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1.
Biosens Bioelectron ; 155: 112094, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-32090867

RESUMO

A robust amperometric sensor was developed for the lactate detection in the extracellular matrix of cancer cells. The sensor was fabricated by separately immobilizing nicotinamide adenine dinucleotide (NAD+) onto a carboxylic acid group and lactate dehydrogenase (LDH) onto an amine group of bi-functionalized conducting polymer (poly 3-(((2,2':5',2″-terthiophen)-3'-yl)-5-aminobenzoic acid (pTTABA)) composited with N, S-doped porous carbon. Morphological features of the composite layer and sensor performance were investigated using FE-SEM, XPS, and electrochemical methods. The experimental parameters were optimized to get the best results. The calibration plot showed a linear dynamic range between 0.5 µM and 4.0 mM with the detection limit of 112 ± 0.02 nM. The proposed sensor was applied to detect lactate in a non-cancerous (Vero) and two cancer (MCF-7 and HeLa) cell lines. Among these cell lines, MCF-7 was mostly affected by the administration of lactate transport inhibitor, α-cyano-4-hydroxycinnamate (αCHC), followed by HeLa and Vero, respectively. Furthermore, the effect of αCHC concentration and treatment time on the lactate level in the cell lines were demonstrated. Finally, cytotoxicity studies were also performed to evaluate the effect of αCHC on cell viability.


Assuntos
Técnicas Biossensoriais/métodos , Ácido Láctico/análise , Nanotecnologia/métodos , Polímeros , Animais , Técnicas Biossensoriais/normas , Carbono , Linhagem Celular Tumoral , Ácidos Cumáricos/antagonistas & inibidores , Técnicas Eletroquímicas , Enzimas Imobilizadas , Humanos , L-Lactato Desidrogenase , Sondas Moleculares , Nanotecnologia/normas , Reprodutibilidade dos Testes
2.
Eur J Pharmacol ; 679(1-3): 68-74, 2012 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-22266492

RESUMO

Ferulic acid (4-hydroxy-3-methoxycinnamic acid) is a phenolic compound present in several plants with claimed beneficial effects in prevention and treatment of disorders linked to oxidative stress and inflammation. In this study, we aimed to verify the possible antidepressant-like effect of acute oral administration of ferulic acid in the forced swimming test (FST) and tail suspension test (TST) in mice. Additionally, the mechanisms involved in the antidepressant-like action and the effects of the association of ferulic acid with the antidepressants fluoxetine, paroxetine, and sertraline in the TST were investigated. Ferulic acid produced an antidepressant-like effect in the FST and TST (0.01-10 mg/kg, p.o.), without accompanying changes in ambulation. The pretreatment of mice with WAY100635 (0.1 mg/kg, s.c., a selective 5-HT(1A) receptor antagonist) or ketanserin (5 mg/kg, i.p., a 5-HT(2A) receptor antagonist) was able to reverse the anti-immobility effect of ferulic acid (0.01 mg/kg, p.o.) in the TST. The combination of fluoxetine (5 mg/kg, p.o.), paroxetine (0.1 mg/kg, p.o.) or sertraline (1 mg/kg, p.o.) with a sub-effective dose of ferulic acid (0.001 mg/kg, p.o.) produced a synergistic antidepressant-like effect in the TST, without causing hyperlocomotion in the open-field test. Taken together, these results demonstrate that ferulic acid exerts antidepressant-like effect in the FST and TST in mice through modulation of the serotonergic system.


Assuntos
Antidepressivos/farmacologia , Ácidos Cumáricos/farmacologia , Inibidores Seletivos de Recaptação de Serotonina/farmacologia , Antagonistas do Receptor 5-HT1 de Serotonina/farmacologia , Antagonistas do Receptor 5-HT2 de Serotonina/farmacologia , Animais , Ácidos Cumáricos/agonistas , Ácidos Cumáricos/antagonistas & inibidores , Relação Dose-Resposta a Droga , Interações Medicamentosas , Fluoxetina/farmacologia , Resposta de Imobilidade Tônica/efeitos dos fármacos , Ketanserina/farmacologia , Masculino , Camundongos , Atividade Motora/efeitos dos fármacos , Paroxetina/farmacologia , Piperazinas/farmacologia , Piridinas/farmacologia , Receptor 5-HT1A de Serotonina , Receptor 5-HT2A de Serotonina , Sertralina/farmacologia
3.
Pharmazie ; 63(4): 290-5, 2008 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-18468389

RESUMO

Rhodiola has been widely used in traditional Asian medicine. In this study, we tested the hypopigmentation effects of R. sachalinensis and its active compounds including catechin, chlorogenic acid, p-coumaric acid, and p-tyrosol. Results have shown that only p-coumaric acid inhibits melanin synthesis in B16F10 cells. However, p-coumaric acid did not inhibit tyrosinase activity when L-DOPA was used as a substrate. Instead, p-coumaric acid inhibited tyrosinase activity when L-tyrosine was used as a substrate. We further analyzed the changes of cAMP responsive element binding protein (CREB) phosphorylation and tyrosinase gene expression. The results indicate that p-coumaric acid does not affect CREB phosphorylation or tyrosinase protein production. In turn, these findings demonstrate that p-coumaric acid has no effect on the upstream regulation of tyrosinase gene expression, although p-coumaric acid showed a significant inhibitory effect on melanogenesis. Because p-coumaric acid showed different effects on tyrosinase activity according to different substrates, we tested whether tyrosinase can utilize p-coumaric acid as a substrate. Our findings revealed that competitive inhibition occurs between p-coumaric acid and tyrosine. Consequently, this finding could be a primary mechanism for the hypopigmenting action of p-coumaric acid.


Assuntos
Ácidos Cumáricos/farmacologia , Melaninas/antagonistas & inibidores , Melaninas/biossíntese , Rhodiola/química , Western Blotting , Catequina/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Ácido Clorogênico/farmacologia , Ácidos Cumáricos/antagonistas & inibidores , Ácidos Cumáricos/química , Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico/metabolismo , Humanos , Levodopa/metabolismo , Melanoma Experimental/metabolismo , Monofenol Mono-Oxigenase/antagonistas & inibidores , Álcool Feniletílico/análogos & derivados , Álcool Feniletílico/farmacologia , Fosforilação , Propionatos , Tirosina/antagonistas & inibidores , Tirosina/farmacologia , alfa-MSH/farmacologia
4.
Hypertens Res ; 25(1): 99-107, 2002 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-11924733

RESUMO

The effects of a water-soluble green coffee bean extract (GCE) on blood pressure were investigated using spontaneously hypertensive rats (SHR). There was a dose-dependent reduction in blood pressure after a single ingestion (180 to 720 mg/kg, p.o.) or long-term ingestion (0.25 to 1% diet for 6 weeks) of GCE. A single oral ingestion (50 to 200 mg/kg) of 5-caffeoylquinic acid (5-CQA), the major component of GCE, dose-dependently decreased blood pressure, suggesting that 5-CQA is involved in the hypotensive effect of GCE in SHR. Because significant increases in caffeic acid (CA) or ferulic acid (FA) were detected in plasma after oral ingestion of 5-CQA in SHR, these acids (2.5, 5,10 micromol/kg) were intravenously injected into SHR under anesthesia and the carotid arterial pressure was measured. Of the two components, FA had a stronger depressor effect than CA. The depressor effect of FA (50 mg/kg, p.o.) was attenuated by the concurrent injection of atropine sulfate (5 mg/kg, s.c.), suggesting that the hypotensive effect of FA in SHR might be mediated via the muscarinic acetylcholine receptors. These findings indicate that oral ingestion of GCE or 5-CQA decreases blood pressure in SHR, and that FA, which is a metabolite of 5-CQA, is a candidate hypotensive component.


Assuntos
Anti-Hipertensivos/farmacologia , Ácidos Cafeicos/farmacologia , Café/química , Ácidos Cumáricos/farmacologia , Hipertensão/fisiopatologia , Extratos Vegetais/farmacologia , Ácido Quínico/análogos & derivados , Ácido Quínico/farmacologia , Ratos Endogâmicos SHR/fisiologia , Administração Oral , Animais , Atropina/farmacologia , Pressão Sanguínea/efeitos dos fármacos , Artérias Carótidas/efeitos dos fármacos , Artérias Carótidas/fisiologia , Ácidos Cumáricos/antagonistas & inibidores , Injeções Intravenosas , Injeções Subcutâneas , Masculino , Antagonistas Muscarínicos/farmacologia , Ratos , Ratos Endogâmicos WKY , Fatores de Tempo
5.
Biochim Biophys Acta ; 1151(2): 223-36, 1993 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-8104034

RESUMO

Myocardial pyruvate oxidation is work- or calcium-load-related, but control of pyruvate dehydrogenase (PDH) by the specific mitochondrial pyruvate transporter has also been proposed. To test the transport hypothesis distribution of pyruvate across the cell membrane as well as rates of mitochondrial pyruvate net transport plus oxidation were examined in isolated perfused but stable and physiologically working guinea-pig hearts. 150 microM-1.2 mM alpha-cyanohydroxycinnamate proved to specifically block mitochondrial pyruvate uptake in these hearts. When perfusate glucose as cytosolic pyruvate precursor was supplied in combination with octanoate (0.2 or 0.5 mM) as diffusible alternative fatty acid substrate, alpha-cyanohydroxycinnamate produced up to 20- and 3-fold increases in pyruvate and lactate efflux, respectively. Cinnamates did not alter myocardial hemodynamics nor sarcolemmal pyruvate and lactate export. In contrast the tested concentrations of cinnamate produced reversible, dose-dependent decreases in 14CO2 production from [1-14C]pyruvate or [U-14C]glucose by inhibiting mitochondrial pyruvate uptake. Linear least-squares estimates of available cinnamate-sensitive total pyruvate transport potential yielded rates close to 110 mumol/min per g dry mass at S0.5 approximately 120 microM, which compared reasonably well with literature values from isolated cardiac mitochondria. This transport potential was severalfold larger than total extractable myocardial PDH activity of approximately 32 mumol/min per g dry mass at 37 degrees C. Even when cytosolic pyruvate levels were in the lower physiologic range of about 90 microM, pyruvate oxidation readily kept pace with mitochondrial respiration over a wide range of workload and inotropism. Furthermore, dichloroacetate, a selective activator of PDH, stimulated pyruvate oxidation without affecting myocardial O2 consumption, regardless of the metabolic or inotropic state of the hearts. Consequently, little or no regulatory function with regard to pyruvate oxidation could be assigned to the native mitochondrial pyruvate carrier of the working heart. Therefore, mitochondrial pyruvate-H+ symport was the normal, highly efficient (rather than controlling) mechanism for pyruvate entry into the mitochondria where PDH regulation controlled pyruvate oxidation.


Assuntos
Proteínas de Transporte/metabolismo , Proteínas de Membrana Transportadoras , Mitocôndrias Cardíacas/metabolismo , Complexo Piruvato Desidrogenase/metabolismo , Piruvatos/metabolismo , Função Ventricular , Agonistas Adrenérgicos/farmacologia , Animais , Transporte Biológico/efeitos dos fármacos , Radioisótopos de Carbono , Ácidos Cumáricos/antagonistas & inibidores , Ácidos Cumáricos/farmacologia , Ácido Dicloroacético/farmacologia , Glucose/metabolismo , Cobaias , Concentração de Íons de Hidrogênio , Cinética , Transportadores de Ácidos Monocarboxílicos , Oxirredução , Ácido Pirúvico , Sarcolema/metabolismo
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