Physicochemical characterization, phenolic composition and antioxidant activity of genotypes and commercial cultivars of blueberry fruits / Caracterização físico-química, composição fenólica e atividade antioxidante de genótipos e cultivares comerciais de frutos de mirtileiro

The physicochemical characterization, antioxidant potential and phenolic composition of fruits of six genotypes (BB3, BB4, BB6, PW1, PW2 and PW5) and seven commercial cultivars (Bluebelle, Bluegem, Briteblue, Climax, Delite, Powderblue and Woodard) were carried out of blueberry, from the 2019/2020 production cycle. Color, soluble solids content, pH, titratable acidity, antioxidant activity (DPPH and ABTS), total phenolic compounds, total flavonoids, total anthocyanins and individual anthocyanins were analyzed. In general, all genotypes and cultivars are rich in phytochemicals. The genotypes PW1, PW2, PW5, BB3 and the cultivars Bluegem, Briteblue, Climax and Delite stand out. Thus, the blueberry genotypes in this study are considered as promising for the food industry and also for fresh consumption as commercial cultivars already consolidated in the fruit market, in view of the excellent phenolic composition present in these small fruits.

Fez-se a caracterização físico-química, potencial antioxidante e composição fenólica de frutos de seis genótipos (BB3, BB4, BB6, PW1, PW2 e PW5) e sete cultivares comerciais (Bluebelle, Bluegem, Briteblue, Climax, Delite, Powderblue e Woodard) de mirtileiro, oriundos do ciclo de produção 2019/2020. Analisou-se a cor, teor de sólidos solúveis, o pH, a acidez titulável, atividade antioxidante (DPPH e ABTS), compostos fenólicos totais, flavonoides totais, antocianinas totais e antocianinas individuais. De modo geral, todos os genótipos e cultivares são ricos em compostos fitoquímicos. Como destaque, estão os genótipos PW1, PW2, PW5, BB3 e as cultivares Bluegem, Briteblue, Climax e Delite. Assim, os genótipos de mirtileiro do presente estudo, são considerados tão promissores para a indústria alimentícia e também para o consumo in natura quanto as cultivares comerciais já consolidadas no mercado da fruticultura, tendo em vista a excelente composição fenólica presente nestes pequenos frutos.

First Report of Mulberry Rust Caused by Cerotelium fici on Morus nigra in Brazil.

Leaf rust caused by Cerotelium fici (Cast.) Arth. is the main disease affecting Moraceae family plants, such as Ficus and Morus species (Galleti and Rezende 2016; Srikantaswamy et al. 2006). In August 2020, rust symptoms were observed in 100% of mulberry (Morus nigra L.) trees in an experimental orchard (Piracicaba, SP, Brazil; 22°42'28"S, 47°37'42"W). Mulberry leaves with high rust severity became yellowish and fell-off prematurely. Pustules were light brown with yellowish halo and presented mean size of 0.9 mm2. Uredinial paraphyses (n = 50) measured 42.2 ± 0.67 µm long with wall uniformly ca 0.6-1.1 µm thick. Urediniospores were brownish, echinulate, globoid to broadly ellipsoid, and measured 27.1 ± 0.29 × 21.0 ± 0.27 µm with a wall thickness of 0.6 ± 0.01 µm (n = 100). The morphology of the urediniospores observed in this study was similar to that reported in the literature for C. fici on Morus alba and Ficus spp. (Gupta et al. 1994; McKenzie 1986; Hennen et al. 2005). We used a low-coverage genome-skimming approach to retrieve genetic information of the rRNA cluster and the mtDNA. Genomic DNA was extracted from 3-4 mg of stored urediniospores at -80 °C, macerated in liquid nitrogen, using a modified cetyl trimethylammonium bromide extraction procedure (Lo Piccolo et al. 2012), and sequenced with 150-bp paired-end reads on Illumina NovaSeq 6000 System. Raw data, (45,761,957 X 2 reads) were assembled with SPAdes v3.15.1 (Bankevich et al., 2012) and the output used to create a custom BLAST database. Loci used for the phylogenetic analyses were identified by BLASTn using, as a query, sequences of C. fici from Ficus sp. from Australia publicly available: Accession No. MH047210.1 for the rRNA and MW036502.1 for COX3. The retrieved sequences were deposited in GenBank under accession numbers OM296992 and OP797407 for the partial rRNA cluster and COX3, respectively. The Bayesian inference phylogenetic analysis of the three concatenate loci (18S, 28S, and COX3) revealed that the isolate obtained in this study (MN1) was clustered in a well-supported clade with C. fici type species. Pathogenicity tests were conducted using mulberry potted plants under greenhouse conditions (25 ± 5 °C). The urediniospores suspension (5 × 104 urediniospores ml-1) with 0.05% Tween 20 was sprayed with an airbrush on fully expanded leaves until run-off. As a control, mulberry plants were sprayed with distilled water and kept under the same conditions. Inoculated and mock-inoculated plants were kept in a dark moist chamber at 23 °C (± 2 °C) for 24 h. After this period, plants were moved to the greenhouse. The experimental design was completely randomized with five replicates, each replicate consisted of one potted plant and the experiment was performed twice. At 12 days post-inoculation, all inoculated plants showed rust symptoms identical to those observed in the field, whereas control plants had no symptoms. The first symptoms were small pustules on the abaxial surface of fully expanded leaves. Small chlorotic lesions were observed on the adaxial leaf surface, which evolved into necrotic lesions. The pathogen was re-inoculated into potted plants, where it was maintained through monthly inoculations. To our knowledge, this is the first report of mulberry rust on M. nigra in Brazil. As mulberry leaves are the only natural food for silkworm (Bombyx mori L.), rust poses a significant threat to the sericulture industry because the disease can decrease production and quality of mulberry foliage.

First report of Colletotrichum fioriniae causing anthracnose fruit rot on Vaccinium corymbosum in Chile.

Vaccinium corymbosum L. is the most cultivated blueberry species in Chile. Chilean fruits typically take up to 50 days to reach oversea markets; therefore, controlling post-harvest pathogens is of outmost importance to maintain international food safety and quality standards. In February 2019, the Microbial Genetic Resources Bank at INIA received fruits of V. corymbosum cv. 'Brigitta Blue' from Mariquina (-39.567869, -72.992461), located in the southern Chilean blueberry production zone, for post-harvest disease diagnosis. Asymptomatic fruits were incubated in moist-chambers at 25 °C with light/darkness cycles of 12 h. After 5 d, some fruits showed sunken areas and small surface wounds that exudated orange masses of conidia; under the epidermis, gray acervuli were also detected. After 15d, these fruits became dehydrated, mummified, and covered by mycelia, all characteristic symptoms of anthracnose (Wharton and Schilder 2008). In Chile, Colletotrichum gloeosporioides has, thus far, been the only causal agent of anthracnose reported in blueberry (Lara et al. 2003). Conidia exudated from the diseased fruit were inoculated on potato-dextrose agar (PDA) and incubated at 25 °C for 7 d. The resulting colony was predominantly cottony with gray aerial mycelium, displaying masses of pale orange conidia; on the reverse side, the colony was a pink-reddish color. Under a microscope, conidia were hyaline, fusiform to elliptic in shape, and displaying guttulate of 12.2±1.2 × 4.17±0.3 µm (n=30), characteristics coinciding with those described for Colletotrichum fioriniae (Pennycook 2017; Shivas and Tan 2009) (Supplementary Figure 1). The isolate was deposited in the Chilean Collection of Microbial Genetic Resources (CChRGM) as RGM 3330. Genomic DNA extraction of RGM 3330 and phylogenetic analyses were carried out according to Cisterna-Oyarce et al. (2022). A multi-locus sequencing analysis was carried out using five genetic markers. The internal transcribed spacer (ITS), glyceraldehyde 3-phosphate dehydrogenase (gapdh), actin (act), and chitin synthase 1 (chs-1) were PCR-amplified following Damm et al. (2012) and -tubulin (tub) following Glass and Donaldson (1995). Sequences were deposited in GenBank (ON364141 for ITS and ON369167-70 for tub, act, chs-1, and gapdh, respectively) (Sayers et al. 2021). A BLAST analysis carried out in SequenceServer (Priyam et al. 2019), using a custom database of sequences retrieved from Damm et al. (2012) and Liu et al. (2020), showed that all genetic markers were 100% identical to those of C. fioriniae CBS 128517T (ITS (540/540 identities), gapdh (249/249), act (245/245), and chs-1 (274/274)), except for tub, which shared 99.8% of its identities (416/417) with this species. Maximum likelihood phylogenetic estimation clustered RGM 3330 with C. fioriniae strains CBS 128517T and CBS 126526 with 100% bootstrap support (Supplementary Figure 1). Koch's postulates were carried out with asymptomatic fruits of V. corymbosum cv. 'Brigitta Blue'. Prior to inoculation, fruits were surface-sterilized for 10 s in 70% ethanol, 3 s in 1% NaOCl, 10 s in 70% ethanol, rinsed three times with sterile distilled water, and subsequently placed in moist-chambers. Two groups of three repetitions of 20 fruits each were sprayed with 9 × 106 conidia/mL of RGM 3330 for the first group and with sterile distilled water for the control. After 5 d at 25 °C with light/darkness cycles of 12 h, only fruits sprayed with the conidial solution developed symptoms of anthracnose and the re-isolated fungi were identical in morphology to RGM 3330. This is the first report of C. fioriniae in blueberry in Chile. References Cisterna-Oyarce, V., Carrasco-Fernández, J., Castro, J. F., Santelices, C., Muñoz-Reyes, V., Millas, P., Buddie, A. G., and France, A. 2022. Gnomoniopsis smithogilvyi: identification, characterization and incidence of the main pathogen causing brown rot in postharvest sweet chestnut fruits (Castanea sativa) in Chile. Australasian Plant Disease Notes 17:2. Damm, U., Cannon, P. F., Woudenberg, J. H., and Crous, P. W. 2012. The Colletotrichum acutatum species complex. Stud. Mycol. 73:37-113. Glass, N. L., and Donaldson, G. C. 1995. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl. Environ. Microbiol. 61:1323-1330. Lara, O., Velazquez, C. G., and Ascencio, C. 2003. Colletotrichum gloeosporiodes in blueberry fruit. in: XIII Congreso de Fitopatología. Liu, X., Zheng, X., Khaskheli, M. I., Sun, X., Chang, X., and Gong, G. 2020. Identification of Colletotrichum species associated with blueberry anthracnose in Sichuan, China. Pathogens 9:718. Pennycook, S. 2017. Colletotrichum fioriniae comb. & stat. nov., resolving a nomenclatural muddle. Mycotaxon 132:149-152. Priyam, A., Woodcroft, B. J., Rai, V., Moghul, I., Munagala, A., Ter, F., Chowdhary, H., Pieniak, I., Maynard, L. J., Gibbins, M. A., Moon, H., Davis-Richardson, A., Uludag, M., Watson-Haigh, N. S., Challis, R., Nakamura, H., Favreau, E., Gómez, E. A., Pluskal, T., Leonard, G., Rumpf, W., and Wurm, Y. 2019. Sequenceserver: a modern graphical user interface for custom BLAST databases. Mol. Biol. Evol. 36:2922-2924. Sayers, E. W., Cavanaugh, M., Clark, K., Pruitt, K. D., Schoch, C. L., Sherry, S. T., and Karsch-Mizrachi, I. 2021. GenBank. Nucleic Acids Res. 49:D92-D96. Shivas, R. G., and Tan, Y. P. 2009. A taxonomic re-assessment of Colletotrichum acutatum, introducing C. fioriniae comb. et stat. nov. and C. simmondsii sp. nov. Fungal Divers. 39:111-122. Wharton, P., and Schilder, A. 2008. Novel infection strategies of Colletotrichum acutatum on ripe blueberry fruit. Plant Pathol. 57:122-134. Supplementary material Supplementary Figure 1: Isolation and identification of Colletotrichum fioriniae RGM 3330 from blueberry fruits cv. 'Brigitta Blue' from Chile. (A) A fruit showing anthracnose; (B) colony of Colletotrichum fioriniae RGM 3330 growing on PDA; (C) microscopic observation of the conidia (100x magnification; bar=10 µm); (D) phylogenetic tree resulting from a maximum likelihood analysis of combined sequence data from ITS, act, chs-1, gapdh, and tub regions for Colletotrichum acutatum species complex, number in the nodes represent ultrafast bootstrap values.

First Report of Colletotrichum chrysophilum Causing Anthracnose on Blueberry in Brazil.

Highbush (Vaccinium corymbosum L.) and rabbiteye (V. ashei R.) blueberry are the most important export small fruit crops in southern Brazil. Anthracnose has been considered one of the most destructive disease and exclusively associated with C. karstii in Brazil (Rios et al. 2014). In November 2019, severe anthracnose symptoms including leaf spots but particularly twig blights and fruit rots were observed on all blueberry plants (V. ashei) in one organic orchard in Santa Catarina state, Brazil (27º43'48.96"S, 49º0'57.79"W). Four isolates were obtained from necrotic lesions and monosporic cultures were grown on potato dextrose agar at 25°C and with a 12 h photoperiod under near ultra violet light. After 15 days, colonies showed upper surface color varying from grayish-white to pale-orange and the reverse side pale-orange. Conidia were hyaline, cylindrical with rounded ends, and their length and width ranged from 9.5 to 15.5 µm (x ̅=11.8) and 6.5 to 3.5 µm (x ̅=4.9), respectively. The isolates were identified by multilocus phylogenetic analyses using nucleotide sequences of actin (ACT), ß-tubulin (TUB2), calmodulin (CAL), glyceraldehyde-3-phosphate-dehydrogenase (GAPDH), glutamine synthetase (GS), internal transcribed spacer (ITS) and the intergenic spacer between DNA lyase and the mating-type locus MAT1-2-1 (ApMAT). Nucleotide sequences exhibited from 95 to 100% sequence identity to Colletotrichum chrysophilum ex-type (CMM4268) and were deposited in GenBank database (MW868219 to MW868222, MW868211 to MW868214, MW868215 to MW868218, MW868223 to MW868226, MW868202 to MW868205, MW793353 to MW793356, and MW868207 to MW868210). C. chrysophilum belongs to the C. gloeosporioides species complex and was previously described as C. ignotum in banana and other tropical fruits in Brazil (Vieira et al. 2017; Veloso et al. 2018). In addition, this species was recently reported on apple fruit in New York, USA (Khodadadi et al. 2020). To confirm pathogenicity, one-year-old blueberry plants were inoculated by spraying a suspension of 1×106 conidia/ml, incubated in a moist chamber in the dark for 48 h and then kept in the greenhouse. Plants sprayed with sterile distilled water served as control. Additionally, fruits were immersed for 2 min in a conidial suspension (1×106 conidia/ml) and incubated at 25°C and 12 h photoperiod for 20 days. Inoculated plants exhibited first symptoms in twigs at 10 days after inoculation (dai). Infected twigs showed initially dark brown spots that coalesced and became necrotic. On leaves, reddish-brown lesions with less than 2 mm appeared at low intensity at 15 dai. On fruits, sunken areas associated with an abundant orange mucilaginous mass of acervuli and conidia were seen at 7 dai. Symptoms on plants were identical to those observed under field conditions, and the pathogen was re-isolated from lesions fulfilling Koch's postulates. To the best knowledge, this is the first report of C. chrysophilum causing anthracnose on blueberries in Brazil. The identification of this species causing blueberry anthracnose is crucial to improve the disease control strategies and resistance breeding.

Nutraceutical fruit characterization, nutritional aspects and sensory analysis of dovyalis jams / Caracterização de um fruto nutracêutico, seu aspecto nutricional e análise sensorial de geleia

The objective of this study is to evaluate the composition, nutritional aspects and sensory analysis of the dovyalis jam. Dovyalis fruits were harvested from 4-year-old plants in the orchard of the Unioeste Experimental Farm. They were collected in the morning in polyethylene boxes, when they were ripe and with a purple coloration from the epicarp, being immediately transported to the Laboratory to prepare the jams. After fruits arrival, they were selected by size, color and weight and then sanitized in running water and the cups of the fruits were removed. The sensory evaluations of the jam samples were performed in a randomized fashion, with 30 volunteer tasters. The tasters were previously advised to evaluate the acceptance of the jams and their purchase intentions. Fruits in natura and dovyalis jam are sources of vitamin C. Dovyalis fruits can be used in the processing, due to its acidity. The jam prepared with dovyalis fruits without epicarp and endocarp, and less sugar, was more appreciated by the tasters.(AU)

Objetivou-se com o presente trabalho avaliar a composição, aspectos nutricionais e análise sensorial da geleia de dovyalis. Frutos de dovyalis foram colhidos de plantas de quatro anos, no pomar da Fazenda Experimental da Unioeste. Estes, foram coletados de manhã, em caixas de polietileno, quando encontravam-se maduros e com coloração arroxeada do epicarpo, sendo imediatamente após, transportados ao laboratório, para o preparo das geleias. Após a chegada dos frutos, estes foram selecionados por tamanho, coloração e peso e, em seguida, higienizados em água corrente e retirados os cálices dos frutos. As avaliações sensoriais das amostras de geleia foram realizadas de forma casualizada, com 30 provadores voluntários. Os provadores foram previamente orientados a avaliarem a aceitação das geleias e suas intenções de compra. Frutos in natura e geleia de dovyalis são fontes de vitamina C. Frutos de dovyalis podem ser usados no processamento, devido sua acidez. A geleia preparada com frutos de dovyalis sem epicarpo e endocarpo, e menor quantidade de açúcar, foi mais apreciada pelos provadores.(AU)

Nutraceutical fruit characterization, nutritional aspects and sensory analysis of dovyalis jams / Caracterização de um fruto nutracêutico, seu aspecto nutricional e análise sensorial de geleia

ABSTRACT: The objective of this study is to evaluate the composition, nutritional aspects and sensory analysis of the dovyalis jam. Dovyalis fruits were harvested from 4-year-old plants in the orchard of the Unioeste Experimental Farm. They were collected in the morning in polyethylene boxes, when they were ripe and with a purple coloration from the epicarp, being immediately transported to the Laboratory to prepare the jams. After fruits arrival, they were selected by size, color and weight and then sanitized in running water and the cups of the fruits were removed. The sensory evaluations of the jam samples were performed in a randomized fashion, with 30 volunteer tasters. The tasters were previously advised to evaluate the acceptance of the jams and their purchase intentions. Fruits in natura and dovyalis jam are sources of vitamin C. Dovyalis fruits can be used in the processing, due to its acidity. The jam prepared with dovyalis fruits without epicarp and endocarp, and less sugar, was more appreciated by the tasters.

RESUMO: Objetivou-se com o presente trabalho avaliar a composição, aspectos nutricionais e análise sensorial da geleia de dovyalis. Frutos de dovyalis foram colhidos de plantas de quatro anos, no pomar da Fazenda Experimental da Unioeste. Estes, foram coletados de manhã, em caixas de polietileno, quando encontravam-se maduros e com coloração arroxeada do epicarpo, sendo imediatamente após, transportados ao laboratório, para o preparo das geleias. Após a chegada dos frutos, estes foram selecionados por tamanho, coloração e peso e, em seguida, higienizados em água corrente e retirados os cálices dos frutos. As avaliações sensoriais das amostras de geleia foram realizadas de forma casualizada, com 30 provadores voluntários. Os provadores foram previamente orientados a avaliarem a aceitação das geleias e suas intenções de compra. Frutos in natura e geleia de dovyalis são fontes de vitamina C. Frutos de dovyalis podem ser usados no processamento, devido sua acidez. A geleia preparada com frutos de dovyalis sem epicarpo e endocarpo, e menor quantidade de açúcar, foi mais apreciada pelos provadores.

Yield and quality of primocane-fruiting raspberry grown under plastic cover in southern Brazil

Raspberry (Rubus idaeus L.) is an economically important crop and its cultivation has been expanded across temperate and subtropical regions of Brazil. Growing raspberries under plastic cover has becoming more common worldwide. This study investigated the effect of plastic cover on productive and morphological performance of primocane-fruiting raspberry cultivars, in southern Brazil. The experiment was arranged in a 3 × 2 × 2 factorial design, with three raspberry cultivars, ‘Heritage; ‘Fallgold and ‘Alemãzinha; two cultivation systems, presence and absence of plastic cover; and two production cycles, fall and spring. We measured days from transplanting to flowering and harvesting. Plants were evaluated for dry mass of pruning, plant height, stem diameter, plant leaf area, fruit production, number of fruits, fruit weight, fruit longitudinal and transversal diameter, and harvesting period. Plastic cover reduced incident radiation on the canopy by 17 %. Cultivar ‘Heritage is early ripening in the fall cycle. The management under plastic cover prolonged the harvesting period of raspberries in the fall productive cycle for the cultivar ‘Heritage and, in spring, for cultivars ‘Alemãzinha and ‘Fallgold. In the spring cycle, plants increased their leaf area, fruit production and number of fruits. The cultivar ‘Alemãzinha performed better in the spring cycle when managed under plastic cover and was more productive, with a longer productive cycle and larger fruits.(AU)

Yield and quality of primocane-fruiting raspberry grown under plastic cover in southern Brazil

Raspberry (Rubus idaeus L.) is an economically important crop and its cultivation has been expanded across temperate and subtropical regions of Brazil. Growing raspberries under plastic cover has becoming more common worldwide. This study investigated the effect of plastic cover on productive and morphological performance of primocane-fruiting raspberry cultivars, in southern Brazil. The experiment was arranged in a 3 × 2 × 2 factorial design, with three raspberry cultivars, ‘Heritage’; ‘Fallgold’ and ‘Alemãzinha’; two cultivation systems, presence and absence of plastic cover; and two production cycles, fall and spring. We measured days from transplanting to flowering and harvesting. Plants were evaluated for dry mass of pruning, plant height, stem diameter, plant leaf area, fruit production, number of fruits, fruit weight, fruit longitudinal and transversal diameter, and harvesting period. Plastic cover reduced incident radiation on the canopy by 17 %. Cultivar ‘Heritage’ is early ripening in the fall cycle. The management under plastic cover prolonged the harvesting period of raspberries in the fall productive cycle for the cultivar ‘Heritage’ and, in spring, for cultivars ‘Alemãzinha’ and ‘Fallgold’. In the spring cycle, plants increased their leaf area, fruit production and number of fruits. The cultivar ‘Alemãzinha’ performed better in the spring cycle when managed under plastic cover and was more productive, with a longer productive cycle and larger fruits.

Morphological and Molecular Identification of Two Florida Populations of Foliar Nematodes (Aphelenchoides spp.) Isolated From Strawberry With the Description of Aphelenchoides pseudogoodeyi sp. n. (Nematoda: Aphelenchoididae) and Notes on Their Bionomics.

Two Florida populations of foliar nematodes were collected from strawberries originating from Cashiers, North Carolina (USA) located west from Willard, the type locality of Aphelenchoides besseyi. Both nematodes were cultured on Monilinia fructicola and identified using morphological characteristics and molecular assays as Aphelenchoides besseyi and Aphelenchoides pseudogoodeyi sp. n., a herein described new species related to Aphelenchoides goodeyi belonging to the Group of Aphelenchoides exhibiting stellate tails. The morphological and biological characters of Florida A. besseyi fit those of the original description of this species. A. pseudogoodeyi sp. n., which was initially misidentified as Aphelenchoides fujianensis, differed from the type population of the latter species from China because it was without males, and females lacked a functional spermatheca, whereas type A. fujianensis is an amphimictic species. Phylogenetic analyses using near full-length 18S ribosomal RNA (rRNA), the D2-D3 expansion fragments of 28S rRNA, and partial COI gene sequences indicated that A. besseyi is a species complex. A. pseudogoodeyi sp. n. grouped in different clades from those of the type A. fujianensis, instead merging with populations identified of 'A. fujianensis' from Brazil and other countries, suggesting that the latter are conspecific and incorrectly identified. The Florida A. besseyi infected strawberry and gerbera daisy, but not soybean and alfalfa. A. pseudogoodeyi sp. n. is mainly mycetophagous. Localized inoculation of 300 specimens applied with filter paper adhering to the blade of the soybean leaves resulted in nematode penetration into the mesophyll with subsequent development of lesions limited to the inoculated area of the blade.

Characterization of blueberry cultivar ‘climax’ / Caracterização da cultivar ‘climax’ de mirtileiro

The objective of this work was to characterize the blueberry (Vaccinium spp.) cultivar Climax, Rabbiteye group (Vaccinium ashei Reade). In order to achieve that, a survey with blueberry plants (cultivar Climax), in full production, was conducted during the 2012/13 and 2013/14 seasons in the mesoregion of Pelotas, Rio Grande do Sul State, Brazil. Data regarding the distribution of flower and vegetative buds on branches of two lengths (long and short) was collected. Another evaluation, considering three positions of flower buds in the branch (apical, medial and basal), the number of flower primordia, open flowers and fruits formed was performed. Data were subject to ANOVA and, when significant, the averages were compared using either the F or Tukey’s HSD test (p ≤ 0.05). The results demonstrate the presence of flower buds intercalated with vegetative ones. The total number of flower and vegetative buds, flower primordia, flowers and fruits was influenced by the length of the branch. Likewise, the position of the bud on the branch affected the results, with a greater number of flower primordia on the basal, a greater number of flowers on the medial and a greater number of fruits on the apical portions of the branches.

Objetivou-se realizar a caracterização do mirtileiro (Vaccinium spp.), cultivar Clímax, grupo Rabbiteye (Vaccinium ashei Reade). Para tanto, foi conduzida a pesquisa com plantas da cultivar Clímax, em plena produção, durante as safras de 2012/13 e 2013/14. Foram coletados dados referentes à distribuição das gemas floríferas e vegetativas nos ramos de dois comprimentos (longos e curtos). Bem como, sob estes, foi avaliado, considerando-se três posições de gemas floríferas no ramo (apical, mediana e basal), o número de primórdios florais, flores abertas e frutos formados. Os dados foram submetidos à análise de variância e quando houve efeito significativo suas médias foram comparadas pelo teste de F e/ou Tukey (p ≤ 0,05). Os resultados demonstraram a presença de gemas floríferas intercaladas às vegetativas. O número total de gemas floríferas e vegetativas, de primórdios florais, flores e frutos foi influenciado pelo comprimento do ramo. Da mesma forma, a posição da gema no ramo, influenciou os resultados, sendo observado maior número de primórdios florais na gema basal, maior número de flores na porção mediana e maior número de frutos na apical.

Characterization of blueberry cultivar ‘climax / Caracterização da cultivar ‘climax de mirtileiro

The objective of this work was to characterize the blueberry (Vaccinium spp.) cultivar Climax, Rabbiteye group (Vaccinium ashei Reade). In order to achieve that, a survey with blueberry plants (cultivar Climax), in full production, was conducted during the 2012/13 and 2013/14 seasons in the mesoregion of Pelotas, Rio Grande do Sul State, Brazil. Data regarding the distribution of flower and vegetative buds on branches of two lengths (long and short) was collected. Another evaluation, considering three positions of flower buds in the branch (apical, medial and basal), the number of flower primordia, open flowers and fruits formed was performed. Data were subject to ANOVA and, when significant, the averages were compared using either the F or Tukeys HSD test (p ≤ 0.05). The results demonstrate the presence of flower buds intercalated with vegetative ones. The total number of flower and vegetative buds, flower primordia, flowers and fruits was influenced by the length of the branch. Likewise, the position of the bud on the branch affected the results, with a greater number of flower primordia on the basal, a greater number of flowers on the medial and a greater number of fruits on the apical portions of the branches.(AU)

Objetivou-se realizar a caracterização do mirtileiro (Vaccinium spp.), cultivar Clímax, grupo Rabbiteye (Vaccinium ashei Reade). Para tanto, foi conduzida a pesquisa com plantas da cultivar Clímax, em plena produção, durante as safras de 2012/13 e 2013/14. Foram coletados dados referentes à distribuição das gemas floríferas e vegetativas nos ramos de dois comprimentos (longos e curtos). Bem como, sob estes, foi avaliado, considerando-se três posições de gemas floríferas no ramo (apical, mediana e basal), o número de primórdios florais, flores abertas e frutos formados. Os dados foram submetidos à análise de variância e quando houve efeito significativo suas médias foram comparadas pelo teste de F e/ou Tukey (p ≤ 0,05). Os resultados demonstraram a presença de gemas floríferas intercaladas às vegetativas. O número total de gemas floríferas e vegetativas, de primórdios florais, flores e frutos foi influenciado pelo comprimento do ramo. Da mesma forma, a posição da gema no ramo, influenciou os resultados, sendo observado maior número de primórdios florais na gema basal, maior número de flores na porção mediana e maior número de frutos na apical.(AU)

Germinação e crescimento inicial de mudas de espécies não convencionais de fisális em diferentes substratos e ambientes / Germination and initial growth of non-conventional species of physalis on different substrates and environments

A utilização de substratos e ambientes é importante fator a ser analisado na determinação da qualidade de germinação e crescimento de mudas de espécies de fisális. Diante do exposto, objetivou-se com o presente trabalho, avaliar a germinação e o crescimento inicial de duas espécies não convencionais de fisális, submetidas a diferentes ambientes e substratos. Conduziu-se dois experimentos simultâneos no período de março a maio/2014, na Unioeste, PR. O delineamento experimental utilizado no primeiro experimento foi blocos casualizados, fatorial 2x3 (2 espécies de fisális: Physalis minima, Physalis ixocarpax 3 ambientes: ambiente protegido com cobertura plástica de 150 µm; telado com 50% de sombreamento e pleno sol), contendo 3 repetições e 50 sementes por parcela experimental. O delineamento do segundo experimento foi blocos casualizados, fatorial 2x4 (2 espécies de fisális: Physalis minima, Physalis ixocarpax 4 substratos: vermiculita expandida de textura média + Latossolo; Húmus Fértil® + Latossolo; areia lavada de textura fina + Latossolo e Húmus Fértil® + vermiculita expandida de textura média, na proporção 1:1 v:v), contendo 3 repetições e 32 sementes por parcela experimental. Monitorou-se os experimentos por 22 dias, para obtenção do índice de velocidade de emergência. Aos 60 dias após a semeadura avaliou-se a altura das plântulas, diâmetro do caule, número de folhas, biomassa fresca e seca das plântulas. Estufa agrícola e telado propiciam índice de velocidade de emergência de sementes de Physalis ixocarpa e Physalis mínima, sem distinção entre esses ambientes. Mudas de P. ixocarpa devem ser produzidas em estufa, utilizando-se como mistura de substrato, Húmus Fértil® + vermiculita expandida de textura média, ou Latossolo + vermiculita. Mudas de P. minima devem ser produzidas em mistura de Húmus Fértil® + vermiculita, sob estufa ou telado.(AU)

The use of substrates and environments is an important factor to be considered in determining the quality of germination and seedling growing of species of physalis. Given the above, the objective with this study was to evaluate the germination and early crowing of two non-conventional species of physalis, subjected to different environments and substrates. Were conducted two simultaneous experiments in the periodfrom March to May / 2014 in Unioeste, PR. The experimental design used in the first experiment was a randomized, 2x3 factorial (2 physalis species: Physalis minima, Physalis ixocarpa x 3 environments: protect environment with plastic cover of 150 µm, greenhouse with 50% of shading and full sun) blocks containing 3 replicates and 50 seeds per experimental plot. The design of the second experiment was randomized, 2x4 factorial (2 physalis species: Physalis minima, Physalis ixocarpa x 4 substrates:expanded vermiculite of medium texture + latossol, Húmus Fértil® + latossol, washed sand of fine texture +latossol and Humus Fértil® + expanded vermiculite of medium texture, in proportion 1:1 v:v) blocks containing three replicates of 32 seeds per experimental plot. Was monitored by 22 days the experiments to obtain the emergence speed index. 60 days after the sowing was evaluated seedling height, stem diameter, number of leaves, fresh and dry biomass of seedlings. We conclude that, agricultural greenhouse and greenhouse of shade net providing emergence speed index of Physalis minima and Physalis Ixocarpa without distinction between these environments. Seedlings of P. ixocarpa should be produced in agricultural greenhouse, using as substrate mix of Humus fértil® commercial substrate + expanded vermiculite or latosol + vermiculite. Seedlings of P. minima must be produced in a mixture of Humus Fértil® + vermiculite under agricultural greenhouse with or under shade net.(AU)

Germinação e crescimento inicial de mudas de espécies não convencionais de fisális em diferentes substratos e ambientes / Germination and initial growth of non-conventional species of physalis on different substrates and environments

A utilização de substratos e ambientes é importante fator a ser analisado na determinação da qualidade de germinação e crescimento de mudas de espécies de fisális. Diante do exposto, objetivou-se com o presente trabalho, avaliar a germinação e o crescimento inicial de duas espécies não convencionais de fisális, submetidas a diferentes ambientes e substratos. Conduziu-se dois experimentos simultâneos no período de março a maio/2014, na Unioeste, PR. O delineamento experimental utilizado no primeiro experimento foi blocos casualizados, fatorial 2x3 (2 espécies de fisális: Physalis minima, Physalis ixocarpax 3 ambientes: ambiente protegido com cobertura plástica de 150 µm; telado com 50% de sombreamento e pleno sol), contendo 3 repetições e 50 sementes por parcela experimental. O delineamento do segundo experimento foi blocos casualizados, fatorial 2x4 (2 espécies de fisális: Physalis minima, Physalis ixocarpax 4 substratos: vermiculita expandida de textura média + Latossolo; Húmus Fértil® + Latossolo; areia lavada de textura fina + Latossolo e Húmus Fértil® + vermiculita expandida de textura média, na proporção 1:1 v:v), contendo 3 repetições e 32 sementes por parcela experimental. Monitorou-se os experimentos por 22 dias, para obtenção do índice de velocidade de emergência. Aos 60 dias após a semeadura avaliou-se a altura das plântulas, diâmetro do caule, número de folhas, biomassa fresca e seca das plântulas. Estufa agrícola e telado propiciam índice de velocidade de emergência de sementes de Physalis ixocarpa e Physalis mínima, sem distinção entre esses ambientes. Mudas de P. ixocarpa devem ser produzidas em estufa, utilizando-se como mistura de substrato, Húmus Fértil® + vermiculita expandida de textura média, ou Latossolo + vermiculita. Mudas de P. minima devem ser produzidas em mistura de Húmus Fértil® + vermiculita, sob estufa ou telado.

The use of substrates and environments is an important factor to be considered in determining the quality of germination and seedling growing of species of physalis. Given the above, the objective with this study was to evaluate the germination and early crowing of two non-conventional species of physalis, subjected to different environments and substrates. Were conducted two simultaneous experiments in the periodfrom March to May / 2014 in Unioeste, PR. The experimental design used in the first experiment was a randomized, 2x3 factorial (2 physalis species: Physalis minima, Physalis ixocarpa x 3 environments: protect environment with plastic cover of 150 µm, greenhouse with 50% of shading and full sun) blocks containing 3 replicates and 50 seeds per experimental plot. The design of the second experiment was randomized, 2x4 factorial (2 physalis species: Physalis minima, Physalis ixocarpa x 4 substrates:expanded vermiculite of medium texture + latossol, Húmus Fértil® + latossol, washed sand of fine texture +latossol and Humus Fértil® + expanded vermiculite of medium texture, in proportion 1:1 v:v) blocks containing three replicates of 32 seeds per experimental plot. Was monitored by 22 days the experiments to obtain the emergence speed index. 60 days after the sowing was evaluated seedling height, stem diameter, number of leaves, fresh and dry biomass of seedlings. We conclude that, agricultural greenhouse and greenhouse of shade net providing emergence speed index of Physalis minima and Physalis Ixocarpa without distinction between these environments. Seedlings of P. ixocarpa should be produced in agricultural greenhouse, using as substrate mix of Humus fértil® commercial substrate + expanded vermiculite or latosol + vermiculite. Seedlings of P. minima must be produced in a mixture of Humus Fértil® + vermiculite under agricultural greenhouse with or under shade net.

Performance of blueberry cultivars under mild winter conditions / Desempenho de cultivares de mirtileiro em condição de inverno ameno

Evaluation of yield performance is important to find the most adapted blueberry cultivars in a particular region. This research aimed to evaluate the flowering and hasvesting periods, the production per plant, and fruit quality of eight rabbiteye blueberry cultivars (Aliceblue, Bluebelle, Bluegem, Briteblue, Climax, Delite, Powderblue, and Woodard) and two highbush blueberries (Georgiagem and ONeal), in mild winter conditions in Pinhais-PR. Flowering and harvesting periods, production, berry weight, size, pH, soluble solids, titratable acidity, ratio and color of the fruits were evaluated in the 2013/2014 and 2014/2015 growing seasons, when the plants had two and three years old, respectively. Cultivars flowered from August to September, and harvest was concentrated in November and December. Berry weight, size, pH, soluble solids and acidity varied among the cultivars. The average ratios of 14.97 and 13.39 for each crop proved that the cultivars have good fruit quality. There was little variation in fruit color in the two years evaluated. Blueberry cultivars showed the staining characteristics and physical and chemical attributes of quality compatible to blueberry from other traditional regions. Under mild winter conditions, young plants of rabbiteye blueberry cultivars, Climax, Delite, Bluegem and Powderblue, are the most productive, while the highbusch cultivars bear few fruits.(AU)

A avaliação do desempenho produtivo é importante para encontrar as cultivares de mirtileiro mais adaptadas à uma determinada região. Este trabalho teve como objetivo avaliar o período de floração e de colheita, a produção por planta e a qualidade de frutos de oito cultivares de mirtileiro do grupo rabbiteye (Aliceblue, Bluebelle, Bluegem, Briteblue, Climax, Delite, Powderblue e Woodard) e duas do grupo highbush (Georgiagem e ONeal) em condição de inverno ameno em Pinhais-PR. Foram avaliadas as datas de início e final de floração, início e final de colheita, produção por planta, massa, tamanho de fruto, pH, teor de sólidos solúveis, acidez titulável, ratio e coloração nos ciclos produtivos de 2013/2014 e 2014/2015, quando as plantas possuíam dois e três anos, respectivamente.As cultivares estudadas apresentaram floração nos meses de agosto e setembro, concentrando a colheita nos meses de novembro e dezembro. Houve diferenças entre as cultivares quanto a massa do fruto, tamanho, pH, teor de sólidos solúveis e a acidez. O ratio médio de 14,97 e 13,39 obtido em cada safra provou que as cultivares tem boa qualidade de frutos. A coloração dos frutos apresentou poucas variações nos dois anos avaliados. O mirtilo das cultivares avaliadas apresentou características de coloração e atributos físicos e químicos de qualidade compatível com o mirtilo de outras regiões tradicionalmente produtoras. Em região com inverno ameno, plantas jovens das cultivares Climax, Delite, Bluegem e Powderblue, do grupo rabbiteye, são as mais produtivas, enquanto as cultivares do grupo highbush produzem poucos frutos.(AU)

Initial development of physalis species under growing environments / Desenvolvimento inicial de espécies de fisális submetidas a ambientes de cultivo

The environment can be a limiting factor for development seedlings of Physalis species. Thus, aiming this present study was evaluate the initial development of Physalis species subjected to cultivation environments. The experimental design was a randomized block design, factorial 3 x 3 (three Physalis species being Physalis peruviana, P. angulata and P. pubescens x three environments, stove - EST, plastic cover with 150 micron; shading - SBT, with 75% permeability and light, full sun - PLS, in a fully open), with four repetitions, conducted between february and may/2012, in the Station of Horticulture and Biological Control Mário César Lopes, University of Paraná State, Brazil. During the experiment, was monitored the emergence speed rate of seedlings in the initial 30 days. The 76 days after germination the sowing to leaf area, dry biomass leaves, stems and roots, as well as the number leaves, plant height and stem diameter. The environment SBT provides higher emergence rate of Physalisspecies seedlings studied. Environments EST and SBT were similar in the Physalisdevelopment early and can be used in the production of these species seedlings. Is not advisable for the initial development for of Physalis seedlings occur in PLS.

Initial development of physalis species under growing environments / Desenvolvimento inicial de espécies de fisális submetidas a ambientes de cultivo

The environment can be a limiting factor for development seedlings of Physalis species. Thus, aiming this present study was evaluate the initial development of Physalis species subjected to cultivation environments. The experimental design was a randomized block design, factorial 3 x 3 (three Physalis species being Physalis peruviana, P. angulata and P. pubescens x three environments, stove - EST, plastic cover with 150 micron; shading - SBT, with 75% permeability and light, full sun - PLS, in a fully open), with four repetitions, conducted between february and may/2012, in the Station of Horticulture and Biological Control Mário César Lopes, University of Paraná State, Brazil. During the experiment, was monitored the emergence speed rate of seedlings in the initial 30 days. The 76 days after germination the sowing to leaf area, dry biomass leaves, stems and roots, as well as the number leaves, plant height and stem diameter. The environment SBT provides higher emergence rate of Physalisspecies seedlings studied. Environments EST and SBT were similar in the Physalisdevelopment early and can be used in the production of these species seedlings. Is not advisable for the initial development for of Physalis seedlings occur in PLS.(AU)

O ambiente pode ser um fator limitante para o desenvolvimento de mudas de espécies de fisális. Diante do exposto, objetivou-se com o presente trabalho avaliar o desenvolvimento inicial de espécies de fisális submetidas a ambientes de cultivo. O delineamento experimental utilizado foi blocos casualizados, em esquema fatorial 3 x 3 (três espécies de fisális, sendo Physalis peruviana, P. angulata e P. pubescens x três ambientes, sendo estufa - EST, com cobertura plástica transparente de 150 micras; sombrite - SBT, com 75% de permeabilidade à luz e, pleno sol - PLS, em local totalmente aberto), contendo quatro repetições, conduzido entre fevereiro e maio de 2012, na Estação de Horticultura e Controle Biológico “Professor Mário César Lopes” (Unioeste), Campus Marechal Cândido Rondon, PR. Durante o experimento, monitorou-se o índice de velocidade de emergência das plântulas nos 30 dias iniciais. Aos 76 dias após a semeadura avaliaram-se a área foliar, biomassa seca das folhas, caules e raízes, bem como o número de folhas, altura das plantas e diâmetro do caule. O ambiente SBT propicia maior taxa de emergência de plântulas das espécies de fisális estudadas. Os ambientes EST e SBT foram similares no desenvolvimento inicial de fisális, podendo ser utilizados na produção de mudas destas espécies. Não é aconselhável que o desenvolvimento inicial de mudas de fisális ocorra em PLS.(AU)

Performance of blueberry cultivars under mild winter conditions / Desempenho de cultivares de mirtileiro em condição de inverno ameno

Evaluation of yield performance is important to find the most adapted blueberry cultivars in a particular region. This research aimed to evaluate the flowering and hasvesting periods, the production per plant, and fruit quality of eight rabbiteye blueberry cultivars (Aliceblue, Bluebelle, Bluegem, Briteblue, Climax, Delite, Powderblue, and Woodard) and two highbush blueberries (Georgiagem and ONeal), in mild winter conditions in Pinhais-PR. Flowering and harvesting periods, production, berry weight, size, pH, soluble solids, titratable acidity, ratio and color of the fruits were evaluated in the 2013/2014 and 2014/2015 growing seasons, when the plants had two and three years old, respectively. Cultivars flowered from August to September, and harvest was concentrated in November and December. Berry weight, size, pH, soluble solids and acidity varied among the cultivars. The average ratios of 14.97 and 13.39 for each crop proved that the cultivars have good fruit quality. There was little variation in fruit color in the two years evaluated. Blueberry cultivars showed the staining characteristics and physical and chemical attributes of quality compatible to blueberry from other traditional regions. Under mild winter conditions, young plants of rabbiteye blueberry cultivars, Climax, Delite, Bluegem and Powderblue, are the most productive, while the highbusch cultivars bear few fruits.

A avaliação do desempenho produtivo é importante para encontrar as cultivares de mirtileiro mais adaptadas à uma determinada região. Este trabalho teve como objetivo avaliar o período de floração e de colheita, a produção por planta e a qualidade de frutos de oito cultivares de mirtileiro do grupo rabbiteye (Aliceblue, Bluebelle, Bluegem, Briteblue, Climax, Delite, Powderblue e Woodard) e duas do grupo highbush (Georgiagem e ONeal) em condição de inverno ameno em Pinhais-PR. Foram avaliadas as datas de início e final de floração, início e final de colheita, produção por planta, massa, tamanho de fruto, pH, teor de sólidos solúveis, acidez titulável, ratio e coloração nos ciclos produtivos de 2013/2014 e 2014/2015, quando as plantas possuíam dois e três anos, respectivamente.As cultivares estudadas apresentaram floração nos meses de agosto e setembro, concentrando a colheita nos meses de novembro e dezembro. Houve diferenças entre as cultivares quanto a massa do fruto, tamanho, pH, teor de sólidos solúveis e a acidez. O ratio médio de 14,97 e 13,39 obtido em cada safra provou que as cultivares tem boa qualidade de frutos. A coloração dos frutos apresentou poucas variações nos dois anos avaliados. O mirtilo das cultivares avaliadas apresentou características de coloração e atributos físicos e químicos de qualidade compatível com o mirtilo de outras regiões tradicionalmente produtoras. Em região com inverno ameno, plantas jovens das cultivares Climax, Delite, Bluegem e Powderblue, do grupo rabbiteye, são as mais produtivas, enquanto as cultivares do grupo highbush produzem poucos frutos.

Initial development of physalis species under growing environments / Desenvolvimento inicial de espécies de fisális submetidas a ambientes de cultivo

The environment can be a limiting factor for development seedlings of Physalis species. Thus, aiming this present study was evaluate the initial development of Physalis species subjected to cultivation environments. The experimental design was a randomized block design, factorial 3 x 3 (three Physalis species being Physalis peruviana, P. angulata and P. pubescens x three environments, stove - EST, plastic cover with 150 micron; shading - SBT, with 75% permeability and light, full sun - PLS, in a fully open), with four repetitions, conducted between february and may/2012, in the Station of Horticulture and Biological Control Mário César Lopes, University of Paraná State, Brazil. During the experiment, was monitored the emergence speed rate of seedlings in the initial 30 days. The 76 days after germination the sowing to leaf area, dry biomass leaves, stems and roots, as well as the number leaves, plant height and stem diameter. The environment SBT provides higher emergence rate of Physalisspecies seedlings studied. Environments EST and SBT were similar in the Physalisdevelopment early and can be used in the production of these species seedlings. Is not advisable for the initial development for of Physalis seedlings occur in PLS.