[Hygiene care and local treatment of skin and mucous membranes in autoimmune bullous diseases]. / Soins d'hygiène et traitements locaux de la peau et des muqueuses dans les maladies bulleuses auto-immunes.

As part of its therapeutic patient education program, the Avicenne hospital in the Paris region invites patients with an autoimmune bullous disease to a workshop dedicated to local skin and mucous membrane care. Together, a nurse, patient partners and patients review best practices in hygiene care and treatment of the skin, eyes, nose, mouth, genitals and anus. This is essential for healing lesions and avoiding local complications.

Cutaneous, Intranasal Blastomycosis Infection in Two Patients from Southern West Virginia: Diagnostic Dilemma.

Blastomycosis is a dimorphic fungus caused by the species of Blastomyces dermatitidisand Blastomyces gilchristii, which are endemic to the Ohio River and Mississippi River Valleys. It is commonly found in soil or decomposing wood. It is capable of infecting both immunocompromised and immunocompetent patients via the respiratory tract by inhaling conidia, where it may remain asymptomatic for a prolonged period of time. Extrapulmonary complications can occur in disseminated disease due to haematogenous spread from the lungs to other organ systems. Haematogenous dissemination from the lungs occurs most commonly to the skin. Although rare, primary cutaneous blastomycosis can also occur with direct inoculation through the trauma of the skin. Patients presenting with cutaneous blastomycosis are often misdiagnosed with malignant neoplasms and may not be appropriately managed until further testing and tissue biopsy. Currently, there are only four previous case reports of blastomycosis presenting in the intranasal region. We report two cases of cutaneous blastomycosis of the nasal passages and upper lip with pulmonary manifestations in Southern West Virginia. These patients first presented with cutaneous symptoms, which were originally treated for melanoma and squamous cell carcinoma and were later diagnosed with systemic blastomycosis.

Uso da cultura de mucosa escamosa humana no tratamento de lesões jugais pré-cancerosas extensas / The use of human squamous mucosa culture in the treatment of extensive buccal premalignant lesions

OBJETIVO: Os autores propõem uma nova abordagem no tratamento de lesões extensas de natureza pré-cancerosa da mucosa jugal, utilizando enxerto de mucosa escamosa autógena cultivada em laboratório. MÉTODO: O enxerto é aplicado no mesmo tempo cirúrgico da ressecção da lesão original. Foram operados cinco pacientes, os quais receberam acompanhamento pós-operatório, sendo submetidos à biopsia de controle no 90º dia. A avaliação da integração do enxerto com o leito receptor foi realizada utilizando-se critérios clínicos e morfológicos, incluindo microscopia óptica e eletrônica. RESULTADOS: O estudo anatomopatológico com microscopia óptica e eletrônica dos cinco pacientes mostrou haver integração do enxerto da mucosa cultivada com o leito receptor. As células da mucosa cultivada formam camadas que se organizam e se diferenciam à semelhança da zona doadora. À microscopia eletrônica a mucosa enxertada apresentava lâmina basal com descontinuidades focais, presença de hemidesmosomas e fibrilas de ancoragem. CONCLUSÕES: Os resultados demonstraram que a técnica é oportuna e viável para o tratamento de lesões pré-cancerosas, outrora consideradas irressecáveis pela extensão e pode ser considerada uma possibilidade real para o tratamento cirúrgico definitivo das mesmas.

OBJECTIVE: The authors propose a new approach to the treatment of extensive pre-malignant lesions of the jugal mucosa, using laboratory grown autogenous squamous mucosa as graft. METHOD: The graft was implanted at the same time the original lesion was excised. Five patients underwent surgery and were followed up until a control biopsy was obtained after 90 days. Both clinical and morphological criteria, including light and electronic microscopy, were used to assess integration of the graft to the receptor bed. RESULTS: The anatomopathological study under light and electronic microscopy of the five patients showed integration of the cultivated mucosa to the receptor bed. Cells from the cultivated mucosa make up layers which organize and differentiate according to the pattern of the donor zone. The ultrastructure of the grafted mucosa showed a basal layer with discrete areas of loss of continuity, hemidesmosomes and anchorage fibrillae. CONCLUSIONS: Our results demonstrated that being a timely and feasible technique for the treatment of pre-malignant lesions, so far deemed not amenable to resection owing to their extension, the technique may be considered a real possibility for the definitive treatment.