RESUMO
Objective To investigate the effect of repeated use on the stability of HIV quality control serum during cold storage , and put forward measures to deal with .Methods Ten HIV serum from -20 degrees celsius freezer room temperature balance out , 30 minutes to make it completely melted ,randomly arranged in the patient sample ,added sample testing according to the normal sample ,after the end of time to save 2 to 8 degrees celsius refrigerator .The next day ,the above quality control products from 2 to 8 degrees celsius refrigerator ,after 30 minutes at room temperature ,randomly arranged in the patient sample ,add sample testing ac-cording to the normal sample ,after the end of time to save 2 to 8 degrees celsius refrigerator ,repeat the above activities to seventh days .Using repeated measure ,at the same time ,took the average 10 quality control of product values on the same day as a check-pointcompared with daily quality control chart ,observe changes .Results It suggested that in different measurement on the num-ber of days the difference was statistically significant(F=48 .515 ,P<0 .01) ,combined with the quality control chart found ,which was starting from the fourth day quality value warningstatus ,began to appear and the drift of fell out of control .Conclusion HIV quality-control serum at 2 to 8 degrees celsius refrigerator ,used repeatedly during 3 days late stability is affected ,the change of quality control diagram appear drift ,even is out of control .
RESUMO
Objective To grasp bilirubin in the quality control serum and calibration items in the same batch the variation law of the designed to ensure that the test quality and the use of reasonable quality control serum and calibration items ,control testing cost .Methods laboratories use The double level quality control serum and calibration items after dilution is divided into five groups ,with automatic biochemical analyzer test for 12 weeks and recorded results of TBIL ,DBIL .use SPSS 17 .0 to calculate the mean ,standard deviation ,coefficient of variation ,Normal distribution analysis and make the results trend line chart to observe any changes .Results RANDOX normal concentration quality control serum TBIL and DBIL values variation RCV% 26 .0% ,48 .2% , more than CLIA bilirubin projects allow 1/3 of the total error value(6 .67% ) .Normal distribution test ,P value was 0 .006 ,0 .012 , less than 0 .05 ,do not obey the normal distribution ,test results line chart is on the decline .LEADMAN high concentration quality control serum and bilirubin calibration items TBIL and DBIL variation RCV% 0 .05 ,obey the normal distribution ,test results no trend line chart .Conclusion Through the experiment observation to the quality control ser‐um and calibration items bilirubin in the variation law of 12 weeks .Experimenter can be used according to the actual situation to ad‐just ,not only to ensure the quality of the bilirubin test ,and to get the most out of the quality control serum and calibration items use efficiency ,reduce the test cost .
