very well. The current study aimed to prove that ornamental plants contain specialized active substances that are used in several biological processes. Mosquitoes, one of the deadliest animals on the planet, cause millions of fatalities each year by transmitting several human illnesses. Phytochemicals are possible biological agents for controlling pests that are harmful. The potential of leaf extracts of Bougainvillea glabra, Delonix regia, Lantana camara, and Platycladus orientalis against Culex pipiens and microbial agents was evaluated. Acetone extracts had more toxic effects against Cx. pipiens larvae (99.0-100%, 72 h post-treatment), and the LC50 values were 142.8, 189.5, 95.4, and 71.1 ppm for B. glabra, D. regia, L. camara, and P. orientalis, respectively. Plant extracts tested in this study showed high insecticidal, antimicrobial, and antioxidant potential. GC-MS and HPLC analyses showed a higher number of terpenes, flavonoids, and phenolic compounds. The ADME analysis of element, caryophyllene oxide, caryophyllene, and copaene showed that they were similar to drugs and that they were better absorbed by the body and able to pass through the blood-brain barrier. Our results confirm the ability of ornamental plants to have promising larvicidal and antimicrobial activity and biotechnology.

Propagule pressure from historic U.S. plant sales explains establishment but not invasion.

Introduction history, including propagule pressure and residence time, has been proposed as a primary driver of biological invasions. However, it is unclear whether introduction history increases the likelihood that a species will be invasive or only the likelihood that it will be established. Using a dataset of non-native species historically available as ornamental plants in the conterminous United States, we investigated how introduction history relates to these stages of invasion. Introduction history was highly significant and a strong predictor of establishment, but only marginally significant and a poor predictor of invasive success. Propagule pressure predicted establishment better than residence time, with species likely to be established if they were introduced to only eight locations. These findings suggest that ongoing plant introductions will lead to widespread establishment but may not directly increase invasive success. Instead, other characteristics, like plant traits and local scale processes, may better predict whether a species becomes invasive.

Effect of monocultures and polycultures of Typha latifolia and Heliconia psittacorum on the treatment of river waters contaminated with landfill leachate/domestic wastewater in partially saturated vertical constructed wetlands.

Partially Saturated Vertical Constructed Wetlands (PSV-CWs) are novel wastewater treatment systems that work through aerobic and anaerobic conditions that favor the removal of pollutants found in high concentrations, such as rivers contaminated with domestic wastewater and landfill leachate. The objective of the study was to evaluate the efficiency of PSV-CWs using monocultures and polycultures of Typha latifolia and Heliconia psittacorum to treat river waters contaminated with leachates from open dumps and domestic wastewater. Six experimental units of PSV-CWs were used; two were planted with Typha latifolia monoculture, two with Heliconia psittacorum monoculture and two with polycultures of both plants. The results indicated better organic matter and nitrogen removal efficiencies (p < 0.05) in systems with polycultures (TSS:95%, BOD5:83%, COD:89%, TN:82% and NH4+:99%). In general, the whole system showed high average removal efficiencies (TSS:93%, BOD5:79%, COD:85%, TN:79%, NH4+:98% and TP:85%). Regarding vegetation, both species developed better in units with monocultures, being Typha latifolia the one that reached a more remarkable development. However, both species showed high resistance to the contaminated environment. These results showed higher removals than those reported in the literature with conventional Free Flow Vertical Constructed Wetlands (FFV-CWs), so PSV-CWs could be a suitable option to treat this type of effluent.

The research addresses the contamination of water resources in developing countries by landfill leachate and domestic wastewater discharges. It proposes treatment through Partially Saturated Vertical Constructed Wetlands (PSV-CWs), which, despite the limited information available, have been shown to be effective in removing pollutants in effluents with high concentrations. In addition to evaluating PSV-CWs, the study examines the impact of different types of vegetation on pollutant removal efficiency, concluding that PSV-CWs are a promising and viable option for the treatment of these effluents.

Influence of Bed Depth on the Development of Tropical Ornamental Plants in Subsurface Flow Treatment Wetlands for Municipal Wastewater Treatment: A Pilot-Scale Case.

The aim of this 2-year study was to evaluate the influence of bed depth (40 and 60 cm) on the development of tropical ornamental species (Alpinia purpurata, Heliconia latispatha and Strelitzia reginae) and on the removal of different contaminants such as chemical oxygen demand (COD), nitrate (N-NO3), ammonium (N-NH4), total nitrogen (TN), total phosphorus (TP), total suspended solids (TSS), total coliforms (TCs) and fecal coliforms (FCs), in horizontal subsurface flow constructed wetlands (HSSF-CWs) for municipal wastewater treatment. The results showed that the depth of 60 cm favored the removal of COD, with removal efficiencies of 94% for the three plant species. The depth of 40 cm was most effective for the removal of N-NH4 (80-90%). Regarding the removal of TN, the removals were similar for the different plants and depths (72-86%). The systems only achieved up to 60% removal of TCs and FCs. The depth of the CWs substrate and its saturation level influenced the development of ornamental vegetation, particularly flower production. For Heliconia latispatha, a bed depth level of 60 cm was more suitable, while for Alpinia purpurata 40 cm was better, and for Strelitzia reginae in both cases there was no flower production. The impact of bed depth on contaminant removal depends on the specific type of contaminant.

Treatment of real laundry wastewater using vertical flow constructed wetland planted with the ornamental climbing plant Trachelospermum jasminoides: assessing the removal of conventional pollutants and benzotriazoles.

This study investigates the effectiveness of vertical flow constructed wetlands (VFCWs) planted with a climbing ornamental plant for on-site treatment of real laundry wastewater. Specifically, the presence or absence of Trachelospermum jasminoides was evaluated for the removal performance of conventional pollutants (turbidity, TSS, COD, TP) and benzotriazoles (BTRs): 1H-benzotriazole (BTR), 5-methyl-1H-benzotriazole (5-TTR), 5-chlorobenzotriazole (CBTR), and xylytriazole (XTR). Results revealed that high removal efficiencies ranging from 92 to 98% were presented in both planted and unplanted systems for turbidity, TSS, and COD. Moreover, high removal rates were observed for CBTR and XTR, which were the only compounds found in real laundry wastewater, in both VFCW systems (planted: 100%; 94%; unplanted: 87%; 92%, respectively). The contribution of plants to the pollutant's removal was not statistically significant for all examined parameters. However, T. jasminoides demonstrated the ability to survive and grow without any visible symptoms under the harsh conditions of laundry wastewater, enabling the development of green facade. According to the findings, the application of VFCWs for on-site laundry wastewater treatment in buildings seems to be a highly promising solution, not only for primarily removing conventional pollutants but also for addressing emerging contaminants, specifically BTRs.

Assessment of the Cu phytoremediation potential of Chrysanthemum indicum L. and Tagetes erecta L. using analysis of growth and physiological characteristics.

In the current study, the Cu phytoremediation ability of two ornamental plants, Chrysanthemum indicum L. and Tagetes erecta L., was tracked concerning the growth and physiological responses. Plants were subjected to varying concentrations of Cu (0, 100, 200, and 400 mg/kg) under the pot experiment for 8 weeks. The results showed that the measured growth and physiological characteristics declined in T. erecta shoots and roots at all tested treatments compared with the control. However, in C. indicum at 100 mg/kg, shoot biomass, shoot total soluble protein, and leaves number remained equal to that of the control and then reduced by rising Cu concentrations, compared with the control. Also, results indicated that in C. indicum, after 56 days of exposure to Cu, the chlorophyll pigments content markedly increased and reached a maximum level at 100 mg/kg dose and gradually declined with enhancing Cu concentrations, compared with the control. Other measured growth and physiological parameters decreased in both tissues of C. indicum in response to Cu usage in the growth medium. The carotenoid content of T. erecta decreased in all studied Cu levels in comparison to the control, but in C. indicum remained unaffected up to 200 mg/kg Cu in comparison to the control and then enhanced with increasing Cu level. The augmentation of antioxidant enzyme activity in two species, especially in roots, reflected the incident of Cu stress as demonstrated by elevated MDA and ion leakage levels. Data concerning copper accumulation in tissues, TF, and BAF showed T. erecta is a weak Cu accumulator and seems not to be an appropriate candidate for Cu phytoremediation. However, the Cu content in shoots and roots of C. indicum increased significantly with an increment in applied Cu level. Also, C. indicum accumulated higher Cu concentrations in the roots than in shoots and exhibited TF < 1, 0.1 < BAF root < 1, and can be considered as a Cu excluder by the phytostabilization mechanism.

Endophytic Fungi Residing within Cornus florida L. in Mid-Tennessee: Phylogenetic Diversity, Enzymatic Properties, and Potential Role in Plant Health.

Endophytic fungi that reside internally in healthy, asymptomatic plants often benefit their hosts by promoting plant growth and/or providing plant protection against abiotic and biotic stresses. However, only a small fraction of the estimated 1.5 million fungal endophytes have been identified. In this study, a total of 369 isolates of fungal endophytes in 59 distinct taxa were isolated from stem samples of Cornus florida (flowering dogwood). All isolates belonged to species of phyla Ascomycota and Basidiomycota distributed across five orders and 11 genera. Isolates belonging to the same family clustered together in a phylogenetic tree generated from a cluster analysis using MEGA 7 software. Diversity indices of the fungi revealed a rich and diverse community that included several species associated with leaf spots, blight, cankers, and/or dieback diseases. Pathogenicity tests confirmed 16 fungal endophytes as C. florida pathogens, including some well-known destructive pathogens Botryosphaera dothidea, Colletotrichum acutatum, and C. gleosporoides. Isolates of the fungal endophytes possess the capacity to produce extracellular hydrolytic enzymes (cellulase, amylase, pectinase, laccase, chitinase, and protease) that are known to function in tissue penetration, plant colonization, nutrient acquisition, and disease suppression in both plant pathogens and endophytes These results support the interchangeable pathogenic-endophytic roles for some taxa.

IPPRAS Cryobank for the Conservation of Orthodox Seeds of Rare, Endangered, Medicinal, and Ornamental Plant Species-Current Research.

Plant cryobanks play a significant role in modern science and breeding. They contribute to the recovery of lost species, the emergence of new plant varieties, and help preserve and explore the diversity of the plant world. The IPPRAS Cryobank collection is constantly supplemented with new samples, while, at the same time, the stored samples are being monitored. In order to test seed germination, seeds of Allium and Veratrum species were thawed. Rare Allium species seeds, such as A. nutans, A. schoenoprasum, and A. victorialis were stored in liquid nitrogen for 17, 19, and 30 years, respectively. Long-term cryopreservation decreased germination rates for A. nutans from 96.55 to 50.00%, for A. schoenoprasum from 72.00 to 62.75%, and for A. victorialis from 90.00 to 83.05%. Seeds of a rare medicinal species, Veratrum lobelianum, were stored in liquid nitrogen for 18 years; the seed germination rate during this storage period has been significantly decreased from 75.00 to 14.81%. V. nigrum seeds were also collected and frozen in liquid nitrogen for 3 days. Short-term cryopreservation did not result in a statistically significant change in germination rates (from 79.71 to 82.69%). The seeds of an endangered ornamental species, Cypripedium calceolus, were collected and kept frozen for 3 days. After cryopreservation, the seeds were planted on three different media, as follows: ½ MS, MS with 10% coconut milk, and BM1. On ½ MS medium, 24.98% seeds formed protocorms, while on MS medium with 10% coconut milk, this number was 10.02%, and on BM1 medium, it was 15.02%, respectively; however, after 2.5 months, all of the protocorms died. Thus, it appears that the existing protocol for seed cryopreservation of C. calceolus needs further improvement. The size, weight, and free water content (WC) of six previously cryopreserved Stipa species and three Allium species were measured. For all the Allium and Stipa species studied, we found no correlation between seed size, WC, and cryotolerance. We also found no correlation between the life form, which reflects the water requirement of the species, and cryotolerance.

In Vitro Shoot Multiplication and Rooting of 'Kashan' and 'Hervy Azerbaijan' Damask Rose (Rosa damascena Mill.) Genotypes for Cosmetic and Ornamental Applications.

The damask rose (Rosa damascena Mill.) is an ornamental-medicinal plant from the Rosaceae family, and its aromatic compounds and essential oils are applied globally in the food, cosmetic, and pharmaceutical industries. Due to its economic value, this research aimed to establish a protocol for an efficient, rapid, and cost-effective method for in vitro shoot multiplication and rooting of the R. damascena 'Kashan' and 'Hervy Azerbaijan' genotypes. Nodal segments (as primary explants) were cultured on the Murashige and Skoog (MS) medium with combinations of various plant growth regulators (PGRs) such as gibberellic acid (GA3), 6-benzylaminopurine (BAP), and indole-3-butyric acid (IBA), as well as a PGR-like substance, phloroglucinol (PG), vitamins such as ascorbic acid (AA), and activated carbon in the form of active charcoal (AC). For the establishment stage, 0.1 mg·L-1 PG, 0.2 mg·L-1 GA3, and 1 mg·L-1 BAP were added to the media. Secondary explants (nodal segments containing axillary buds produced from primary explants) were obtained after 30 days of in vitro culture and transferred to the proliferation media supplemented with different concentrations of BAP (0, 0.5, 1, 1.5, 2, and 2.5 mg·L-1) and GA3 (0, 0.1, 0.2, 0.4, 0.8, and 1 mg·L-1) together with 0.1 mg·L-1 PG and 20 mg·L-1 of AA. The rooting media were augmented with different concentrations of BAP and GA3 with 0.1 mg·L-1 of IBA, PG and 20 mg·L-1 of AA and AC. The results showed that the highest regeneration coefficient (4.29 and 4.28) and the largest number of leaves (23.33-24.33) were obtained in the explants grown on the medium supplemented with 2 mg·L-1 BAP and 0.4 mg·L-1 GA3 for the 'Kashan' and 'Hervy Azerbaijan' genotypes, respectively. Likewise, this PGR combination provided the shortest time until bud break (approximately 6.5 days) and root emergence (approximately 10 days) in both genotypes. The highest number of shoots (4.78 per explant) and roots (3.96) was achieved in this medium in the 'Kashan' rose. Stem and root lengths, as well as stem and root fresh and dry weights, were also analyzed. In most measured traits, the lowest values were found in the PGRs-free control medium. Rooted plantlets were transferred to pots filled with perlite and peat moss in a 2:1 proportion and were acclimatized to ambient greenhouse conditions with a mean 90.12% survival rate. This research contributes significantly to our understanding of Damask rose propagation and has practical implications for the cosmetic and ornamental plant industries. By offering insights into the manipulation of regeneration processes, our study opens up new possibilities for the effective production of high-quality plant material.

Main Poisonous and Allergenic Plant Species in Sicilian Gardens and Parks: Applications and Recommendations for Use.

This study identified the most common poisonous and allergenic plants occurring in Sicilian gardens and parks. Based on a survey conducted at 100 sites, a list was drawn up that reports the main biological and toxicological characteristics and ornamental uses of these plants. A total of 137 taxa were recorded, of which 108 were poisonous and 32 were allergenic. The most represented families were the Solanaceae, Moraceae, Apocynaceae and Fabaceae. The most represented geographical contingents were the European and the Mediterranean. A large number of toxic and allergenic plants recorded in Sicilian parks and gardens cause gastrointestinal disorders, 21 of which are deadly poisonous. Based on the results, actions for the management of existing gardens and the construction of new ones are discussed. The importance of environmental education for the population starting from school age is stressed. These recommendations aim to preserve cultivated biodiversity and, at the same time, protect human and pet health.

Microbial perspective on restoration of degraded urban soil using ornamental plants.

The urban soil where abandoned buildings are demolished is barren and structurally poor, and this degraded soil requires restoration. Ornamental plants enhance the urban environment, increase biodiversity, and affect soil physicochemical properties, microbial diversity; however, their effects remain unclear. Thus, in this study, a mixed-planting meadow consisting of 14 perennial ornamental flower species, including Iris tectorum, Iris lacteal, and Patrinia scabiosaefolia, etc. Was planted at a demolition site with sewage-contaminated soil in Beijing. Simultaneously, a single-planting lawn of I. tectorum was established in a nearby park. We aimed to examine soil physicochemical properties, sequence soil bacterial 16S rRNA and fungal ITS amplicons, and analyze soil microbial diversity and community structure at both sites at five time points in the year after planting, To explore the effect of herbaceous ornamental plants on degraded urban soil, we used FAPROTAX and FUNGuild to predict bacterial and fungal functions, the bin-based null model to evaluate the soil microbial community, and random matrix theory to construct soil microbial molecular networks. The mixed-planting meadow produced a visually appealing landscape and dynamic seasonal enrichment, significantly increasing soil total nitrogen (TN) and organic matter (SOM) contents by 1.99 and 1.21 times, respectively. TN had a positive correlation with soil microbial α diversity and community structure. Dominant phyla at both sites included Proteobacteria, Actinobacteria, and Ascomycota. Although soil microorganisms were primarily influenced by stochastic processes, stochasticity was notably higher in the mixed-planting meadow than in the single-planting lawn. The mixed-planting meadow significantly increased the relative abundance of beneficial microorganisms, improving nitrification and aerobic ammonium oxidation of soil bacteria, as well as symbiotroph of fungi. No significant changes were observed in the single-planting lawn. The mixed-planting meadow established a complex soil microbial molecular network, enhancing the correlation between bacteria and fungi and increasing the number of key microorganisms. Our findings suggest the potential of mixed-planting meadow in restoring degraded urban soils by influencing the soil microbial community and enhancing the ecological service function. Our study provides theoretical support for applying mixed-planting meadow communities to improve the soil environment of urban green spaces.

Discovery and Characterization of Two Highly Divergent Variants of a Novel Potyvirus Species Infecting Madagascar Periwinkle (Catharanthus roseus).

During the summer of 2022, a cluster of Madagascar periwinkle plants with white and mauve flowers were observed with foliar mild yellow mosaic symptoms on a private property in Harlingen, Cameron County, Texas. The symptoms were reproduced on mechanically inoculated periwinkle and Nicotiana benthamiana plants. Virions of 776 to 849 nm in length and 11.7 to 14.8 nm in width were observed in transmission electron microscopy of leaf dip preparations made from symptomatic periwinkle leaves. High-throughput sequencing (HTS) analysis of total RNA extracts from symptomatic leaves revealed the occurrence of two highly divergent variants of a novel Potyvirus species as the only virus-like sequences present in the sample. The complete genomes of both variants were independently amplified via reverse transcriptase PCR, cloned, and Sanger sequenced. The 5' and 3' of the genomes were acquired using random amplification of cDNA ends methodology. The assembled virus genomes were 9,936 and 9,944 nucleotides (nt) long, and they shared 99.9 to 100% identities with the respective HTS-derived genomes. Each genome encoded hypothetical polyprotein of 3,171 amino acids (aa) (362.6 kilodaltons [kDa]) and 3,173 aa (362.7 kDa), respectively, and they shared 77.3/84.4% nt/aa polyprotein identities, indicating that they represent highly divergent variants of the same Potyvirus species. Both genomes also shared below-species-threshold polyprotein identity levels with the most closely phylogenetically related known potyviruses, thus indicating that they belong to a novel species. The name periwinkle mild yellow mosaic virus (PwMYMV) is given to the potyvirus with complete genomes of 9,936 nt for variant 1 (PwMYMV-1) and 9,944 nt for variant 2 (PwMYMV-2). We propose that PwMYMV be assigned into the genus Potyvirus (family Potyviridae).

A feasibility study on indoor therapeutic horticulture to alleviate sleep and anxiety problems: The impact of plants and activity choice on its therapeutic effect.

INTRODUCTION: Therapeutic horticulture (TH) is increasingly being applied for sub-health or patient mental health care. Whether plant and activity type will affect TH's effectiveness is unclear. AIM: To evaluate the feasibility of an indoor TH for alleviating the anxiety symptoms and sleeping problems of people with poor sleep quality, and explore the potential affection of plant and activity type on health benefits. METHOD: Thirty subjects (all with sleep problems and half with anxiety problems) were randomly assigned to three groups to do horticultural activities with ornamental plants, general aromatic plants, or aromatic plants with reported mental health functions, respectively. Six indoor TH activities were then held sequentially within two weeks. Psychological scales, subjective feedback questionnaires, and physiological indicators were used as evaluation indexes before and after horticulture activities. RESULTS: The TH relieved subjects' sleep and anxiety problems and was particularly effective in alleviating anxiety among people with high anxiety levels. Using ornamental plants was more effective in relieving stress while functional aromatic plants performed better in sleep improvement and satisfaction to TH. Each horticultural activity could improve mood state but showed different effects on the vitality of the participants. CONCLUSION: The above findings provided some basis for the potential benefits of selecting plants and activities based on psychological care needs in the development of TH plans. Future research that expands upon the current project is warranted. A larger sample size is beneficial for obtaining more powerful statistical results.

Nitrogen and spent coffee ground for enhancing nutritional, morphological, flowering and antioxidant properties of Chrysanthemum (Chrysanthemum morfolium Ramat).

Chrysanthemum is one of the most attractive flowering plants widely grown commercially worldwide. Having a good source of organic fertilizers plays an important role in meeting the increasing demand for these plants, which requires high-quality flowers and a high survival time for the longest period. The effect of nitrogen (N) coupled with spent coffee ground (SCG) at various levels (0.0, 2.5, 5.0, 7.5, 10.0°% w/w) was evaluated on growth performance and chemical components of the Chrysanthemum over two years in a pot scale. Overall, total dry matter (TDM) was significantly enhanced with N+ by 125 and 97°% over N- in the first and second years, respectively. SCG also enhanced TDM up to the highest level of application in the range of 27-98°% and 18-81°% over SCG (0.0°%) in the same years, respectively. The interaction effect between N and SCG was perfect on TDM, flower number, and flower dry weight. Similarly, total antioxidant activities when N and SCG were coupled together gave respective increments ranging from 11.8 to 45.9 U/g DW and from 2.1 to 15.9 U/g DW compared to N alone (5.8 and 0.9 U/g DW) in both leaves and flowers, respectively. Extracts of plant treated with N and 10°% SCG exhibited a higher content of rosmarinic, caffeic, chlorogenic, vanillic acids, and rutin in the leaves. SCG as a natural organic source is easy to obtain and is a practical and cost-effective solution to plant nutrition, which can be valuable for ornamental plants, especially when combined with nitrogen.

Side chain of confined xylan affects cellulose integrity leading to bending stem with reduced mechanical strength in ornamental plants.

The stem support for fresh-cut flowers exerts a profound influence on the display of their blossoms. During vase insertion, bending stems significantly affect the ornamental value, but much remains unclear about the underlying reasons. In this study, six pairs of ornamental plants were screened for the contrast of bending and straight stems. The bending stems have weakened mechanical force and biomass recalcitrance compared with the straight ones. Meanwhile, cells in the bending stems became more loosely packed, along with a decrease in cell wall thickness and cellulose levels. Furthermore, wall properties characterizations show bending stems have decreased lignocellulosic CrI and cellulose DP, and enhanced the branching ratio of hemicellulose which is trapped in the cellulose. Given the distinct cell wall factors in different species, all data are grouped in standardized to eliminate the variations among plant species. The principal composition analysis and correlation analysis of the processed dataset strongly suggest that cellulose association factors determine the stem mechanical force and recalcitrance. Based on our results, we propose a model for how branches of confined hemicellulose interacted with cellulose to modulate stem strength support for the straight or bending phenotype in cut flowers.

Genetic Engineering and Genome Editing Advances to Enhance Floral Attributes in Ornamental Plants: An Update.

The ornamental horticulture industry is a highly dynamic and rapidly changing market. Constant development of novel cultivars with elite traits is essential to sustain competitiveness. Conventional breeding has been used to develop cultivars, which is often laborious. Biotechnological strategies such as genetic engineering have been crucial in manipulating and improving various beneficial traits that are technically not possible through cross-breeding. One such trait is the highly desired blue-colored flower in roses and chrysanthemums, which can be achieved through transgenic technology. Advances in genome sequencing platforms have enhanced the opportunities to access the whole genome sequence in various ornamentals, facilitating the dissection of the molecular genetics and regulatory controls of different traits. The recent advent of genome editing tools, including CRISPR/Cas9, has revolutionized plant breeding. CRISPR/Cas9-based gene editing offers efficient and highly precise trait modification, contributing to various beneficial advancements. Although genome editing in ornamentals is currently in its infancy, the recent increase in the availability of ornamental genome sequences provides a platform to extend the frontiers of future genome editing in ornamentals. Hence, this review depicts the implication of various commercially valuable ornamental attributes, and details the research attempts and achievements in enhancing floral attributes using genetic engineering and genome editing in ornamental plants.

Ornamental Plants and Urban Gardening.

Urban green areas serve both the mental and physical health of the people living in the settlements; therefore, the ornamental plants used on green areas currently have a prominent role in reducing the effects of climate change and urbanization, as well as in providing ecosystem services. This is a dynamically changing, new field that requires close cooperation with several scientific fields, such as landscape architecture and plant physiology, genetics, plant breeding, and ecology. The monitoring and research of settlement communities as ecological systems greatly serves the perception of the effects of climate change and helps to mitigate them. The sustainability and economic operation of established urban green space systems can be made effective by applying innovative technologies. The Special Issue "Ornamental Plants and Urban Gardening" was launched in 2022 and published 13 articles on the topic until 31 July 2023. The published articles also have a very wide spectrum of topics, which also shows the diversity and the interdisciplinary nature of the scientific field. In the following, we present the main topics of the published articles and the results with which their authors contributed to the enrichment of the scientific field. We present a brief summary of the articles in shorter subsections.

Field evaluation of organic and synthetic insecticides against the hala scale, Thysanococcus pandani (Hemiptera: Halimococcidae), on Maui, Hawaii.

The hala scale, Thysanococcus pandani Stickney, is an invasive insect that infests hala trees, Pandanus tectorius, and has recently arrived in the Hawaiian Islands of Maui, Oahu, and Molokai. Hala scale is native to Thailand, Indonesia, and Singapore and was accidentally introduced to Maui, with its first detection in Hana in 1995. The hala tree is a frequently planted ornamental plant throughout Hawaii's urban landscape and is also a native tree found in coastal areas of ecological suitability throughout the state. These trees are integral to native Hawaiian weaving traditions, as the leaves were used to make items such as sails, hats, or mats. The spread of T. pandani threatens the continuation of these practices, as the plant material may not be sufficient for weaving. Hala trees infested with T. pandani can have negative effects such as deformation, discoloration, and early tree death. Experimental field trials were set up to evaluate the efficacy of organic and chemical insecticides as potential treatments to mitigate the damage of T. pandani infestations. This was done on the Island of Maui, where T. pandani is widespread. These trees were artificially inoculated with T. pandani and evaluated throughout the trials for changes in plant height, canopy width, scale removal, and visual infestation ratings. The results of 2 repeated field trials indicated that flupyradifurone and buprofezin were effective treatments in the reduction of the hala scale infestation and improving visual aspects related to plant health.