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1.
Ecotoxicol Environ Saf ; 207: 111191, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-32890947

RESUMO

To elucidate the features of bioaccumulation and phytotoxic effects of long-lived artificial radionuclides, a hydroponic experiment was carried out with the cultivation of onion (Allium cepa L.) in low-mineralized solutions spiked with 137Cs (250 kBq L-1) or 243Am (9 kBq L-1). After the 27-day growth period, ≈70% of 137Cs and ≈14% of 243Am were transferred from the solutions to onion biomass with transfer factor values ≈ 400 and ≈ 80, respectively. Since the bioaccumulation of both radionuclides mainly took place in the roots of onion (77% 137Cs and 93% 243Am of the total amount in biomass), edible organs - bulbs and leaves - were protected to some extent from radioactive contamination. At the same time, the incorporation of the radionuclides into the root tissues caused certain changes in their biometric (geometric and mass) traits, which were more pronounced under the 243Am-treatment of onion. Exposure to 243Am significantly reduced the number, length, and total surface area of onion roots by 1.3-2.6 times. Under the influence of 137Cs, the dry-matter content in roots decreased by 1.3 times with a corresponding increase in the degree of hydration of the root tissues. On the whole, the data obtained revealed the specific features of 137Cs and 243Am behaviour in "hydroponic solution - plant" system and suggested that biometric traits of onion roots could be appropriate indicators of phyto(radio)toxicity.


Assuntos
Amerício/toxicidade , Radioisótopos de Césio/toxicidade , Cebolas/efeitos da radiação , Biomassa , Hidroponia , Cebolas/crescimento & desenvolvimento , Folhas de Planta , Raízes de Plantas/crescimento & desenvolvimento
2.
Health Phys ; 114(3): 288-298, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29360707

RESUMO

Distribution, retention, and excretion of intramuscularly injected Am citrate have been investigated in cynomolgus and rhesus nonhuman primates (NHP). Bioassay and retention data, obtained from experiments done by Patricia Durbin and her colleagues at Lawrence Berkeley National Laboratory, were evaluated against the International Commission on Radiological Protection (ICRP 67) Am systemic model coupled with to the National Council on Radiation Protection and Measurement wound model (NCRP 156). The default transfer rates suggested in these models were used with the urine and feces excretion data to predict the intake as well as liver and skeleton tissue contents at the time of death. The default models adequately predict the animals' urine bioassay data, but the injected activities were overpredicted by as much 4.41 times and underpredicted by as much as 0.99 times. Poor prediction has been observed in all cases using fecal excretion. The retained activity in the liver and skeleton were investigated using the same approach. It appears that the models predict the amount of the activity retention in the skeleton more accurately than in the liver. The fraction of predicted to measured activity at the time of death in the skeleton was over 1.0 in most cases, and accurate predictions were obtained in seven cases. The predicted activity in skeleton for these cases ranged from 2.7 to 17% overestimated activity and from 9 to 14% underestimated activity. NHPs' urine data and organ retention were compared with data from previously modeled baboons and beagle dogs. About 6% of the injected activity in baboons and beagle dog was excreted in urine and approximately 0.1% in feces in the first 24 h. The results from NHP are not different from excreta analysis in these other species. Urinary excretion in the cynomolgus, rhesus, and baboon NHP is the dominant pathway of Am clearance; however, fecal excretion is considered dominant in beagle dogs. The comparison between NHPs and humans is difficult due to the differences in the number of activities translocated or deposited in the liver tissue and nonliver tissues (primarily skeleton), in addition to the physiological differences between the NHPs and humans.


Assuntos
Amerício/farmacocinética , Modelos Biológicos , Lesões por Radiação/fisiopatologia , Proteção Radiológica/normas , Ferimentos e Lesões/fisiopatologia , Amerício/toxicidade , Animais , Bioensaio , Simulação por Computador , Cinética , Macaca fascicularis , Macaca mulatta , Taxa de Depuração Metabólica , Lesões por Radiação/etiologia , Medição de Risco , Distribuição Tecidual , Ferimentos e Lesões/etiologia
3.
Chem Biol Interact ; 267: 40-47, 2017 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-27234047

RESUMO

Skin contamination is one of the most probable risks following major nuclear or radiological incidents. However, accidents involving skin contamination with radionuclides may occur in the nuclear industry, in research laboratories and in nuclear medicine departments. This work aims to measure the penetration of the radiological contaminant Americium (241Am) in fresh and frozen skin and to evaluate the distribution of the contamination in the skin. Decontamination tests were performed using water, Fuller's earth and diethylene triamine pentaacetic acid (DTPA), which is the recommended treatment in case of skin contamination with actinides such as plutonium or americium. To assess these parameters, we used the Franz cell diffusion system with full-thickness skin obtained from pigs' ears, representative of human skin. Solutions of 241Am were deposited on the skin samples. The radioactivity content in each compartment and skin layers was measured after 24 h by liquid scintillation counting and alpha spectrophotometry. The Am cutaneous penetration to the receiver compartment is almost negligible in fresh and frozen skin. Multiple washings with water and DTPA recovered about 90% of the initial activity. The rest remains fixed mainly in the stratum corneum. Traces of activity were detected within the epidermis and dermis which is fixed and not accessible to the decontamination.


Assuntos
Amerício/toxicidade , Pele/efeitos dos fármacos , Compostos de Alumínio/química , Amerício/química , Animais , Autorradiografia , Descontaminação , Congelamento , Compostos de Magnésio/química , Ácido Pentético/química , Silicatos/química , Pele/metabolismo , Pele/patologia , Suínos
4.
Radiat Res ; 185(6): 568-79, 2016 06.
Artigo em Inglês | MEDLINE | ID: mdl-27195610

RESUMO

Diethylenetriaminepentaacetic acid (DTPA) is currently still the only known chelating drug that can be used for decorporation of internalized plutonium (Pu) and americium (Am). It is generally assumed that chelation occurs only in biological fluids, thus preventing Pu/Am deposition in target tissues. We postulate that actinide chelation may also occur inside cells by a mechanism called "intracellular chelation". To test this hypothesis, rats were given DTPA either prior to (termed "prophylactic" treatment) or belatedly after (termed "delayed" treatment) Pu/Am injection. DTPA decorporation efficacy was systematically tested for both plutonium and americium. Both prophylactic and delayed DTPA elicited marked decreases in liver Pu/Am. These results can be explained by chelation within subcellular compartments where DTPA efficacy increased as a function of a favorable intracellular DTPA-to-actinide molar ratio. The efficacy of intracellular chelation of liver actinides decreased with the delay of treatment. This is probably explained by progressive actinide binding to the high-affinity ligand ferritin followed by migration to lysosomes. Intracellular chelation was reduced as the gap between prophylactic treatment and contamination increased. This may be explained by the reduction of the intracellular DTPA pool, which declined exponentially with time. Skeletal Pu/Am was also reduced by prophylactic and delayed DTPA treatments. This decorporation of bone actinides may mainly result from extracellular chelation on bone surfaces. This work provides converging evidence for the involvement of an intracellular component of DTPA action in the decorporation process. These results may help to improve the interpretation of biological data from DTPA-treated contamination cases and could be useful to model DTPA therapy regimens.


Assuntos
Amerício/metabolismo , Quelantes/metabolismo , Espaço Intracelular/efeitos dos fármacos , Espaço Intracelular/metabolismo , Ácido Pentético/metabolismo , Plutônio/metabolismo , Amerício/isolamento & purificação , Amerício/toxicidade , Animais , Osso e Ossos/efeitos dos fármacos , Osso e Ossos/metabolismo , Quelantes/farmacologia , Relação Dose-Resposta a Droga , Cinética , Fígado/efeitos dos fármacos , Fígado/metabolismo , Masculino , Ácido Pentético/farmacologia , Plutônio/isolamento & purificação , Plutônio/toxicidade , Ratos , Ratos Sprague-Dawley
5.
J Environ Radioact ; 149: 51-63, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26204519

RESUMO

Human activity has led to an increasing amount of radionuclides in the environment and subsequently to an increased risk of exposure of the biosphere to ionising radiation. Due to their high linear energy transfer, α-emitters form a threat to biota when absorbed or integrated in living tissue. Among these, (241)Am is of major concern due to high affinity for organic matter and high specific activity. This study examines the dose-dependent biological effects of α-radiation delivered by (241)Am at the morphological, physiological and molecular level in 14-day old seedlings of Arabidopsis thaliana after hydroponic exposure for 4 or 7 days. Our results show that (241)Am has high transfer to the roots but low translocation to the shoots. In the roots, we observed a transcriptional response of reactive oxygen species scavenging and DNA repair pathways. At the physiological and morphological level this resulted in a response which evolved from redox balance control and stable biomass at low dose rates to growth reduction, reduced transfer and redox balance decline at higher dose rates. This situation was also reflected in the shoots where, despite the absence of a transcriptional response, the control of photosynthesis performance and redox balance declined with increasing dose rate. The data further suggest that the effects in both organs were initiated in the roots, where the highest dose rates occurred, ultimately affecting photosynthesis performance and carbon assimilation. Though further detailed study of nutrient balance and (241)Am localisation is necessary, it is clear that radionuclide uptake and distribution is a major parameter in the global exposure effects on plant performance and health.


Assuntos
Partículas alfa/efeitos adversos , Amerício/toxicidade , Antioxidantes/efeitos da radiação , Arabidopsis/efeitos da radiação , Dano ao DNA , Transcrição Gênica/efeitos da radiação , Antioxidantes/metabolismo , Arabidopsis/crescimento & desenvolvimento , Arabidopsis/metabolismo , Reparo do DNA/efeitos da radiação , Relação Dose-Resposta à Radiação , Estresse Oxidativo/efeitos da radiação , Fotossíntese/efeitos da radiação , Raízes de Plantas/metabolismo , Raízes de Plantas/efeitos da radiação , Brotos de Planta/metabolismo , Brotos de Planta/efeitos da radiação
6.
J Environ Radioact ; 142: 68-77, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25644753

RESUMO

The paper summarizes studies of effects of alpha- and beta-emitting radionuclides (americium-241, uranium-235+238, and tritium) on marine microorganisms under conditions of chronic low-dose irradiation in aqueous media. Luminous marine bacteria were chosen as an example of these microorganisms; bioluminescent intensity was used as a tested physiological parameter. Non-linear dose-effect dependence was demonstrated. Three successive stages in the bioluminescent response to americium-241 and tritium were found: 1--absence of effects (stress recognition), 2--activation (adaptive response), and 3--inhibition (suppression of physiological function, i.e. radiation toxicity). The effects were attributed to radiation hormesis phenomenon. Biological role of reactive oxygen species, secondary products of the radioactive decay, is discussed. The study suggests an approach to evaluation of non-toxic and toxic stages under conditions of chronic radioactive exposure.


Assuntos
Amerício/toxicidade , Bactérias/efeitos da radiação , Trítio/toxicidade , Urânio/toxicidade , Poluentes Radioativos da Água/toxicidade , Amerício/metabolismo , Bactérias/metabolismo , Relação Dose-Resposta à Radiação , Hormese , Água do Mar/microbiologia , Trítio/metabolismo , Urânio/metabolismo , Poluentes Radioativos da Água/metabolismo
8.
Int J Hyg Environ Health ; 215(3): 339-44, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22153871

RESUMO

Radon ((222)Rn) gas produces decay progeny that emits high energy alpha (α)-particles. Epidemiological studies have shown that exposure to (222)Rn is linked with elevated risk of developing lung cancer, however clear mechanisms leading to such effects have not been delineated. Cytokines play a critical role in inflammation and their dysregulated production often contributes to disease pathogenesis. In this study, Bio-plex multiplex technology was employed to investigate modulations of 27 pro-inflammatory cytokines following exposure of human monocytic cells to 1.5 Gy of α-particle radiation. Concurrently, DNA damage was assessed by examining the formation of phosphorylated H2A histone family X (γ-H2AX) sites. Of the 27 cytokines assessed, 4 cytokines were shown to be statistically downregulated by ∼2 fold relative to the untreated controls and included the interleukin (IL) family of proteins (IL-2, IL-15 and IL-17) and macrophage inflammatory protein 1 beta (MIP-1b). Interferon-inducible protein-12 (IP-12), vascular endothelial growth factor and regulated on activation normal T cell expressed and secreted (RANTES) were shown to be high expressors and upregulated. Cells irradiated with α-particles ranging from 0.27 to 2.14 Gy showed statistically significant, dose-dependant increases in γ-H2AX formation. These data suggest that α-particle radiation causes dysregulation in the production of a number of pro-inflammatory cytokines and results in significant DNA damage.


Assuntos
Partículas alfa , Exposição Ambiental , Amerício/toxicidade , Células Cultivadas , Dano ao DNA , Histonas/química , Histonas/genética , Histonas/metabolismo , Humanos , Fosforilação/efeitos da radiação
9.
J Environ Radioact ; 102(4): 407-11, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21388726

RESUMO

The effect of americium-241 ((241)Am), an alpha-emitting radionuclide of high specific activity, on luminous bacteria Photobacterium phosphoreum was studied. Traces of (241)Am in nutrient media (0.16-6.67 kBq/L) suppressed the growth of bacteria, but enhanced luminescence intensity and quantum yield at room temperature. Lower temperature (4 °C) increased the time of bacterial luminescence and revealed a stage of bioluminescence inhibition after 150 h of bioluminescence registration start. The role of conditions of exposure the bacterial cells to the (241)Am is discussed. The effect of (241)Am on luminous bacteria was attributed to peroxide compounds generated in water solutions as secondary products of radioactive decay. Increase of peroxide concentration in (241)Am solutions was demonstrated; and the similarity of (241)Am and hydrogen peroxide effects on bacterial luminescence was revealed. The study provides a scientific basis for elaboration of bioluminescence-based assay to monitor radiotoxicity of alpha-emitting radionuclides in aquatic solutions.


Assuntos
Amerício/toxicidade , Peróxido de Hidrogênio/metabolismo , Proteínas Luminescentes/metabolismo , Photobacterium/efeitos da radiação , Monitoramento de Radiação/métodos , Densitometria , Peróxido de Hidrogênio/toxicidade , Cinética , Medições Luminescentes , Photobacterium/crescimento & desenvolvimento , Temperatura
10.
J Environ Radioact ; 102(6): 543-50, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21392867

RESUMO

In order to understand the bioaccumulation of (241)Am and (134)Cs in scallops living in sediments, the uptake and depuration kinetics of these two elements were investigated in the king scallop Pecten maximus exposed via seawater, food, or sediment under laboratory conditions. Generally, (241)Am accumulation was higher and its retention was stronger than (134)Cs. This was especially obvious when considering whole animals exposed through seawater with whole-body concentration factors (CF(7d)) of 62 vs. 1, absorption efficiencies (A(0l)) of 78 vs. 45 for seawater and biological half-lives (T(b½l)) of 892 d vs. 22 d for (241)Am and (134)Cs, respectively. In contrast, following a single feeding with radiolabelled phytoplankton, the assimilation efficiency (AE) and T(b½l) of (134)Cs were higher than those of (241)Am (AE: 28% vs. 20%; T(b½l): 14 d vs. 9 d). Among scallop tissues, the shells always contained the higher proportion of the total body burden of (241)Am whatever the exposure pathway. In contrast, the whole soft parts presented the major fraction of whole-body burden of (134)Cs, which was generally associated with muscular tissues. Our results showed that the two radionuclides have contrasting behaviors in scallops, in relation to their physico-chemical properties.


Assuntos
Amerício/toxicidade , Radioisótopos de Césio/toxicidade , Pecten/efeitos dos fármacos , Poluentes Radioativos da Água/farmacocinética , Amerício/farmacocinética , Animais , Radioisótopos de Césio/farmacocinética , Cadeia Alimentar , França , Sedimentos Geológicos/análise , Meia-Vida , Cinética , Pecten/metabolismo , Água do Mar/análise
11.
Health Phys ; 99(3): 408-12, 2010 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-20699705

RESUMO

Commonly used water-soluble polyaminocarboxylic acid (PACA) chelators, such as EDTA and DTPA, require intravenous or subcutaneous administration due to their poor bioavailability. The bioavailability of PACAs can be improved by the addition of differing lengths of alkyl side chains that alter amphipathic properties. Orally administered amphipathic triethylenetetramine pentaacetic acid (TT) compounds are efficacious for decorporation of plutonium and americium. The synthesis, efficacy, binding affinities, and some initial pharmacokinetics properties of amphipathic TT chelators are reviewed. C-labeled C12TT and C22TT chelators are reasonably well absorbed from the intestine and have a substantial biliary/fecal excretion pathway, unlike DTPA, which is mostly excreted in the urine. Whole body retention times are increased as a function of increasing lipophilicity. Neutron-induced autoradiography studies demonstrate that the oral administration of the chelators can substantially inhibit the redistribution of Pu in skeletal tissues. In summary, amphipathic TT-based chelators have favorable bioavailability, have a significant biliary excretion pathway, have demonstrated efficacy for americium and plutonium, and are thus good candidates for further development. Furthermore, some of the pharmacological properties can be manipulated by changing the lengths of the alkyl side chains and this may have some advantage for decorporation of certain metals and radionuclides.


Assuntos
Acetatos/farmacologia , Acetatos/farmacocinética , Amerício/farmacocinética , Quelantes/farmacologia , Quelantes/farmacocinética , Descontaminação/métodos , Plutônio/farmacocinética , Trientina/análogos & derivados , Acetatos/administração & dosagem , Acetatos/química , Administração Oral , Amerício/toxicidade , Autorradiografia , Disponibilidade Biológica , Radioisótopos de Carbono , Quelantes/administração & dosagem , Quelantes/síntese química , Absorção Intestinal/efeitos dos fármacos , Absorção Intestinal/fisiologia , Ácido Pentético/química , Ácido Pentético/farmacologia , Plutônio/toxicidade , Trientina/administração & dosagem , Trientina/química , Trientina/farmacocinética , Trientina/farmacologia
12.
Sci Total Environ ; 408(12): 2567-75, 2010 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-20334902

RESUMO

There has been a decline in the population of some bird species at Morecambe Bay and the Solway Firth Special Protected Areas in North-West England during the last fifty years. It was suggested that the declines were caused, in part, by contaminants in the food and environment, primarily from the radioactive effluent discharge from the Sellafield Ltd nuclear fuel reprocessing plant in Cumbria. This study analysed bird feathers and tissues, vegetation and sediment for radionuclides, metals and persistent organic compounds. The non-radionuclide results were all low compared to relevant action limits. The ERICA model was used with field data to estimate the radiological dose to birds from exposure to (137)Cs and (241)Am with results between 1.26 to 3.83 microGy h(-1), below the ERICA screening level of 10 microGy h(-1) and within the IAEA 40 microGy h(-1) guideline value below which potential adverse impacts on biota are unlikely. The study showed no link between bird population decline and anthropogenic discharges to the SPAs.


Assuntos
Aves/crescimento & desenvolvimento , Exposição Ambiental/análise , Poluentes Ambientais/análise , Radioisótopos/análise , Amerício/análise , Amerício/toxicidade , Animais , Comportamento Animal/efeitos dos fármacos , Comportamento Animal/efeitos da radiação , Aves/metabolismo , Radioisótopos de Césio/análise , Radioisótopos de Césio/toxicidade , Conservação dos Recursos Naturais , DDT/análise , Relação Dose-Resposta à Radiação , Inglaterra , Monitoramento Ambiental , Plumas/química , Plumas/metabolismo , Sedimentos Geológicos/química , Hexaclorocicloexano/análise , Modelos Teóricos , Plantas/química , Bifenilos Policlorados/análise , Dinâmica Populacional , Radioisótopos/toxicidade , Radiometria
13.
Aquat Toxicol ; 87(3): 146-56, 2008 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-18325610

RESUMO

A 70-day experiment was performed with Daphnia magna exposed to waterborne Am-241 on a range of concentrations (from 0.4 to 40 Bq ml(-1)) in order to test chronic effects of internal alpha irradiation on respiration, somatic growth and reproduction over three successive generations. Changes in Am-241 concentrations were followed in the water and in daphnid tissues, eggs and cuticles. Corresponding average dose rates of 0.3, 1.5 and 15 mGy h(-1) were estimated. This study confirmed that oxygen consumption increased significantly in the first generation (F0) after 6 days of exposure to a dose rate >or=1.5 mGy h(-1). Consequences were limited to a reduction in body length (5%) and dry mass of females (16%) and eggs (8%) after 23 days of exposure, while mortality and fecundity remained unaffected. New cohorts were started with neonates of broods 1 and 5, to examine potential consequences of the reduced mass of offspring for subsequent exposed generations. Results strongly contrasted with those observed in F0. At the highest dose rate, an early mortality of 38-90% affected juveniles while survivors showed delayed reproduction and reduced fecundity in F1 and F2. At 0.3 and 1.5 mGy h(-1), mortality ranged from 31 to 38% of daphnids depending on dose rate, but was observed only in generation F1 started with neonates of the brood 1. Reproduction was affected through a reduction in the proportion of breeding females, occurring in the first offspring generation at 1.5 mGy h(-1) (to 62% of total daphnids) and in the second generation at 0.3 mGy h(-1) (to 69% of total daphnids). Oxygen consumption remained significantly higher at dose rates >or=0.3 mGy h(-1) than in the control in almost every generation. Body size and mass continued decreasing in relation to dose rate, with a significant reduction in mass ranging from 15% at 0.3 mGy h(-1) to 27% at 15 mGy h(-1) in the second offspring generation.


Assuntos
Amerício/toxicidade , Daphnia/crescimento & desenvolvimento , Daphnia/efeitos da radiação , Exposição Ambiental , Poluentes Radioativos da Água/toxicidade , Partículas alfa , Amerício/farmacocinética , Animais , Peso Corporal/efeitos da radiação , Respiração Celular/efeitos da radiação , Daphnia/metabolismo , Daphnia/fisiologia , Relação Dose-Resposta à Radiação , Feminino , Masculino , Óvulo/efeitos da radiação , Consumo de Oxigênio/efeitos da radiação , Reprodução/efeitos da radiação , Análise de Sobrevida , Poluentes Radioativos da Água/farmacocinética , Irradiação Corporal Total
14.
Hemoglobin ; 32(1-2): 199-206, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-18274997

RESUMO

Inhalation therapy of diethylene-triamine-penta-acetate (DTPA) should be initiated immediately to workers who have significant incorporation of plutonium, americium or curium in the nuclear fuel reprocessing plant. A newly designed electric mesh nebulizer is a small battery-operated passive vibrating mesh device, in which vibrations in an ultrasonic horn are used to force drug solution through a mesh of micron-sized holes. This nebulizer enables DTPA administration at an early stage in the event of a radiation emergency from contamination from the above radioactive metals.


Assuntos
Quelantes/administração & dosagem , Ácido Pentético/administração & dosagem , Lesões por Radiação/tratamento farmacológico , Liberação Nociva de Radioativos , Administração por Inalação , Amerício/toxicidade , Protocolos Clínicos , Cúrio/toxicidade , Humanos , Nebulizadores e Vaporizadores , Plutônio/toxicidade , Centrais Elétricas , Poluentes Radioativos/toxicidade
15.
Appl Radiat Isot ; 66(5): 632-47, 2008 May.
Artigo em Inglês | MEDLINE | ID: mdl-18222696

RESUMO

The human and animal data on the biokinetics of (242)Cm and (244)Cm are reviewed and shown to be very similar to those for (241)Am. Liver and skeleton are the main organs of deposition and the retention of curium in the skeleton is very prolonged in all the species examined. Retention of both curium and americium in the liver appears to be species-dependent, being relatively rapidly removed from the liver of rats, and probably humans, but being tenaciously retained in dogs and some other species. The radiotoxicity of curium is also reviewed and it is shown that, as with (241)Am, lung and bone tumour induction are the major hazards from inhaled and systemically deposited (244)Cm. The use of chelating agents for the treatment of accidental contamination of the human body with (242,244)Cm is also discussed.


Assuntos
Amerício/farmacocinética , Amerício/toxicidade , Cúrio/farmacocinética , Cúrio/toxicidade , Animais , Feminino , Humanos , Troca Materno-Fetal , Gravidez , Distribuição Tecidual
16.
Aquat Toxicol ; 80(3): 228-36, 2006 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-17049381

RESUMO

Daphnids were chronically exposed to waterborne Am-241, an alpha-emitting radionuclide, ranging in concentration from 0.4 to 40 Bq ml(-1). Am-241 amounts were monitored in the medium, daphnid tissues and cuticles. Corresponding average dose rates of 0.02, 0.11 and 0.99 mGy h(-1) were calculated for whole organisms with internal alpha-radiation contributing 99% of total dose rates. Effects of internal alpha irradiation on respiration and ingestion rates, adult, egg and neonate individual dry masses, fecundity and larval resistance to starvation were examined in 23-day experiments. Daphnids showed increased respiratory demand after 23 days at the highest dose rate, suggesting increased metabolic cost of maintenance due to coping with alpha radiological stress. Although no effect was detected on ingestion rates between contaminated and control daphnids, exposure to dose rates of 0.11 mGy h(-1) or higher, resulted in a significant 15% reduction in body mass. Fecundity remained unchanged over the 23-day period, but individual masses of eggs and neonates were significantly smaller compared to the control. This suggested that increased metabolic expenditure in chronically alpha-radiated daphnids came at the expense of their energy investment per offspring. As a consequence, neonates showed significantly reduced resistance to starvation at every dose rate compared to the control. Our observations are discussed in comparison with literature results reported for cadmium, a chemical toxicant which affects feeding activity and strongly reduces individual energy uptake.


Assuntos
Partículas alfa/efeitos adversos , Amerício/toxicidade , Daphnia/efeitos da radiação , Exposição Ambiental , Fatores Etários , Amerício/farmacocinética , Animais , Peso Corporal/efeitos da radiação , Respiração Celular/efeitos da radiação , Meios de Cultura/análise , Daphnia/crescimento & desenvolvimento , Daphnia/fisiologia , Relação Dose-Resposta à Radiação , Ingestão de Alimentos/efeitos da radiação , Feminino , Óvulo/efeitos da radiação , Consumo de Oxigênio/efeitos da radiação , Reprodução/efeitos da radiação
17.
Radiat Prot Dosimetry ; 105(1-4): 521-5, 2003.
Artigo em Inglês | MEDLINE | ID: mdl-14535232

RESUMO

This study aimed to assess the efficacy of 3,4,3-LI(1,2-HOPO) for reducing uranium, plutonium and americium in rats after intramuscular injection of (U-Pu)O2 particles (MOX). Sixteen rats were contaminated by intramuscular injection of a 1 mg MOX suspension and then treated daily for 7 d with LIHOPO (30 or 200 micromol kg(-1)) or DTPA (30 micromol kg(-1)). LIHOPO was inefficient for removing Pu, Am and U from the wound site. However, it reduced Pu retention in carcass and liver by factors of 2 and 6 respectively, and Am retention in carcass and liver by factors of 10 and 30. In contrast, the effect of LIHOPO on U was to decrease the retention in kidneys by a factor of 75. These results confirm that LIHOPO is a good candidate for use after contamination with MOX, in combination with localised wound lavage or surgical treatment aimed at removing most of the contaminant at the wound site.


Assuntos
Amerício/toxicidade , Compostos Aza/administração & dosagem , Quelantes/administração & dosagem , Terapia por Quelação/métodos , Plutônio/toxicidade , Piridonas/administração & dosagem , Lesões por Radiação/tratamento farmacológico , Compostos de Urânio/toxicidade , Amerício/administração & dosagem , Amerício/farmacocinética , Animais , Descontaminação/métodos , Feminino , Injeções Intramusculares , Especificidade de Órgãos , Óxidos/administração & dosagem , Óxidos/farmacocinética , Óxidos/toxicidade , Plutônio/administração & dosagem , Plutônio/farmacocinética , Pós , Lesões por Radiação/etiologia , Lesões por Radiação/prevenção & controle , Ratos , Resultado do Tratamento , Compostos de Urânio/administração & dosagem , Compostos de Urânio/farmacocinética , Contagem Corporal Total/métodos , Ferimentos Penetrantes/complicações , Ferimentos Penetrantes/tratamento farmacológico
18.
Radiat Prot Dosimetry ; 105(1-4): 329-31, 2003.
Artigo em Inglês | MEDLINE | ID: mdl-14526980

RESUMO

Although numerous models have been developed for occupational and medical internal dosimetry, they may not be applicable to an accident situation. Published dose coefficients relate effective dose to intake, but if acute deterministic effects are possible, effective dose is not a useful parameter. Consequently, dose rates to the organs of interest need to be computed from first principles. Standard bioassay methods may be used to assess body contents, but, again, the standard models for bioassay interpretation may not be applicable because of the circumstances of the accident and the prompt initiation of decorporation therapy. Examples of modifications to the standard methodologies include adjustment of biological half-times under therapy, such as in the Goiania accident, and the same effect, complicated by continued input from contaminated wounds, in the Hanford 241Am accident.


Assuntos
Amerício/farmacocinética , Radioisótopos de Césio/farmacocinética , Exposição Ocupacional/análise , Lesões por Radiação/tratamento farmacológico , Lesões por Radiação/metabolismo , Liberação Nociva de Radioativos , Radiometria/métodos , Amerício/toxicidade , Carga Corporal (Radioterapia) , Brasil , Radioisótopos de Césio/toxicidade , Simulação por Computador , Humanos , Modelos Biológicos , North Carolina , Especificidade de Órgãos , Doses de Radiação , Lesões por Radiação/etiologia
19.
Tsitol Genet ; 37(4): 20-5, 2003.
Artigo em Ucraniano | MEDLINE | ID: mdl-14569619

RESUMO

Analysis of chromosome instability (CI) is of great importance in view of pollution of the environment by genotoxic factors. Frequency of aberrant cells, spectrum of chromosome aberrations, damages of aberrant cell and distribution of aberrations in the cells are the most conventional parameters of CI. We have carried out the comparative analysis of the frequency of aberrant cells and the dynamics of aberrant cell damages induced by different mutagenic factors (alpha-irradiation from 241Am, gamma-irradiation from 60Co and tioTEPA) in Allium-test. This comparative analysis denotes that the studied parameters have different dynamics characterizing different mechanisms of CI in Allium cepa L.


Assuntos
Aberrações Cromossômicas , Mutagênicos , Allium/efeitos dos fármacos , Allium/genética , Allium/efeitos da radiação , Partículas alfa/efeitos adversos , Amerício/toxicidade , Antineoplásicos Alquilantes/efeitos da radiação , Radioisótopos de Cobalto/efeitos da radiação , Relação Dose-Resposta a Droga , Relação Dose-Resposta à Radiação , Raios gama/efeitos adversos , Tiotepa/efeitos da radiação
20.
Radiats Biol Radioecol ; 42(6): 675-7, 2002.
Artigo em Russo | MEDLINE | ID: mdl-12530148

RESUMO

The purpose of this study was to elucidate the genome damage induced by 241Am-irradiation using different parameters of cytogenetic evaluation in Allium-test. The root tip cells test-system for the cytogenetic effects studying was used. 241AmCl3 of different concentrations was used (1.5 x 10(-9)-1.5 x 10(-7) g/l). Water solution-to-plant transfer factor for 241Am was found to be 0.18 +/- 0.04. The internal doses of 241Am accumulated during germination were 0.37-37.00 cGy. The impact of 241Am-irradiation was evaluated on the mitotic index (MI), the yield of aberrant anaphases (AA), the distribution of chromosome aberrations number in cells and the average level of lesion of aberrant cell (LAC). Probably all these parameters are differ in sensitivity to damage factor, but only some changes in MI was revealed. It is supposed, that the absence of any changes in the distribution of chromosome aberrations number in cells and the average level of LAC in 241Am-irradiated cells confirm the absence of significant 241Am-impact on chromosomes, as the alpha-irradiation should cause significant damages in chromosomes. Although solutions of 241Am were high-concentrated, the seedlings didn't accumulate high internal doses. It appears the distribution of 241Am is a significantly heterogeneous hence it is possible the absorbed doses in nuclei can't reach the level necessary for revealing of cytogenetic effects.


Assuntos
Allium/efeitos da radiação , Amerício/toxicidade , Aberrações Cromossômicas , Allium/genética , Genoma de Planta
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