Maternal preconception glucose intolerance and fatty acid intake from conception to weaning: impact on offspring energy homeostasis in both male and female.

Environmental factors in the early life stages can lead the descendant to adaptations in gene expression, permanently impacting several structures and organs. The amount and quality of fatty acids in the maternal diet in pregnancy and lactation were found to impact offspring metabolism. So, maternal diet and insulin resistance can affect the male and female descendants through distinct pathways and at different time points. We hypothesized that maternal high-fat diet (HFD) intake before conception and an adequate amount of different fatty acids intake during pregnancy and lactation could influence the energy homeostasis system of 21-day-old offspring. Female rats received control diet (C) or HFD (HF) for 8 weeks before pregnancy. During pregnancy and lactation C group remained with same diet (C-C), HF group were distributed into 4 groups and received C diet (HF-C), normolipidic diet based on saturated fatty acids (HF-S) or based on polyunsaturated fatty acids n-3 (HF-P) or remained in same diet (HF-HF). Maternal HFD in preconception, pregnancy, and lactation (HF-HF) led to lower glucagon-like peptide-1 levels in male (HF-HF21) compared to other groups (C-C21, HF-C21, and HF-P21) and compared to HF-HF21 females. Neuropeptide YY levels were higher in the HF-HF21, HF-C21, and HF-S21 male offspring compared to HF-P21. HF-P21 was similar to C-C21. Positive correlations were found among the energy homeostasis markers genes expressed in the offspring hypothalamus. Maternal diet changes to adequate quantities of fatty acids during pregnancy and lactation showed less impaired results but was not entirely avoided. A maternal diet based on PUFA n-3 during pregnancy and lactation seems to reverse the damage of an HFD in preconception. These results of homeostasis energy system disturbance in the offspring at weaning give us clues about changes that precede the onset of the disease in adult life - adding notes to the knowledge for future investigations of prevention and treatment of chronic diseases.

Multivariate analysis of herd structure and genetic resource indicators in seedstock beef cattle herds.

Genetic, environmental, technological and financial resources are used differently in cattle herds that participate in the same breeding programme. The percentages of calves sired by sires within their own herd or from external herds vary across herds, as do the intensities of use of reproductive biotechnologies. These divergences may be related to differences in the indicators of genetic performance for economic traits. The aim of this study was to determine the factors related to herd structure and genetic resource utilization that exert the greatest influence on the genetic merit of seedstock herds within a Nellore breeding programme. The database comprised 21 factors, along with genomic-enhanced expected progeny differences (GE-EPDs) for growth, reproductive and carcass traits, as well as a selection index of animals from 128 herds. By combining principal component analysis and cluster analysis, we were able to group the herds. We identified statistically significant differences (p < 0.05) in the mean values of the factors, GE-EPDs and genetic trends among the groups of herds. Differences in the percentage of sires from external herds and in sire age between the groups of herds were the factors most associated with differences in mean GE-EPDs and genetic trends. Using young sires from other herds or lineages is an effective strategy in animal breeding. By enhancing genetic variability, this approach does not only improve the genetic quality of herds but also accelerates genetic progress in desired traits over time. Therefore, to ensure the success of this strategy, it is crucial that seedstock herds undergo a thorough selection process aimed at maximizing the genetic potential of future generations of beef cattle.

Effects of inclusion of the blend of essential oils, organic acids, curcumin, tannins, vitamin E, and zinc in the maternal diet, and of incubation temperature on early and late development of quail.

The maternal diet and egg incubation temperature are some of the factors that can influence the embryonic development and performance of the newly chicks at 15 d of age. This study evaluated the effects of adding a blend of organic acids, essential oils, curcumin, tannins, vitamin E, and zinc microencapsulated in to the diet of female quails (Coturnix coturnix japonica) on their productive, reproductive performance and redox parameters of their eggs and the interaction of maternal diet × incubation temperature on embryo (E16 and E18) and chicks development. At 98 d of age, 64 female quails with a mean body weight of 150 g ± 0.5 were distributed into two treatments: a Basal diet or a diet supplemented with blend (Sannimix). The eggs from each female were incubated at 37.5°C (Control) and 38.5°C (High Temperature) throughout the incubation period. After hatching, chicks were distributed in a 2 (maternal diet) × 2 (incubation temperature) factorial design. Female quails supplemented with Sannimix showed better productive and reproductive performance and produced higher-quality embryos. Their offspring had greater weight at hatch and at 15 d of age. The eggs and offspring of supplemented with Sannimix female quails showed better oxidative stability. At E16 and E18, High Temperature increased yolk sac utilization and gene expression of the growth hormone receptor (GHR). At E16, embryos from supplemented with Sannimix female quail had higher expression of insulin-like growth factor type I (IGFI) and heat shock protein 70 kDa genes. At 15 d of age, highest expression of the GHR and IGFI genes was observed in chicks from female quails fed the Sannimix diet, regardless of incubation temperature. Regarding the maternal diet × incubation temperature an improved result was observed for chicks from female quails fed with Sannimix even when eggs are exposed to High Temperature during the incubation. The supplementation of quail diets with blend Sannimix improves productive and reproductive performance, egg quality and their embryos, as well as their offspring quality.

Factors important for bull purchasing decisions and management in extensive rangeland production systems of New Mexico: a producer survey.

There were 463,000 head of beef cows in New Mexico as of January 1, 2021 (NASS, 2020), down roughly 4% from 2020 (NASS, 2019). Frequent drought often results in herd reduction and loss of valuable genetic progress. Bull selection is critical due to their influence on herd development. A survey was conducted to identify traits important to bull selection in New Mexico. Surveys were collected digitally (n = 83) and via the mail (n = 74). Responses were largely by cow/calf producers averaging 57 ± 1 years old with 24 ± 1 years' experience. Survey respondents represented 4,384,296 acres of private owned, private leased, and leased public rangeland and irrigated pasture meadow in New Mexico and surrounding states. Average cow/calf operation size was 294 ± 39 head and average bull herd size was 21 ± 3 head. Average price paid for a bull in the past 2 years was $3,981 ± 213. Physical characteristics, individual bull performance information, and genetic information are all important traits to New Mexico bull buyers; however, most producers (56%) indicated that structural soundness was the most important factor influencing their selection decisions. Amongst expected progeny differences (EPDs), New Mexico producers consider the calving ease direct (CED) and birth weight (BW) EPDS to be most important (40% and 35%, respectively). Producers also indicated that multitrait selection indexes used by the American Angus Association were important to their selection decisions, with the beef value ($B) and weaned calf value ($W) indexes being cited most often (35% and 31%, respectively). Elements important to bull purchase include the bull's sale preview (87%), body condition score (86%), feed efficiency/average daily gain information (85%), and actual scrotal circumference (82%). Following purchase of a new bull, most (60%) keep the bull separate from the cow herd until the following breeding season, while the remaining 40% of producers turn newly purchased bulls out within 30 days of purchase. Sixty eight percent of producers evaluate semen quality annually or prior to the start of the breeding season. Interestingly, 39% of producers indicated they used reproductive technologies like artificial insemination and synchronization of estrus while most (80%) test for trichomoniasis. The primary factor influencing culling decisions is age, followed by soundness and fertility.

Genetic parameters and genetic trends for production traits in dairy Gir cattle / Parâmetros genéticos e tendências genéticas de características de produção de bovinos Gir Leiteiro

The objective of this research was to estimate genetic parameters and genetic trends (GT) for 305-day milk yield (MY305) and 305-day fat yield (FY305) of purebred Dairy Gir animals of the National Dairy Gir Breeding Program. The restricted maximum likelihood method was used in an animal model. GT were obtained via linear regression and divided into two periods (1935-1992 and 1993-2013 for PL305; 1935-1992 and 1993-2010 for MY305). The estimated heritabilities were 0.23 (MY305) and 0.10 (FY305). The GT (kg/year) values for MY305 in the 2nd period for measured females (25.49), females (26.11), and males (35.13) were higher than those found in the 1st period (2.52; 2.06, and 1.00, respectively). The heritability estimated for MY305 confirmed the possibility of genetic improvement by selection and indicated a lower additive genetic effect on FY305 of purebred animals. The genetic progress for MY305 in all purebred population is denoted by the more expressive gains found from 1990's, when the first bull catalogs were published.

Objetivou-se estimar os parâmetros genéticos e tendências genéticas (GT) para produção de leite (MY305) e produção de gordura (FY305), ambas em 305 dias, de animais puros Gir Leiteiro, integrantes do Programa Nacional de Melhoramento do Gir Leiteiro. Foi utilizada a metodologia da máxima verossimilhança restrita em modelo animal. As GT foram obtidas via regressão linear e divididas em dois períodos (1935-1992 e 1993-2013 para PL305; 1935-1992 e 1993-2010 para MY305). As herdabilidades foram de 0,23 (MY305) e 0,10 (FY305). Para PL305, as GT (kg/ano) do 2º período para fêmeas mensuradas (25,49), fêmeas (26,11) e, machos (35,13) foram claramente superiores às do 1º período (2,52; 2,06 e 1,00; respectivamente). A estimativa de herdabilidade para MY305 reafirma ser possível melhoramento genético por meio de seleção, enquanto para FY305 sugere uma menor influência genética aditiva em animais puros. O progresso genético para MY305 em toda a população pura está evidenciado pelos ganhos mais expressivos, observados a partir da década de 90, quando foram divulgados os primeiros sumários de touros.

Maternal supplementation of different trace mineral sources on broiler breeder production and progeny growth and gut health.

Trace mineral minerals Zn, Cu, and Mn play important roles in breeder production and progeny performance. The objective of this study was to determine maternal supplementation of trace mineral minerals on breeder production and progeny growth and development. A total of 540 broiler breeders, Cobb 500 (Slow feathering; 0-66 weeks old) were assigned to one of three treatment groups with the same basal diet and three different supplemental trace minerals: ITM-inorganic trace minerals in sulfates: 100, 16, and 100 ppm of Zn, Cu, and Mn respectively; MMHAC -mineral methionine hydroxy analog chelate: 50, 8, and 50 ppm of bis-chelated MINTREX®Zn, Cu and Mn (Novus International, Inc.), and TMAAC - trace minerals amino acid complex: 50, 8, and 50 ppm of Zn, Cu, and Mn. At 28 weeks of age, eggs from breeder treatments were hatched for progeny trial, 10 pens with 6 males and 6 female birds per pen were fed a common diet with ITM for 45 days. Breeder production, egg quality, progeny growth performance, mRNA expression of gut health associated genes in breeder and progeny chicks were measured. Data were analyzed by one-way ANOVA; means were separated by Fisher's protected LSD test. A p-Value ≤ 0.05 was considered statistically different and 0.1 was considered numerical trend. Breeders on ITM treatment had higher (p < 0.05) body weight (BW), weight gain and lower (p < 0.05) feed conversion ratio (FCR) from 0 to 10 weeks, when compared to birds fed MMHAC. MMHAC significantly improved egg mass by 3 g (p < 0.05) and FCR by 34 points (0.05 < p < 0.1) throughout the reproductive period (26-66 weeks) in comparison to ITM. MMHAC improved (p < 0.01) egg yolk color versus (vs.) ITM and TMAAC in all periods, except 28 weeks, increased (p < 0.01) eggshell thickness and resistance vs. TMAAC at 58 weeks, and reduced (p < 0.05) jejunal NF-κB gene expression vs. TMAAC at 24 weeks. There was a significant reduction in tibial dry matter weight, Seedor index and resistance for the breeders that received MMHAC and/or TMAAC when compared to ITM at 18 weeks. Lower seedor index but numerically wider tibial circumference was seen in hens fed MMHAC at 24 weeks, and wider tibial circumference but lower tibial resistance in hens fed TMAAC at 66 weeks. Maternal supplementation of MMHAC in breeder hens increased (p < 0.0001) BW vs. ITM and TMAAC at hatching, reduced (p < 0.05) feed intake vs. ITM at d14 and d28, and improved (p < 0.01) FCR and performance index vs. TMAAC at d28, reduced (p < 0.01) NF-κB gene expression and increased (p < 0.05) A20 gene expression vs. TMAAC on d0 and vs. ITM on d14, reduced (p < 0.05) TLR2 gene expression vs. ITM on d0 and vs. TMAAC on d14, increased (p < 0.05) MUC2 gene expression vs. both ITM and TMAAC on d45 in progeny jejunum. Overall, these results suggest that supplementation with lower levels of MHA-chelated trace minerals improved breeder production and egg quality and reduced breeder jejunal inflammation while maintaining tibial development in comparison to those receiving higher inorganic mineral supplementation, and it also carried over the benefits to progeny with better growth performance, less jejunal inflammation and better innate immune response and gut barrier function in comparison to ITM and/or TMAAC.

Methionine Supplementation during Pregnancy of Goats Improves Kids' Birth Weight, Body Mass Index, and Postnatal Growth Pattern.

The last third of gestation is a period of high energy and protein demand for the dam to support fetal growth and the following onset of lactation. Methionine is an essential amino acid that contributes to protein formation, fetal development, and milk synthesis; thus, is likely to have positive effects on the weight and size of the newborn and, afterward, milk yield and milk composition, which may improve growth patterns of the progeny. To test these hypotheses, we used 60 pregnant multiparous Alpine goats with similar live weights and gestational ages (~Day 100 of pregnancy; Mean ± SD; 1410 ± 14 days old and 50.4 ± 6.6 kg) and were separated into two groups: control and supplemented with the delivery. Treatments were T-MET (n = 30; received 1% herbal methionine Optimethione® dry matter based on from Day 100 of the pregnancy to delivery) or T-CTL (n = 30; served as the control and did not receive methionine). The methionine powder provided individual supplementation and was adjusted every week as the live weight and dry matter intake changed. At birth, the weight, body mass index (BMI), birth type, and sex of the kids were determined. Subsequently, the progeny was weighed weekly up to weaning. Two weeks after parturition, the milk composition was recorded weekly, and the milk yield was recorded monthly. The maternal live weight at the start (Mean ± SEM; T-CTL: 50.5 ± 1.1 vs. T-MET: 50.3 ± 1.3 kg) and end (T-CTL: 54.2 ± 1.3 vs. T-MET: 52.8 ± 1.4 kg) of the experiment did not differ statistically among treatments (p > 0.05); however, daily live weight changes tended to differ between groups (T-CTL: 73 ± 10 vs. T-MET: 51 ± 7 g day−1; p = 0.06). The birth weight (T-CTL: 3.1 ± 0.1 vs. T-MET: 3.5 ± 0.1 kg; p < 0.001), daily live weight change (T-CTL: 121 ± 6 vs. T-MET: 141 ± 6 g day−1; p < 0.01), and weaning weight (T-CTL: 8.3 ± 0.2 vs. T-MET: 9.3 ± 0.3 kg; p < 0.01) differed between treatments. The BMI at birth (T-CTL: 0.28 ± 0.01 vs. T-MET: 0.3 ± 0.01 units kg m−2; p < 0.01) and at weaning (T-CTL: 0.85 ± 0.1 kg vs. T-MET: 1.00 ± 0.06 units kg m−2; p < 0.05) differed between treatments. Milk components (protein, fat, lactose, and solids non-fat) and milk yield were similar between treatments (p > 0.05). It is concluded that the inclusion of methionine in the maternal goat diet during the last third of gestation increases the birth and growth variables of the progeny but without significant influence on the milk yield and composition.

Variation of Residual Sexuality Rates along Reproductive Development in Apomictic Tetraploids of Paspalum.

Most apomictic plants are facultative, maintaining the ability to reproduce sexually at different frequencies depending on the taxa, ploidy, and reproductive stage. In this context, Paspalum species are good model systems for studies evaluating the varying levels of apomixis expression. We aimed to identify, in apomictic tetraploid Paspalum species, the degree of apomixis and residual sexuality in three stages of reproductive development, and if their expression varies along them in order to predict their realized impact on the genetic diversity of future generations. Three main stages in the reproductive development (i.e., ovule, seed, and progeny) were studied in tetraploids from populations of P. cromyorhizon and P. maculosum. Mature ovules were studied using cytoembryological analysis, seeds by flow cytometry, and progeny tests with molecular markers. The expression of sexuality and apomixis was compared in each stage. We observed a decline in expression of sexual reproduction through the consecutive stages, jointly with an increase of apomixis expression. Both species showed at least one tetraploid plant capable of producing progeny by sexual means. These small rates of sexually originated progeny prove the ability of apomictic plants to produce low levels of genetic variation through rare events of sexuality. This study also demonstrates the importance of analyzing different reproductive stages in order to get a whole picture of the reproductive outcomes in plant evolution.

Insecticidal properties of etofenprox for the control of Ephestia kuehniella, Rhyzopertha dominica, Sitophilus oryzae, and Tribolium confusum on stored barley, maize, oats, rice, and wheat.

Etofenprox is a novel pyrethroid insecticide that targets the nervous system of insects by affecting the function of the sodium channel. The current study examines the insecticidal activity of etofenprox against Ephestia kuehniella (Lepidoptera: Pyralidae) larvae, Rhyzopertha dominica (Coleoptera: Bostrychidae) adults, Sitophilus oryzae (Coleoptera: Curculionidae) adults, and Tribolium confusum (Coleoptera: Tenebrionidae) adults and larvae on different grain commodities. For this purpose, etofenprox was applied on barley, maize, oats, rice, and wheat at 0.1, 1, 5, and 10 ppm. Mortality levels were recorded after 7, 14, and 21 days of exposure. For E. kuehniella larvae, 10 ppm applied on whole rice killed 96.1% of the exposed individuals after 21 days of exposure. The application of etofenprox on oats at 5 ppm caused the death of 98.3% of the exposed R. dominica adults. Complete mortality was observed for R. dominica adults 21 days post-exposure to oats and whole rice treated with 10 ppm etofenprox. The highest concentration applied on barley caused 95.0% mortality to S. oryzae adults, while the same concentration on maize killed 76.8% of T. confusum adults after 21 days of exposure. Larvae of T. confusum exhibited high mortality levels reaching 99.4% after 21 days of exposure to barley treated with 10 ppm etofenprox. Progeny production of parental R. dominica adults was almost suppressed on all commodities treated with 5 ppm etofenprox. The offspring emergence of S. oryzae ranged between 4.4 and 24.6 adults per vial at 10 ppm. No T. confusum progeny was produced at 10 ppm etofenprox. Our results document that etofenprox is highly effective as grain protectant against several insect species, their developmental stages and their progeny production, but its performance depends on the type of the commodity that it is applied on.

Comparative analysis of radon gas concentration in caves developed in carbonatic, quartzitic and ferruginous lithologies.

Radon gas concentrations in ferruginous, quartzitic/conglomeratic and carbonatic caves were measured and the results obtained in each lithology were compared. Three representatives of each formation (nine caves in total) were studied. The caves are located in three environmental preserved areas in the Ferriferous-Aquifer Quadrangle and in its regional surrounding, in the center-south portion of Minas Gerais State, Brazil. It was found that the average concentration in quartzitic/conglomeratic caves (4,759 Bq/m3) was much higher than those obtained in carbonatic (844 Bq/m3) and ferruginous (945 Bq/m3) caves. This result most likely reflects differences in the uranium content of the rock types. Also, for a better understanding of the radon distribution inside the caves, concentration maps with their interpolated values were generated using geospatial analysis tools.

Low genetic variation of foliar traits among Prosopis chilensis (Leguminosae) provenances.

Prosopis chilensis (Molina) Stuntz (Leguminosae) is a valuable native species in Argentina that has been proposed to be used in reforestation, afforestation and restoration programmes. Natural provenances show important differentiation in height, shape, spine size, fruits and foliar traits throughout their distribution in the semiarid Monte ecoregion. The goal of this work was to characterize the genetic basis of the leaf variation in P. chilensis aiming to contribute to the improvement management program. We analyzed morphological variation and estimate narrow sense heritability for ten quantitative traits from a provenance-progeny trial founded from open pollinated families. We assessed the variance components by a generalized linear mixed model. Differences among provenances were quantified through univariate QST statistics and multivariate discriminant analysis of principal components. Finally, univariate and multivariate neutrality test were conducted to unveil the evolutionary forces that shape the variation. Univariate and multivariate analysis showed low genetic variation in foliar traits among provenances grown in the common garden. Consistently, the QST estimates for each trait were low. Both, the univariate (QST-FST comparison) and the multivariate neutrality test suggest that the leaf variation among provenances may be shaped by genetic drift rather than selective forces. Heritability estimates were significant only for leaflet apex and leaflet apex/leaflet area. Since genetic variation for most foliar traits among provenances estimated under controlled environmental conditions were very low or absent, the variation described in the wild would be explained merely by plastic response to varying environments. These results are discussed in terms of adaptive strategies and the use of different provenances as seed sources within the framework of the improvement program. It is expected that P. chilensis seeds or seedlings from trees selected under economical criteria will be able to develop in different areas thanks to the phenotypic plasticity of leaf traits.

Genetic diversity of Peltophorum dubium (Spreng.) Taub. progenies from the states of Minas Gerais and Mato Grosso do Sul, Brazil / Diversidade genética de progênies de Peltophorum dubium (Spreng.) Taub. procedentes de Minas Gerais e Mato Grosso do Sul, Brasil

There is little information regarding the genetic diversity of native species aimed at identifying the best viable progenies for in situ and ex situ conservation. Furthermore, there is a lack of future forest improvement programs. We aimed to know the genetic diversity of 64 Peltophorum dubium (Spreng.) Taub. (Fabaceae) progenies. We determined this species' dendrometric characteristics, and when using multivariate techniques and cluster analysis, we verified the differences between the progenies and groups with less heterogeneity. The progeny and provenance test was installed in Dourados (Mato Grosso do Sul - MS), with seeds collected in three MS regions (Vale do Ivinhema, Serra de Maracaju, and Serra da Bodoquena) and in the micro-region of Lavras (Minas Gerais - MG). The experiment was conducted in an alpha lattice 8 x 8 with four repetitions. We found genetic variability among and within P. dubium populations for all height, diameter, circumference at breast height, volume, and basal area characters. We suggest that P. dubium populations have high genetic variability, which indicates possible genetic improvement through best progeny selection. The UPGMA and Tocher methods grouped the progenies into three and nine groups, respectively, in which the most divergent individuals come from MG and the Bonito region in MS. Based on morphological characters, P. dubium progenies identified as 45, 47, 49, 50, 55, and 59 from MG are the most promising, while progenies 6 and 9 were the least promising.

Informações quanto à diversidade genética de espécies nativas visando identificar melhores progênies viáveis para conservação in situ e ex situ, além de futuros programas de melhoramento florestal são incipientes. Objetivamos conhecer a diversidade genética de 64 progênies de Peltophorum dubium (Spreng.) Taub. (Fabaceae). Determinamos os caracteres dendrométricos dessa espécie, e ao utilizarmos técnicas multivariadas e análises de agrupamento verificamos as diferenças entre as progênies e grupos de menor heterogeneidade. O teste de progênies e procedências foi instalado em Dourados (MS), cujas sementes foram coletadas em três regiões de MS (Vale do Ivinhema, Serra de Maracaju e Serra da Bodoquena) e na microrregião de Lavras-MG. O experimento foi conduzido em alfa-látice 8 x 8, com quatro repetições. Constatamos variabilidade genética entre e dentro das populações de P. dubium para todos os caracteres altura, diâmetro, circunferência à altura do peito, volume e área basal. Sugerimos que as populações de P. dubium tem alta variabilidade genética, o que indicou possibilidade de melhoramento genético pela seleção das melhores progênies. Os métodos UPGMA e Tocher agruparam as progênies em três e nove grupos, respectivamente, em que os indivíduos mais divergentes são procedentes de MG e da região de Bonito em MS. As progênies de P. dubium de identificação 45, 47, 49, 50, 55 e 59, todas de MG, são as mais promissoras, enquanto que as progênies 6 e 9, foram as menos promissoras, baseando-se nos caracteres morfológicos.

Genetic parameters and genetic trends of conformation and management traits in Dairy Gir cattle / [Parâmetros genéticos e tendências genéticas de características de conformação e manejo de bovinos Gir Leiteiro]

The objective of this study was to estimate genetic parameters and genetic trends of different conformation and management traits regularly measured within the context of the National Dairy Gir Breeding Program (PNMGL). The estimation of genetic and residual variances for each trait was performed using average information restricted maximum likelihood (AI-REML) procedure in AIREMLF90 program software. The population was divided into three subpopulations constituted by measured females (with phenotype records), all females, and males. Linear regressions were applied for each trait, considering two periods of birth (1st period: 1938-1996; 2nd period: 1997-2012). The estimated heritability of conformation and management traits varied from 0.01 to 0.53, denoting a perspective of genetic improvement through selection and corrective matings for purebred Dairy Gir populations. The average genetic changes in conformation and management traits were, in general, variable and inexpressive, showing that the selection of Dairy Gir may have had been directed essentially to increase milk yield. The analysis of the two periods of birth indicated that some linear traits present progress (although inexpressive) in the 2nd period (more recent period).(AU)

O objetivo deste estudo foi estimar os parâmetros genéticos e as tendências genéticas para diferentes características de conformação e manejo de animais puros da raça Gir Leiteiro, pertencentes ao Programa Nacional de Melhoramento do Gir Leiteiro (PNMGL). A estimativa das variâncias genéticas e residuais para cada característica foi realizada usando-se o procedimento de máxima verossimilhança restrita (AI-REML), por meio do programa AIREMLF90. A população foi dividida em três subpopulações, constituídas por fêmeas mensuradas (com registros de fenótipo), todas fêmeas e machos. As regressões lineares para cada característica foram ainda divididas em dois períodos de anos de nascimento (1º período: 1938 a 1996; 2º período: 1997 a 2012). As herdabilidades estimadas variaram de 0,01 a 0,53 para as características de conformação e manejo, possibilitando a perspectiva de melhoramento mediante seleção e acasalamentos corretivos na população pura da raça Gir Leiteiro. As mudanças genéticas nas características conformação e manejo foram, em geral, variáveis e inexpressivas, sugerindo que a seleção no Gir Leiteiro possa ter sido direcionada essencialmente para maior produção de leite. Ao serem observados os dois períodos distintos de anos de nascimento, infere-se que algumas características lineares apresentaram progresso (embora inexpressivo) no 2º período analisado.(AU)

Clone worth? Genetic diversity in obligate apomictic Miconia albicans (Melastomataceae).

Apomixis is the asexual production of seeds by plants and, in theory, would render low genetic diversity and even clonal lineages. However, recent studies have shown otherwise, although is not always clear where the genetic diversity of obligate apomicts comes from. We evaluated the genetic diversity among sister seedlings of M. albicans, an obligate apomictic species in Cerrado, Neotropical Savannas in Central Brazil. A total of 50 seedlings from five individuals were analysed using ISSR primers. We obtained 107 fragments, all with good resolution, consistently observed and replicable. The percentage of polymorphic loci ranged from 28.04% to 33.64% and Shannon's information index (I) averaged 0.173. The expected heterozygosity (He) averaged 0.117, similar to the observed for populations of M. albicans and other selfed species. Only two seedlings showed the same genotype (possible clones), but most differed at least for five loci. Most of variance was among progenies (62%), but we found that 38% was within progenies. Genetic distances separated the progenies in two groups, and analogous analyses between individuals reconstructed the original progenies clustering. The results confirmed a relatively high genetic diversity among sister seedling of this obligatory apomictic plant and clones were rare. This diversity can be generated during development, probably by restitutional meiosis or other recombination processes. These differences may accumulate into lineages and populations well adapted to heterogenous Cerrado environment.

Evaluation of transfer of maternal immunity to the offspring of broiler breeders vaccinated with a candidate recombinant vaccine against Salmonella Enteritidis.

Salmonella Enteritidis (SE) is a major cause of foodborne diseases in humans being frequently related to the consumption of poultry products. Therefore, guaranteeing early immunity to chicks is an important tool to prevent the colonization and infection by this pathogen. The present study evaluated the effectiveness of a candidate recombinant vaccine against SE. Thirty female and five male broiler breeders that were ten weeks-old were divided into 3 groups: unvaccinated (UV), vaccinated with recombinant vaccine candidate (VAC) and vaccinated with commercial bacterin (BAC). Samples of serum and embryonated egg were collected at seven and twelve weeks after the booster dose to quantify the transfer rate of IgY to egg yolks and offspring. Subsequently, forty day-old offspring were divided into two groups (UV and VAC) and challenged on the following day with 107 CFU/chick of SE. Samples of serum, intestine, liver, and cecal content were harvested. Throughout the experiment period, significantly higher levels of IgY were observed in the egg yolk and also in the serum of broiler breeders and offspring of the VAC group in comparison to the UV group. In addition, increased transfer rates of IgY were observed in the VAC group when compared to the BAC group. Furthermore, higher villus-crypt ratios were found out in duodenum, jejunum and ileum at four days post-infection in the offspring from the VAC group. A high challenge dose of SE (107 CFU per chick) was used and despite the stronger humoral immune response provoked by the candidate vaccine, there were no statistical differences in the recovery of viable SE cells from the offspring cecal contents. Therefore, the effect of vaccination to improve intestinal quality may affect the development of the chickens and consequently increase the resistance to lower SE challenge doses.

Fire frequency effects on cleistogamy expression and progeny performance in Cologania broussonetii.

Increased fire frequency usually erodes microenvironmental conditions, causing a drastic limitation of edaphic resources. Thus, the production of permanently closed-small flowers (cleistogamous, CL) should increase in sites with high fire frequency as this implies a less expensive reproductive assurance strategy. However, because open, insect-pollinated flowers (chasmogamous, CH) have the potential capacity to outcross via pollinators, CH progeny produced at any site should outperform selfed CL progeny. We evaluate the effect of fire frequency on the relative production of CL/CH flowers and fruits, and their seed set, along with several progeny performance parameters in Cologania broussonetii (Fabaceae), a resprouting herb with dimorphic cleistogamy native to the Chaco Serrano. Fire frequency increased cleistogamy expression, reaching extreme levels in high fire frequency sites. Seed set was similarly high for both CH and CL flowers in the unburned condition, while in burned sites the few developed CH flowers set more seeds than CL flowers. However, progeny performance was similar between CH and CL progeny at each and across all fire frequency conditions. Cleistogamy expression in C. broussonetii is maximized in abiotically degraded frequently burned habitats, although the selfed CL progeny is as successful as potentially outcrossed CH progeny. Fire frequency may decreased floral size and abundance, selecting for autogamous reproduction, which restricts not only the genetic potential of plant populations but also the resources offered to pollinators. At the community level, increased cleistogamy expression may potentially have negative implications for non-cleistogamous, more outcrossing species surviving in frequently burned environments.

Milk yield and composition and body weight of offsprings of mixed-breed goats on semi-arid rangelands with different rainfall.

The objective was to characterize milk yield and composition from mixed-breed goats kidding in winter and grazing rangelands with 221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N). Weekly milk yield and composition were recorded and progeny growth performance assessed. Body weight loss during lactation was the highest (6.4 kg) for goats on rangeland with 441 mm of rainfall than goats on 221 mm (1.6 kg), or 334 mm (1.8 kg; P < 0.01). The highest daily milk yield was for goats on 221 mm (498 mL; P < 0.01). There was not an evident peak of lactation and milk yield increased uninterruptedly at the end of lactation. Milk fat was negatively related to milk yield and goats on rangeland with 334 mm had the greatest milk fat content (P < 0.001). Milk protein and lactose content were higher in goats on rangeland with 221 mm (P < 0.01). Goat kids on rangelands with 221 and 441 mm grew faster and were heavier (P < 0.001) at weaning than kids reared in other rangelands. Weaning weight was positively related to milk yield (P < 0.001); but not milk components (P > 0.05). In conclusion, moderate milk yield of mixed-breed goats on rangeland is feasible with parturitions in winter, and milk yield is not affected by reduced annual precipitation in rangelands of northern Mexico. Offspring growth performance depends on maternal milk yield, but not milk composition. Nevertheless, slow growth to weaning of offspring had adverse effects on time to reach adequate market weight.

Effect of early shearing during gestation on the productive and reproductive behavior of female sheep offspring in their first 18 months of age.

The research has shown the interesting contributions of shearing in mid-gestation on the performance of lambs from birth to weaning. Other studies have reported that shearing at early pregnancy influences the development of the placenta and lamb live weight at birth. However, there was a lack of information on the effect of early-prepartum shearing on the behavior of the offspring from weaning onward. This study evaluated the effect of shearing ewes at 50 days of gestation on the growth, reproductive behavior and response to a gastrointestinal parasite challenge in the female offspring from weaning to 18 months old. Fifty-seven Polwarth female lambs were used, 22 being singles and 35 twins born to ewes either shorn at 50 days of pregnancy (PS, n = 23) or shorn at 62 days postpartum (U, control, n = 34) resulting in four subgroups: single lambs born to PS ewes (n = 8), born to U ewes (n = 14), twin lambs born to PS ewes (n = 15) or born to U ewes (n = 20). All progeny were managed together under improved pasture with a minimum forage allowance of 6% live weight on dry basis. Body weight, body condition score and fecal eggs count were recorded every 14 days from weaning to 18 months of age. Concentrations of progesterone were measured weekly (from 4 to 10 months of age and from 14 to 18 months of age) to establish the onset of puberty. Ovulation rate at an induced and a natural heat (545 ± 1.0 and 562 ± 1.0 day old) was recorded. Prepartum shearing did not affect the age at puberty or the ovulation rate of female offspring, but those born as singles were more precocious ( P = 0.03) and heavier ( P = 0.02) at puberty than twin born lambs. Both the average value of parasite egg count ( P = 0.0 7) and the Famacha index ( P = 0.02) for the entire study period were lower in lambs born to prepartum shorn ewes than those born to postpartum shorn ewes. In conclusion, shearing at 50 days of gestation did not affect the growth or the reproductive behavior of female offspring. However, female lambs born from ewe shorn during gestation showed a better response to the parasitic challenge, and further research is required to confirm this.

Vinasse in the diet of lactating sows and its effect on litter

The aim of this study was to verify the impact of adding vinasse as an acidifier in sows diet during the lactating period and its effect on litter. A total of 14 agroceres females were used from the first day of lactation until weaning. The vinasse was obtained in a mill near the experimental facility and was stored in sterilized barrels. Animals were distributed in a completely randomized experimental design into two treatments: control liquid diet and liquid diet with the inclusion of vinasse in each feed supply of the day. The diets were supplied four times a day, containing a mixture of ½ L of vinasse and ½ L of water. Feed leftovers were weighted daily. Feed intake was used to evaluate sows performance. The piglets were weighted every week after birth until weaning in order to obtain weight gain. Vinasse inclusion showed an increase (p < 0.05) in feed intake of lactating sows, improving diet palatability. Inclusion of acidifier in the diet did not show (p > 0.05) difference in weight gain of piglets. Vinasse consumption by sows did not influence litter performance. The inclusion of vinasse in the diet of lactating sows is beneficial because increases feed consumption.(AU)

Vinasse in the diet of lactating sows and its effect on litter

The aim of this study was to verify the impact of adding vinasse as an acidifier in sows diet during the lactating period and its effect on litter. A total of 14 agroceres females were used from the first day of lactation until weaning. The vinasse was obtained in a mill near the experimental facility and was stored in sterilized barrels. Animals were distributed in a completely randomized experimental design into two treatments: control liquid diet and liquid diet with the inclusion of vinasse in each feed supply of the day. The diets were supplied four times a day, containing a mixture of ½ L of vinasse and ½ L of water. Feed leftovers were weighted daily. Feed intake was used to evaluate sows’ performance. The piglets were weighted every week after birth until weaning in order to obtain weight gain. Vinasse inclusion showed an increase (p 0.05) difference in weight gain of piglets. Vinasse consumption by sows did not influence litter performance. The inclusion of vinasse in the diet of lactating sows is beneficial because increases feed consumption.