RESUMO
Micellar electrokinetic chromatography was used to study the behavior of quinine, propranolol, bilirubin, biliverdin dimethyl ester, and xanthobilirubin methyl ester in single and binary bile salt micelle systems comprised of glycocholic acid and glycodeoxycholic acid. Micelle systems studied had total bile salt concentrations in the range of 10-33 mM with molar ratios of 1:0, 2:1, 1:1, 1:2, and 0:1 glycocholic acid:glycodeoxycholic acid. A pH 8.5 phosphate-borate buffer system was used. For all analytes except bilirubin, the smallest migration factors were found in glycocholic acid solutions and the largest in glycodeoxycholic acid solutions. Intermediate migration factors were found for all compounds except bilirubin in the binary bile salt systems. Bilirubin behaved uniquely with its largest migration factors in the binary bile salt mixtures.
Assuntos
Bilirrubina/isolamento & purificação , Cromatografia Capilar Eletrocinética Micelar/métodos , Ácidos e Sais Biliares , Ácido Glicocólico/isolamento & purificação , Ácido Glicodesoxicólico/isolamento & purificação , MicelasRESUMO
Separation of the glycine and taurine conjugates of ursodeoxycholic acid from those of lithocholic acid, chenodeoxycholic acid, deoxycholic acid, and cholic acid by thin-layer chromatography is described. Thus, on running a silica gel G plate first in a solvent system of n-butanol-water 20:3 and then in a second solvent system of chloroform-isopropanol-acetic acid-water 30:20:4:1, all the above-mentioned conjugated bile acids are separated from one another. The application of this method to study the change in the biliary bile acid conjugation pattern in ursodeoxycholic acid-fed gallstone patients is described.
