Acoustic Assessment of Microstructural Deformation Mechanisms on a Cold Rolled Cu30Zn Brass.

The relationship between acoustic parameters and the microstructure of a Cu30Zn brass plate subjected to plastic deformation was evaluated. The plate, previously annealed at 550 °C for 30 min, was cold rolled to reductions ranging from 10% to 70%. Linear ultrasonic measurements were performed on each of the nine specimens, corresponding to the nine different reductions, using the pulse-echo method to record the times of flight of longitudinal waves along the thickness axis. Subsequently, acoustic measurements were conducted to determine the nonlinear parameter ß through second harmonic generation. Microstructural analysis, carried out by X-ray diffraction, Vickers hardness testing, and optical microscopy, revealed an increase in deformation twins, reaching a maximum at 40% thickness reduction. At higher deformations, the microstructure showed the generation and proliferation of shear bands, coinciding with a decrease in the twinning structure and an increase in dislocation density. The longitudinal wave velocity exhibited a 0.9% decrease at 20% deformation, attributed to dislocations and initial twin formation, followed by a continuous increase up to 2% beyond this point, resulting from the combined effects of twinning and shear banding. The nonlinear parameter ß displayed a notable maximum, approximately one order of magnitude greater than its original value, at 40% deformation. This peak correlates with a roughly tenfold increase in twinning fault probability at the same deformation level.

Equine chorionic gonadotropin (eCG) treatment in heifers: Double ovulation, twinning rate, and pregnancy losses in twin pregnancies.

This study aimed to evaluate the effects of different doses of equine chorionic gonadotropin (eCG; 200 and 300 IU) administered at the end of a fixed-time artificial insemination (FTAI) treatment protocol on ovulation, pregnancy, and twin rates in Bos taurus beef heifers. In addition, pregnancy losses in heifers with singleton and twin pregnancies were determined. A total of 2382 Angus heifers treated with a 6-day estradiol/progesterone-based protocol for FTAI (J-Synch protocol) were randomly allocated to two experimental groups to receive 200 or 300 IU of eCG administered intramuscularly at the time of intravaginal progesterone device removal; FTAI was performed from 60 to 72 h after device removal. The pregnancy rate did not differ (P = 0.89) between the 200 and 300 IU eCG groups. The number of corpus luteum induced by both eCG doses was determined by ultrasonographic examination 14 days after insemination and those treated with 300 IU of eCG had a greater double ovulation rate (P < 0.05). In addition, 300 IU eCG treated heifers had a higher twinning rate on day 30 of gestation (P < 0.05) and parturition (P < 0.05). Pregnancy losses from 30 days of gestation to calving did not differ between heifers treated with 200 and 300 IU of eCG (P = 0.70). However, regardless of the experimental group, heifers bearing twins had greater pregnancy losses than heifers with singletons (P < 0.05). In conclusion, reducing the dose of eCG from 300 to 200 IU under FTAI treatment protocol decreases double ovulation and twinning rates, maintaining a similar pregnancy rate in heifers. Nulliparous cows carrying two fetuses suffer greater pregnancy losses than cows with singletons.

Foreword to the special virtual issue on Modern approaches and tools for teaching crystallography.

A compilation of articles with a strong teaching element published since 2018 is presented alongside an overview of the articles in the special issue on this topic.

Effect of breed and management practices on reproductive and milking performance of rangeland goats.

The traditional goat production systems on arid and semi-arid rangelands of northern Mexico are characterized by minimum external inputs; therefore, little is known about goat response to technological improvements. This observational study aimed to ascertain the effect of using improved goat production technologies and breed on milk yield and reproductive performance of goats on rangeland. The study was conducted from January 2017 to June 2018. Thirty-seven goat herds comprising 6393 animals were used. Goats supplemented with 250 g of concentrate (S-goats; 14% crude protein) 30 days before breeding produced significantly more daily milk yield (218 ± 61 vs. 200 ± 60 g) than the unsupplemented (UNS-goats) goats. Milk production per lactation was 12 percentage points greater in goats with access to plain salt throughout the year than those without salt access. The absence of deworming significantly depressed daily milk yield (189 ± 55 vs. 221 ± 61 for non-treated and treated goats). In 5 months, the lowest total milk yield was for Boer goats (26.9 ± 8.3 kg) and the highest for Nubian goats (36.1 ± 9.2 kg). The kidding rate was significantly higher in the S-goats than in the UNS-goats group (66.9 vs. 62.8%). Salt-supplemented goats had a significantly greater kidding rate than goats not receiving salt (68.5 vs. 61.2%). The lowest kidding rate was for Saanen goats and the highest for Nubian goats (66.2%). These findings indicate that milk yield and reproductive performance were primarily influenced by supplementing grazing goats with concentrates and salt in this semi-arid rangeland.

Síndrome de transfusão feto-fetal: revisão de literatura / Twin-twin transfusion syndrome: a literature review

A síndrome de transfusão feto-fetal é uma complicação das gestações gemelares monocoriônicas. Além de ocorrer comumente no segundo trimestre, apresenta elevada morbimortalidade fetal e neonatal, e taxas de incidência que variam de 10 a 15% dentre todas as gravidezes monocoriônicas. O objetivo deste estudo é realizar uma revisão de literatura a partir de levantamento bibliográfico acerca dos principais aspectos epidemiológicos, clínicos e terapêuticos da STFF. A base de dados PubMed foi consultada, uma vez que os termos de pesquisa utilizados foram "síndrome de transfusão feto-fetal", "diagnóstico" e "tratamento". Obtiveram-se sessenta e oito artigos de revisão de literatura e/ou revisão sistemática, sendo que apenas vinte e nove foram selecionados após aplicação dos critérios de elegibilidade. Em relação à fisiopatologia, a síndrome é explicada pela transferência sanguínea direta entre os fetos gemelares através de anastomoses arteriovenosas placentárias, conceitualmente determinando a existência de um feto receptor e outro doador. Embora as gestantes comumente se apresentam assintomáticas, as repercussões clínicas fetais costumam ser graves. O diagnóstico é exclusivamente ultrassonográfico e deve ser feito o mais precocemente possível, ressaltando-se a importância da detecção da corionicidade da gestação gemelar, além de acompanhamento ultrassonográfico seriado para rastreio do desenvolvimento da síndrome. Apesar de ainda não haver protocolo de tratamento bem estabelecido, a ablação dos vasos placentários a laser é tida como o padrão-ouro dentre as opções terapêuticas disponíveis. Apresenta elevada taxa de sobrevida de pelo menos um dos fetos e baixos índices de sequelas neurológicas neonatais, podendo ser realizada somente até a 26ª semana de gestação.

Twin-twin transfusion syndrome is a complication of monochorionic twin pregnancies. In addition to commonly occurring in the second trimester, it has high fetal and neonatal morbidity and mortality and incidence rates ranging from 10 to 15% among all monochorionic pregnancies. This study aims to perform a literature review based on a bibliographic survey about the main epidemiological, clinical and therapeutic aspects of TTTS. The PubMed database was consulted, as the search terms used were "twin-twin transfusion syndrome", "diagnosis", and "treatment". Sixty-eight literature review and systematic review articles were obtained, and only twenty-nine were selected after applying the eligibility criteria. About the pathophysiology, the syndrome is explained by direct blood transfer between the twin fetuses through placental arteriovenous anastomoses, determining the existence of a recipient fetus and another donor. Although pregnant women are usually asymptomatic, the clinical fetal repercussions are often severe. Diagnosis is exclusively ultrasonographic and must be made as early as possible, emphasizing the importance of detecting chorionicity in twin pregnancy, in addition to serial ultrasonographic follow-up to track the development of the syndrome. Although there is still no well-established treatment protocol, endoscopic laser ablation of vascular anastomoses is considered the gold standard among the available therapeutic options. It has a high survival rate for at least one of the fetuses and low rates of neonatal neurological sequelae and can only be performed until the 26th week of pregnancy.

Atendimento de famílias com crianças pequenas / Care of families with young children / Cuidado de familias con niños pequeños

Este artigo apresenta o atendimento de uma família com gêmeos com três anos e meio de idade. Discute brevemente a importância do atendimento familiar nos casos de suspeita de autismo e no caso de gêmeos, para apresentar a complexidade dos vínculos, da configuração edípica e da própria configuração do ego de cada gêmeo. Traz o relato de uma sessão de família com o referencial da psicanálise dos vínculos que nos dá uma ideia clara das situações importantes que emergem em uma sessão e das intervenções psicanalíticas possíveis neste setting.

This article presents the care of a family with three-and-a-half-year-old twins. Briefly discuss the importance of family care in cases of suspected autism and in the case of twins, to present the complexity of the bonds, the oedipal configuration, and the ego configuration of each twin. It brings the report of a family session with the psychoanalysis of bonds framework that gives us a clear idea of the important situations that emerge in a session and the possible psychoanalytic interventions in thissetting.

Este artículo presenta el cuidado de una familia con gemelos de tres anos y medio de edad. Discute brevemente la importancia del cuidado familiar en casos de sospecha de autismo y en el caso de gemelos, para presentar la complejidad de los lazos, la configuración edípica y la configuración del ego de cada gemelo. Trae el informe de una sesión familiar con el marco de vínculos de psicoanálisis que nos da una idea clara de las situaciones importantes que surgen en una sesión y las posibles intervenciones psicoanalíticas en este entorno.

Monozygotic twinning following embryo biopsy at the blastocyst stage.

OBJECTIVE: Monozygotic twinning incidence following preimplantation genetic testing in embryos at cleavage-stage does not appear to increase; however, data regarding the possible impact of the blastocyst-stage preimplantation genetic testing is lacking. We compared the incidence of monozygotic twinning in preimplantation genetic testing cycles performed at the blastocyst-stage, versus cycles without PGT, following single embryo transfer. METHODS: In this retrospective cohort study, we analyzed the incidence of twin pregnancies in patients undergoing intracytoplasmic sperm injection and blastocyst-preimplantation genetic testing (253 cycles), versus a period-matched control population of patients undergoing intracytoplasmic sperm injection and single embryo transfer without preimplantation genetic testing (606 cycles). RESULTS: The overall monozygotic twinning rate was 14/859 (1.6%) per clinical pregnancy. The incidence of zygotic splitting following intracytoplasmic sperm injection and preimplantation genetic testing was 3.5% (95% Confidence interval 1.8%-6.6%) versus 0.8% (95% Confidence interval 0.3%-1.9%) following intracytoplasmic sperm injection without preimplantation sperm injection. After adjusting for potential confounders, preimplantation genetic testing cycles were associated with an increase in the incidence of monozygotic twinning when compared to cycles without embryo biopsy (Odd ratio 3.44, 95% Confidence interval 1.05-11.27, p=0.041). CONCLUSIONS: Our findings indicate that embryo biopsy for preimplantation genetic testing performed at the blastocyst stage is associated to an increase in the incidence of monozygotic twinning. Further validation in larger sample size studies is warranted. Patients undergoing preimplantation genetic testing must receive proper counselling about the potential risks of the technique.

Dental anomalies in the deciduous dentition of non-syndromic oral clefts patients / Anomalias dentárias na dentição decídua de pacientes com fissuras orais não sindrômicas

Abstract Objectives: to investigate the prevalence of dental anomalies in complete deciduous dentition of children with NSCL/P. Methods: this study included 75 children with NSCL/P and 286 healthy control. In both groups the children had deciduous dentition with ages varying from 4 to 6 years. Clinical examination, panoramic and periapical radiographies were performed and dental anomalies of number and shape were considered. Results: there was a higher prevalence of dental anomalies in the case group, compared to the control group. In all, 42 dental anomalies were identified, 25.33% in the case group and 8.04% in control group (p<0.001). Therewas a higher frequency of dental anomalies in NSCL/P (47.36%), followed by non-syndromic cleft lip (31.57%) and non-syndromic cleft palate (21.05%). The occurrence of agenesis (p= 0.005) and twinning (p = 0.029) were higher in the case group. Conclusions: the occurrence of agenesis and dental twinning was more frequent in the case group and may contribute to the definition of oral cleft subphenotype.

Resumo Objetivos: investigar a prevalência de anomalias dentárias na dentição decídua completa de crianças com FL/PNS. Métodos: este estudo incluiu 75 crianças com FL/PNS e 286 controles saudáveis. Em ambos os grupos as crianças tinham dentição decídua com idade variando de 4 a 6 anos. Exame clínico, radiografias panorâmicas e periapicais foram realizadas e anomalias dentárias de número e forma foram consideradas. Resultados: houve maior prevalência de anomalias dentárias no grupo caso, comparado ao grupo controle. Ao todo 42 anomalias dentárias foram identificadas, 25,33% no grupo caso e 8,04% no grupo controle (p<0,001). Houve maior frequência de anomalias dentárias na FL/PNS (47,36%), seguida da fissura labial não sindrômica (31,57%) e da fissura palatina não sindrômica (21,05%). A ocorrência de agenesia (p= 0,005) e geminação (p=0,029) foram maiores no grupo caso. Conclusão: a ocorrência de agenesia e geminação dentária foram mais frequentes no grupo caso e pode contribuir para a definição de subfenótipos de fissuras orais.

Genetic variants linked to folliculogenesis and successful pregnancy are not associated with twin births in a twins' town.

Background: Cândido Godói (CG) is a small city in South Brazil in which natural twin births (both monozygotic and dizygotic) occur at an unusually high rate and the twin trait runs through the local families, which are mostly European descendants. We have argued that a genetic founder effect must have occurred during the settlement of CG and that genetic factors may help to explain the familial aggregation of twinning in that city.Objective: The aim of this study was to evaluate single nucleotide polymorphisms (SNPs) related to folliculogenesis (rs6166:C > T in FSHR, rs11031006:G > A near FSHB, and rs17293443:T > C in SMAD3) and successful pregnancies (rs2010963:C > G in VEGFA, rs1800629:G > A in TNF, rs1801131:T > G and rs1801133:G > A in MTHFR) in mothers from CG.Study design: Forty-four mothers of twins (the case group) and 102 mothers of singletons (the control group) from CG were investigated. Genotypes were determined using real-time PCR (TaqMan® SNP Genotyping Assay).Results: For all SNPs, the distributions of the genotypic and allelic frequencies were similar between cases and controls. Interestingly, a deviation from the Hardy-Weinberg equilibrium was detected for SNP rs11031006:G > A near FSHB in the control population. Different combinations of risk alleles and haplotypic analyses were homogeneously distributed between cases and controls.Conclusion: These results suggest a lack of association between the seven studied SNPs and twin births in CG. However, we hypothesized that other genetic variants related to folliculogenesis or successful pregnancies may be involved in this phenomenon. Identifying such genetic components may be important not only for the Brazilian "Twins' Town" but also for a better understanding of twinning in general.

Twinning Rates in Uruguay Between 1999 and 2015: Association with Socioeconomic and Demographic Factors.

Twinning is rare among humans, but there is much variability among populations. Several studies show that certain demographic and socioeconomic factors, such as maternal age, mother's educational level and income, influence twinning rate. There is no background of analytical studies of twins in Uruguay. To the best of our knowledge, this is the first study that has focused on describing and analyzing Uruguayan twinning rates over a period of 17 years (1999-2015). The birth data were collected from the website of Uruguay's Ministry of Public Health. Economic data were obtained from Uruguay's Instituto Nacional de Estadísti's website for the period 2001-2013, since these variables are defined specifically for that period of time. The statistical software R (The R Project for Statistical Computing) was used. The twinning rate varied from 8.51 to 13 in the studied period. Montevideo has the highest median and the smallest variability in comparison with the other departments. In Uruguay (1999-2015), the highest twinning rate (28.94%) was observed in women aged 45 and older. The analysis also showed a relationship between twin birth rates and the mother's educational level. In three regions of the country (West, Center and East), twin births show a random pattern but in the other two (North and Metropolitan), there is an increasing trend in the number of twins over time. In conclusion, this study recognizes social, economic and demographic factors that influence in the rate of twin births in Uruguay.

Classics revisited: Miguel Fernández on germ layer inversion and specific polyembryony in armadillos.

BACKGROUND: Miguel Fernández was an Argentinian zoologist who published the first account of obligate polyembryony in armadillos. His contribution is here discussed in relation to his contemporaries, Newman and Patterson, and more recent work. FINDINGS: Fernandez worked on the mulita (Dasypus hybridus). He was able to get early stages before twinning occurred and show it was preceded by inversion of the germ layers. By the primitive streak stage there were separate embryonic shields and partition of the amnion. There was, however, a single exocoelom and all embryos were enclosed in a common set of membranes comprising chorion towards the attachment site in the uterine fundus and inverted yolk sac on the opposite face. He showed that monozygotic twinning did not occur in another armadillo, the peludo (Chaetophractus villosus). CONCLUSIONS: Fernández's work represented a major breakthrough in understanding how twinning occurred in armadillos. His work and that of others is of intrinsic interest to zoologists and has a direct bearing on the origin of monozygotic twins and birth defects in humans.

Secuencia de perfusion arterial reversa (TRAP) / Twin Reversed Arterial Perfusion, TRAP

Resumen:Considerada como una complicación exclusiva de gestaciones múltiples monocoriales, la secuencia de perfusión arterial reversa se caracteriza por la presencia de un feto malformado que tiene un corazón rudimentario o ausente, con una perfusión desde el gemelo estructuralmente normal hacia el feto acárdico y una mortalidad del 100% en el feto acárdico y el 50% en el feto sano. Se reporta un caso clínico de una paciente de 15 años con embarazo gemelar monocorial de 27 semanas, referida al Hospital San Juan de Dios, en donde se le dio seguimiento diario con ultrasonido y se documentó el patrón ultrasonógrafico anormal de sumación-colisión. A los 7 días de ingreso se decide la interrupción del embarazo por sospecha de coriamnioitis, naciendo un producto viable y otro polimalformado.

Abstract:Considered an exclusive complication of monochorionic gestations, the TRAP sequence is characterize by the presence of a malformed fetus with a rudimentary or absent heart, in which there is an perfusion from the structurally normal twin to the acardiac fetus and a 100% mortality in the acardiac fetus and 50% in the healthy one. We present a clinical case of a 15 years old patient with monochorionic twin pregnancy of 27 weeks, referred to Hospital San Juan de Dios, where daily monitoring with ultrasound was done and an abnormal collision-summantion pattern was documented and reported. After 7 days of admission chorioamnionitis was suspected and termination of the pregnancy was decided resulting in a viable product and another with multiple malformations.

A case of monocephalusrachipagus tribrachius tetrapus in a puppy.

Caudal duplication (dipygus) is an uncommon pathologic of conjoined twinning. The conjoined malformation is classified according to the nature and site of the union. We report the presence of this malformation in a female crossbreed puppy. The puppy was delivered by caesarean section following a prolonged period of dystocia. External findings showed a single head (monocephalus) and a normal cranium with no fissure in the medial line detected. The thorax displayed a caudal duplication arising from the lumbosacral region (rachipagus). The puppy had three upper limbs, a right and left, and a third limb in the dorsal region where the bifurcation began. The subsequent caudal duplication appeared symmetrical. Necropsy revealed internal abnormalities consisting of a complete duplication of the urogenital system and a duplication of the large intestines arising from a bifurcation of the caudal ileum . Considering the morphophysiological description the malformation described would be classified as the first case in the dog of a monocephalusrachipagustribrachius tetrapus.

Twinning and Multiple Birth Rates According to Maternal Age in the City of São Paulo, Brazil: 2003-2014.

The present study investigates the twinning rates in the city of São Paulo, Brazil, during the years 2003-2014. The data were drawn from the Brazilian Health Department database of Sistema de Informações de Nascidos Vivos de São Paulo-SINASC (Live Births Information System of São Paulo). In general, more information is available on the incidence of twinning in developed countries than in developing ones. A total of 24,589 twin deliveries and 736 multiple deliveries were registered in 140 hospitals of São Paulo out of a total of 2,056,016 deliveries during the studied time period. The overall average rates of singleton, twin, and multiple births per 1,000 maternities (‰) were 987.43, 11.96 (dizygotic (DZ) rate was 7.15 and monozygotic (MZ) 4.42), and 0.36, respectively. We further regressed maternal age and historical time period on percentage of singleton, twin, and multiple birth rates. Our results indicated that maternal age strongly positively predicted twin and multiple birth rates, and negatively predicted singleton birth rates. The historical time period also positively, although weakly, predicted twin birth rates, and had no effect on singleton or multiple birth rates. Further, after applying Weinberg's differential method, we computed regressions separately for the estimated frequencies of DZ and MZ twin rates. DZ twinning was strongly positively predicted by maternal age and, to a smaller degree, by time period, while MZ twinning increased marginally only with higher maternal age. Factors such as increasing body mass index or air pollution can lead to the slight historical increase in DZ twinning rates. Importantly, consistent with previous cross-cultural and historical research, our results support the existence of an age-dependent physiological mechanism that leads to a strong increase in twinning and multiple births, but not singleton births, among mothers of higher age categories. From the ultimate perspective, twinning and multiple births in later age can lead to higher individual reproductive success near the end of the reproductive career of the mother.

A polymeric silver thiosaccarinate complex with a two-dimensional triply entangled mesh and argentophilic interactions.

Silver(I) complexes with sulfur-donor ligands have a broad range of pharmacological applications. One of the most important factors for tuning the biological activity is the type of donor atom and the ease of ligand replacement. Silver thiosaccharinates display a wide range of structures from mono- to polynuclear complexes. We report the synthesis, crystal structure and vibrational spectroscopic analysis of a two-dimensional Ag(I)-thiosaccharinate coordination polymer, namely poly[tris(µ2-4,4'-bipyridine-κ(2)N:N')bis(µ3-1,1-dioxo-1,2-benzisothiazole-3-thiolato-κ(3)N:S(3):S(3))bis(µ2-1,1-dioxo-1,2-benzisothiazole-3-thiolato-κ(2)S(3):S(3))tetrasilver(I)], [Ag2(C7H4NO2S2)2(C10H8N2)1.5]n, with 4,4'-bipyridine acting as a spacer. A relevant feature of the structure is the presence of an unusually short Ag...Ag separation of 2.8859 (10) Å, well within the range of argentophilic interactions and confirmed as such by Raman analysis of the low-frequency spectrum. From a topological point of view, the structure presents interpenetration in the form of a threefold entangled 2D→2D mesh (2D is two-dimensional).

Climatic conditions, twining and frequency of milking as factors affecting the risk of fetal losses in high-yielding Holstein cows in a hot environment.

An epidemiological study of risk factors for fetal losses was carried out on 62,403 high-yielding Holstein cows in 29 large highly technified dairy herds in northern Mexico (25° N; 23.5 °C mean annual temperature). Multivariate multiple-group response model indicated that fetal losses between 43 and 260 days of pregnancy were 23 %. Heat-stressed cows at conception (temperature-humidity index, THI >82) were 14 times more likely (P < 0.01) to present fetal losses than not heat-stressed cows (27 vs. 18 %). Heat-stressed cows at 60 days of pregnancy (THI >82) were 4.5 times more likely (P < 0.01) to present fetal losses than cows suffering heat stress in early gestation (29.1 vs. 17.7 %). The proportion of cows experiencing fetal loss was lower for multiparous than primiparous cows (odds ratio; OR = 0.7). Cows with twin pregnancies had significantly increased chances of losing their fetuses than cows with a single fetus (33.6 vs. 20.7 %; P < 0.01). Cows with three milkings per day were 30 % more likely (P < 0.01) to lose their fetuses than cows milked twice daily. Cows calving in winter and spring had significantly increased chances of losing their fetuses than cows calving in summer and fall (30-35 vs. 4-5 %; P < 0.01). It was concluded that, in this particular environment, heat stress exert a great influence on fetal losses in high producing Holstein cows.

Possible asynchronous parturition in a multifetal strepsirrhine: Hapalemur meridionalis

Although infrequent among primates, twinning is common among strepsirrhines, a group of primates that includes the lemurs of Madagascar. As with any multifetal pregnancy, complications during gestation or even parturition can arise due to various factors (e.g., amniotic infection, stress). On rare occasions during labour of twins, parturition may be delayed between the two neonates, a phenomenon known as ‘delayed interval delivery’ that has been well-documented among the human medical journals. Based on circumstantial evidence, we report an opportunistic account of a habituated, adult female southern bamboo lemur (Hapalemur meridionalis) from Mandena, southeast Madagascar, first giving birth to an infant in midOctober 2013 (found deceased), and then birthing a live infant sometime between 5-21 days later. Similar to cases reported in human medical literature, the second infant survived and appeared to stay healthy until the conclusion of the study. Our account of asynchronous parturition of twins in a non-human primate is, to our knowledge, the first reported case in the literature.

Possible asynchronous parturition in a multifetal strepsirrhine: Hapalemur meridionalis

Although infrequent among primates, twinning is common among strepsirrhines, a group of primates that includes the lemurs of Madagascar. As with any multifetal pregnancy, complications during gestation or even parturition can arise due to various factors (e.g., amniotic infection, stress). On rare occasions during labour of twins, parturition may be delayed between the two neonates, a phenomenon known as ‘delayed interval delivery that has been well-documented among the human medical journals. Based on circumstantial evidence, we report an opportunistic account of a habituated, adult female southern bamboo lemur (Hapalemur meridionalis) from Mandena, southeast Madagascar, first giving birth to an infant in midOctober 2013 (found deceased), and then birthing a live infant sometime between 5-21 days later. Similar to cases reported in human medical literature, the second infant survived and appeared to stay healthy until the conclusion of the study. Our account of asynchronous parturition of twins in a non-human primate is, to our knowledge, the first reported case in the literature.(AU)

Clinical and cytogenetic features of a Brazilian sample of patients with phenotype of oculo-auriculo-vertebral spectrum: a cross-sectional study / Características clínicas e citogenéticas de uma amostra brasileira de pacientes com fenótipo de espectro óculo-aurículo-vertebral: um estudo transversal

CONTEXT AND OBJECTIVE: Oculo-auriculo-vertebral spectrum (OAVS) is considered to be a defect of embryogenesis involving structures originating from the first branchial arches. Our objective was to describe the clinical and cytogenetic findings from a sample of patients with the phenotype of OAVS. DESIGN AND SETTING: Cross-sectional study in a referral hospital in southern Brazil. METHODS: The sample consisted of 23 patients who presented clinical findings in at least two of these four areas: orocraniofacial, ocular, auricular and vertebral. The patients underwent a clinical protocol and cytogenetic evaluation through high-resolution karyotyping, fluorescence in situ hybridization for 5p and 22q11 microdeletions and investigation of chromosomal instability for Fanconi anemia. RESULTS: Cytogenetic abnormalities were observed in three cases (13%) and consisted of: 47,XX,+mar; mos 47,XX,+mar/46,XX; and 46,XX,t(6;10)(q13; q24). We observed cases of OAVS with histories of gestational exposition to fluoxetine, retinoic acid and crack. One of our patients was a discordant monozygotic twin who had shown asymmetrical growth restriction during pregnancy. Our patients with OAVS were characterized by a broad clinical spectrum and some presented atypical findings such as lower-limb reduction defect and a tumor in the right arm, suggestive of hemangioma/lymphangioma. CONCLUSIONS: We found a wide range of clinical characteristics among the patients with OAVS. Different chromosomal abnormalities and gestational expositions were also observed. Thus, our findings highlight the heterogeneity of the etiology of OAVS and the importance of these factors in the clinical and cytogenetic evaluation of these patients. .

CONTEXTO E OBJETIVO: O espectro oculoauriculovertebral (EOAV) é considerado um defeito de embriogênese envolvendo estruturas originadas a partir dos primeiros arcos branquiais. Nosso objetivo foi descrever os achados clínicos e citogenéticos de uma amostra de pacientes com fenótipo de EOAV. TIPO DE ESTUDO E LOCAL: Estudo transversal em um hospital de referência no sul do Brasil. MÉTODOS: A amostra foi composta de 23 pacientes que apresentaram achados clínicos em pelo menos duas das quatro áreas: orocraniofacial, oculares, auriculares e vertebrais. Os pacientes foram submetidos a um protocolo clínico e avaliação citogenética através do cariótipo de alta resolução, hibridização in situ fluorescente para as microdeleções 5p e 22q11 e pesquisa de instabilidade cromossômica para anemia de Fanconi. RESULTADOS: Alterações citogenéticas foram observadas em três casos (13%) e consistiam de: 47,XX,+mar; mos 47,XX,+mar/46,XX e 46,XX,t(6;10)(q13;q24). Observamos casos de EOAV com história de exposição gestacional à fluoxetina, ácido retinoico e crack. Um dos nossos pacientes foi um gêmeo monozigótico discordante que teve restrição de crescimento assimétrica durante a gravidez. Nossos pacientes com EOAV foram caracterizados por um amplo espectro clínico e alguns apresentaram achados clínicos atípicos como um defeito de redução de membro inferior e um tumor do braço direito, sugestivo de hemangioma/linfangioma. CONCLUSÕES: Verificamos grande variedade de características clínicas entre os pacientes com EOAV. Também foram observadas diferentes anomalias cromossômicas e exposições gestacionais. Assim, nossos achados salientam a heterogeneidade da etiologia do EOAV e a importância desses fatores na avaliação clínica e citogenética desses pacientes. .

Os sintomas psicofuncionais e a relação mãe-bebês gêmeos aos nove meses de idade / Symptoms psychofunctional and the mother-twin babies at nine months of age / Los síntomas psicofuncionales y los bebés gemelos de madre a los nueve meses de edad

Os sintomas psicofuncionais manifestados pelo bebê se refletem no comportamento disfuncional da criança e se manifestam nas áreas relacionadas ao sono, alimentação, digestão, respiração, pele e comportamento. A literatura aponta para a existência de uma relação entre a presença de tais sintomas no bebê e a dinâmica da relação deste com a mãe. A partir de uma perspectiva psicanalítica, a presente pesquisa, de caráter exploratório e abordagem qualitativa, utilizou o delineamento de estudo de caso único para investigar a relação mãe-bebês, em situação de gemelaridade, cujos bebês apresentam indicadores de sintomas psicofuncionais aos nove meses de idade. Participaram deste estudo uma tríade formada pela mãe e seus bebês gêmeos com idade de nove meses. Os instrumentos utilizados foram o Symptom Check-List, uma ficha de dados sócio-demográficos, entrevista semi-estruturada com a mãe e sessão de observação da interação livre da mãe com os bebês. Os resultados apontam que a gemelaridade pode configurar-se como um dos fatores que pode aumentar as chances do surgimento dos sintomas psicofuncionais em fase precoce do desenvolvimento. Além disso, a conflitiva conjugal aliada à falta de apoio paterno, em especial em situação de gemelaridade, pode potencializar as chances de surgimento de falha materna e consequente risco ao desenvolvimento de sintomas psicofuncionais nos bebês. Desta forma, ampliar a compreensão acerca do funcionamento de uma tríade pode contribuir como medida preventiva e fornecer subsídios para profissionais de diversas áreas de atuação que trabalham com bebês e suas mães como escolas, hospitais, clínicas e psicoterapeutas da relação pais-bebê.

The symptoms manifested by psychofunctional baby are reflected in the child's dysfunctional behavior and manifested in the areas related to sleep, feeding, digestion, respiration, skin and behavior. The literature points to the existence of a relationship between the presence of such symptoms in the baby and the dynamics of its relationship with the mother. From a psychoanalytic perspective, this research, exploratory and qualitative approach, we used the design of a single case study to investigate the mother-infant in situation of twins, whose babies have symptoms indicators psychofunctional nine months age. The study included a triad formed by the mother and her twin babies aged nine months. The instruments used were the Symptom Check List, a form of socio-demographic data, semi-structured interview with the mother and observing session of free interaction of mothers with babies. Results show that the twins can set up as one of the factors that can increase the chances of the onset of symptoms psychofunctional in early stage of development. Moreover, the conflictive marital coupled with lack of parental support, especially in situations of multiple births, can enhance the chances of emergence of maternal failure and consequent risk to develop symptoms in babies psychofunctional. Thus, increasing the understanding of the functioning of a triad can contribute as a preventive measure and provide subsidies for professionals from various fields of expertise who work with babies and their mothers as schools, hospitals, clinics and therapists of parent-infant relationship.

Los síntomas se manifiestan por psicofuncionales bebé se reflejan en el comportamiento disfuncional del niño y que se manifiesta en los ámbitos relacionados con el sueño, la alimentación, la digestión, la respiración, la piel y el comportamiento. La literatura apunta a la existencia de una relación entre la presencia de tales síntomas en el bebé y la dinámica de su relación con la madre. Desde una perspectiva psicoanalítica, la investigación, el enfoque exploratorio y cualitativo, se utilizó el diseño de un estudio de caso único para investigar la relación madre-niño en situación de gemelos, cuyos bebés tienen indicadores de síntomas psicofuncionales nueve meses edad. El estudio incluyó una tríada formada por la madre y sus bebés gemelos de nueve meses. Los instrumentos utilizados fueron la Lista de verificación de síntomas, una forma de datos sociodemográficos, semi-estructurada entrevista con la madre y la sesión de observación de la libre interacción de las madres con bebés. Los resultados muestran que los gemelos pueden establecerse como uno de los factores que pueden aumentar las probabilidades de la aparición de los síntomas psicofuncionales en la etapa inicial de desarrollo. Por otra parte, el acoplado conflictivo matrimonio con la falta de apoyo de los padres, especialmente en situaciones de nacimientos múltiples, puede aumentar las posibilidades de aparición de insuficiencia materna y el consiguiente riesgo de desarrollar síntomas en los bebés psicofuncionales. Por lo tanto, el aumento de la comprensión del funcionamiento de una tríada puede contribuir como una medida preventiva y proporcionar subvenciones para profesionales de diversas áreas de conocimiento que trabajan con bebés y sus madres como escuelas, hospitales, clínicas y terapeutas de la relación padre-hijo.