Revision of the Original Material of Plagiothecium denticulatum var. obtusifolium (Turn.) Moore and New Synonyms for This Taxon.

Hypnum denticulatum var. obtusifolium was described by Turner in the early nineteenth century. This taxon, now known as Plagiothecium denticulatum var. obtusifolium, has not been studied in detail or received a detailed description. During the revision in the Natural History Museum (herbarium BM), a specimen described there as a type (BM000890810) was found, but a careful analysis showed that it is currently not a type specimen. On the other hand, the conducted research showed that the holotype of this taxon is the figure attached by Turner to the newly described taxon. However, because the holotype does not contain all taxonomically significant features, the specimen found was used to designate the epitype of this name (BM000890810). Therefore, this paper provides the first complete description of the qualitative and quantitative characteristics of this taxon, which is today known as P. denticulatum var. obtusifolium. Forty qualitative and quantitative characteristics analyzed made it possible to make redescription of the examined taxon. Moreover, analysis of two morphologically similar, currently distinct taxa, P. sandbergii and P. denticulatum var. auritum, revealed no differences between them. Therefore, these taxa were proposed as new synonyms of P. denticulatum var. obtusifolium. Additionally, for both taxa mentioned above, lectotypes were proposed for P. denticulatum var. auritum, specimen PC0132639, and for P. sandbergii, specimen PC0132604, both from the Muséum National d'Histoire Naturelle, Paris, France (Herbarium PC).

Taxonomic notes on Sorbusmegalocarpa (Rosaceae) and related taxa.

Four Sorbus taxa endemic to China, S.arguta, S.guanxianensis, S.megalocarpavar.megalocarpa and S.megalocarpavar.cuneata, are morphologically similar to one another in having large brown fruits with persistent calyx and dense lenticels. In literature, either all of the four taxa were accepted, or two of them, S.arguta and S.megalocarpavar.cuneata, were treated as synonyms of S.megalocarpavar.megalocarpa, or S.guanxianensis alone was dubious. In this study, based on morphological comparison, S.arguta is reinstated for its relatively small inflorescence, small fruit and timing of flowering after leaves are unfolded. S.megalocarpavar.cuneata is confirmed as a synonym and S.guanxianensis is proposed as a new heterotypic synonym of S.megalocarpa.

Unveiling an enigma from the Cerrado: taxonomic revision of two sympatric species of Apostolepis Cope, 1862 (Dipsadidae: Xenodontinae: Elapomorphini) from central Brazil

Apostolepis albicollaris and A. cerradoensis are two Elapomorphini snake species, described within a short timespan, from the Cerrado of central Brazil. In their brief descriptions, these two species were diagnosed from congeners largely based on highly variable external morphological characters. Interestingly enough, A. cerradoensis has remained known based on a single specimen since its description. Here, we present a reanalysis of both type specimens, as well as a careful examination of a large series of specimens formerly assigned to these species, based on the comparison of internal and external morphology. We conclude that both species are synonymous, providing evidence for the recognition of A. cerradoensis as a junior synonym of A. albicollaris. Furthermore, an account of its updated diagnosis, morphological variation, geographic distribution, hemipenial morphology, phylogenetic relationships and an osteological description are also provided. We also discuss its conservation status, suggesting that the species is under threat and qualifies to be listed as Vulnerable (VU ab(iii)), considering its rarity, small geographic range, and persistent environmental threats.

A morphological and molecular study of Hydrodynastes gigas (Serpentes, Dipsadidae), a widespread species from South America.

BACKGROUND: Studies with integrative approaches (based on different lines of evidence) are fundamental for understanding the diversity of organisms. Different data sources can improve the understanding of the taxonomy and evolution of snakes. We used this integrative approach to verify the taxonomic status of Hydrodynastes gigas (Duméril, Bibron & Duméril, 1854), given its wide distribution throughout South America, including the validity of the recently described Hydrodynastes melanogigas Franco, Fernandes & Bentim, 2007. METHODS: We performed a phylogenetic analysis of Bayesian Inference with mtDNA 16S and Cytb, and nuDNA Cmos and NT3 concatenated (1,902 bp). In addition, we performed traditional morphometric analyses, meristic, hemipenis morphology and coloration pattern of H. gigas and H. melanogigas. RESULTS: According to molecular and morphological characters, H. gigas is widely distributed throughout South America. We found no evidence to support that H. gigas and H. melanogigas species are distinct lineages, therefore, H. melanogigas is a junior synonym of H. gigas. Thus, the melanic pattern of H. melanogigas is the result of a polymorphism of H. gigas. Melanic populations of H. gigas can be found in the Tocantins-Araguaia basin.

A partial molecular phylogeny of Rhadinaea and related genera (Squamata, Dipsadidae) with comments on the generic assignment of Rhadinaea eduardoi.

The genus Rhadinaea is a diverse clade of New World dipsadid snakes, with 22 species arranged in six recognized species groups. The most recently described species, Rhadinaea eduardoi, was described based on a unique specimen collected in the Santa Catarina Juquila municipality in the Sierra Madre del Sur of southern Oaxaca, Mexico. Here, based on a reexamination of the holotype and the results of a phylogenetic analysis of the holotype of Rhadinaea eduardoi and representatives of several genera closely related to Rhadinaea, we reassessed the generic assignment of Rhadinaea eduardoi. In our phylogenetic hypothesis, R. eduardoi was nested within a strongly supported clade of Coniophanes fissidens samples, thus making Rhadinaea paraphyletic with respect to Coniophanes. Additionally, our reexamination of the holotype of Rhadinaea eduardoi revealed that the alleged presence of a subpreocular scale is only true on the right side of the head, and that this scale appears to be a malformed preocular scale; also, a reduction in dorsal scale rows is present; and posterior enlarged maxillary teeth are grooved. Herein we consider that Rhadinaea eduardoi should be placed in the synonymy of Coniophanes fissidens. Consequently, we recognized only five species groups within the genus Rhadinaea.

Evolutionary relationships among Massospora spp. (Entomophthorales), obligate pathogens of cicadas.

The fungal genus Massospora (Zoopagomycota: Entomophthorales) includes more than a dozen obligate, sexually transmissible pathogenic species that infect cicadas (Hemiptera) worldwide. At least two species are known to produce psychoactive compounds during infection, which has garnered considerable interest for this enigmatic genus. As with many Entomophthorales, the evolutionary relationships and host associations of Massospora spp. are not well understood. The acquisition of M. diceroproctae from Arizona, M. tettigatis from Chile, and M. platypediae from California and Colorado provided an opportunity to conduct molecular phylogenetic analyses and morphological studies to investigate whether these fungi represent a monophyletic group and delimit species boundaries. In a three-locus phylogenetic analysis including the D1-D2 domains of the nuclear 28S rRNA gene (28S), elongation factor 1 alpha-like (EFL), and beta-tubulin (BTUB), Massospora was resolved in a strongly supported monophyletic group containing four well-supported genealogically exclusive lineages, based on two of three methods of phylogenetic inference. There was incongruence among the single-gene trees: two methods of phylogenetic inference recovered trees with either the same topology as the three-gene concatenated tree (EFL) or a basal polytomy (28S, BTUB). Massospora levispora and M. platypediae isolates formed a single lineage in all analyses and are synonymized here as M. levispora. Massospora diceroproctae was sister to M. cicadina in all three single-gene trees and on an extremely long branch relative to the other Massospora, and even the outgroup taxa, which may reflect an accelerated rate of molecular evolution and/or incomplete taxon sampling. The results of the morphological study presented here indicate that spore measurements may not be phylogenetically or diagnostically informative. Despite recent advances in understanding the ecology of Massospora, much about its host range and diversity remains unexplored. The emerging phylogenetic framework can provide a foundation for exploring coevolutionary relationships with cicada hosts and the evolution of behavior-altering compounds.

Historical perspective on the synonymization of the four major pest species belonging to the Bactrocera dorsalis species complex (Diptera, Tephritidae).

An FAO/IAEA-sponsored coordinated research project on integrative taxonomy, involving close to 50 researchers from at least 20 countries, culminated in a significant breakthrough in the recognition that four major pest species, Bactrocera dorsalis, Bactrocera philippinensis, Bactrocera papayae and Bactrocera invadens, belong to the same biological species, Bactrocera dorsalis. The successful conclusion of this initiative is expected to significantly facilitate global agricultural trade, primarily through the lifting of quarantine restrictions that have long affected many countries, especially those in regions such as Asia and Africa that have large potential for fresh fruit and vegetable commodity exports. This work stems from two taxonomic studies: a revision in 1994 that significantly increased the number of described species in the Bactrocera dorsalis species complex; and the description in 2005 of Bactrocera invadens, then newly incursive in Africa. While taxonomically valid species, many biologists considered that these were different names for one biological species. Many disagreements confounded attempts to develop a solution for resolving this taxonomic issue, before the FAO/IAEA project commenced. Crucial to understanding the success of that initiative is an accounting of the historical events and perspectives leading up to the international, multidisciplinary collaborative efforts that successfully achieved the final synonymization. This review highlights the 21 year journey taken to achieve this outcome.

MicroRNA loci support conspecificity of Gyrodactylus salaris and Gyrodactylus thymalli (Platyhelminthes: Monogenea).

The monogenean flatworm Gyrodactylus salaris is a serious threat to wild and farmed Atlantic salmon stocks in Norway. Morphologically, the closely related but harmless Gyrodactylus thymalli on grayling can hardly be distinguished from G. salaris. Until now, molecular approaches could not resolve unambiguously whether G. salaris and G. thymalli represent just one polytypic species, two polytypic species or a complex of more than two species. In the first known genome-wide analysis utilizing 37 conserved microRNA loci, the genetic differentiation of seven populations of G. salaris and G. thymalli was assessed. The concatenated alignment spanned 21,742bp including 62 variable positions. A neighbor-joining cluster analysis did not support any host-based or mitochondrial haplotype-based grouping of strains. We conclude that a two species concept for G. salaris and G. thymalli does not reflect meaningful biological entities. Instead, G. salaris and G. thymalli are just one species comprising several pathogenic and non-pathogenic strains on various primary hosts. Following the International Code for Zoological Nomenclature, G. salaris Malmberg, 1957 is the valid species name with G. thymalli Zitnan, 1960 becoming the junior synonym. Accordingly, the range of G. salaris is significantly increased, given that formerly G. salaris-free countries such as e.g., Great Britain are now within the species' natural range. The synonymization of G. salaris and G. thymalli implies severe challenges to current disease management routines, which assume that G. salaris and G. thymalli are readily distinguishable. Protocols for reliable identification of pathogenic and non-pathogenic strains of G. salaris need to be developed.

The identity of Apostolepis pymi and placement of A. quinquelineata in the synonymy of A. nigrolineata (Serpentes, Xenodontinae) / A identidade de Apostolepis pymi e colocação de A. quinquelineata na sinonímia de A. nigrolineata (Serpentes, Xenodontinae)

The correct application of the names Apostolepis nigrolineata, A. quinquelineata, and A. pymi has been subject of considerable controversy. Based upon review of the literature and the characteristics used to distinguish these species from each other, we conclude that A. pymi is a valid species and that A. quinquelineata is a junior synonym of A. nigrolineata. They differ from each other in hemipenial morphology and coloration. The background color of A. nigrolineata is reddish brown (vs. brown in A. pymi). In addition, only the dorsal region of end of tail is covered by a black band in A. nigrolineata (vs. end of tail completely surrounded by a black band in A. pymi). Both species are sympatrically distributed along the lowlands of Amazonia but A. nigrolineata occurs in both western and eastern regions whereas A. pymi is restricted to eastern regions with additional records from the States of Amazonas, Rondônia, Mato Grosso and an endemic population in the state of Ceará, northeastern Brazil.

A correta aplicação dos nomes Apostolepis nigrolineata, A. quinquelineata, e A. pymi tem sido assunto de considerável controvérsia. Baseado em uma revisão da literatura e das características usadas para distinguir essas espécies umas das outras, nós concluímos que A. pymi é uma espécie válida e que A. quinquelineata é um sinônimo júnior de Apostolepis nigrolineata. Elas diferem uma da outra na morfologia hemipeniana e coloração. A coloração dorsal de A. nigrolineata é marrom avermelhada (vs. marrom em A. pymi). Em adição, apenas a região dorsal do final da cauda de A. nigrolineata é coberta por uma banda preta (vs. o final da cauda é completamente envolto por uma banda preta em A. pymi). Ambas as espécies ocorrem dentro da Amazônia, mas A. nigrolineata ocorre nas regiões oeste e leste enquanto A. pymi é restrita para regiões do leste com registros adicionais para os estados do Amazonas, Mato Grosso, Rondônia e mais uma população endêmica no estado do Ceará, nordeste do Brasil.

New records of paratylenchus micoletzky, 1922 from Brazil with descriptions of two new species (tylenchulidae: nemata).

Six species of Paratylenchus Micoletzky, 1922, two of them new, are reported from the Amazon Basin of Brazil. P. colbrani is reported for the first time in Brazil. Extended ranges, along with additional measurements and descriptions, are given for P. leptus, P. salubris, and P. perlatus. P. mimulus Raski, 1975 is synonymized with P. salubris Raski, 1975. P. flectospiculus n. sp. is distinguished by four lines in the lateral field, short stylet (21-24 mum), and spicules with distinct distal bend ventrad. P. rostrocaudatus n. sp. is distinguished by three lines in the lateral field, short stylet (15-16 mum), and beaked tail terminus.