Existence of rare actinobacterial forms in the Indian sector of Southern Ocean: 16 S rRNA based metabarcoding study.

The significance of the Southern Ocean (SO) as a sink of atmospheric CO2 and other greenhouse gases is well established. Earlier studies have highlighted the role of microbes in various SO ecosystem processes. However, the diversity and role of actinobacteria in the Indian sector of SO (ISO) water and sediments are unknown. This study aimed to analyze the diversity of actinobacteria in water and sediment samples of SO based on amplicon microbiome analyses. The taxonomic analysis identified a total number of 27 phyla of which Proteobacteria (40.2%), Actinobacteria (13.6%), and Firmicutes (8.7%) were found to be dominant. The comparative study of water and sediment samples revealed the dominance of different actinobacteria in water and sediments. While the order Streptomycetales was dominant in the water samples, Micrococcales was found to be dominant in the sediment samples. The genus level analysis found the presence of eight and seventeen genera in the sediment and water samples, respectively. The genus Streptomyces, Saccharopolyspora, Nocardioides, Sva0996 marine group, and Mycobacterium were seen both in sediment and water samples. Marmoricola, Ilumatobacter, and Glaciihabitans were observed only in sediment samples whereas Rhodococcus, Corynebacterium, Micrococcus, Turicella, Pseudonocardia, Bifidobacterium, Nesterenkonia, Collinsella, Knoellia, Cadidatus, Actinomarina, Libanicoccus and Cutibacterium were noticed exclusively in water samples. Our study also emphasizes the need for further detailed study to understand the links between actinobacterial diversity and their ecological functions in the ISO. The available metabarcoding data paves the way for future research in cultivable forms of novel and rare Actinobacteria for their bioprospecting applications.

Atención primaria de salud en adultos mayores del Centro de Salud Belén, Loja (Ecuador) / Primary health care in older adults of the Belén Health Center, Loja (Ecuador)

La calidad de atención primaria de salud parte desde la perspectiva individual del servicio recibido en cualquiera de los niveles de atención de salud. La presente investigación tuvo como finalidad identificar el tipo de experiencia de los adultos mayores que acuden al primer nivel de atención primaria del Centro de Salud Belén en el periodo junio - diciembre 2022. Con un enfoque cuantitativo en una población de 91 adultos mayores, a quienes se aplicó el Cuestionario Herramientas de Evaluación de Atención Primaria (PCAT ­ usuarios adultos) donde la experiencia ata en la calidad de atención dentro de la calificación general (≥ 6,6; < 6,6), obtuvo predominio en el sexo femenino 52,75% (n=48) con respecto al 47,25% (n=43) del sexo masculino. La confiabilidad del cuestionario empleado se midió utilizando la prueba estadística de alfa de Cronbach con un valor de 0.79, considerado aceptable. Con los resultados obtenidos se evidencian que la calificación global de la calidad de atención fue alta sin embargo, existen deficiencias en los atributos de la atención primaria dentro del centro, además de un predominio en la consulta de la población femenina con todos los grupos etarios acude con mayor frecuencia con respecto a la masculina (AU)

The quality of primary health care starts from the individual perspective of the service received at any of the levels of health care. The purpose of this research was to identify the type of experience of older adults who attend the first level of primary care at the Belén Health Center in the period June - December 2022. With a quantitative approach in a population of 91 older adults, whom The Primary Care Assessment Tools Questionnaire (PCAT ­ adult users) was applied where experience ties in the quality of care within the general rating (≥ 6.6; < 6.6), obtaining a predominance in the female sex 52,75% (n=48) compared to 47.25% (n=43) of the male sex . The reliability of the questionnaire used was measured using the Cronbach's alpha statistical test with a value of 0.79, considered acceptable. The results obtained showed that the overall rating of the quality of care was high, however, there are deficiencies in the attributes of primary care within the Belén Health Center, in addition to a predominance in the consultation of the female population with all the age groups attend more frequently compared to men (AU)

Limited potexvirus diversity in eastern Gulf of Mexico seagrass meadows.

Turtlegrass virus X, which infects the seagrass Thalassia testudinum, is the only potexvirus known to infect marine flowering plants. We investigated potexvirus distribution in seagrasses using a degenerate reverse transcription polymerase chain reaction (RT-PCR) assay originally designed to capture potexvirus diversity in terrestrial plants. The assay, which implements Potex-5 and Potex-2RC primers, successfully amplified a 584 nt RNA-dependent RNA polymerase (RdRp) fragment from TVX-infected seagrasses. Following validation, we screened 74 opportunistically collected, apparently healthy seagrass samples for potexviruses using this RT-PCR assay. The survey examined the host species T. testudinum, Halodule wrightii, Halophila stipulacea, Syringodium filiforme, Ruppia maritima, and Zostera marina. Potexvirus PCR products were successfully generated only from T. testudinum samples and phylogenetic analysis of sequenced PCR products revealed five distinct TVX sequence variants. Although the RT-PCR assay revealed limited potexvirus diversity in seagrasses, the expanded geographic distribution of TVX shown here emphasizes the importance of future studies to investigate T. testudinum populations across its native range and understand how the observed fine-scale genetic diversity affects host-virus interactions.

Root Canal Treatment of Mandibular First Premolar with 3 Canals: A Case Report / Tratamiento Endodóntico de Primer Premolar Mandibular con 3 Canales: Reporte de un Caso

SUMMARY: The mandibular first premolar is commonly a single-rooted tooth with occasional presentation of radicular variations. This tooth usually has one root with only one canal (97.9 %). Presence of three canals is very rare. Anomalies may appear during odontogenesis which can lead to anatomical variations in teeth. Similarly, these variations may be associated with characteristics that can be attributed to specific population groups. Due to their low frequency, these additional canals can easily be missed. For this reason, a meticulous knowledge of tooth morphology and their possible anatomical variations are necessary, and the presence of extra roots and canals should be always considered before initiation of root canal treatment. Additionally, different radiographs and appropriated access cavity preparation is needed to ensure a success endodontic treatment. This article reports a diagnosis and endodontic treatment of a mandibular first premolar with three canals.

El primer premolar mandibular suele ser un diente unirradicular con presentación ocasional de variaciones radiculares. Este diente suele tener una raíz con un solo conducto (97,9 %). La presencia de tres canales es muy rara. Durante la odontogénesis pueden aparecer anomalías que pueden provocar variaciones anatómicas en los dientes. De manera similar, estas variaciones pueden estar asociadas con características que pueden atribuirse a grupos de población específicos. Debido a su baja frecuencia, estos canales adicionales pueden pasar desapercibidos fácilmente. Por esta razón, es necesario un conocimiento meticuloso de la morfología dental y sus posibles variaciones anatómicas, y siempre se debe considerar la presencia de raíces y conductos adicionales antes de iniciar el tratamiento de conducto. Además, se necesitan diferentes radiografías y una preparación adecuada de la cavidad de acceso para garantizar un tratamiento de endodoncia exitoso. Este artículo reporta un diagnóstico y tratamiento endodóntico de un primer premolar mandibular con tres canales.

Design and Validation of Primer Sets for the Detection and Quantification of Antibiotic Resistance Genes in Environmental Samples by Quantitative PCR.

The high prevalence of antibiotic resistant bacteria (ARB) in several environments is a great concern threatening human health. Particularly, wastewater treatment plants (WWTP) become important contributors to the dissemination of ARB to receiving water bodies, due to the inefficient management or treatment of highly antibiotic-concentrated wastewaters. Hence, it is vital to develop molecular tools that allow proper monitoring of the genes encoding resistances to these important therapeutic compounds (antibiotic resistant genes, ARGs). For an accurate quantification of ARGs, there is a need for sensitive and robust qPCR assays supported by a good design of primers and validated protocols. In this study, eleven relevant ARGs were selected as targets, including aadA and aadB (conferring resistance to aminoglycosides); ampC, blaTEM, blaSHV, and mecA (resistance to beta-lactams); dfrA1 (resistance to trimethoprim); ermB (resistance to macrolides); fosA (resistance to fosfomycin); qnrS (resistance to quinolones); and tetA(A) (resistance to tetracyclines). The in silico design of the new primer sets was performed based on the alignment of all the sequences of the target ARGs (orthology grade > 70%) deposited in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, allowing higher coverages of the ARGs' biodiversity than those of several primers described to date. The adequate design and performance of the new molecular tools were validated in six samples, retrieved from both natural and engineered environments related to wastewater treatment. The hallmarks of the optimized qPCR assays were high amplification efficiency (> 90%), good linearity of the standard curve (R2 > 0.980), repeatability and reproducibility across experiments, and a wide linear dynamic range. The new primer sets and methodology described here are valuable tools to upgrade the monitorization of the abundance and emergence of the targeted ARGs by qPCR in WWTPs and related environments.

Evaluation of the root morphology of mandibular first premolars using cone-beam computed tomography in a peruvian population / Evaluación de la morfología radicular de primeros premolares mandibulares mediante tomografía computarizada de haz cónico en una población peruana

Introduction: The morphology of the root canal of the first premolars is not always the same and therefore a good knowledge of its dental anatomy is essential. Aim: To assess the morphology of roots and root canals of mandibular first premolars in a Peruvian population using cone-beam computed tomography (CBCT). Materials and Methods: This was a descriptive cross-sec-tional study. A total of 370 mandibular first premolars fulfilling the inclusion criteria were evaluated using CBCT, and the number of roots and root canals, the Vertucci's classification of root canal configuration, age, sex and side of the tooth were registered. Results: One and two roots were presented in 96.2% (n=356) and 3.8% (n=14), respectively, of the mandibular first premolars analyzed, and one canal was present in 67.6% (n=250) and two canals in 32.2% (n=119). A type I root canal configuration was found in 67.6% (n=250) of the cases followed by type V with 26.2% (n=97). A statistically significant association was found between the number of roots and canals (p<0.001) and age also had a significant influence on this variable (p=0.0043). Conclusions: The presence of one canal in mandibular first premolars is the most frequent, although there is a considerable prevalence of two in the population studied. The number of roots is associated with the number of canals, with age having a significant influence on these variables.

Introducción: La morfología del canal radicular de los primeros premolares no siempre es la misma y por ello es fundamental un buen conocimiento de su anatomía dental. Objetivo: Evaluar la morfología de las raíces y conductos radiculares de primeros premolares mandibulares en una población peruana mediante tomografía computarizada de haz cónico. Materiales y Métodos: Este fue un estudio transversal descriptivo. Se evaluaron mediante tomografías un total de 370 primeros premolares mandibulares que cumplían con los criterios de inclusión, y se registró el número de raíces y conductos radiculares, la clasificación de Vertucci de la configuración radicular, la edad, el sexo y el lado del diente. Se realizaron las pruebas de chi-cuadrado y una regresión logística binaria (p<0,05). Resultado: Se presentó una y dos raíces en el 96,2% (n=356) y 3,8% (n=14), respectivamente, de los primeros premolares mandibulares analizados, y un canal estuvo presente en el 67,6% (n=250) y dos canales en el 32,2% (n=119). Se encontró una configuración del conducto radicular tipo I en el 67,6% (n=250) de los casos seguido del tipo V con un 26,2% (n=97). Se encontró una asociación estadísticamente significativa entre el número de raíces y conductos (p<0.001) y la edad también influyó significativamente en esta variable (p=0.0043). Conclusión: La presencia de 1 canal en primeros premolares mandibulares es la más frecuente, aunque existe una prevalencia considerable de 2 en la población estudiada. El número de raíces está asociado al número de canales, teniendo la edad una influencia significativa en estas variables.

Costo y porcentaje de gasto en salud por COVID-19 en primer nivel de atención / Cost and percentage of health spending by COVID-19 at the first level of care

Antecedentes. Ante la pandemia de COVID-19 el sistema de salud reasignó recursos económicos para la atención. Objetivo. Determinar el costo de la atención y el porcentaje del gasto en salud por COVID-19 en una unidad de medicina familiar de primer nivel de atención. Metodología. Estudio de costo y porcentaje de gasto en COVID-19 en una unidad de primer nivel de atención. Se identificaron los servicios generales y finales, para construir el costo fijo se utilizó la técnica de tiempos y movimientos, se identificaron el total de partidas presupuestales ejercidas en la unidad médica para cada uno de los servicios, para desagregar el gasto de los servicios generales a los finales se construyeron ponderadores. El costo variable se realizó con la técnica consenso de expertos y microcosteo. El costo promedio se relacionó con la productividad por servicio y con el total de pacientes atendidos por COVID-19, el resultado se relacionó con el presupuesto ejercido de la unidad. Resultados. El costo anual de la atención de COVID-19 en módulo respiratorio fue 158.597,25 dólares americanos, en medicina familiar fue 192.549,36 dólares americanos, el costo total ejercido en el año 2021 para atención de SARS COV 2 en una unidad de primera atención fue 351.146,61 dólares americanos. Esta cantidad representa el 9,6 % del gasto en salud. Conclusión. El costo en atención de COVID-19 y el porcentaje del gasto en salud en primer nivel de atención es elevado (AU)

Background. In the COVID-19 pandemic, the health system reallocated financial resources for care. Objetive. To determine the cost of care and the percentage of health spending due to COVID-19 in a first level care family medicine unit. Metodology. Study of the cost and percentage of spending on COVID-19 in a first-level care unit. The general and final services were identified, to construct the fixed cost, the technique of times and movements was used, the total budget items exercised in the medical unit for each of the services were identified, to disaggregate the expense of general services to the endings were constructed weights. Variable costing was performed using the expert consensus technique and microcosting. The average cost was related to productivity per service and to the total number of patients treated for COVID-19, the result was related to the budget used by the unit. Results. The annual cost of COVID-19 care in the respiratory module was 158.597,25 US dollars, in family medicine it was 192.549,36 US dollars, the total cost incurred in 2021 for SARS COV 2 care in a unit of first attention was 351.146,61 US dollars. This amount represents 9,6% of health spending. Conclusion. The cost of COVID-19 care and the percentage of health spending at the first level of care is high (AU)

The Archaea domain: Exploring historical and contemporary perspectives with in silico primer coverage analysis for future research in Dentistry.

OBJECTIVE: The complete picture of how the human microbiome interacts with its host is still largely unknown, particularly concerning microorganisms beyond bacteria. Although existing in very low abundance and not directly linked to causing diseases, archaea have been detected in various sites of the human body, including the gastrointestinal tract, oral cavity, skin, eyes, respiratory and urinary systems. But what exactly are these microorganisms? In the early 1990 s, archaea were classified as a distinct domain of life, sharing a more recent common ancestor with eukaryotes than with bacteria. While archaea's presence and potential significance in Dentistry remain under-recognized, there are concerns that they may contribute to oral dysbiosis. However, detecting archaea in oral samples presents challenges, including difficulties in culturing, the selection of DNA extraction methods, primer design, bioinformatic analysis, and databases. DESIGN: This is a comprehensive review on the oral archaeome, presenting an in-depth in silico analysis of various primers commonly used for detecting archaea in human body sites. RESULTS: Among several primer pairs used for detecting archaea in human samples across the literature, only one specifically designed for detecting methanogenic archaea in stool samples, exhibited exceptional coverage levels for the domain and various archaea phyla. CONCLUSIONS: Our in silico analysis underscores the need for designing new primers targeting not only methanogenic archaea but also nanoarchaeal and thaumarchaeota groups to gain a comprehensive understanding of the archaeal oral community. By doing so, researchers can pave the way for further advancements in the field of oral archaeome research.

Eloísa Díaz en el Primer Congreso Latinoamericano de Medicina, Santiago 1901 / Eloísa Díaz in the First Latin American Congress of Medicine, Santiago 1901

Revisando el segundo volumen de actas del Primer Congreso Latinoamericano de Medicina, reunido en Santiago los nueve primeros días del siglo XX, encontramos una conferencia de Eloísa Díaz, primera mujer chilena y latinoamericana en graduarse de médico, pese a la discriminación sufrida, que la obligó incluso a asistir a clases acompañada por su madre. Se analiza tanto esta conferencia sobre la organización del servicio médico escolar, como la introducción a su tesis de grado, y de paso las presentaciones de otros médicos participantes, haciendo hincapié en la patología infecciosa discutida y en su lenguaje verboso y florido.

Analyzing the second volume of proceedings of the First Latin American Congress of Medicine held in Santiago on the first nine days of the twentieth century, we found a lecture by Eloísa Díaz, the first Chilean and Latin American woman to graduate as a doctor, despite the discrimination she suffered, which even forced her to attend classes accompanied by her mother. This conference on the school medical service is analyzed, as well as the introduction to her degree thesis, and incidentally the presentations of other participating doctors, emphasizing the infectious pathology discussed and its verbose and flowery language.

Novel microsatellite markers suggest significant genetic isolation in the Eastern Pacific sponge Aplysina gerardogreeni.

BACKGROUND: The Eastern Tropical Pacific (ETP) harbors a great diversity of Porifera. In particular, the Aplysina genus has acquired biotechnological and pharmacological importance. Nevertheless, the ecological aspects of their species and populations have been poorly studied. Aplysina gerardogreeni is the most conspicuous verongid sponge from the ETP, where it is usually found on rocky-coralline ecosystems. We evaluated the polymorphism levels of 18 microsatellites obtained from next-generation sequencing technologies. Furthermore, we tested the null hypothesis of panmixia in A. gerardogreeni population from two Mexican-Pacific localities. METHODS AND RESULTS: A total of 6,128,000 paired reads were processed of which primer sets of 18 microsatellites were designed. The loci were tested in 64 specimens from Mazatlan, Sinaloa (N = 32) and Isabel Island, Nayarit (N = 32). The microsatellites developed were moderately polymorphic with a range of alleles between 2 and 11, and Ho between 0.069 and 0.785. Fifteen loci displayed significant deviation from the Hardy-Weinberg equilibrium. No linkage disequilibrium was detected. A strong genetic structure was confirmed between localities using hierarchical Bayesian analyses, principal coordinates analyses, and fixation indices (FST = 0.108*). All the samples were assigned to their locality; however, there was a small sign of mixing between localities. CONCLUSIONS: Despite the moderate values of diversity in microsatellites, they showed a strong signal of genetic structure between populations. We suggest that these molecular markers can be a relevant tool to evaluate all populations across the ETP. In addition, 17 of these microsatellites were successfully amplified in the species A. fistularis and A. lacunosa, meaning they could also be applied in congeneric sponges from the Caribbean Sea. The use of these molecular markers in population genetic studies will allow assessment of the connectivity patterns in species of the Aplysina genus.

Primer registro de Tagetes imbricata (Asteraceae, Tageteae) en Perú / First record of Tagetes imbricata (Asteraceae, Tageteae) in Peru

Resumen Tagetes imbricata, una especie descrita recientemente para Argentina y Bolivia es registrada aquí por primera vez para Perú en base a colecciones de herbario y observaciones en campo disponibles en repositorios online de biodiversidad. Este nuevo registro expande significativamente el área de distribución de esta especie hacia el norte, abarcando en total más de 2000 kilómetros a lo largo de la cordillera de los Andes. Se presenta aquí por primera vez una ilustración para T. imbricata, detallando aspectos morfológicos. Se registran dos nombres vernáculos para esta especie: "kita-chick-chipa" y "chichijsa".

Abstract Tagetes imbricata, a species recently described from Argentina and Bolivia, is reported here for the first time from Peru based on herbaria collections and field observations available at biodiversity online repositories. This new record significantly expands the distribution area of this species to the north, covering a total of more than 2000 kilometers along the Andes Mountains. An illustration for T. imbricata is here provided for the first time, detailing morphological aspects. Two vernacular names, "kita-chick-chipa" and "chichijsa", are reported for this species.

Boletín epidemiológico semana 18: Unidad de análisis. Indicadores, Colombia, 2023 a primer trimestre 2024 / Epidemiological bulletin week 18: Unit of analysis. Indicators, Colombia, 2023 to first quarter 2024

La Unidad de análisis es el proceso metodológico que permite caracterizar los casos priorizados de eventos objeto de vigilancia en salud pública por medio del estudio de sus fuentes de información, para clasificarlos e identificar los factores asociados o causas relacionadas con su aparición. Bajo la metodología de análisis de problemas, se pueden identificar factores ambientales, sociales y geográficos que no son competencia directa o exclusiva del sector salud, por lo anterior, el resultado del tablero de problemas es un insumo para justificar el planteamiento de directrices y políticas públicas para hacer frente a los problemas de salud y sus determinantes sociales. Para 2023 se realizó la medición de los siguientes indicadores de gestión: cumplimiento en el cargue de unidades de análisis, oportunidad en el cargue de la unidad de análisis, oportunidad en el cargue del tablero de problemas; para ese año el cumplimiento correspondía al envío del tablero de problemas y en el 2024 se inició el cálculo a partir del número de tableros de problemas de los eventos priorizados cargados oportunamente al aplicativo unidades de análisis de casos especiales (UACE), utilizando como denominador el número de casos o muertes confirmadas por unidades de análisis que requieren tablero de problemas. Por último, a partir de 2023 se incluyó el indicador de proporción de unidades de análisis que cumplen con calidad en la metodología de realización, después de realizar una prueba piloto con todos los eventos durante el 2022 para su implementación.

The Unit of analysis is the methodological process that allows characterizing prioritized cases of events subject to public health surveillance by through the study of their sources of information, to classify them and identify the associated factors or causes related to their appearance. Under the problem analysis methodology, factors can be identified environmental, social and geographic issues that are not the direct or exclusive competence of the health sector; therefore, the result of the problem board is an input to justify the approach of guidelines and public policies to address health problems and their social determinants. For 2023, the following management indicators were measured: compliance in the loading of analysis units, timeliness in loading of the unit of analysis, opportunity in loading the problem board; For that year, compliance corresponded to the sending of the problem board and in 2024 the calculation began based on the number of problem boards of prioritized events loaded in a timely manner to the units application analysis of special cases (UACE), using as denominator the number of cases or deaths confirmed by analysis units that require problem dashboard. Finally, starting in 2023, the indicator was included of proportion of analysis units that comply with quality in the implementation methodology, after carrying out a pilot test with all the events during 2022 for implementation.

Extracción terapéutica de primeros molares Permanentes severamente destruidos en dentición mixta

En dentición mixta, el 1°Molar permanente es el más susceptible a caries, que desencadena un ciclo restaurador repetitivo y pérdida. El objetivo de este estudio fue evaluar factores determinantes de la extracción terapéutica de 1°Molares permanentes severamente destruidos en dentición mixta antes de la erupción del segundo molar permanente con un favorable cierre espontáneo del espacio residual. La metodología consistió en una revisión sistemática exploratoria en PubMed mediante búsqueda estratégica/específica. Incluyó diez artículos que abordaron factores como la edad cronológica ideal, etapa de desarrollo del segundo premolar y molar permanente, presencia del tercer molar, cierre espontáneo residual, pronóstico, y necesidad de tratamiento ortodóncico. En conclusión, la extracción terapéutica del 1°Molar antes de la erupción del segundo molar permanente está asociada con un favorable cierre espontáneo del espacio residual. Se evidencia mayor éxito con la presencia del tercer molar, segundo molar en etapa E y segundo premolar en etapa F (Demirjian).

Abstrato: Em dentição mista, o 1°molar permanente é o mais suscetível a cáries, desencadeando um ciclo restaurador repetitivo e perda. O objetivo deste estudo foi avaliar fatores determinantes da extração terapêutica de 1°molares permanentes severamente destruídos na dentição mista antes da erupção do segundo molar permanente com uma região espontânea favorável do espaço residual. A metodología utilizada no PubMed consistiu em uma revisão exploratória por meio de busca estratégica/específica. Foram incluídos dez artigos, abordando fatores como a idade cronológica ideal, estágio de desenvolvimento do segundo pré-molar e molar permanente, presença do terceiro molar, cierre espontâneo residual, pronóstico e necessidade de tratamento ortodôntico. Em conclusão, a extração terapêutica do 1°molar permanente antes da erupção do segundo molar permanente está associada a um fechamento espontâneo favorável do espaço residual. Maior sucesso está descrito quando na presença de terceiro molar, segundo molar no estágio E e segundo pré-molar no estágio F (Demirjian).

During mixed dentition, the 1st permanent molar is the most susceptible to caries, triggering a repetitive restorative cycle and loss. This study aimed to evaluate determinants of therapeutic extraction of severely damaged 1st permanent molars in mixed dentition before the eruption of the second permanent molar with favorable spontaneous closure of the residual space. The methodology involved a scoping review on PubMed using a specific search strategy. Ten articles were included addressing factors such as the ideal chronological age, stage of development of the second premolar and permanent molar, presence of the third molar, residual spontaneous closure, prognosis, and need for orthodontic treatment. In conclusion, therapeutic extraction of the 1st molar before the eruption of the second molar is associated with favorable spontaneous closure of the residual space. Greater success is evident with the presence of the third molar, the second molar in stage E, and the second premolar in stage F (Demirjian).

Envolvimento família-escola e suas implicações no desempenho escolar na educação básica / Compromiso familia-escuela y sus implicaciones en el rendimiento escolar en la educación primaria / The family-school engagement and its implications on student performance in basic education

RESUMO O estudo pretende problematizar como se estabelece o envolvimento família-escola visando à promoção dessa relação e do desempenho escolar dos alunos. Neste trabalho, analisou-se como pais e professores de quatro escolas públicas da cidade de Gravataí/RS percebem a si mesmos e ao outro frente a seu envolvimento escolar e a rotina compartilhada, e como essa relação pode favorecer o sucesso escolar de alunos do Ensino Fundamental I. Assim, foi realizado um estudo observacional comparativo, de caráter quantitativo e corte transversal, com 101 familiares e 12 professores de alunos em situação de sucesso escolar. Os resultados destacaram diferenças significativas em alguns domínios, evidenciando uma avaliação mais positiva dos professores a respeito de seu comprometimento com tal relação. Sugerem, especificamente nos domínios orientação e informação que a atitude dos pais e professores ao orientar, supervisionar e auxiliar um ao outro em suas funções e ao aluno pode favorecer um melhor desempenho acadêmico deste.

RESUMEN En este estudio se pretende problematizar cómo se establece el compromiso familia-escuela visando a la promoción de esa relación y del rendimiento escolar de los alumnos. En este estudio, se analizó cómo padres y profesores de cuatro escuelas públicas de la ciudad de Gravataí/RS perciben a sí mismos y al otro frente a su compromiso escolar y rutina compartida, y cómo esa relación puede favorecer el éxito escolar de alumnos de la Enseñanza Primaria I. Así, se realizó un estudio observacional comparativo, de carácter cuantitativo y corte transversal, con 101 familiares y 12 profesores de alumnos en situación de éxito escolar. Los resultados apuntan diferencias significativas en algunos dominios, evidenciando una evaluación más positiva de los profesores con respecto de su compromiso con tal relación. Sugieren, específicamente en los dominios orientacióneinformaciónque la actitud de los padres y profesores al orientar, supervisión y ayudar uno al otro en sus funciones y al alumno puede favorecer un mejor rendimiento académico de este.

ABSTRACT This study aims to discuss how the family-school involvement is established with a view to promoting this relation and students' academic performance. In this work, we analyzed how parents and teachers from four public schools in the city of Gravataí/RS perceive themselves and each other in relation to their school involvement and shared routine, and how this relation can favor the students' academic success in Elementary School I. Thus, a comparative observational study of a quantitative and cross-sectional nature was carried out with 101 family members and 12 teachers of students in a situation of academic success. The results highlighted significant differences in some domains, showing a more positive assessment by teachers regarding their commitment to this relation. They suggest, specifically in the guidance and information domains, that the parents and teachers' attitude when guiding, supervising and assisting each other in their roles and the student can lead to better academic performance.

Caracterización de los adultos mayores con cáncer colorrectal en la comunidad / Characterization of Community Older Adults with Colorectal Cancer

Introducción: El cáncer colorrectal es la neoplasia más frecuente del sistema digestivo en los adultos mayores. En Cuba es un problema de salud de primer orden por su elevada incidencia y mortalidad, que constituye la tercera causa de muerte. Objetivo: Caracterizar a los adultos mayores con cáncer colorrectal en el Policlínico Sur de Sancti Spíritus. Métodos: Investigación de tipo descriptiva en el Policlínico Sur del municipio y la provincia de Sancti Spíritus de enero a marzo del 2019. Muestra intencional de 127 adultos mayores, pertenecientes a consultorios urbanos. Las variables: edad, sexo, escolaridad, estadio del cáncer colorrectal al diagnóstico, estado de salud, comorbilidades, capacidad y percepción del autocuidado y supervivencia. Para determinar el estadio al diagnóstico se utilizó la clasificación anatomoclínica. La comorbilidad se midió mediante el índice de Chalson mientras que para medir la capacidad y percepción del autocuidado se empleó el Test de CYPAC-AM. Resultados: En la caracterización de la muestra predominaron las mujeres, el grupo de edad entre 70 y 79 años y la escolaridad de secundaria básica. El estadio II, con un mal estado de salud y la inadecuada percepción de autocuidado, fue mayoritario. La comorbilidad fue moderada con una supervivencia entre 40 a 60 meses. Conclusiones: La adecuada caracterización de los adultos mayores con cáncer colorrectal en la comunidad posibilita trazar estrategias dirigidas a la mejora del autocuidado y el estado de salud de los gerontes desde el primer nivel de atención(AU)

Introduction: Colorectal cancer is the most frequent neoplasm of the digestive system in older adults. In Cuba, it is a highly significant health problem due to its high incidence and mortality, also being the third cause of death. Objective: To characterize older adults with colorectal cancer at Policlínico Sur of Sancti Spíritus. Methods: A descriptive research was carried out in Policlínico Sur of Sancti Spíritus Municipality and Province from January to March 2019. The intentional sample was made up of 127 older adults, belonging to urban family medical offices. The variables were age, sex, school level, stage of colorectal cancer at diagnosis, health status, comorbidities, selfcare capacity and perception, and survival. The anatomoclinical classification was used to determine the stage at diagnosis. Comorbidity was measured using the Chalson index, while the CYPAC-AM (older adult selfcare capacity and perception) test was used to measure selfcare capacity and perception. Results: In the characterization of the sample, there was a predominance of women, the age group between 70 and 79 years, and the junior high school level. Stage II prevailed, together with poor health status and inadequate selfcare perception. Comorbidity was moderate, with survival between 40 to 60 months. Conclusions: The adequate characterization of older adults with colorectal cancer in the community makes it possible to outline strategies aimed at improving selfcare and their health status from the first level of care(AU)

LIMITATION OF PRIMERS USED IN PCR FOR THE CHARACTERIZATION OF LEISHMANIA INFANTUM.

Conventional PCR provides Leishmania species characterization with even a small amount of biological material. Species-specific primers have been a widely used alternative; however, nonspecific amplifications are a reality, interfering with PCR efficiency. In endemic areas with multiple etiological agents for leishmaniasis, there is a requirement for higher specificity of primers. This study evaluates 3 pairs of primers described for the identification and characterization of Leishmania infantum. Primers RV1/RV2, LEISH1/LEISH2, and FLC2/RLC2 were used with the DNA of L. infantum, Leishmania amazonensis, and Leishmania braziliensis. An initial temperature curve was performed (52-62 C) to determine the optimal annealing temperature, followed by a dilution curve of Leishmania DNA (500 pg/µl, 50 pg/µl, 5 pg/µl, 500 fg/µl, 50 fg/µl, 5 fg/µl, and 0.5 fg/µl) to be used for analytical sensitivity. RV1/RV2 PCR amplified L. infantum and L. amazonensis at all analyzed temperatures; LEISH1/LEISH2 PCR amplified all 3 species of Leishmania, although at some temperatures L. infantum was specifically amplified, and, finally, FLC2/RLC2 PCR amplified only L. infantum at all temperatures analyzed. In terms of sensitivity, RV1/RV2 PCR detected 1 fg of L. infantum DNA and 100 pg of L. amazonensis DNA; LEISH1/LEISH2 PCR detected 1 fg of L. infantum DNA, 100 fg of L. amazonensis DNA, and 10 fg of L. braziliensis DNA; and FLC2/RLC2 PCR detected 10 fg of L. infantum DNA. Thus, PCR with FLC2/RLC2 primers is best suited for the molecular characterization of L. infantum, especially in areas where there is an incidence of more than 1 Leishmania species, such as South America.

Analysis of the Internal and External Morphology of the Mandibular First Molar in a Chilean Sub-Population Using Cone-Beam Computed Tomography / Análisis de la Morfología Interna y Externa del Primer Molar Mandibular en una Subpoblación Chilena Mediante Tomografía Computarizada de Haz Cónico

SUMMARY: The mandibular first molar (MFM) commonly presents two roots with two canals in the mesial root and one or two canals in the distal root. However, morphological variations have been described in different populations, which must be considered when planning endodontic treatment. The aim of this study was to analyze the internal and external morphology of the MFM in a Chilean sub-population using cone-beam computed tomography (CBCT) images. An in vivo cross-sectional, descriptive, and observational study was conducted using CBCT exams from 351 right and left MFM. The data were analyzed by descriptive statistics using the Chi- Square test for categorical variables, Fisher's exact test, the Mann-Whitney U non-parametric test for two independent samples, and the Wilcoxon non-parametric test for related samples. Of the total sample, 1 root was observed in 2.27 % of the cases, 2 roots in 93.73 %, and 3 roots in 4 %. In relation to the number of canals, 71.23 % of the MFM showed 3 root canals, 16.81 % 4 canals, 9.69 % 2 canals, and 2.28 % 1 canal. Of all the studied cases, 2.3 % had a C-shaped anatomy. In terms of morphology, using Zhang's classification, variant 3 was observed in 71.23 %, variant 4 in 12.82 %, variant 1 in 9.67 %, variant 6 in 4 %, and variant 8 in 2.28 %. In conclusion, the morphology of the MFM is variable in a Chilean sub-population, and these variations must be considered before and during endodontic therapy. CBCT proved to be an effective tool for the in vivo study of tooth morphology.

El primer molar mandibular (MFM) comúnmente presenta dos raíces con dos canales en la raíz mesial y uno o dos canales en la raíz distal. Sin embargo, se han descrito variaciones morfológicas en distintas poblaciones, las que se deben tener en consideración al momento de planificar el tratamiento endodóntico. El objetivo de este estudio fue analizar la morfología interna y externa del MFM en una sub población chilena mediante el uso de imágenes de tomografía computarizada Cone Beam (CBCT). Se realizó un estudio transversal, descriptivo y observacional in vivo empleando exámenes CBCT de 351 MFM tanto derechos como izquierdos. Los datos se analizaron mediante estadística descriptiva empleando la prueba Chi-Cuadrado para variables categóricas, el test exacto de Fisher, la prueba no paramétrica de U-Mann-Whitney para dos muestras independientes y la prueba no paramétrica de Wilcoxon para muestras relacionadas. Del total de la muestra se observó 1 raíz en un 2.27 % de los casos, 2 raíces en 93.73 % y 3 raíces en un 4 %. En relación al número de canales un 71.23 % de los MFM mostraron 3 canales radiculares, un 16.81 % 4 canales, un 9.69 % 2 canales y un 2.28 % 1 canal. Del total de los casos estudiados un 2.3 % se presentó anatomía en forma de C. En relación a la morfología, empleando la clasificación de Zhang, se observó en un 71.23 % la variante tipo 3, en un 12.82 % la variante tipo 4, en un 9.67 % la variante tipo 1, en un 4 % variante tipo 6 y en un 2,28 % variante tipo 8. En conclusión, la morfología del MFM es variable en una subpoblación chilena y estas variaciones deben ser consideradas antes y durante la terapia endodóntica. El CBCT demostró ser una herramienta eficaz para el estudio in vivo de la morfología dentaria.

O que os/as calouros/as ensinam para a Universidade?: Uma reflexão / What do freshmen teach to the university?: a reflection / ¿Qué enseñan los estudiantes de primer año a la Universidad?: una reflexión

Partindo da perspectiva da psicologia escolar, esse artigo focaliza a condição de calouro/a como analisador importante do processo educativo que acontece na instituição universitária, a qual denuncia e anuncia condições e possibilidades que limitam e ampliam a potência criadora de seus membros. Ao longo do artigo, buscamos superar o paradigma da adaptação, que exige do/a recém-ingressante a assimilação de normas e regras institucionais a fim de adquirir status de membro. Como contraponto, pensamos a participação de todos os membros da comunidade acadêmica na recepção aos/às calouros/as e construção de uma cultura institucional dinâmica e criadora de novos viveres e fazeres universitários, acolhedores da sua diversidade. Discutimos, ainda, a presença do/a calouro/a como oportunidade de um segundo olhar da psicologia escolar sobre as relações interpessoais, as práticas e os processos institucionais e pedagógicos.

This theoretical study proposes to think about the body from the plurality and the affections' perspectives, in an attempt to understand how the psychology works, on the virtual modality, reaches corporeity and the expansion of its power to exist. These questions were raised from a Group for Listening and Welcoming Services o the virtual modality. The theoretical perspective starts from Spinoza's conception of the affections, articulated with the notion of self-care. The loneliness reported on the group was our analyzer, in an attempt to reflect about the encounter's weakening. This led us to reflect about the impacts of the body's loss on the online presence, the (im)possibility of encountering the difference and the role of virtuality in the liberal economic model applied to psychology. It is necessary to cover the possibilities of action that do not abstain from present time demands, but also to recognize the dangers of capture devices. (AU)

Este ensayo teórico propone pensar el cuerpo desde la perspectiva de las afecciones, intentando comprender cómo el trabajo de la psicología en la modalidad a distancia alcanza la corporeidad y su potencia de existir. Tales interrogantes surgieron en la experiencia de un Grupo de Escucha y Acogida en la modalidad a distancia. Orientó el trabajo la concepción Spinozista de los afectos, articulada con la noción de cuidado de sí. La soledad relatada en los grupos fue el analizador para reflexionar sobre la producción del encuentro y nos llevó a pensar en los impactos de la pérdida del cuerpo en la presencia online, la (im)posibilidad de encontrar la diferencia y a reflexionamos sobre la virtualidad en el modelo económico liberal aplicado a la psicología. Es necesario abarcar las posibilidades de acción que no se abstengan de las exigencias de hoy, pero que también reconozcan los peligros de los dispositivos de captura. (AU)

Is structural connectivity different in child and adolescent relatives of patients with bipolar disorder? A narrative review according to studies with DTI.

INTRODUCTION: Bipolar disorder (BD) has been associated with a decrease in white matter integrity. Diffusion tensor imaging (DTI) studies have enabled these changes to be elucidated with higher quality. Due to BD's high heritability, some studies have been conducted in relatives of BD patients looking at white matter integrity, and have found that structural connectivity may also be affected. This alteration has been proposed as a potential BD biomarker of vulnerability. However, there are few studies in children and adolescents. OBJECTIVE: To conduct a review of the literature on changes in white matter integrity determined by DTI in high-risk children and adolescents. RESULTS: Brain structural connectivity in the paediatric population is described in studies using DTI. Changes in the myelination process from its evolution within normal neurodevelopment to the findings in fractional anisotropy (FA) in BD patients and their high-risk relatives are also described. CONCLUSIONS: Studies show that both BD patients and their at-risk relatives present a decrease in FA in specific brain regions. Studies in children and adolescents with a high risk of BD, indicate a reduced FA in axonal tracts involved in emotional and cognitive functions. Decreased FA can be considered as a vulnerability biomarker for BD.

¿Es diferente la conectividad estructural en niños y adolescentes familiares de pacientes con trastorno bipolar? Una revisión narrativa según estudios con DTI

Introducción: El trastorno afectivo bipolar (TAB) se ha asociado con una disminución de la integridad de la sustancia blanca. Los estudios con imágenes con tensor de difusión (DTI) han permitido elucidar con una mayor calidad estos cambios. Debido a la gran heredabilidad del TAB, se han realizado estudios en familiares de pacientes con TAB acerca de la integridad de la sustancia blanca, y se ha encontrado que la conectividad estructural también puede estar afectada. Dicha alteración se ha propuesto como un potencial biomarcador de vulnerabilidad a este trastorno. Sin embargo, los estudios en niños y adolescentes son pocos. Objetivo: Revisar la literatura sobre los cambios en la integridad de la sustancia blanca determinados mediante DTI en niños y adolescentes con alto riesgo. Resultados: Se describe la conectividad estructural cerebral en la población pediátrica en estudios que utilizaron DTI. Se describen los cambios en el proceso de mielinización desde su evolución dentro del neurodesarrollo normal hasta los hallazgos en la anisotropía fraccional (AF) en pacientes con TAB y los familiares en alto riesgo. Conclusiones: Los estudios demuestran que tanto pacientes con TAB como sus familiares en riesgo presentan disminución de la AF en regiones cerebrales específicas. Los estudios en niños y adolescentes con riesgo familiar de TAB señalan una AF reducida en tractos axonales implicados en funciones emocionales y cognitivas. La disminución de la AF puede considerarse como un biomarcador de vulnerabilidad al TAB.

Introduction: Bipolar disorder (BD) has been associated with a decrease in white matter integrity. Diffusion tensor imaging (DTI) studies have enabled these changes to be elucidated with higher quality. Due to BD's high heritability, some studies have been conducted in relatives of BD patients looking at white matter integrity, and have found that structural connectivity may also be affected. This alteration has been proposed as a potential BD biomarker of vulnerability. However, there are few studies in children and adolescents. Objective: To conduct a review of the literature on changes in white matter integrity determined by DTI in high-risk children and adolescents. Results: Brain structural connectivity in the paediatric population is described in studies using DTI. Changes in the myelination process from its evolution within normal neurodevelopment to the findings in fractional anisotropy (FA) in BD patients and their high-risk relatives are also described. Conclusions: Studies show that both BD patients and their at-risk relatives present a decrease in FA in specific brain regions. Studies in children and adolescents with a high risk of BD, indicate a reduced FA in axonal tracts involved in emotional and cognitive functions. Decreased FA can be considered as a vulnerability biomarker for BD.