Uso da polpa desidratada do fruto de Mauritia flexuosa como suplemento ao diluente de congelação do sêmen caprino / Use of dehydrated pulp of Mauritia flexuosa fruit as supplement to freezing extender of goat semen

Objetivou-se avaliar a qualidade in vitro do sêmen caprino descongelado utilizando diluentesuplementado com a polpa desidratada do fruto de Mauritia flexuosa. O experimento foi dividido emduas etapas. Na primeira, foram utilizados 15 pools,fracionados em 13 tratamentos com diferentesconcentrações do extrato bruto. Os melhores resultados de viabilidade espermática obtidos na primeiraetapa foram utilizadas na segunda etapa (criopreservação). Para isto, foram formados dois gruposutilizando 15 pools, sendo um diluente constituído (TRIS + 7% glicerol + melhores concentrações doextrato bruto) e outro pelo diluente (TRIS + 2,5% gema de ovo + 7% glicerol + melhores concentraçõesdo extrato bruto). Na primeira etapa os grupos contendo baixa quantidade do extrato não diferiram dogrupo controle (P≤0,05). Todavia na segunda etapa, após descongelação, os grupos TRIS contendo 2,5%ou 0% de gema de ovo apresentaram diferença significativa, onde o grupo TB06GLGE foi superior aogrupo controle. Portanto, a polpa desidratada do fruto de Mauritia flexuosa,nas concentrações de 0,25% a1%, não atuou de forma benéfica sobre parâmetros espermáticos do sêmen caprino após acriopreservação/descongelação.(AU)

The objective was to evaluate the in vitro quality of the thawed goat semen using diluentsupplemented with the dehydrated pulp of the Mauritia flexuosa fruit. The experiment was divided intotwo stages. In the first, 15 fractionated pools were used in 13 treatments with different concentrations ofthe crude extract. The best results of sperm viability obtained in the first experimental stage were used inthe second experimental stage (cryopreservation). Afterwards, two groups were formed using 15 pools,one constituent (TRIS + 7% glycerol + best concentrations of the crude extract) and another by thediluent (TRIS + 2,5% egg yolk + 7% glycerol + best concentrations of the crude extract). In the firststage, the groups containing low amount of extract did not differ from the control group (P≤0.05).However, in the second stage, after thawing, TRIS groups containing 2.5% or 0% egg yolk presented asignificant difference, where the TB06GLGE group was superior to the control group. Therefore, thedehydrated fruit pulp of Mauritia flexuosa at concentrations of 0.25% to 1% did not benefit goat semenparameters after cryopreservation/thawing.(AU)

Uso da polpa desidratada do fruto de Mauritia flexuosa como suplemento ao diluente de congelação do sêmen caprino / Use of dehydrated pulp of Mauritia flexuosa fruit as supplement to freezing extender of goat semen

Objetivou-se avaliar a qualidade in vitro do sêmen caprino descongelado utilizando diluentesuplementado com a polpa desidratada do fruto de Mauritia flexuosa. O experimento foi dividido emduas etapas. Na primeira, foram utilizados 15 pools,fracionados em 13 tratamentos com diferentesconcentrações do extrato bruto. Os melhores resultados de viabilidade espermática obtidos na primeiraetapa foram utilizadas na segunda etapa (criopreservação). Para isto, foram formados dois gruposutilizando 15 pools, sendo um diluente constituído (TRIS + 7% glicerol + melhores concentrações doextrato bruto) e outro pelo diluente (TRIS + 2,5% gema de ovo + 7% glicerol + melhores concentraçõesdo extrato bruto). Na primeira etapa os grupos contendo baixa quantidade do extrato não diferiram dogrupo controle (P≤0,05). Todavia na segunda etapa, após descongelação, os grupos TRIS contendo 2,5%ou 0% de gema de ovo apresentaram diferença significativa, onde o grupo TB06GLGE foi superior aogrupo controle. Portanto, a polpa desidratada do fruto de Mauritia flexuosa,nas concentrações de 0,25% a1%, não atuou de forma benéfica sobre parâmetros espermáticos do sêmen caprino após acriopreservação/descongelação.

The objective was to evaluate the in vitro quality of the thawed goat semen using diluentsupplemented with the dehydrated pulp of the Mauritia flexuosa fruit. The experiment was divided intotwo stages. In the first, 15 fractionated pools were used in 13 treatments with different concentrations ofthe crude extract. The best results of sperm viability obtained in the first experimental stage were used inthe second experimental stage (cryopreservation). Afterwards, two groups were formed using 15 pools,one constituent (TRIS + 7% glycerol + best concentrations of the crude extract) and another by thediluent (TRIS + 2,5% egg yolk + 7% glycerol + best concentrations of the crude extract). In the firststage, the groups containing low amount of extract did not differ from the control group (P≤0.05).However, in the second stage, after thawing, TRIS groups containing 2.5% or 0% egg yolk presented asignificant difference, where the TB06GLGE group was superior to the control group. Therefore, thedehydrated fruit pulp of Mauritia flexuosa at concentrations of 0.25% to 1% did not benefit goat semenparameters after cryopreservation/thawing.

Use of glucose or BTS™ combined with DMSO or methylglycol under two differentfreezing protocols for the cryopreservation of sperm from the common curimatã(Prochilodus brevis)

This study evaluated the effect of glucose orBeltsville Thawing Solution (BTS™) combined withdimethyl sulfoxide (DMSO) or methylglycol (MG)under two different freezing protocols on the kineticsand morphology of cryopreserved Prochilodus brevissperm. The semen samples were diluted using one of fourdifferent treatments (glucose+DMSO, glucose+MG,BTS™+DMSO, and BTS™+MG), loaded into 0.25-mlstraws and subjected to two different freezing processes(programmed freezing machine and dry shipper). After10 days, the semen samples were thawed, and the spermmorphology and kinetics were evaluated. Thephysicochemical parameters of the semen in naturawere similar to those observed in other studies ofCharaciformes, indicating the feasibility of semencryopreservation. Glucose, when used as a diluentwith the cryoprotectant MG (glucose+MG), yieldedhigher percentages of mobile spermatozoa afterfreezing in a dry shipper (76.88 ± 4.84%) and in aprogrammed freezing machine (70.95 ± 1.76%)compared with the combination of glucose and DMSO.Moreover, the glucose+MG treatment yielded a highersperm velocity (curvilinear velocity: 79.52 ± 2.88 µm s-1; straight-line velocity: 45.46 ± 3.01 µm s-1; averagepath velocity: 67.92 ± 3.08 µm s-1) than the otherstudied treatments, and a higher amount of normalsperm (74.56 ± 0.77%) was observed in the semensamples cryopreserved using a programmed freezingmachine. The sperm abnormalities observed included abent tail morphology. Therefore, the use ofglucose+MG in combination with either a dry shipper ora programmed freezing machine is recommended forthe cryopreservation of P. brevis sperm because thesemethods yielded high numbers of motile andmorphologically normal spermatozoa.(AU)

Use of glucose or BTS™ combined with DMSO or methylglycol under two differentfreezing protocols for the cryopreservation of sperm from the common curimatã(Prochilodus brevis)

This study evaluated the effect of glucose orBeltsville Thawing Solution (BTS™) combined withdimethyl sulfoxide (DMSO) or methylglycol (MG)under two different freezing protocols on the kineticsand morphology of cryopreserved Prochilodus brevissperm. The semen samples were diluted using one of fourdifferent treatments (glucose+DMSO, glucose+MG,BTS™+DMSO, and BTS™+MG), loaded into 0.25-mlstraws and subjected to two different freezing processes(programmed freezing machine and dry shipper). After10 days, the semen samples were thawed, and the spermmorphology and kinetics were evaluated. Thephysicochemical parameters of the semen in naturawere similar to those observed in other studies ofCharaciformes, indicating the feasibility of semencryopreservation. Glucose, when used as a diluentwith the cryoprotectant MG (glucose+MG), yieldedhigher percentages of mobile spermatozoa afterfreezing in a dry shipper (76.88 ± 4.84%) and in aprogrammed freezing machine (70.95 ± 1.76%)compared with the combination of glucose and DMSO.Moreover, the glucose+MG treatment yielded a highersperm velocity (curvilinear velocity: 79.52 ± 2.88 µm s-1; straight-line velocity: 45.46 ± 3.01 µm s-1; averagepath velocity: 67.92 ± 3.08 µm s-1) than the otherstudied treatments, and a higher amount of normalsperm (74.56 ± 0.77%) was observed in the semensamples cryopreserved using a programmed freezingmachine. The sperm abnormalities observed included abent tail morphology. Therefore, the use ofglucose+MG in combination with either a dry shipper ora programmed freezing machine is recommended forthe cryopreservation of P. brevis sperm because thesemethods yielded high numbers of motile andmorphologically normal spermatozoa.