RESUMO
OBJECTIVE: To determine and optimize the sensitivity of the CellaVision DM96 automated image-analysis system in detecting platelet (PLT) clumps on blood smears and to assess the reliability of the traditional laboratory practice of examining only the feather edge of the smear for PLT clumps. METHODS: We processed 102 blood smears that revealed PLT clumps on microscopic review, using the CellaVision DM96, and reviewed the results for the ability of the analyzer to detect these clumps. We obtained the data regarding relative distribution of PLT clumps on different parts of the blood smear (feather edge, lateral edges, and readable area) from our microscopic-review observations. RESULTS: The sensitivity of the Cellavision DM96 in detecting PLT clumps was between 40.4% and 82.8%, depending on the number of screens reviewed for this variable. Via microscopic review of the smears, the PLT-clump detection rate increased from 85.3%, obtained by examining only the feather edge, to 99.0%, obtained by examining the feather edge plus the readable area. CONCLUSION: The sensitivity of the DM96 for detecting PLT clumps can be maximized to 82.8% by reviewing the entire white blood cell screen and the entire PLT screen. Microscopic review of the blood smears yielded a PLT-clump detection rate of 99.0% when we examined the feather edge and the readable area of the smear.