RESUMO
Urinary and serum colony-stimulating factor (CSF) levels were measured in 11 patients with chronic idiopathic neutropenia without infections and in 10 normal individuals. Urinary CSF output was determined using mouse marrow target cells, and serum CSF activity was assayed with human marrow target cells by the double agar layer technique. Using these methods, there was no significant difference between CSF levels of neutropenic and normal subjects. These data indicate that CSF levels are not inversely related to the blood neutrophil count in chronic idiopathic neutropenia and suggest that CSF is not a hormone regulating the blood neutrophil count in a manner analogous to the erythropoietin regulation of circulating erythrocyte levels.
Assuntos
Agranulocitose/urina , Fatores Estimuladores de Colônias/urina , Glicoproteínas/urina , Neutropenia/urina , Doença Crônica , Fatores Estimuladores de Colônias/sangue , Humanos , Técnicas In Vitro , Neutropenia/sangueAssuntos
Hematopoese , Leucócitos , Doença Aguda , Agranulocitose/urina , Anemia Aplástica/urina , Animais , Infecções Bacterianas/sangue , Medula Óssea/metabolismo , Células da Medula Óssea , Divisão Celular , Retroalimentação , Humanos , Leucemia/urina , Leucócitos/metabolismo , Leucócitos/fisiologia , Camundongos , Monócitos/metabolismo , Fatores de TempoRESUMO
The urinary activities for bone marrow colony formation were measured on consecutive 24-hour urine samples from two gray collie dogs with cyclic neutropenia and from two normal collies. The activity varied cyclically in the gray collies with a peak activity developing during the neutropenic phase, which antecedes the return of blood neutrophils. The activity fell to undetectable levels after the blood neutrophil counts returned to the normal range. The urine of normal dogs showed no activity. Since the dogs with cyclic neutropenia have been shown to have periodic hematopoiesis, these data suggest a regulatory hormonal role for the substance measured by this assay.
