The evolution and application progress of non-modified drug target discovery CETSA technology / 药学学报

Understanding the research methods for drug protein targets is crucial for the development of new drugs, clinical applications of drugs, drug mechanisms, and the pathogenesis of diseases. Cellular thermal shift assay (CETSA), a target research method without modification, has been widely used since its development. Now, there are various CETSA-based technology combinations, such as mass spectrometry-based cellular thermal shift assay (MS-CETSA), isothermal dose response-cellular thermal shift assay (ITDR-CETSA), amplified luminescent proximity homogeneous assay-cellular thermal shift assay (Alpha-CETSA), etc., which combine their respective advantages and further expand the application scope of CETSA. These technologies are suitable for the entire drug development chain, from drug screening to monitoring the target binding and off-target toxicity of drugs in patients. Based on the author's research experience, this paper reviews the principles of CETSA and related binding technologies, their application in target discovery, and the progress of data processing and analysis in recent years, aiming to provide reference and reference for the further application of CETSA.

An Evaluation of the Safety Effectiveness and Cost of Autonomous Vehicles Based on Multivariable Coupling.

There is a need for in-depth studies of autonomous vehicle safety that evaluate the effectiveness of safety functions and different "atomic" technology combinations for vehicles and roads. In this paper, we provide a crash avoidance effectiveness evaluation model for autonomous vehicles enabled with different sensor combinations based on multiple variables of 14 different "atomic" sensing technologies on the vehicle side and road side, 52 safety functions, and 14 accident types. Meanwhile, a cost-sharing model is developed based on the traveled distance during the life cycle of vehicles and based on the traffic flow over the life cycle of roads to evaluate the unit cost per km of different "atomic" technology combinations. The results clearly show that the cost increases with the addition of "atomic" sensing technologies on the vehicle side, while an increase in crash avoidance effectiveness decreases. It is necessary to switch to V2X and to introduce roadside "atomic" technology combinations to realize better safety effectiveness at a lower cost for vehicles. In addition, a map that covers the safety effectiveness and cost per kilometer of all "atomic" technology combinations is calculated for decision-makers to select combinations under the preconditions of cost and safety.