Aspiration pneumonia with adenovirus infection in a Japanese macaque (Macaca fuscata fuscata).

Pneumonia with aspiration of foreign bodies and adenovirus infection was identified in a wild Japanese macaque which died 1 month after capture. The adenovirus infection was considered to be secondary in importance to the pneumonia.

[Electron microscopic study of the genome homology of simian adenoviruses]. / Elektronno-mikroskopicheskoe izuchenie gomologii genomov nekotorykh adenvirusov obez'ian.

DNA homologies of simian adenoviruses SA7, SV20, SV30, SV38, and SA7(C8), clone 230, were studied. The genomes of SV20, SV30, and SV38 differ from each other insignificantly (at least 85% of homology), but from the SA7 genome significantly (50-70% of homology). DNAs of SV20, SV30, and SV38 contain four main regions of melting in 90% formamide: two terminal A-T-rich regions are located symmetrically on the termini of the molecules, and two on the right parts of the molecules. SA7 virus DNA differs from that of the three above-mentioned viruses by the A-T composition and contains only two regions of melting on the right part of the molecule under similar denaturating conditions. SV20, SV30, and SV38 DNAs contain the inverted terminal repetitions of the order of 147 nucleotides long, and SA7 DNA contains considerably longer inverted terminal repetition: 230 nucleotides.

Oncogenicity of heat-inactivated simian adenovirus SA7.

Simian adenovirus SA7 heated for 30 minutes at 70 degrees C retained part of its ability to induce tumors in newborn hamsters. Tumors were also induced by DNA extracted from the heated virus. However, neither residual replicating, i.e. cytopathogenic, nor transforming and T-antigen-inducing activities could be detected in vitro in CV-1 and hamster embryo cells inoculated with the heated virus. The results are discussed in terms of a possible release of viral nucleic acid at the high temperature. They also show that current tests for effective virus inactivation based exclusively on the recognition of replicating virus should be reevaluated.

[Physical characteristics of simian adenovirus SA7]. / Fizicheskie kharakteristiki adenovirusa obez'ian SA7.

Physical characteristics of simian adenovirus SA7 virion were studied. The buoyant density of SA7 virions was found to be 1.356 g/cm3. The percentage of DNA calculated from the buoyant density value and determined with phosphorus directly was 11.6% and 11.8%, respectively. The S020,w and D020,w were 740 S and 3.34 x 10(-8)/cm2, respectively. From the hydrodynamic parameters of the virion and its volume and specific partial volume as well as percentage of DNA and DNA molecular weight, the molecular weight of SA7 virion was calculated and found to be 185.5 Mdaltons.

[Simian adenovirus type 7 (SA7) DNA fragmentation by specific R.Bgl II and R.Hind III endonucleases]. / Fragmentatsiia DNK adenovirusa obez'ian tipa 7 (SA7) spetsificheskimi éndonukleazami R.Bgl II i R.Hind III.

The effect of restricting R. BgIII and R. HindIII endonucleases on the genome of simian adenovirus type 7 (SA7) was studied. The R. BgIII endonuclease has 5 restriction sites on SA7 DNA and produces 6 fragments with molecular weights of 10.0 X 10(6), 5,3 X 10(6), 3.6 X 10(6), 1.8 X 10(6), 0.6 X 10(6), and 0.5 X 10(6) daltons. R. HindIII, having 3 restriction sites, hydrolyses SA7 DNA into 4 fragments with molecular weights of 7.6 X 10(6), 5.9 X 10(6), 5,2 X 10(6), 3,1 X 10(6) daltons. The R. BgIII and R. HindIII fragments are arranged in the SA7 genome in the following order: B-A-C-E-D-F and C-D-B-A.

[Electron microscopic study of genome homology in 2 clones of the simian adenoviruses type 7]. / Elektronno-mikroskopicheskoe izuchenie gomologii genomov dvukh klonov adenovirusov obez'ian tipa 7.

Homology of the genomes of large-plaque (lp) and small-plaque (sp) clones of simian adenovirus type 7 (SV7) was studied experimentally. Guanidine chloride helped to isolate circular DNA molecules of identical length for both clones within the measurement error (1 = 11 +/- 0.2 micrometer). A heteroduplex SV7lp--SV7sp molecule was found to have two nonhomology sites 0.5 +/- 0.3 mu and 1.0 +/- 0.5 micrometer long located at a distance of 2 and 4 micrometer from one of the ends of the molecules, respectively.