Long-Term Effect of Crop Succession Systems on Soil Chemical and Physical Attributes and Soybean Yield.

Most soybean producers in the Cerrado biome use the direct seeding system, making it essential to cultivate cash or cover crops in the off-season, to promote soil protection, as well as increase organic matter, which is directly related to improvements in the chemical and physical characteristics of these soils. In this sense, this work was conducted in Jataí, state of Goias, Brazil, to evaluate the physical-chemical attributes of the soil and the performance of soybeans cultivated in different crop succession systems cultivated for 6 years in the region of Jataí, GO. The experimental design was randomized blocks with four plots and four replications; the crops that followed soybeans were arranged as follows: T1-corn (Zea mays); T2-pearl millet (Pennisetum glaucum); T3-Urochloa ruziziensis; and T4-corn + Urochloa ruziziensis. Soybean yield components and grain yield were evaluated in two harvests (2020/2021 and 2021/2022). Deformed and undisturbed soil samples were collected in 2022 to assess soil fertility and for physical analysis. The data were subjected to analysis of variance (F test) and the means were compared using the Tukey test at 5% probability. The soybean-millet succession system stood out for the chemical and physical attributes of the soil: calcium, magnesium, base saturation, hydrogen + aluminum, and total porosity. The crop succession system did not affect yield for the two years analyzed, but the accumulated grain yields were higher in the crop succession soybean/corn intercropped. The results highlight the importance of using cover crops in improving the physical and chemical qualities of the soil in the long term. However, in the Cerrado, there is a predominance of the soybean/corn succession system motivated by financial issues to the detriment of the qualitative aspects of the soil, in which the introduction of Urochloa ruziziensis in intercropping with corn would improve the chemical attributes of the soil and have a long-term impact on the accumulated grain production.

Is pearl millet (Pennisetum glaucum) a good plant species for ecotoxicological tests?

Various plant species can be selected for environmental testing, including pearl millet (Pennisetum glaucum (L.) R. Br), a globally significant cereal crop. This study aims to assess millet's suitability as a species for ecotoxicological tests, examining (1) germination and initial development dynamics, (2) the minimum seed quantity for reliable sampling, (3) optimal experimental design with replication numbers, (4) suitability of positive control, and (5) the effectiveness of the protocol in evaluating toxic effects of environmental pollutants. Millet exhibited rapid and uniform germination as well as consistent initial seedling development. To establish the minimum number of seeds required for reliable experimentation, germination, and seedling growth were compared across plots containing 10, 25, and 50 seeds. Consequently, 10 seeds per plot were chosen for subsequent experiments to reduce labor and costs while maintaining reliability. To validate the selected experimental design, and to establish a positive control for assays, aluminum was used as a toxic element at concentrations ranging from 10-2 to 10-6 M. While aluminum did not affect the final percentage of germinated seeds, it did exhibit an impact on the Germination Speed Index (GSI). Significant differences in root and aerial growth, and with fresh weight, were observed. The 10-3M concentration was chosen as the positive control as the 10-2 concentration showed extreme toxicity. To assess the applicability of the established protocol in determining the toxic effects of environmental pollutants, millet roots were exposed to the toxic agents atrazine, cadmium, methyl methane sulfonate (MMS), and Spent pot liner (SPL). Millet demonstrated sensitivity and efficiency in response to these tests. In conclusion, millet proves to be an effective species for the toxicological risk assessment of environmental pollutants.

Feed intake, methane yield, and efficiency of utilization of energy and nitrogen by sheep fed tropical grasses.

Forage allowance impacts dry matter (DM) intake and the use of nutrients by ruminants. The efficient use of protein and energy from pasture is related to better livestock performance and lower environmental impacts. The aims of this study were to evaluate the effect of forage allowance levels on intake, digestibility, nitrogen (N) and energy balance, and methane (CH4) emissions by lambs fed fresh pearl millet [Pennisetum americanum (L.) Leeke]. An indoor trial was performed using lambs in a completely randomized design with four treatments [forage allowance at 1.5, 2.0, 2.5 kg DM/100 kg of live weight (LW), and ad libitum allowing 20% of refusals] and four replicates (lambs). Forage intake, digestibility, total urine and feces excretion, and CH4 emission were measured to calculate N and energy balances. An increase in forage allowance resulted in a linear increase in lamb forage intake, N retention, and metabolizable energy intake. Moreover, lamb CH4 emission (g/day) also increased with greater forage allowance, while CH4 yield decreased linearly as forage allowance increased. Our results indicate that maximizing forage intake improves N and energy use efficiency and mitigates CH4 yield and decreases CH4 conversion factor (Ym) by lambs fed pearl millet forage. Thus, management strategies that optimize intake of tropical forages by ruminants improve the use of nutrients ingested and mitigates negative impacts to the environment.

Overcoming Phosphorus Deficiency in West African Pearl Millet and Sorghum Production Systems: Promising Options for Crop Improvement.

West Africa (WA) is among the most food insecure regions. Rapid human population growth and stagnating crop yields greatly contribute to this fact. Poor soil fertility, especially low plant available phosphorus (P) is constraining food production in the region. P-fertilizer use in WA is among the lowest in the world due to inaccessibility and high prices, often unaffordable to resource-poor subsistence farmers. This article provides an overview of soil P-deficiency in WA and opportunities to overcome it by exploiting sorghum and pearl millet genetic diversity. The topic is examined from the perspectives of plant breeding, soil science, plant physiology, plant nutrition, and agronomy, thereby referring to recent results obtained in a joint interdisciplinary research project, and reported literature. Specific objectives are to summarize: (1) The global problem of P scarcity and how it will affect WA farmers; (2) Soil P dynamics in WA soils; (3) Plant responses to P deficiency; (4) Opportunities to breed for improved crop adaptation to P-limited conditions; (5) Challenges and trade-offs for improving sorghum and pearl millet adaptation to low-P conditions in WA; and (6) Systems approaches to address soil P-deficiency in WA. Sorghum and pearl millet in WA exhibit highly significant genetic variation for P-uptake efficiency, P-utilization efficiency, and grain yield under P-limited conditions indicating the possibility of breeding P-efficient varieties. Direct selection under P-limited conditions was more efficient than indirect selection under high-P conditions. Combining P-uptake and P-utilization efficiency is recommendable for WA to avoid further soil mining. Genomic regions responsible for P-uptake, P-utilization efficiency, and grain yield under low-P have been identified in WA sorghum and pearl millet, and marker-assisted selection could be possible once these genomic regions are validated. Developing P-efficient genotypes may not, however, be a sustainable solution in itself in the long-term without replenishing the P removed from the system in harvested produce. We therefore propose the use of integrated soil fertility management and systems-oriented management such as enhanced crop-tree-livestock integration in combination with P-use-efficiency-improved varieties. Recycling P from animal bones, human excreta and urine are also possible approaches toward a partially closed and efficient P cycle in WA.

Cover crops growth under water deficit / Crescimento de plantas de cobertura sob déficit hídrico

One of the challenges to be overcome in no till deploying in tropical regions is the production of straw in the offseason, a period commonly with low water availability. To help in the choice of species to be used as cover crop in dry winter regions, the aim of this work was to evaluate the effect of soil water potential on growth of black oat (Avena strigosa Sckreb), pearl millet (Pennisetum glaucum (L.) R. Brown), grain sorghum (Sorghum bicolor L. Moench.) e guinea sorghum (Sorghum bicolor subespécie bicolor raça guinea). Pearl millet is a good option to be cropped during offseason by show high yield potencial, even been more sensitive to water deficit. Grain sorghum and guinea sorghum are also good options, particularly by showed abundant root system, which possibly gives them a certain tolerance to low water availability conditions. The black oat, even with high tolerance to water stress (tolerance conferred by highest percentage of fine roots), seems to be much affected by higher temperatures, common to these regions.

Um dos desafios a serem vencidos na implantação do sistema de semeadura direta em regiões tropicais é a produção de palhada na entressafra, período comumente com baixa disponibilidade hídrica. Visando auxiliar a escolha das espécies a serem empregadas como planta de cobertura em regiões de inverno seco, objetivou-se com este trabalho avaliar o efeito de potenciais de água no solo no crescimento de aveia preta (Avena strigosa Sckreb), milheto (Pennisetum glaucum (L.) R. Brown), sorgo granífero (Sorghum bicolor L. Moench.) e sorgo-de-guiné (Sorghum bicolor subespécie bicolor raça guinea), bem como detectar possíveis estratégias destas espécies para contornarem condições de baixa disponibilidade hídrica. O milheto, mesmo sendo mais sensível ao déficit hídrico, é uma boa opção a ser cultivado na entressafra pelo seu elevado potencial produtivo. O sorgo granífero e o sorgo-de-guiné também são boas opções, em especial por apresentarem abundante sistema radicular, o que confere aos mesmos certa tolerância à condições de baixa disponibilidade hídrica. A aveia preta, mesmo apresentando aparentemente elevada tolerância à deficiência hídrica (tolerância esta conferida pela maior porcentagem de raízes finas), parece ser muito prejudicada por temperaturas mais elevadas, comuns a estas regiões.

Cover crops growth under water deficit / Crescimento de plantas de cobertura sob déficit hídrico

One of the challenges to be overcome in no till deploying in tropical regions is the production of straw in the offseason, a period commonly with low water availability. To help in the choice of species to be used as cover crop in dry winter regions, the aim of this work was to evaluate the effect of soil water potential on growth of black oat (Avena strigosa Sckreb), pearl millet (Pennisetum glaucum (L.) R. Brown), grain sorghum (Sorghum bicolor L. Moench.) e guinea sorghum (Sorghum bicolor subespécie bicolor raça guinea). Pearl millet is a good option to be cropped during offseason by show high yield potencial, even been more sensitive to water deficit. Grain sorghum and guinea sorghum are also good options, particularly by showed abundant root system, which possibly gives them a certain tolerance to low water availability conditions. The black oat, even with high tolerance to water stress (tolerance conferred by highest percentage of fine roots), seems to be much affected by higher temperatures, common to these regions.

Um dos desafios a serem vencidos na implantação do sistema de semeadura direta em regiões tropicais é a produção de palhada na entressafra, período comumente com baixa disponibilidade hídrica. Visando auxiliar a escolha das espécies a serem empregadas como planta de cobertura em regiões de inverno seco, objetivou-se com este trabalho avaliar o efeito de potenciais de água no solo no crescimento de aveia preta (Avena strigosa Sckreb), milheto (Pennisetum glaucum (L.) R. Brown), sorgo granífero (Sorghum bicolor L. Moench.) e sorgo-de-guiné (Sorghum bicolor subespécie bicolor raça guinea), bem como detectar possíveis estratégias destas espécies para contornarem condições de baixa disponibilidade hídrica. O milheto, mesmo sendo mais sensível ao déficit hídrico, é uma boa opção a ser cultivado na entressafra pelo seu elevado potencial produtivo. O sorgo granífero e o sorgo-de-guiné também são boas opções, em especial por apresentarem abundante sistema radicular, o que confere aos mesmos certa tolerância à condições de baixa disponibilidade hídrica. A aveia preta, mesmo apresentando aparentemente elevada tolerância à deficiência hídrica (tolerância esta conferida pela maior porcentagem de raízes finas), parece ser muito prejudicada por temperaturas mais elevadas, comuns a estas regiões.

Produção de forragem de gramíneas anuais semeadas no verão / Forage yield of annual grasses seeded on the summer

No Rio Grande do Sul, a base forrageira para bovinos de corte e de leite é constituída, basicamente, por pastagens de gramíneas perenes de verão, com bom valor nutritivo (VN) durante a primavera e parte do verão, quando manejadas adequadamente. Entretanto, a forragem no outono e inverno tem baixa concentração de nutrientes, o que ainda é agravado pelas geadas. Um experimento com dois anos de avaliação com parcelas divididas no delineamento em blocos casualizados, com três repetições, comparou rendimento, distribuição de forragem e valor nutritivo em três épocas de semeadura (janeiro, fevereiro e março), alocadas nas parcelas principais, e cinco genótipos (milheto comum, capim-sudão ou aveia de verão, teosinto e os híbridos de sorgo BRS 800 e AG 2501C) nas subparcelas. As duas primeiras épocas de semeadura resultaram em maior rendimento de forragem (mais de 6,0Mg ha-1 de MS) que a semeadura de março, com elevado valor nutritivo (>15% PB). Os sorgos forrageiros foram mais produtivos que capim-sudão e teosinto. Milheto, capim-sudão e teosinto têm maior afilhamento que os sorgos forrageiros híbridos. Milheto tem maior teor de PB (20%) e menor de FDA (35%) nas lâminas foliares quando comparado aos sorgos e teosinto. É possível minimizar a crise forrageira conhecida como vazio forrageiro outonal com a semeadura em múltiplas datas de forrageiras anuais de verão, até o final de fevereiro na região do Planalto Médio do RS e estender o período de pastejo em até 60 dias, em período em que as pastagens perenes de verão têm baixa oferta de forragem ou baixo valor nutritivo e as forrageiras anuais de inverno não estão estabelecidas.

In the Rio Grande do Sul (RS) state, southern Brazil, the forage foundation of beef and dairy cattle operations is pasture of warm-season grasses, with high nutritive value (NV) during spring and part of summer seasons, if managed frequently. However, during cool-season, forage NV is very low and worsen by frosts. A 2-yr split-plot experiment on randomized complete block design with three replications compared yield, yield distribution, and nutritive value in three seeding dates (January, February, and March) allocated on main plots, and five forage grasses cultivars (common pearl millet, teosinte, sudangrass, and BRS 800 and AG 2501C sorghum hybrids) on subplots. The two first seeding dates had the highest forage yield, about 6.0Mg ha-1 of DM than March seeding date of high nutritive value forage (>150g kg-1 MS). Sorghum-hybrids genotypes yield more than teosinte and sudangrass. Pearl millet, sudangrass and teosinte had more tillering. Pearl millet had high CP (200g kg-1 DM), and lower FDA (350g kg-1 DM) concentrations on leaf blades compared to sorghums and teosinte. It is possible minimize fall forage shortage seeding annual forage grasses until end of February in the Planalto region of RS state, and extend the productive period, an additional 30 to 60-d, during a time of year when warm-season perennial grasses have low forage allowance or low nutritive value, and annual winter forages are not established.

Genetic architecture of purple pigmentation and tagging of some loci to SSR markers in pearl millet, Pennisetum glaucum (L.) R. Br.

This report describes the construction of integrated genetic maps in pearl millet involving certain purple phenotype and simple sequence repeat (SSR) markers. These maps provide a direct means of implementing DNA marker-assisted selection and of facilitating "map-based cloning" for engineering novel traits. The purple pigmentation of leaf sheath, midrib and leaf margin was inherited together 'en bloc' under the control of a single dominant locus (the 'midrib complex') and was inseparably associated with the locus governing the purple coloration of the internode. The purple panicle was caused by a single dominant locus. Each of the three characters (purple lamina, purple stigma and purple seed) was governed by two complementary loci. One of the two loci governing purple seed was associated with the SSR locus Xpsmp2090 in linkage group 1, with a linkage value of 22 cM, while the other locus was associated with the SSR locus Xpsmp2270 in linkage group 6, with a linkage value of 23 cM. The locus for purple pigmentation of the midrib complex was either responsible for pigmentation of the panicle in a pleiotropic manner or was linked to it very closely and associated with the SSR locus Xpsmp2086 in linkage group 4, with a suggestive linkage value of 21 cM. A dominant allele at this locus seems to be a prerequisite for the development of purple pigmentation in the lamina, stigma and seed. These findings suggest that the locus for pigmentation of the midrib complex might regulate the basic steps in anthocyanin pigment development by acting as a structural gene while other loci regulate the formation of color in specific plant parts.

Produção de forragem de gramíneas anuais semeadas no verão

In the Rio Grande do Sul (RS) state, southern Brazil, the forage foundation of beef and dairy cattle operations is pasture of warm-season grasses, with high nutritive value (NV) during spring and part of summer seasons, if managed frequently. However, during cool-season, forage NV is very low and worsen by frosts. A 2-yr split-plot experiment on randomized complete block design with three replications compared yield, yield distribution, and nutritive value in three seeding dates (January, February, and March) allocated on main plots, and five forage grasses cultivars (common pearl millet, teosinte, sudangrass, and BRS 800 and AG 2501C sorghum hybrids) on subplots. The two first seeding dates had the highest forage yield, about 6.0Mg ha-1 of DM than March seeding date of high nutritive value forage (>150g kg-1 MS). Sorghum-hybrids genotypes yield more than teosinte and sudangrass. Pearl millet, sudangrass and teosinte had more tillering. Pearl millet had high CP (200g kg-1 DM), and lower FDA (350g kg-1 DM) concentrations on leaf blades compared to sorghums and teosinte. It is possible minimize fall forage shortage seeding annual forage grasses until end of February in the Planalto region of RS state, and extend the productive period, an additional 30 to 60-d, during a time of year when warm-season perennial grasses have low forage allowance or low nutritive value, and annual winter forages are not established.

No Rio Grande do Sul, a base forrageira para bovinos de corte e de leite é constituída, basicamente, por pastagens de gramíneas perenes de verão, com bom valor nutritivo (VN) durante a primavera e parte do verão, quando manejadas adequadamente. Entretanto, a forragem no outono e inverno tem baixa concentração de nutrientes, o que ainda é agravado pelas geadas. Um experimento com dois anos de avaliação com parcelas divididas no delineamento em blocos casualizados, com três repetições, comparou rendimento, distribuição de forragem e valor nutritivo em três épocas de semeadura (janeiro, fevereiro e março), alocadas nas parcelas principais, e cinco genótipos (milheto comum, capim-sudão ou aveia de verão, teosinto e os híbridos de sorgo BRS 800 e AG 2501C) nas subparcelas. As duas primeiras épocas de semeadura resultaram em maior rendimento de forragem (mais de 6,0Mg ha-1 de MS) que a semeadura de março, com elevado valor nutritivo (>15% PB). Os sorgos forrageiros foram mais produtivos que capim-sudão e teosinto. Milheto, capim-sudão e teosinto têm maior afilhamento que os sorgos forrageiros híbridos. Milheto tem maior teor de PB (20%) e menor de FDA (35%) nas lâminas foliares quando comparado aos sorgos e teosinto. É possível minimizar a crise forrageira conhecida como vazio forrageiro outonal com a semeadura em múltiplas datas de forrageiras anuais de verão, até o final de fevereiro na região do Planalto Médio do RS e estender o período de pastejo em até 60 dias, em período em que as pastagens perenes de verão têm baixa oferta de forragem ou baixo valor nutritivo e as forrageiras anuais de inverno não estão estabelecidas.

Produção de forragem de gramíneas anuais semeadas no verão

In the Rio Grande do Sul (RS) state, southern Brazil, the forage foundation of beef and dairy cattle operations is pasture of warm-season grasses, with high nutritive value (NV) during spring and part of summer seasons, if managed frequently. However, during cool-season, forage NV is very low and worsen by frosts. A 2-yr split-plot experiment on randomized complete block design with three replications compared yield, yield distribution, and nutritive value in three seeding dates (January, February, and March) allocated on main plots, and five forage grasses cultivars (common pearl millet, teosinte, sudangrass, and BRS 800 and AG 2501C sorghum hybrids) on subplots. The two first seeding dates had the highest forage yield, about 6.0Mg ha-1 of DM than March seeding date of high nutritive value forage (>150g kg-1 MS). Sorghum-hybrids genotypes yield more than teosinte and sudangrass. Pearl millet, sudangrass and teosinte had more tillering. Pearl millet had high CP (200g kg-1 DM), and lower FDA (350g kg-1 DM) concentrations on leaf blades compared to sorghums and teosinte. It is possible minimize fall forage shortage seeding annual forage grasses until end of February in the Planalto region of RS state, and extend the productive period, an additional 30 to 60-d, during a time of year when warm-season perennial grasses have low forage allowance or low nutritive value, and annual winter forages are not established.

No Rio Grande do Sul, a base forrageira para bovinos de corte e de leite é constituída, basicamente, por pastagens de gramíneas perenes de verão, com bom valor nutritivo (VN) durante a primavera e parte do verão, quando manejadas adequadamente. Entretanto, a forragem no outono e inverno tem baixa concentração de nutrientes, o que ainda é agravado pelas geadas. Um experimento com dois anos de avaliação com parcelas divididas no delineamento em blocos casualizados, com três repetições, comparou rendimento, distribuição de forragem e valor nutritivo em três épocas de semeadura (janeiro, fevereiro e março), alocadas nas parcelas principais, e cinco genótipos (milheto comum, capim-sudão ou aveia de verão, teosinto e os híbridos de sorgo BRS 800 e AG 2501C) nas subparcelas. As duas primeiras épocas de semeadura resultaram em maior rendimento de forragem (mais de 6,0Mg ha-1 de MS) que a semeadura de março, com elevado valor nutritivo (>15% PB). Os sorgos forrageiros foram mais produtivos que capim-sudão e teosinto. Milheto, capim-sudão e teosinto têm maior afilhamento que os sorgos forrageiros híbridos. Milheto tem maior teor de PB (20%) e menor de FDA (35%) nas lâminas foliares quando comparado aos sorgos e teosinto. É possível minimizar a crise forrageira conhecida como vazio forrageiro outonal com a semeadura em múltiplas datas de forrageiras anuais de verão, até o final de fevereiro na região do Planalto Médio do RS e estender o período de pastejo em até 60 dias, em período em que as pastagens perenes de verão têm baixa oferta de forragem ou baixo valor nutritivo e as forrageiras anuais de inverno não estão estabelecidas.

Produção de forragem de gramíneas anuais semeadas no verão

In the Rio Grande do Sul (RS) state, southern Brazil, the forage foundation of beef and dairy cattle operations is pasture of warm-season grasses, with high nutritive value (NV) during spring and part of summer seasons, if managed frequently. However, during cool-season, forage NV is very low and worsen by frosts. A 2-yr split-plot experiment on randomized complete block design with three replications compared yield, yield distribution, and nutritive value in three seeding dates (January, February, and March) allocated on main plots, and five forage grasses cultivars (common pearl millet, teosinte, sudangrass, and BRS 800 and AG 2501C sorghum hybrids) on subplots. The two first seeding dates had the highest forage yield, about 6.0Mg ha-1 of DM than March seeding date of high nutritive value forage (>150g kg-1 MS). Sorghum-hybrids genotypes yield more than teosinte and sudangrass. Pearl millet, sudangrass and teosinte had more tillering. Pearl millet had high CP (200g kg-1 DM), and lower FDA (350g kg-1 DM) concentrations on leaf blades compared to sorghums and teosinte. It is possible minimize fall forage shortage seeding annual forage grasses until end of February in the Planalto region of RS state, and extend the productive period, an additional 30 to 60-d, during a time of year when warm-season perennial grasses have low forage allowance or low nutritive value, and annual winter forages are not established.

No Rio Grande do Sul, a base forrageira para bovinos de corte e de leite é constituída, basicamente, por pastagens de gramíneas perenes de verão, com bom valor nutritivo (VN) durante a primavera e parte do verão, quando manejadas adequadamente. Entretanto, a forragem no outono e inverno tem baixa concentração de nutrientes, o que ainda é agravado pelas geadas. Um experimento com dois anos de avaliação com parcelas divididas no delineamento em blocos casualizados, com três repetições, comparou rendimento, distribuição de forragem e valor nutritivo em três épocas de semeadura (janeiro, fevereiro e março), alocadas nas parcelas principais, e cinco genótipos (milheto comum, capim-sudão ou aveia de verão, teosinto e os híbridos de sorgo BRS 800 e AG 2501C) nas subparcelas. As duas primeiras épocas de semeadura resultaram em maior rendimento de forragem (mais de 6,0Mg ha-1 de MS) que a semeadura de março, com elevado valor nutritivo (>15% PB). Os sorgos forrageiros foram mais produtivos que capim-sudão e teosinto. Milheto, capim-sudão e teosinto têm maior afilhamento que os sorgos forrageiros híbridos. Milheto tem maior teor de PB (20%) e menor de FDA (35%) nas lâminas foliares quando comparado aos sorgos e teosinto. É possível minimizar a crise forrageira conhecida como vazio forrageiro outonal com a semeadura em múltiplas datas de forrageiras anuais de verão, até o final de fevereiro na região do Planalto Médio do RS e estender o período de pastejo em até 60 dias, em período em que as pastagens perenes de verão têm baixa oferta de forragem ou baixo valor nutritivo e as forrageiras anuais de inverno não estão estabelecidas.

Genetic architecture of purple pigmentation and tagging of some loci to SSR markers in pearl millet, Pennisetum glaucum (L.) R. Br.

This report describes the construction of integrated genetic maps in pearl millet involving certain purple phenotype and simple sequence repeat (SSR) markers. These maps provide a direct means of implementing DNA marker-assisted selection and of facilitating "map-based cloning" for engineering novel traits. The purple pigmentation of leaf sheath, midrib and leaf margin was inherited together 'en bloc' under the control of a single dominant locus (the 'midrib complex') and was inseparably associated with the locus governing the purple coloration of the internode. The purple panicle was caused by a single dominant locus. Each of the three characters (purple lamina, purple stigma and purple seed) was governed by two complementary loci. One of the two loci governing purple seed was associated with the SSR locus Xpsmp2090 in linkage group 1, with a linkage value of 22 cM, while the other locus was associated with the SSR locus Xpsmp2270 in linkage group 6, with a linkage value of 23 cM. The locus for purple pigmentation of the midrib complex was either responsible for pigmentation of the panicle in a pleiotropic manner or was linked to it very closely and associated with the SSR locus Xpsmp2086 in linkage group 4, with a suggestive linkage value of 21 cM. A dominant allele at this locus seems to be a prerequisite for the development of purple pigmentation in the lamina, stigma and seed. These findings suggest that the locus for pigmentation of the midrib complex might regulate the basic steps in anthocyanin pigment development by acting as a structural gene while other loci regulate the formation of color in specific plant parts.

Characterization of interphase nuclei from triploid hybrids between Pennisetum purpureum Schumach and Pennisetum glaucum (L.) R. Br. / Caracterização de núcleos interfásicos de híbridos tríploides entre Pennisetum purpureum Schumach e Pennisetum glaucum (L.) R. Br.

This study evaluated the structure and the volume of interphase nuclei from root meristems of the genotypes of napier grass (Pennisetum purpureum), pearl millet (Pennisetum glaucum) and hybrids resultant of such breeding. In napier grass, nuclei were areticulate. Both pearl millet and the triploid hybrid had semi-reticulate nuclei; also, the hybrid presented a small proportion (6 percent) of areticulate nuclei. Pearl millet had the highest averages of nuclear dimensions, such as volume, diameter and radius, followed by the interspecific hybrid and napier grass. There was no intraspecific variation for the type of nuclear structure, which indicates this feature is important for cytotaxonomic studies involving the genus Pennisetum. Results demonstrated that chromatin organization in these nuclei was influenced by the number and size of chromosomes, affecting the nucleus volume in the analyzed taxa.

Avaliou-se, neste estudo, a estrutura e o volume de núcleos interfásicos de meristemas radiculares de genótipos do capim-elefante (Pennisetum purpureum), milheto (Pennisetum glaucum) e do híbrido resultante deste cruzamento. O capim-elefante apresentou núcleos do tipo arreticulado. Tanto no milheto como no híbrido triplóide os núcleos apresentaram-se semi-reticulados, sendo que, no híbrido, foi observada uma pequena proporção (6 por cento) de núcleos arreticulados. As maiores médias para as dimensões nucleares como volume nuclear, diâmetro e raio foram obtidas para o milheto, seguidas do híbrido interespecífico e capim-elefante. Não houve variação intraespecífica para tipo de estrutura nuclear indicando que essa característica tem relevância para estudos citotaxonômicos no gênero Pennisetum. Os resultados demonstraram que a organização da cromatina nesses núcleos foi influenciada pelo número e tamanho dos cromossomos e essa afetou o volume nuclear dos táxons analisados.

Cinética de fermentação ruminal de silagens de milheto / Ruminal fermentation kinetics of pearl millet silages

Avaliaram-se a degradabilidade da matéria seca e a cinética de fermentação ruminal das silagens de três genótipos de milheto (BRS-1501, NPM-1 e CMS-3), por meio da técnica in vitro semi-automática de produção de gases. O delineamento experimental utilizado foi o de blocos ao acaso em arranjo de parcelas subdivididas. Para a comparação dos genótipos, foi utilizado o teste SNK (P<0,05), e os parâmetros da cinética de fermentação foram ajustados ao modelo unicompartimental de France. A partir de 24 horas, a silagem do genótipo BRS-1501 foi a que apresentou maior produção acumulativa de gases. Os valores de degradabilidade da matéria seca apresentados às 96 horas de fermentação foram de 53,9 por cento para a silagem do BRS-1501, 51,7 por cento para a silagem do CMS-3 e 49,1 por cento para a silagem do NPM-1, sendo este o material com menor degradabilidade (P<0,05). Os potenciais máximos de produção de gases variaram de 145,18 para o genótipo CMS-3 a 155,02ml/g de MS para o BRS-1501. A silagem do genótipo BRS-1501 destacou-se entre as demais, em função do seu maior potencial de produção de gases e degradabilidades efetivas.(AU)

The dry matter digestibility and the fermentation kinetics of three pearl millet genotypes silages (BRS-1501, NPM-1, and CMS-3) were estimated by the semi-automated in vitro gas production technique, using a completely randomized blocks design in a split plot arrangement. The genotypes means were compared by SNK test (P<0.05) and fermentation kinetics parameters estimated by regression of gas production on time using the France model. The greater cumulative gas production from 24 hours on was obtained by BRS-1501 genotype silage. The dry matter degradability (DMD) values at 96 hours of incubation were 53.9 percent for BRS-1501, 51.7 percent for NPM-1, and 49.1 percent for CMS-3 silage. The lowest DMD (P<0.05) among the studied genotypes silages was observed for CMS-3 silage. The maximum potential of gas production ranged from 145.18ml/g of dry matter for CMS-3 genotype silage to 155.02ml/g of dry matter for BRS-1501 silage. The BRS-1501 genotype silage showed the highest potential of gas production and effective degradabilities.(AU)

Cinética de fermentação ruminal de silagens de milheto / Ruminal fermentation kinetics of pearl millet silages

Avaliaram-se a degradabilidade da matéria seca e a cinética de fermentação ruminal das silagens de três genótipos de milheto (BRS-1501, NPM-1 e CMS-3), por meio da técnica in vitro semi-automática de produção de gases. O delineamento experimental utilizado foi o de blocos ao acaso em arranjo de parcelas subdivididas. Para a comparação dos genótipos, foi utilizado o teste SNK (P<0,05), e os parâmetros da cinética de fermentação foram ajustados ao modelo unicompartimental de France. A partir de 24 horas, a silagem do genótipo BRS-1501 foi a que apresentou maior produção acumulativa de gases. Os valores de degradabilidade da matéria seca apresentados às 96 horas de fermentação foram de 53,9 por cento para a silagem do BRS-1501, 51,7 por cento para a silagem do CMS-3 e 49,1 por cento para a silagem do NPM-1, sendo este o material com menor degradabilidade (P<0,05). Os potenciais máximos de produção de gases variaram de 145,18 para o genótipo CMS-3 a 155,02ml/g de MS para o BRS-1501. A silagem do genótipo BRS-1501 destacou-se entre as demais, em função do seu maior potencial de produção de gases e degradabilidades efetivas.

The dry matter digestibility and the fermentation kinetics of three pearl millet genotypes silages (BRS-1501, NPM-1, and CMS-3) were estimated by the semi-automated in vitro gas production technique, using a completely randomized blocks design in a split plot arrangement. The genotypes means were compared by SNK test (P<0.05) and fermentation kinetics parameters estimated by regression of gas production on time using the France model. The greater cumulative gas production from 24 hours on was obtained by BRS-1501 genotype silage. The dry matter degradability (DMD) values at 96 hours of incubation were 53.9 percent for BRS-1501, 51.7 percent for NPM-1, and 49.1 percent for CMS-3 silage. The lowest DMD (P<0.05) among the studied genotypes silages was observed for CMS-3 silage. The maximum potential of gas production ranged from 145.18ml/g of dry matter for CMS-3 genotype silage to 155.02ml/g of dry matter for BRS-1501 silage. The BRS-1501 genotype silage showed the highest potential of gas production and effective degradabilities.

Pearl millet utilization in ccommercial laying hen diets formulated on a total or digestible amino acid basis

An experiment was carried out to evaluate the effect of replacing corn with pearl millet in commercial layer diets, formulated according to the minimal requirements for total and digestible amino acids. Two hundred and forty Lohmann LSL laying hens with 25 weeks of age were distributed in a completely randomized experimental design according to a 2 x 5 factorial arrangement with 3 replicates of 8 birds. Feed was formulated on two amino acid basis (total or digestible) according to Rostagno et al. (2000) and there were five pearl millet inclusion levels (0%, 25%, 50%, 75%, and 100%). Performance and egg quality were evaluated during five periods of 21 days.At the end of each period, feed intake, egg production, egg weight and feed conversion were evaluated. In the last three days of each period, the following egg quality parameters were evaluated: Haugh Unit, yolk pigmentation index, egg specific weight, shell percentage and shell thickness. Digestible amino acid requirements resulted in decreased feed intake (p 0.01) and increased production costs per mass of eggs (kg) or per dozen eggs (p 0.01) compared to total amino acid requirements. There was a linear reduction in feed intake, egg production, egg weight and yolk pigmentation index with increasing inclusion levels of pearl millet. Therefore, increasing levels of replacement of corn by pearl millet affected bird performance negatively. Besides, production costs were higher with increasing pearl millet levels.

Pearl millet utilization in ccommercial laying hen diets formulated on a total or digestible amino acid basis

An experiment was carried out to evaluate the effect of replacing corn with pearl millet in commercial layer diets, formulated according to the minimal requirements for total and digestible amino acids. Two hundred and forty Lohmann LSL laying hens with 25 weeks of age were distributed in a completely randomized experimental design according to a 2 x 5 factorial arrangement with 3 replicates of 8 birds. Feed was formulated on two amino acid basis (total or digestible) according to Rostagno et al. (2000) and there were five pearl millet inclusion levels (0%, 25%, 50%, 75%, and 100%). Performance and egg quality were evaluated during five periods of 21 days.At the end of each period, feed intake, egg production, egg weight and feed conversion were evaluated. In the last three days of each period, the following egg quality parameters were evaluated: Haugh Unit, yolk pigmentation index, egg specific weight, shell percentage and shell thickness. Digestible amino acid requirements resulted in decreased feed intake (p 0.01) and increased production costs per mass of eggs (kg) or per dozen eggs (p 0.01) compared to total amino acid requirements. There was a linear reduction in feed intake, egg production, egg weight and yolk pigmentation index with increasing inclusion levels of pearl millet. Therefore, increasing levels of replacement of corn by pearl millet affected bird performance negatively. Besides, production costs were higher with increasing pearl millet levels.

Milheto-grão (Pennisetum glaucum (L.) R.Br.) como substituto do milho em rações para poedeiras comerciais

The production performance and egg quality of laying hens receiving increasing levels of pearl millet grain in rations were studied. Two hundred 30-week-old LSL Lohmann white hens, allotted in a completely randomized design with five treatments, five replicates with 10 hens, were fed with 0, 25, 50, 75 and 100% of pearl millet grain in rations as corn substitute. The performance was evaluated by egg production, feed intake, feed conversion in terms of dozen eggs and egg mass, and egg quality measured as egg weight, shell, albumen and yolk porcentages. No differences among treatments were observed for all characteristics, but the yolk color index measured by the Roche fan indicated decreasing in yolk colour as the level of pearl millet increased. Pearl millet did not affect the production performance, and a synthetic or natural pigment should be included in rations.

Avaliaram-se o desempenho produtivo e a qualidade dos ovos de poedeiras comerciais alimentadas com milheto como substituto do milho em rações de postura. Duzentas poedeiras comerciais, da linhagem LSL Lohmann branca, com 30 semanas de idade, receberam rações com níveis crescentes de substituição da energia metabolizável fornecida pelo milho por milheto. As aves foram distribuídas em um delineamento inteiramente ao acaso com cinco tratamentos, cinco repetições e 10 aves por repetição. Foram avaliados a porcentagem de postura, o consumo de ração, a conversão alimentar em termos de dúzias e de massa de ovos, o peso médio de ovos, a porcentagem de casca e de gema, e a porcentagem de clara. Não se observou diferença estatística significativa em todas as características. O índice de coloração de gema, pelo leque colorimétrico Roche, apresentou pior coloração à medida que os níveis de milheto foram crescentes nas rações. Os resultados indicam que o milheto não afetou o desempenho produtivo das aves, devendo-se recomendar o seu uso com pigmentantes sintéticos ou naturais.

Milheto (Pennisetum glaucum (L.) R.Br.) como substituto do milho em rações para codornas-japonesas em postura (Coturnix coturnix japonica)

The production performance of Japanese quails receiving increasing levels of pearl millet grain in rations was studied. Two hundred, 70-day-old quails submitted to a completely randomized design with five treatments and four replicates with ten birds each, were fed with 0, 25, 50, 75 and 100% of pearl millet grain in laying rations. The production and weight of eggs and the feed intake, the mortality rate and yolk color by the use of colorimetric fan were measured, and the feed conversion per dozen eggs and in relation to egg mass were calculated. The results indicated that the production performance of laying quails was not affected by the use of increasing levels of pearl millet grain and that pearl millet is a good corn substitute for Japanese quail, but a yolk pigment should be used in the ration.

Avaliou-se o desempenho de codornas-japonesas em postura alimentadas com níveis crescentes de milheto-grão na ração. Duzentas codornas com 70 dias de idade, submetidas a um delineamento inteiramente ao acaso, com cinco tratamentos e quatro repetições de 10 aves, receberam níveis de milheto de 0, 25, 50, 75 e 100% em substituição ao milho em rações na fase de postura. Foram medidos a produção e o peso dos ovos, o consumo de ração, a mortalidade, a coloração da gema pelo leque colorimétrico, e calculada a conversão alimentar em termos de massa e de dúzia de ovos. Os índices de produtividade das codornas não foram afetados pelos diferentes níveis de substituição estudados e o milheto-grão se constituiu em uma fonte de substituição do milho para codornas-japonesas, devendo-se, porém, incluir na ração uma fonte de pigmentação para a gema dos ovos.

Milheto-grão (Pennisetum glaucum (L.) R.Br.) como substituto do milho em rações para poedeiras comerciais

The production performance and egg quality of laying hens receiving increasing levels of pearl millet grain in rations were studied. Two hundred 30-week-old LSL Lohmann white hens, allotted in a completely randomized design with five treatments, five replicates with 10 hens, were fed with 0, 25, 50, 75 and 100% of pearl millet grain in rations as corn substitute. The performance was evaluated by egg production, feed intake, feed conversion in terms of dozen eggs and egg mass, and egg quality measured as egg weight, shell, albumen and yolk porcentages. No differences among treatments were observed for all characteristics, but the yolk color index measured by the Roche fan indicated decreasing in yolk colour as the level of pearl millet increased. Pearl millet did not affect the production performance, and a synthetic or natural pigment should be included in rations.

Avaliaram-se o desempenho produtivo e a qualidade dos ovos de poedeiras comerciais alimentadas com milheto como substituto do milho em rações de postura. Duzentas poedeiras comerciais, da linhagem LSL Lohmann branca, com 30 semanas de idade, receberam rações com níveis crescentes de substituição da energia metabolizável fornecida pelo milho por milheto. As aves foram distribuídas em um delineamento inteiramente ao acaso com cinco tratamentos, cinco repetições e 10 aves por repetição. Foram avaliados a porcentagem de postura, o consumo de ração, a conversão alimentar em termos de dúzias e de massa de ovos, o peso médio de ovos, a porcentagem de casca e de gema, e a porcentagem de clara. Não se observou diferença estatística significativa em todas as características. O índice de coloração de gema, pelo leque colorimétrico Roche, apresentou pior coloração à medida que os níveis de milheto foram crescentes nas rações. Os resultados indicam que o milheto não afetou o desempenho produtivo das aves, devendo-se recomendar o seu uso com pigmentantes sintéticos ou naturais.