Phaseolotoxin: Environmental Conditions and Regulatory Mechanisms Involved in Its Synthesis.

Phaseolotoxin is an antimetabolite toxin produced by diverse pathovars of Pseudomonas syringae which affects various plants, causing diseases of economic importance. Phaseolotoxin contributes to the systemic dissemination of the pathogen in the plant, therefore it is recognized as a major virulence factor. Genetic traits such as the Pht cluster, appear defining to the toxigenic strains phaseolotoxin producers. Extensive research has contributed to our knowledge concerning the regulation of phaseolotoxin revealing a complex regulatory network that involves processes at the transcriptional and posttranscriptional levels, in which specific and global regulators participate. Even more, significant advances in understanding how specific signals, including host metabolites, nutrient sources, and physical parameters such as the temperature, can affect phaseolotoxin production have been made. A general overview of the phaseolotoxin regulation, focusing on the chemical and physical cues, and regulatory pathways involved in the expression of this major virulence factor will be given in the present work.

Cyanopeptides occurrence and diversity in a Brazilian tropical reservoir: Exploring relationships with water quality.

Microcystins (MCs) are a class of toxic secondary metabolites produced by some cyanobacteria strains that endanger aquatic and terrestrial organisms in various freshwater systems. Although patterns in MC occurrence are being recognized, divergences in the global data still hamper our ability to predict the toxicity of cyanobacterial blooms. This study aimed (i) to determine the dynamics of MCs and other cyanopeptides in a tropical reservoir, (ii) to investigate the correlation between peptides and potential cyanotoxin producers (iii) identifying the possible abiotic factors that influence the peptides. We analyzed, monthly, eight MC variants (MC-RR, -LA, -LF, -LR, -LW, -YR, [D-Asp3]-RR and [D-Asp3]-LR) and other peptides in 47 water samples collected monthly, all season long, from two sampling sites in a tropical eutrophic freshwater reservoir, in southeastern Brazil. The cyanopeptides were assessed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The biomass of potential cyanobacterial producers and water quality variables were measured. MCs were detected in both sampling sites year-round; the total MC concentration varied from 0.21 to 4.04 µg L-1, and three MC variants were identified and quantified (MC-RR, [D-Asp3]-RR, -LR). Additionally, we identified 28 compounds belonging to three other cyanopeptide classes: aeruginosin, microginin, and cyanopeptolin. As potential MC producers, Microcystis spp. and Dolichospermum circinalis were dominant during the study, representing up to 75% of the total phytoplankton. Correlational and redundancy analysis suggested positive effects of dissolved oxygen, nitrate, and total phosphorus on MC and microginins concentration, while water temperature appeared to favor aeruginosins. A comparison between our results and historical data showed a reduction in total phosphorus and cyanobacteria, suggesting increased water quality in the reservoir. However, the current MC concentrations indicate a rise in cyanobacterial toxicity over the last eight years. Moreover, our study underscores the pressing need to explore cyanopeptides other than MCs in tropical aquatic systems.

Local and Regional Dynamics of Native Maize Seed Lot Use by Small-Scale Producers and Their Impact on Transgene Presence in Three Mexican States.

Mexico harbors over 50% of maize's genetic diversity in the Americas. Native maize varieties are actively managed by small-scale producers within a diverse array of cultivation systems. Seed lot use, exchange and admixture has consequences for the in situ conservation of such varieties. Here we analyze native maize seed management dynamics from 906 small-scale producers surveyed in three Mexican states: Mexico City, Oaxaca and Chiapas. Furthermore, we analyze how their management practices can relate to transgene presence, which was experimentally documented for maize samples associated with the applied surveys. Through a data mining approach, we investigated which practices might be related with a higher probability of transgene presence. The variables found to have a strong spatial association with transgene presence were: for Mexico City, maize producers with larger parcels; for Oaxaca, producer's age (43-46 years) and the sale of seed; for Chiapas, the use of agricultural machinery and younger producers (37-43 years). Additionally, transgene presence and frequency within the socioeconomic regions of Oaxaca and Chiapas was analyzed. In Oaxaca, higher transgene frequencies occurred in regions where transgene presence had been previously reported. In Chiapas, the border regions with Guatemala as well as a region where reproduction of improved seed takes place, the highest proportion of positive samples were found. A detailed mapping of regional seed markets and seed exchange sites together with deployment of national and local biosecurity measures, could help prevent the further spread of transgenes into native maize varieties, as well as improve conservation efforts.

An overview of carbapenem-resistant organisms from food-producing animals, seafood, aquaculture, companion animals, and wildlife.

Carbapenem resistance (CR) is a major global health concern. CR is a growing challenge in clinical settings due to its rapid dissemination and low treatment options. The characterization of its molecular mechanisms and epidemiology are highly studied. Nevertheless, little is known about the spread of CR in food-producing animals, seafood, aquaculture, wildlife, their environment, or the health risks associated with CR in humans. In this review, we discuss the detection of carbapenem-resistant organisms and their mechanisms of action in pigs, cattle, poultry, seafood products, companion animals, and wildlife. We also pointed out the One Health approach as a strategy to attempt the emergency and dispersion of carbapenem-resistance in this sector and to determine the role of carbapenem-producing bacteria in animals among human public health risk. A higher occurrence of carbapenem enzymes in poultry and swine has been previously reported. Studies related to poultry have highlighted P. mirabilis, E. coli, and K. pneumoniae as NDM-5- and NDM-1-producing bacteria, which lead to carbapenem resistance. OXA-181, IMP-27, and VIM-1 have also been detected in pigs. Carbapenem resistance is rare in cattle. However, OXA- and NDM-producing bacteria, mainly E. coli and A. baumannii, are cattle's leading causes of carbapenem resistance. A high prevalence of carbapenem enzymes has been reported in wildlife and companion animals, suggesting their role in the cross-species transmission of carbapenem-resistant genes. Antibiotic-resistant organisms in aquatic environments should be considered because they may act as reservoirs for carbapenem-resistant genes. It is urgent to implement the One Health approach worldwide to make an effort to contain the dissemination of carbapenem resistance.

Artisanal Goat Coalho Cheese with Cachaça as Flavoring Agent.

Cachaça is a traditional Brazilian drink that has the potential to offer a new sensory and technological strategy for artisanal cheesemaking, particularly for small-scale producers and family farming. This study aimed to investigate the effects of cachaça immersion on the physicochemical, microbiological, color, texture, and sensory parameters of artisanal goat coalho cheeses using three different varieties of cachaça immersion. The results showed that cachaça immersion did not affect the cheese's proximate composition or starter culture viability, indicating its suitability as a new method for artisanal cheese production. Additionally, gold cachaça aged in oak casks was the most effective variety for sensory acceptance and purchase intention, suggesting that it may be a valuable strategy for small-scale producers to add value and encourage the consumption of artisanal goat coalho cheeses without compromising their quality. Thus, this study provides important insights for small-scale producers and family farming to enhance their product offerings and increase their competitiveness in the market.

Análisis de la miel de abeja en Chile: un estudio de caso en el sector apícola / Honey bee analysis in Chile: a case study in the beekeeping sector / Análise das abelhas no Chile: estudo de caso no setor da apicultura

RESUMEN La finalidad de este estudio consiste en analizar la miel de abeja en Chile para proponer lineamientos estratégicos que permitan contribuir a la gestión del sector apícola chileno. Para ello, la metodología utilizada es un estudio de caso, para lo cual se aplica en el año 2021 una encuesta a 84 consumidores y a 40 apicultores de la Región del Biobío en Chile. Los resultados del estudio permiten apreciar que el 38% de los apicultores se dedica hace más de 10 años a la producción de miel y el 50% senala que aprendió solo del rubro, siendo las principales dificultades enfrentadas la organización y el tiempo, la sanidad y nutrición. Además, el 42% de los apicultores plantean que el cambio climático, las plagas y sequías afectaron la producción de la miel entre un 0 y 20%. Mientras que el 30% de los consumidores encuestados considera como prioridad el prestigio o reputación del producto. Se concluye la relevancia de los lineamientos estratégicos para guiar el desarrollo de la cadena de la miel en Chile y se recomienda su implementación para el fortalecimiento del sector apícola a nivel nacional e internacional. СLASIFICACIÓN JEL: 170, 013

ABSTRACT The purpose of this study is to analyze bee honey in Chile to propose strategic guidelines that allow contributing to the management of the Chilean beekeeping sector For this, the methodology used is a case study for which a survey of 84 consumers and 40 beekeepers of the Biobío Region in Chile is applied in 2021. The results of the study allow us to appreciate that 38% of beekeepers have been dedicated to honey production for more than 10 years and 50% indicate that they only learned about the field, the main difficulties faced being organization and time, health and nutrition. In addition, 42% of beekeepers indicate that climate change, plagues and droughts affected honey production between 0 and 20%. While 30% of consumers surveyed consider the prestige or reputation of the product as a priority. The relevance of the strategic guidelines to guide the development of the honey chain in Chile is concluded and its implementation is recommended for the strengthening of the beekeeping sector at a national and international level. JEL CLASSIFICATION: 170, 013

RESUMO O objetivo deste estudo é analisar a análise das abelhas no Chile a fim de propor diretrizes estratégicas que contribuam para a gestão do setor apícola chileno. Para este fim, a metodologia utilizada é um estudo de caso, para o qual foi realizada uma pesquisa com 84 consumidores e 40 apicultores na Região de Biobío, no Chile, em 2021. Os resultados do estudo mostram que 38% dos apicultores estão envolvidos na produção de mel há mais de 10 anos e 50% dizem que só aprenderam sobre o negócio, sendo as principais dificuldades a organização e o tempo, a saúde e a nutrição. Além disso, 42% dos apicultores dizem que a mudança climática, as pragas e as secas afetaram a produção de mel em 0-20%. Enquanto 30% dos consumidores pesquisados consideram o prestígio ou a reputação do produto como prioridade. A relevância das diretrizes estratégicas para orientar o desenvolvimento da cadeia do mel no Chile está concluída e sua implementação é recomendada para o fortalecimento do setor apícola a nível nacional e internacional. CLASSIFICAÇÃO JEL: 170, 013

Roadmapping 5.0 Technologies in Agriculture: A Technological Proposal for Developing the Coffee Plant Centered on Indigenous Producers' Requirements from Mexico, via Knowledge Management.

The coffee plant, with more than 40 billion shrubs, 9 million tons of grains produced, and 80% of its production accounted for by small-scale producers, has been severely damaged since the emergence of Hemileia vastatrix and Hypothenemus hampei. Despite technological support, these pests have caused 20% to 40% production losses, a 50% to 60% deficit in performance, and a cost of between USD 70 million and USD 220 million to the world economies, which forces us to rethink actions centered on people as the key elements to develop appropriate solutions. For this, the present study presents a technological proposal centered on small indigenous coffee producer requirements for introducing Industry 5.0 technologies, considering roadmapping, knowledge management, statistical analysis, and the social, productive, and digital contexts of five localities in Mexico. The results show a correlation between monitoring and control, soil analysis, the creation of organic fertilizers, accompaniment, and coffee experimentation, as the actions to be implemented, proposing the introduction of a mobile application; sensors, virtual platforms, dome-shaped greenhouses, and spectrophotometric technology as relevant technologies centered on indigenous coffee producers' requirements. This study is important for policymakers, academics, and producers who wish to develop strategies centered on people in Mexico and the world.

Process intensification for the production of yellow fever virus-like particles as potential recombinant vaccine antigen.

Yellow fever (YF) is a life-threatening viral disease endemic in parts of Africa and Latin America. Although there is a very efficacious vaccine since the 1930s, YF still causes 29,000-60,000 annual deaths. During recent YF outbreaks there were issues of vaccine shortage of the current egg-derived vaccine; rare but fatal vaccine adverse effects occurred; and cases were imported to Asia, where the circulating mosquito vector could potentially start local transmission. Here we investigated the production of YF virus-like particles (VLPs) using stably transfected HEK293 cells. Process intensification was achieved by combining sequential FACS (fluorescence-activated cell sorting) rounds to enrich the stable cell pool in terms of high producers and the use of perfusion processes. At shaken-tube scale, FACS enrichment of cells allowed doubling VLP production, and pseudoperfusion cultivation (with daily medium exchange) further increased VLP production by 9.3-fold as compared to batch operation mode. At perfusion bioreactor scale, the use of an inclined settler as cell retention device showed operational advantages over an ATF system. A one-step steric exclusion chromatography purification allowed significant removal of impurities and is a promising technique for future integration of upstream and downstream operations. Characterization by different techniques confirmed the identity and 3D-structure of the purified VLPs.

Tracing a path for opium gum from Mexico as a safe supply harm reduction measure for Canada.

Historically, the poppy plant has had multiple uses including as a food product and with medical uses in pain relief; today it is most commonly known as the plant from which heroin is derived. The United Nations international drug control regime currently only allows legal poppy production for medical use in 19 countries. Although Mexico is the third largest illegal poppy producer in the world, no Latin American country is currently allowed to legally produce poppies. Meanwhile, the United States and Canada are experiencing an overdose crisis due in large part to the adulteration and substitution of heroin with fentanyl and its analogues, while the price of opium gum has dropped 80% in the last two years in poppy producing areas of Mexico. The prohibition of opium has wide ranging health and development impacts that bring up a moral imperative regarding the safe supply of diverse opium-based products -including opium gum and heroin- that urgently need to be explored and addressed. Opium gum can be used orally or smoked, reducing riskier modes of administration and possible fatal overdoses. This article discusses the political and legal possibilities of safely supplying opium gum and manually extracted heroin from Mexico to Canada as a public health, harm reduction and development policy.

Prevalência de mastite bovina no planalto de Santa Catarina / Bovine mastitis prevalence in the plateau of Santa Catarina

A mastite bovina é amplamente distribuída nos rebanhos leiteiros, acarretando em perdas econômicas aos produtores. Assim, o objetivo do estudo foi identificar a prevalência de mastite clínica e subclínica nos rebanhos de bovinos leiteiros, na região do Planalto Catarinense. Foram visitadas 44 propriedades leiteiras, onde foram registradas as principais informações sobre o rebanho de fêmeas em lactação. O diagnóstico de mastite clínica foi realizado pelo teste da caneca de fundo preto, enquanto, para mastite subclínica, foi utilizado o CMT (California Mastitis Test). Os dados foram processados no software estatístico R, para cálculo da prevalência, e foi aplicado teste Qui-quadrado ao nível de 5% de significância para as seguintes variáveis: manejos de ordenha, dados individuais dos animais (número e estágio de lactação) e Escore de Limpeza de Úbere (ELU). Foram estimadas, a partir do CMT, as perdas produtivas decorrentes da mastite subclínica. A prevalência média de mastite clínica e subclínica foi, respectivamente, de 4,29% e 76,92% para os animais avaliados. Não foram encontradas associações entre a mastite e o manejo de ordenha, os dados individuais dos animais e o ELU. As perdas produtivas são importantes, com mais de 15.000 litros de leite que deixam de ser produzidos por mês, devido à mastite subclínica no rebanho. Assim, as prevalências encontradas são preocupantes, visto a importância da atividade como fonte de renda aos produtores. Órgãos públicos devem investir na conscientização e em medidas de controle e prevenção da doença para melhorar a rentabilidade desses produtores e para que a atividade tenha crescimento na região.(AU)

Bovine mastitis is widely distributed among dairy herds and causes large economic losses to farmers. The objective of this study was to identify the prevalence of clinical and subclinical mastitis in dairy herds in the plateau of Santa Catarina. Forty-four dairy farms were visited and the main information about the lactating female herd was recorded. The test of the mug of black background was performed for the identification of clinical mastitis, while CMT (California Mastitis Test) was performed for subclinical mastitis. The data was processed with the statistical program R, where the prevalence was calculated. Chi-square test at 5% significance level were used to the variables: milking maneuvers, individual animal data (number and stage of lactation) and Udder Cleaning Score (ELU). The production losses resulting from subclinical mastitis were estimated through the CMT result. The mean prevalence of clinical and subclinical mastitis was 4.29% and 78,92%, respectively, for the animals evaluated. There was no correlation between the prevalence found and the management of milking, number and stage of lactation and ELU. Production losses were significant, with more than 15.000 liters of milk being stopped every month, due to the presence of subclinical mastitis in the herd. Thus, the prevalence found is worrisome, given the importance of the activity as a source of income for these producers. Public bodies should invest in awareness and in measures of control and prevention of the disease so that the profitability of these producers improves and the activity grows in the region.(AU)

Prevalência de mastite bovina no planalto de Santa Catarina / Bovine mastitis prevalence in the plateau of Santa Catarina

A mastite bovina é amplamente distribuída nos rebanhos leiteiros, acarretando em perdas econômicas aos produtores. Assim, o objetivo do estudo foi identificar a prevalência de mastite clínica e subclínica nos rebanhos de bovinos leiteiros, na região do Planalto Catarinense. Foram visitadas 44 propriedades leiteiras, onde foram registradas as principais informações sobre o rebanho de fêmeas em lactação. O diagnóstico de mastite clínica foi realizado pelo teste da caneca de fundo preto, enquanto, para mastite subclínica, foi utilizado o CMT (California Mastitis Test). Os dados foram processados no software estatístico R, para cálculo da prevalência, e foi aplicado teste Qui-quadrado ao nível de 5% de significância para as seguintes variáveis: manejos de ordenha, dados individuais dos animais (número e estágio de lactação) e Escore de Limpeza de Úbere (ELU). Foram estimadas, a partir do CMT, as perdas produtivas decorrentes da mastite subclínica. A prevalência média de mastite clínica e subclínica foi, respectivamente, de 4,29% e 76,92% para os animais avaliados. Não foram encontradas associações entre a mastite e o manejo de ordenha, os dados individuais dos animais e o ELU. As perdas produtivas são importantes, com mais de 15.000 litros de leite que deixam de ser produzidos por mês, devido à mastite subclínica no rebanho. Assim, as prevalências encontradas são preocupantes, visto a importância da atividade como fonte de renda aos produtores. Órgãos públicos devem investir na conscientização e em medidas de controle e prevenção da doença para melhorar a rentabilidade desses produtores e para que a atividade tenha crescimento na região.

Bovine mastitis is widely distributed among dairy herds and causes large economic losses to farmers. The objective of this study was to identify the prevalence of clinical and subclinical mastitis in dairy herds in the plateau of Santa Catarina. Forty-four dairy farms were visited and the main information about the lactating female herd was recorded. The test of the mug of black background was performed for the identification of clinical mastitis, while CMT (California Mastitis Test) was performed for subclinical mastitis. The data was processed with the statistical program R, where the prevalence was calculated. Chi-square test at 5% significance level were used to the variables: milking maneuvers, individual animal data (number and stage of lactation) and Udder Cleaning Score (ELU). The production losses resulting from subclinical mastitis were estimated through the CMT result. The mean prevalence of clinical and subclinical mastitis was 4.29% and 78,92%, respectively, for the animals evaluated. There was no correlation between the prevalence found and the management of milking, number and stage of lactation and ELU. Production losses were significant, with more than 15.000 liters of milk being stopped every month, due to the presence of subclinical mastitis in the herd. Thus, the prevalence found is worrisome, given the importance of the activity as a source of income for these producers. Public bodies should invest in awareness and in measures of control and prevention of the disease so that the profitability of these producers improves and the activity grows in the region.

Dynamics of Operation and Identification of Actions to Improve the Peasant Market in the Municipality of Florence: the Case of COOPMERCASAN / Dinámica del funcionamiento y retos del mercado campesino coopmercasan de florencia (caquetá)

RESUMEN El mercado campesino de Coopmercasan inició su funcionamiento en 2011 brindando productos agrícolas frescos e inocuos al municipio de Florencia, departamento del Caquetá. En ocho años de operación, han tenido aciertos y desaciertos, también han recibido el apoyo de diferentes instituciones, sin embargo, a la fecha se desconoce sus realidades actuales y los retos y desafíos que han venido cambiando a lo largo del tiempo. Por lo anterior, esta investigación buscó construir las dinámicas de esta experiencia a través de la autorreflexión, la información base para la toma de decisiones y la orientación de sus acciones de mejora. El trabajo se realizó en el municipio de Florencia, las herramientas para el levantamiento de la información empleadas fueron encuestas, entrevistas y observación participante. Dentro de los principales hallazgos se tiene que, si bien el mercado ha logrado posicionarse con una base interesante de clientes, su oferta no está cumpliendo las expectativas respecto de los volúmenes y la diversidad de demandada de los clientes, y aunque la gran mayoría de los apoyos entregados por las instituciones han sido dirigidos a procesos de fortalecimiento organizativos, sigue siendo el aspecto más débil.

ABSTRACT The COOPMERCASAN peasant market began its operation in 2011, providing fresh and safe agricultural products to the population of Florence. In these eight years of operation, they have had successes and failures, they have also received the support of different institutions, however, to date, their current realities are unknown, as well as the challenges, which have been changing over time. It is because of the above that this research sought to build the dynamics of this experience through self-reflection, base information for decision-making, and the orientation of their improvement actions. The work was carried out in the municipality of Florence, the tools for gathering the information were surveys, interviews, and participant observation. Among the main findings, it was discovered that, although the market has managed to position itself with an interesting customer base, its offer is not meeting expectations in terms of the volumes and diversity demanded by customers and that, although the vast majority of the support provided by the institutions has been directed towards organizational strengthening processes, it continues to be the weakest aspect.

Diagnóstico da produção de leite no município de Pejuçara-RS / Diagnosis of milk production in the municipality of Pejuçara-RS

A pesquisa teve por objetivo analisar a produção de leite bovino em diferentes níveis produtivos no município de Pejuçara-RS. Utilizou-se a metodologia de amostragem semiestratificada, não probabilística e de população finita. A pesquisa foi realizada com 35 produtores (90% de intervalo de confiança e 10% de margem de erro). As propriedades foram agrupadas em três estratos de produção: de 0 a 200 litros/dia; de 201 a 500 litros/dia; e acima de 501 litros/dia. A maioria dos produtores de leite possuem idade superior a 50 anos e mais de 20 anos de experiência nessa atividade. Do total apenas 7 produtores possuíam menos de 40 anos de idade. Os sistemas de produção são semi-intensivos, onde os animais são criados em pastagem cultivada e recebem ração e silagem de milho após as ordenhas. Esse sistema, atrelados a boa genética dos animais e o adequado manejo sanitário, promove uma adequada produção de leite animal/dia. Grande parte da renda do município de Pejuçara é oriunda da atividade leiteira, com isso o trabalho de extensão serve de apoio na tomada de decisão visando melhorias nos pontos críticos e o aprimoramento nos pontos positivos nas diversas propriedades do município.(AU)

The research had the objective of analyzing the production of bovine milk at different productive levels in the municipality of Pejuçara-RS. The semi-standardized, non-probabilistic and finite population sampling methodology was used. The research was carried out with 35 producers (90% confidence interval and 10% margin of error). The properties were grouped into three production strata: 0 to 200 liters / day; From 201 to 500 liters / day; And above 501 liters / day. Most dairy farmers are over 50 years old and over 20 years of experience in this activity. Of the total only 7 producers were under 40 years of age. The production systems are semi-intensive, where the animals are raised in cultivated pasture and receive feed and corn silage after the milking. This system, coupled with good animal genetics and adequate sanitary management, promotes an adequate production of animal milk / day. Much of the income of the municipality of Pejuçara is derived from the dairy activity, so the extension work serves as a support in the decision making aiming at improvements in the critical points and the improvement in the positive points in the diverse properties of the municipality.(AU)

Diagnóstico da produção de leite no município de Pejuçara-RS / Diagnosis of milk production in the municipality of Pejuçara-RS

A pesquisa teve por objetivo analisar a produção de leite bovino em diferentes níveis produtivos no município de Pejuçara-RS. Utilizou-se a metodologia de amostragem semiestratificada, não probabilística e de população finita. A pesquisa foi realizada com 35 produtores (90% de intervalo de confiança e 10% de margem de erro). As propriedades foram agrupadas em três estratos de produção: de 0 a 200 litros/dia; de 201 a 500 litros/dia; e acima de 501 litros/dia. A maioria dos produtores de leite possuem idade superior a 50 anos e mais de 20 anos de experiência nessa atividade. Do total apenas 7 produtores possuíam menos de 40 anos de idade. Os sistemas de produção são semi-intensivos, onde os animais são criados em pastagem cultivada e recebem ração e silagem de milho após as ordenhas. Esse sistema, atrelados a boa genética dos animais e o adequado manejo sanitário, promove uma adequada produção de leite animal/dia. Grande parte da renda do município de Pejuçara é oriunda da atividade leiteira, com isso o trabalho de extensão serve de apoio na tomada de decisão visando melhorias nos pontos críticos e o aprimoramento nos pontos positivos nas diversas propriedades do município.

The research had the objective of analyzing the production of bovine milk at different productive levels in the municipality of Pejuçara-RS. The semi-standardized, non-probabilistic and finite population sampling methodology was used. The research was carried out with 35 producers (90% confidence interval and 10% margin of error). The properties were grouped into three production strata: 0 to 200 liters / day; From 201 to 500 liters / day; And above 501 liters / day. Most dairy farmers are over 50 years old and over 20 years of experience in this activity. Of the total only 7 producers were under 40 years of age. The production systems are semi-intensive, where the animals are raised in cultivated pasture and receive feed and corn silage after the milking. This system, coupled with good animal genetics and adequate sanitary management, promotes an adequate production of animal milk / day. Much of the income of the municipality of Pejuçara is derived from the dairy activity, so the extension work serves as a support in the decision making aiming at improvements in the critical points and the improvement in the positive points in the diverse properties of the municipality.

Assessing Financial Impacts of Subclinical Mastitis on Colombian Dairy Farms.

Bovine mastitis is a dairy cattle disease with high economic impact. Subclinical mastitis (SCM) contributes to most of the financial losses. Colombia dairy sector accounts for 2.3% of the gross domestic product (GDP) and 24.3% of the livestock GDP. Milk production reaches 6,500 million liters/year from nearly 500,000 cattle farms and is mainly based on small-scale production systems. This study evaluates the financial impact of SCM and the potential for its control in three dairy farm strata in a region in Colombia. The objectives of the study were 1) to determine the perception of farmers about the SCM problem on their farms, 2) to assess prevalence and financial impact of SCM on farms and in the "Area five" sanitary region of the Bogota plateau, and 3) to assess costs and effectiveness of control methods of SCM. Information about disease management and decision-making process was obtained through a participatory epidemiology workshop and applying a semi-structured survey. A two-stage stratified cross sectional epidemiological study was conducted on dairy cattle from a region with approximately 400 farms and 12,000 cows, with a sample size of 55 farms. Prevalence of SCM was calculated by defining a cow as positive for the disease when any quarter had a somatic cell count (SCC) higher than 250 × 103 cells/ml. The prevalence of SCM in cows was 55.2%; significant differences were found between strata. Assessment of the financial impact of SCM in terms of milk losses was conducted using spreadsheet models. Milk production losses per farm ranged from 1.3% to 13.5%, and the economic impact in the region was estimated over USD $800.000 per year. The financial impact was greater in small- and medium-sized farms than large farms, and it was associated with the severity of SCC per quarter. Principal component analysis showed interactions, irrespective of the individual effect, and suggested three main groups of control interventions: application of basic milking hygiene practices, increase in the level of hygiene practices and veterinary advice, and SCM diagnosis and dry-cow treatment. Lack of information on management and production at farms promotes intuitive decision-making. Further research for the deeper understanding of intervention costs and effectiveness is suggested.

In vitro activity of meropenem/vaborbactam and characterisation of carbapenem resistance mechanisms among carbapenem-resistant Enterobacteriaceae from the 2015 meropenem/vaborbactam surveillance programme.

The activity of meropenem/vaborbactam was evaluated against 11 559 Enterobacteriaceae isolates, including 330 carbapenem-resistant phenotypes (CRE) and carbapenemase genotypes collected worldwide during 2015. Antimicrobial susceptibility testing for meropenem/vaborbactam (inhibitor at 8 mg/L) and comparators was performed by the reference broth microdilution method. CRE isolates were screened for the presence of genes encoding carbapenemases, and 292 (88.5%) of the CRE isolates carried these resistance genes. A total of 209 isolates (63.3% of the CRE; 1.8% of the overall Enterobacteriaceae population) carried blaKPC, including genes encoding KPC-2 (90 isolates), KPC-3 (117 isolates) and KPC-17 (2 isolates). Overall, meropenem/vaborbactam (vaborbactam at 8 mg/L) inhibited 99.3% of all Enterobacteriaceae isolates at the US-FDA susceptibility breakpoint of ≤4/8 mg/L. Meropenem alone inhibited 96.9% of the isolates at the current CLSI susceptibility breakpoint of ≤2 mg/L. Susceptibility rates for comparator antimicrobial agents tested against Enterobacteriaceae isolates ranged from 82.1-98.2% applying the CLSI breakpoints. Against CRE isolates, meropenem/vaborbactam displayed MIC50/90 values at 0.5/32 mg/L, whereas meropenem MIC50/90 values were 16/>32 mg/L. Meropenem/vaborbactam was very active against KPC-producers, and 99.5% of these isolates were inhibited by ≤4/8 mg/L. The single resistant isolate was shown to harbour an outer membrane porin alteration. Meropenem/vaborbactam had limited activity against MBL-producing isolates (including 49 NDM-, 1 IMP-64- and 2 VIM-producers) and/or oxacillinases (47 OXA-48/-232) that were detected mainly in European countries. Meropenem/vaborbactam was active against contemporary CRE and wild-type Enterobacteriaceae collected worldwide and this combination demonstrated enhanced activity compared with meropenem and most comparator agents against CRE and KPC-producers.

Conformidade de leite cru refrigerado de unidades de produção familiares do sul do Espírito Santo / Conformity of refrigerated raw milk from family production units of southern Espírito Santo

Dairy farming is an important activity in Brazil and Espírito Santo state, with small properties accounting for 80% of production. Despite of this economic importance, data about quality of the milk produced and the hygienic-sanitary conditions are still scarce. Then, the objective of this study was to evaluate the characteristics of refrigerated raw milk produced in family production units in southern Espírito Santo to verify compliance with Brazilian legal standards. Three collections were carried out in 29 community expansion tanks, for a total of 87 samples of refrigerated raw milk. Analysis of standard plate count (SPC), psychrotrophic bacteria count (PBC), somatic cell count (SCC), titratable acidity, density, fat, total dry extract (TDE), non-fat dry extract (NFDE), residues of antibiotics (β-lactam and tetracycline), cadmium and lead levels were performed. Of the 87 samples, 66% presented non-standard values for SPC, and 38% had non-standard values for SCC. Eighty five percent of the samples presented non-standard results for NFDE, 10.3% for density and 2.3% for titratable acidity. All samples complied with the legislation regarding residues of antibiotics, Cd and Pb levels. These results indicated failures in the raw milk obtainment and storage chain from family production units of southern Espírito Santo, Brazil.(AU)

A pecuária de leite é uma atividade importante no Brasil e no Espírito Santo, sendo as pequenas propriedades responsáveis por 80% da produção estadual. Mesmo com essa importância econômica para o estado, dados a respeito da qualidade do leite produzido e das condições higiênico-sanitárias de produção, ainda são escassos. Objetivou-se avaliar a qualidade do leite produzido em unidades de produção familiares, no sul do Espírito Santo, para verificar o atendimento aos padrões legais. Foram realizadas três coletas em 29 tanques comunitários totalizando 87 amostras. Realizaram-se análises de contagem padrão em placa (CPP), contagem de bactérias psicrotróficas (CBP), contagem de células somáticas (CCS), acidez titulável, densidade, gordura, extrato seco total (EST), extrato seco desengordurado (ESD), pesquisa de resíduos de antibióticos (β-lactâmicos e tetraciclina) e presença de cádmio e chumbo. Das 87 amostras, 66% apresentaram valores fora do padrão para CPP e 38% para CCS. Quanto às análises físico-químicas, 85% das amostras apresentaram resultados fora do padrão para ESD, 10,3% para densidade e 2,3% para acidez titulável. Todas as amostras atenderam a legislação quanto a presença de resíduos de antibióticos, teores de cádmio e chumbo. Dessa forma, esses resultados indicam falhas em uma ou mais etapas da cadeia de obtenção e armazenamento do leite cru.(AU)

Conformidade de leite cru refrigerado de unidades de produção familiares do sul do Espírito Santo / Conformity of refrigerated raw milk from family production units of southern Espírito Santo

Dairy farming is an important activity in Brazil and Espírito Santo state, with small properties accounting for 80% of production. Despite of this economic importance, data about quality of the milk produced and the hygienic-sanitary conditions are still scarce. Then, the objective of this study was to evaluate the characteristics of refrigerated raw milk produced in family production units in southern Espírito Santo to verify compliance with Brazilian legal standards. Three collections were carried out in 29 community expansion tanks, for a total of 87 samples of refrigerated raw milk. Analysis of standard plate count (SPC), psychrotrophic bacteria count (PBC), somatic cell count (SCC), titratable acidity, density, fat, total dry extract (TDE), non-fat dry extract (NFDE), residues of antibiotics (β-lactam and tetracycline), cadmium and lead levels were performed. Of the 87 samples, 66% presented non-standard values for SPC, and 38% had non-standard values for SCC. Eighty five percent of the samples presented non-standard results for NFDE, 10.3% for density and 2.3% for titratable acidity. All samples complied with the legislation regarding residues of antibiotics, Cd and Pb levels. These results indicated failures in the raw milk obtainment and storage chain from family production units of southern Espírito Santo, Brazil.

A pecuária de leite é uma atividade importante no Brasil e no Espírito Santo, sendo as pequenas propriedades responsáveis por 80% da produção estadual. Mesmo com essa importância econômica para o estado, dados a respeito da qualidade do leite produzido e das condições higiênico-sanitárias de produção, ainda são escassos. Objetivou-se avaliar a qualidade do leite produzido em unidades de produção familiares, no sul do Espírito Santo, para verificar o atendimento aos padrões legais. Foram realizadas três coletas em 29 tanques comunitários totalizando 87 amostras. Realizaram-se análises de contagem padrão em placa (CPP), contagem de bactérias psicrotróficas (CBP), contagem de células somáticas (CCS), acidez titulável, densidade, gordura, extrato seco total (EST), extrato seco desengordurado (ESD), pesquisa de resíduos de antibióticos (β-lactâmicos e tetraciclina) e presença de cádmio e chumbo. Das 87 amostras, 66% apresentaram valores fora do padrão para CPP e 38% para CCS. Quanto às análises físico-químicas, 85% das amostras apresentaram resultados fora do padrão para ESD, 10,3% para densidade e 2,3% para acidez titulável. Todas as amostras atenderam a legislação quanto a presença de resíduos de antibióticos, teores de cádmio e chumbo. Dessa forma, esses resultados indicam falhas em uma ou mais etapas da cadeia de obtenção e armazenamento do leite cru.

Biosecurity in the production of swine / Biosseguridade na produção de suínos

The pig farming has been highlighted globally over the last few years. In Brazil the production of swine deserves to be recognized in growth terms owed mainly the changes in the breeding systems. With the integration model, the producers started to have greater contribution and technical orientation, as well as a pre-established sale with the integrating company. As a result, swine production developed rapidly in three southern states (Paraná, Santa Catarina and Rio Grande do Sul) that have the largest swine herds in the country. This work had as an objective the current situation of piglets production units the northwestern region of Rio Grande do Sul the main items that make up the biosecurity program, identifying critical points and listing opportunities to improve the process.The adopted methodology consisted of the application of semi structured questionnaire and visiting the properties of the integrated producers. As a result, it was observed that (27.3%) of the farms produce Weaned Pigs, and (72.7%) are units producing pigs without day care. Regarding the enclosure, where 72.6% of the farms answered the question, another item was the access to water for animal watering, where 54.5% have access via an artesian well. Most of the units studied reached the objectives and standards of biosafety, thus guaranteeing the health and quality of the pork produced, but there were alsoopportunities for improvements in relation to some of the requirements, among which the enclosure,chlorination of water for Animal welfare, the location of treatment lagoons and aspects related to thedischarge and loading of animals.

A suinocultura vem se destacando mundialmente nos últimos anos. No Brasil a produção de suínos merece destaque em termos de crescimento devido principalmente as mudanças nos sistemas de criação. Com o modelo de integração, os produtores passaram a ter maiores aportes e orientações técnicas, bem como uma venda pré-estabelecida com a empresa integradora. Com isso, a produção de suínos desenvolveu-se rapidamente nos três Estados do Sul (Paraná, Santa Catarina e Rio Grande do Sul) que possuem os maiores rebanhos de suínos do País. Este trabalho teve como objetivo avaliar a situação atual das unidades produtoras de leitões da região Noroeste do Rio Grande do Sul acerca dos principais itens que compõem o programa de biosseguridade, identificando pontos críticos e elencando oportunidades de melhoria. A metodologia adotada consistiu na aplicação de questionário semiestruturado e visitas às propriedades dos produtores integrados. Com os resultados, observou-se que 27,3% das granjas são Unidades Produtoras de Leitões Desmamados, e 72,7% são Unidades Produtoras de Leitões Descrechados. Com relação ao cercamento, 72,6% das granjas atenderam ao quesito, outro item apontado foi o acesso a água para dessedentação animal, onde 54,5% possuem acesso por via de poço artesiano. A maioria das unidades estudadas atingiu os objetivos e normas de biosseguridade, garantindo assim a sanidade e qualidade da carne suína produzida, mas também se observaram oportunidades de melhorias em relação a alguns quesitos, destacando-seentre estes o cercamento, a cloração de água para dessedentação animal, a localização das lagoas detratamento e os aspectos relacionados à descarga e carga de animais.

Biosecurity in the production of swine / Biosseguridade na produção de suínos

The pig farming has been highlighted globally over the last few years. In Brazil the production of swine deserves to be recognized in growth terms owed mainly the changes in the breeding systems. With the integration model, the producers started to have greater contribution and technical orientation, as well as a pre-established sale with the integrating company. As a result, swine production developed rapidly in three southern states (Paraná, Santa Catarina and Rio Grande do Sul) that have the largest swine herds in the country. This work had as an objective the current situation of piglets production units the northwestern region of Rio Grande do Sul the main items that make up the biosecurity program, identifying critical points and listing opportunities to improve the process.The adopted methodology consisted of the application of semi structured questionnaire and visiting the properties of the integrated producers. As a result, it was observed that (27.3%) of the farms produce Weaned Pigs, and (72.7%) are units producing pigs without day care. Regarding the enclosure, where 72.6% of the farms answered the question, another item was the access to water for animal watering, where 54.5% have access via an artesian well. Most of the units studied reached the objectives and standards of biosafety, thus guaranteeing the health and quality of the pork produced, but there were alsoopportunities for improvements in relation to some of the requirements, among which the enclosure,chlorination of water for Animal welfare, the location of treatment lagoons and aspects related to thedischarge and loading of animals.(AU)

A suinocultura vem se destacando mundialmente nos últimos anos. No Brasil a produção de suínos merece destaque em termos de crescimento devido principalmente as mudanças nos sistemas de criação. Com o modelo de integração, os produtores passaram a ter maiores aportes e orientações técnicas, bem como uma venda pré-estabelecida com a empresa integradora. Com isso, a produção de suínos desenvolveu-se rapidamente nos três Estados do Sul (Paraná, Santa Catarina e Rio Grande do Sul) que possuem os maiores rebanhos de suínos do País. Este trabalho teve como objetivo avaliar a situação atual das unidades produtoras de leitões da região Noroeste do Rio Grande do Sul acerca dos principais itens que compõem o programa de biosseguridade, identificando pontos críticos e elencando oportunidades de melhoria. A metodologia adotada consistiu na aplicação de questionário semiestruturado e visitas às propriedades dos produtores integrados. Com os resultados, observou-se que 27,3% das granjas são Unidades Produtoras de Leitões Desmamados, e 72,7% são Unidades Produtoras de Leitões Descrechados. Com relação ao cercamento, 72,6% das granjas atenderam ao quesito, outro item apontado foi o acesso a água para dessedentação animal, onde 54,5% possuem acesso por via de poço artesiano. A maioria das unidades estudadas atingiu os objetivos e normas de biosseguridade, garantindo assim a sanidade e qualidade da carne suína produzida, mas também se observaram oportunidades de melhorias em relação a alguns quesitos, destacando-seentre estes o cercamento, a cloração de água para dessedentação animal, a localização das lagoas detratamento e os aspectos relacionados à descarga e carga de animais.(AU)