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Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-906239

RESUMO

Objective:This study aims to investigate the effect of triptonide (TN) on proliferation, cell cycle, apoptosis and expressions of apoptosis-related proteins of human acute monocytic leukemia(AML) cell line SHI-1, and to explore its possible mechanism of action. Method:The thiazolyl blue (MTT) colorimetric assay was applied to detect the inhibitory effect of 20,40,80,160,320 nmol·L<sup>-1</sup> triptonide on the proliferation of SHI-1 cells for 48, 72 h. Changes in SHI-1 cell cycle before and after triptonide treatment were detected by flow cytometry propidium iodide (PI) simple staining, and changes in SHI-1 cell apoptosis before and after triptonide treatment were detected by flow cytometry with AnnexinV/PI double staining. Western blot was applied to detect the protein expression of cysteine protease (Caspase)-3, Caspase-8 and nuclear transcription factor kappaB(NF-<italic>κ</italic>B) in SHI-1 cells before and after treatment with 80, 160 nmol·L<sup>-1 </sup>triptonide. Result:Compared with the blank group, 40,80,160,320 nmol·L<sup>-1</sup> triptonide significantly inhibited the proliferation of SHI-1 cells(<italic>P</italic><0.01) in a dose-dependent manner for 48, 72 h, while 160, 320 nmol·L<sup>-1 </sup> triptonide induced apoptosis of SHI-1 cells(<italic>P</italic><0.01) for 48, 72 h, and 160 nmol·L<sup>-1</sup> triptonide could decrease the S phase ratio of SHI-1 cells(<italic>P</italic><0.01). In addition, compared with the blank group, 80,160 nmol·L<sup>-1</sup> triptonide induced the downregulation of NF-<italic>κ</italic>B significantly(<italic>P</italic><0.01), 160 nmol·L<sup>-1</sup> triptonide induced the downregulation of Caspase-3, Caspase-8 significantly(<italic>P</italic><0.01). Conclusion:Triptonide can inhibit the proliferation and induce apoptosis <italic>in vitro</italic> of SHI-1 cells, which may be related to the reduction of the cells in S phase proportion by triptonide, and the downregulation of the expression levels of Caspase-3, Caspase-8 and NF-<italic>κ</italic>B proteins.

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