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1.
J Physiol Pharmacol ; 70(4)2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31741461

RESUMO

Amygdalin is most commonly occurring cyanogenic glycoside. It is found in seeds of many plant species. Our study was aimed to reveal whether pure intramuscularly injected amygdalin or apricot seeds peroral exposure cause changes in bone microstructure of rabbits. Twenty clinically healthy 5 months-old male rabbits were segregated into five groups. Animals from groups A1 and A2 were intramuscularly injected with amygdalin at doses of 0.6 and 3 mg/kg b.w. daily for 28 days. The groups S1 and S2 received commercial feed for rabbits mixed with crushed bitter apricot seeds at doses of 60 and 300 mg/kg b.w. during 28 days. The control (C) group did not receive any amygdalin. Intramuscular and peroral amygdalin administration did not affect total body weight, femoral length and femoral weight of rabbits. Similarly, microcomputed tomography (3D analysis) has shown that amygdalin had insignificant effect on relative bone volume, bone mineral density, cortical bone thickness, bone surface, trabecular thickness, trabecular number, trabecular separation. However, histological (2D analysis) revealed evident changes in compact bone microstructure of amygdalin-exposed rabbits consistent with a different vascularization and changed biomechanical properties. We can conclude that subacute exposure to amygdalin (both intramuscular and peroral) at the doses used in our study influenced compact bone remodeling.


Assuntos
Amigdalina/administração & dosagem , Antineoplásicos Fitogênicos/administração & dosagem , Remodelação Óssea/efeitos dos fármacos , Fêmur/efeitos dos fármacos , Administração Oral , Animais , Fêmur/diagnóstico por imagem , Fêmur/patologia , Fêmur/fisiologia , Injeções Intramusculares , Masculino , Coelhos , Microtomografia por Raio-X
2.
Ann Anat ; 224: 124-132, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31100343

RESUMO

BACKGROUND: Autoimmune hepatitis (AIH) is an immune-mediated inflammation of the liver characterized by disorganized hepatic parenchyma and inflammatory cell infiltration. Although the increased incidence of AIH, the development of novel therapeutic strategies are impeded by the poor understanding of the accompanied detailed immunopathogenic changes. CD4+ T cells are key mediators of inflammatory cell infiltration in initial phases of liver injuries like AIH. The distribution of CD4+ cells and the histopathological changes accompanying Con A-induced AIH were investigated together with the postulated protective effect of Amygdalin (Amg.). MATERIALS AND METHODS: 30 adult male mice were divided into three groups; control, AIH and AIH-Amg. groups. AIH was induced by a single intravenous injection of Concanavalin A (Con A) (15 mg/kg). The AIH-Amg. group received Amg. 5 mg/kg intraperitoneally once a week for three weeks. Blood samples were examined for ALT and AST. MDA, SOD, and GSH were determined in hepatic homogenates. Liver section stained with hematoxylin and eosin, Masson trichrome and CD4+ immune stain were examined by light and electron microscopy. RESULTS: AIH group showed a significant increase in levels of ALT, AST and MDA and a significant decline in SOD and GSH compared to the controls. The liver tissue showed distorted hepatic architecture with intercellular hemorrhage, necrosis, and inflammatory cell infiltration. The area percent of CD4+ immune staining was significantly increased. Electron microscopic examination showed massive cellular degenerative changes. Amg. pretreatment in AIH-Amg. group significantly reversed these changes. CONCLUSION: AIH induced CD4+ cells infiltration in the liver with subsequent liver tissue damage. Amg. pretreatment inhibited CD4+ cell infiltration and protected the liver tissue. This finding suggests that Amg. could be a therapeutic agent in the management of AIH.


Assuntos
Amigdalina/administração & dosagem , Linfócitos T CD4-Positivos/efeitos dos fármacos , Hepatite Autoimune/tratamento farmacológico , Fígado/imunologia , Fígado/patologia , Alanina Transaminase/sangue , Análise de Variância , Animais , Aspartato Aminotransferases/sangue , Concanavalina A/efeitos adversos , Glutationa/metabolismo , Hepatite Autoimune/etiologia , Hepatite Autoimune/imunologia , Hepatite Autoimune/prevenção & controle , Imuno-Histoquímica , Fígado/efeitos dos fármacos , Fígado/ultraestrutura , Masculino , Malondialdeído/metabolismo , Camundongos , Mitógenos/efeitos adversos , Estresse Oxidativo , Superóxido Dismutase/metabolismo
3.
Complement Ther Med ; 43: 295-299, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30935547

RESUMO

The natural compound amygdalin has gained high popularity among tumor patients as a complementary or alternative treatment option. However, due to metabolization of amygdalin to cyanide (HCN) following oral consumption, there could be a high risk of lactic acidosis caused by cyanide intoxication. The present retrospective study was undertaken to evaluate cyanide blood and lactate plasma levels of tumor patients (n = 55) before and after intravenous (i.v.) amygdalin infusion. All patients had also continuously ingested amygdalin tablets (3 x 500 mg/day), excepting on the days of i.v. administration. Each patient received one to five intravenous amygdalin treatments. The time period between each i.v. application ranged between 4-6 days. The initial i.v. dose was 6 mg (n = 28), 9 mg (n = 1), 15 mg (n = 1) or 18 mg (n = 25). The mean cyanide blood level before i.v. amygdalin administration was 34.74 µg/L, which increased significantly to a mean value of 66.20 µg/L after i. v. amygdalin application. In contrast, lactate decreased significantly from 1266 µmol/L pre-infusion to 868 µmol/L post-infusion. Increasing i.v. amygdalin by 1 mg was also associated with a significant increase in the cyanide level, while the lactate blood level significantly decreased. This is the first study evaluating cyanide levels under conditions employed by amygdalin administrators, i.e. after chronic oral amygdalin intake and then again after a closely subsequent intravenous amygdalin administration. Since lactate decreased, whilst cyanide increased, it is concluded that elevation of cyanide does not induce metabolic acidosis in terms of an increased lactate level.


Assuntos
Amigdalina/administração & dosagem , Antineoplásicos Fitogênicos/administração & dosagem , Cianetos/sangue , Lactatos/sangue , Administração Intravenosa/métodos , Administração Oral , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias/sangue , Neoplasias/tratamento farmacológico , Estudos Retrospectivos
4.
Med Hypotheses ; 112: 1-3, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29447928

RESUMO

Prostate cancer is impacting many men globally. It is a disease that has no effective treatment is available in the market. The understanding of the biophysical and biochemical aspects of the disease and the mechanism that allow it to metastasize is key to finding an effect treatment. Maintenance or pretreatment drug as well as a post treatment drug can be effective to avoid or delay the disease from appearing. The polysaccharides and monosaccharides polymers combined with vitamins can be the ingredient to developing the treatment. There are many evidences that investigators examined the individual components of the therapy proposed but never a combination of all these therapies. The one item that is not discussed is how to formulate the ingredient into an effective form which is a proprietary work being conducted currently. Nevertheless, the hypothesis seems reasonable to us and worth sharing with the scientific community.


Assuntos
Adenocarcinoma/tratamento farmacológico , Amigdalina/farmacologia , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Matriz Extracelular/efeitos dos fármacos , Glicosaminoglicanos/farmacologia , Modelos Biológicos , Polissacarídeos/farmacologia , Próstata/efeitos dos fármacos , Neoplasias da Próstata/tratamento farmacológico , Adenocarcinoma/metabolismo , Adenocarcinoma/patologia , Adenocarcinoma/terapia , Amigdalina/administração & dosagem , Divisão Celular/efeitos dos fármacos , Combinação de Medicamentos , Sinergismo Farmacológico , Matriz Extracelular/metabolismo , Glicosaminoglicanos/administração & dosagem , Heparitina Sulfato/administração & dosagem , Heparitina Sulfato/farmacologia , Humanos , Injeções Intravenosas , Masculino , Miócitos de Músculo Liso/efeitos dos fármacos , Polissacarídeos/administração & dosagem , Próstata/metabolismo , Próstata/patologia , Neoplasias da Próstata/metabolismo , Neoplasias da Próstata/patologia , Neoplasias da Próstata/terapia , Vitamina A/administração & dosagem , Vitamina A/farmacologia , Vitamina E/administração & dosagem , Vitamina E/farmacologia
5.
Sci Rep ; 6: 31364, 2016 08 16.
Artigo em Inglês | MEDLINE | ID: mdl-27527657

RESUMO

Guizhi Fuling capsule (GZFL), a traditional Chinese medicine formulation, is widely used in China to relieve pain from dysmenorrhea and is now in a Phase II clinical trial in the USA. Due to the low exposure of the five main medicative ingredients (amygdalin, cinnamic acid, gallic acid, paeoniflorin and paeonol) of GZFL in human, a strategy was built to qualitatively and quantitatively identify the possible metabolites of GZFL and to describe the pharmacokinetic profiles of GZFL in human. In this strategy, LC-Q-TOF/MS was used to identify and structurally elucidate the possible metabolites of GZFL in vivo; and a time-based metabolite-confirming step (TBMCs) was used to confirm uncertain metabolites. The simultaneously quantitation results by LC-MS/MS showed low exposure of the five medicative ingredients. According to the strategy we built, a total of 36 metabolites were found and structurally elucidated. The simultaneously semi-quantitative analysis by LC-MS/MS showed that obvious time-concentration curves could be established for 12 of the metabolites, and most of them showed a relatively higher exposure. This study provides a better understanding of the metabolic processes of GZFL in human.


Assuntos
Medicamentos de Ervas Chinesas/farmacocinética , Acetofenonas/administração & dosagem , Acetofenonas/química , Acetofenonas/farmacocinética , Amigdalina/administração & dosagem , Amigdalina/química , Amigdalina/farmacocinética , Cápsulas , Cromatografia Líquida de Alta Pressão/métodos , Cinamatos/administração & dosagem , Cinamatos/química , Cinamatos/farmacocinética , Medicamentos de Ervas Chinesas/administração & dosagem , Medicamentos de Ervas Chinesas/química , Feminino , Ácido Gálico/administração & dosagem , Ácido Gálico/química , Ácido Gálico/farmacocinética , Glucosídeos/administração & dosagem , Glucosídeos/química , Glucosídeos/farmacocinética , Voluntários Saudáveis , Humanos , Masculino , Estrutura Molecular , Monoterpenos/administração & dosagem , Monoterpenos/química , Monoterpenos/farmacocinética , Espectrometria de Massas em Tandem/métodos
6.
J Tradit Chin Med ; 36(2): 238-42, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27400480

RESUMO

OBJECTIVE: To analyze the transdermal profile of pseudoephedrine and amygdalin in the Traditional Chinese Medicine majiepingchuan in rat skin and to reveal their interaction. METHODS: A Franz diffusion cell was used in vitro to evaluate the transdermal parameters of cumulative transdermal flux (Q(tot)), cumulative transmission (T(tot)), and mean penetration rate (Kp) of pseudoephedrine and amygdalin in majiepingchuan. Linear regression analyses of Q(tot) over time of pseudoephedrine vs amygdalin and their ratios was adopted for correlation evaluation. RESULTS: At 1, 2, 4, 6, and 8 h, the Q(tot), T(tot) and Kp of pseudoephedrine showed a good correlation with that of amygdalin. CONCLUSION: There was a small difference in the ratios of Q(tot), T(tot) and Kp between pseudoephedrine and amygdalin, and a correlation between them.


Assuntos
Amigdalina/farmacocinética , Medicamentos de Ervas Chinesas/farmacocinética , Pseudoefedrina/farmacocinética , Administração Cutânea , Amigdalina/administração & dosagem , Animais , Medicamentos de Ervas Chinesas/administração & dosagem , Masculino , Pseudoefedrina/administração & dosagem , Ratos , Ratos Sprague-Dawley , Pele/química , Pele/metabolismo
7.
Life Sci ; 147: 137-42, 2016 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-26827990

RESUMO

AIMS: Despite impressive survival benefits from new agents to treat metastasized prostate cancer (PCa), progressive drug resistance hinders long-term response and restricts the efficacy of subsequent therapy. Due to reported antitumor activity of amygdalin and growing popularity for complementary and alternative medicine the potential of this natural, widely used substance to exert antineoplastic effects on prostate cancer cells has been assessed. MAIN METHODS: LNCaP (castration-sensitive), DU-145 and PC3 cells (castration-resistant) were exposed to different concentrations of amygdalin for 24h or 2weeks. Cell growth was measured by the MTT test, clonal formation by the clonogenic assay. Flow cytometry served to investigate apoptosis and cell cycle phases. Cell cycle regulating proteins and the mTOR-akt signaling axis were analyzed by western blotting. KEY FINDINGS: Amygdalin dose-dependently diminished tumor cell growth with maximum effects at 10mg/ml. Apoptosis of PC3 and LNCaP but not of DU-145 cells was reduced, whereas colony formation was suppressed in all cell lines. A decrease in the number of G2/M- and S-phase cells along with an elevated number of G0/G1-phase cells was recorded. The cell cycle proteins cdk 1, cdk 2 and cdk 4 as well as cyclin A, cyclin B and cyclin D3 were modulated by amygdalin after both 24h and 2weeks. Distinct effects on p19 and p27 expression and on Akt, Rictor and Raptor activation became evident only after 2weeks. SIGNIFICANCE: Amygdalin exhibits significant antitumor activity in both castration-sensitive and castration-resistant PCa cell lines and merits further evaluation for therapeutic purposes.


Assuntos
Amigdalina/farmacologia , Antineoplásicos Fitogênicos/farmacologia , Apoptose/efeitos dos fármacos , Neoplasias de Próstata Resistentes à Castração/tratamento farmacológico , Neoplasias da Próstata/tratamento farmacológico , Amigdalina/administração & dosagem , Antineoplásicos Fitogênicos/administração & dosagem , Western Blotting , Ciclo Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Relação Dose-Resposta a Droga , Citometria de Fluxo , Humanos , Masculino , Neoplasias da Próstata/patologia , Neoplasias de Próstata Resistentes à Castração/patologia , Fatores de Tempo
8.
Clin Ther ; 38(2): 327-37, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26749220

RESUMO

PURPOSE: Huoxue-Tongluo lyophilized powder for injection (HTLPI), a traditional Chinese medicine preparation, is a compound of Persicae semen and Paeoniae Radix Rubra that is used mainly for treating blood-stasis obstruction syndrome in the acute stage of cerebral ischemic stroke. Amygdalin (AD) and paeoniflorin (PF) are 2 typical bioactive components in HTLPI and were selected as indicators for this pharmacokinetic study of HTLPI. The objective of this study was to investigate the safety profile, tolerability, and pharmacokinetic properties of AD and PF after single and multiple intravenous infusions of HTLPI in healthy Chinese volunteers. METHODS: Twenty-one healthy Chinese subjects were recruited for this open-label, single ascending-dose (3, 6, and 9 g) and multiple-dose (6 g, once daily) study. Safety profile was assessed by adverse events and physical examination throughout the study. Serial plasma and urine samples were analyzed by HPLC-MS/MS. Pharmacokinetic parameters of AD and PF were calculated using noncompartmental analysis. FINDINGS: In the single-dose phase of the study, the mean maximum plasma concentration and the mean area under the plasma concentration-time curve of AD and PF increased proportionally with each dose escalation. In the multiple-dose phase, the steady state was achieved by day 4 after multiple-dose administration of 6 g HTLPI. Mean pharmacokinetic parameters achieved on day 1 were similar to those on day 7. No significant accumulation was observed after repeat doses of 6 g HTLPI. Approximately 79.6% of the administered AD and 48.4% of the administered PF were excreted unchanged in urine within 24 hours. No serious adverse events were observed during the entire study. IMPLICATIONS: The pharmacokinetic properties of AD and PF were linear after a single intravenous infusion of HTLPI in the dose range of 3-9 g. No systemic accumulation was observed with repeat doses of HTLPI. Sex had no significant effect on the pharmacokinetic properties of AD and PF. Intravenous infusion of HTLPI was well tolerated in healthy Chinese subjects.


Assuntos
Amigdalina/administração & dosagem , Glucosídeos/administração & dosagem , Monoterpenos/administração & dosagem , Adulto , Amigdalina/efeitos adversos , Amigdalina/farmacocinética , Povo Asiático , Cromatografia Líquida de Alta Pressão , Medicamentos de Ervas Chinesas/administração & dosagem , Feminino , Glucosídeos/efeitos adversos , Glucosídeos/farmacocinética , Humanos , Infusões Intravenosas , Masculino , Monoterpenos/efeitos adversos , Monoterpenos/farmacocinética , Pós , Espectrometria de Massas em Tandem , Adulto Jovem
9.
Int J Mol Med ; 37(2): 526-32, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26709398

RESUMO

Although amygdalin is used by many cancer patients as an antitumor agent, there is a lack of information on the efficacy and toxicity of this natural compound. In the present study, the inhibitory effect of amygdalin on the growth of renal cell carcinoma (RCC) cells was examined. Amygdalin (10 mg/ml) was applied to the RCC cell lines, Caki-1, KTC-26 and A498, for 24 h or 2 weeks. Untreated cells served as controls. Tumor cell growth and proliferation were determined using MTT and BrdU tests, and cell cycle phases were evaluated. Expression of the cell cycle activating proteins cdk1, cdk2, cdk4, cyclin A, cyclin B, cyclin D1 and D3 as well as of the cell cycle inhibiting proteins p19 and p27 was examined by western blot analysis. Surface expression of the differentiation markers E- and N-cadherin was also investigated. Functional blockade by siRNA was used to determine the impact of several proteins on tumor cell growth. Amygdalin treatment caused a significant reduction in RCC cell growth and proliferation. This effect was correlated with a reduced percentage of G2/M-phase RCC cells and an increased percentage of cells in the G0/1-phase (Caki-1 and A498) or cell cycle arrest in the S-phase (KTC-26). Furthermore, amygdalin induced a marked decrease in cell cycle activating proteins, in particular cdk1 and cyclin B. Functional blocking of cdk1 and cyclin B resulted in significantly diminished tumor cell growth in all three RCC cell lines. Aside from its inhibitory effects on growth, amygdalin also modulated the differentiation markers, E- and N-cadherin. Hence, exposing RCC cells to amygdalin inhibited cell cycle progression and tumor cell growth by impairing cdk1 and cyclin B expression. Moreover, we noted that amygdalin affected differentiation markers. Thus, we suggest that amygdalin exerted RCC antitumor effects in vitro.


Assuntos
Amigdalina/administração & dosagem , Carcinoma de Células Renais/tratamento farmacológico , Ciclina B/biossíntese , Quinases Ciclina-Dependentes/biossíntese , Apoptose/efeitos dos fármacos , Proteína Quinase CDC2 , Carcinoma de Células Renais/genética , Carcinoma de Células Renais/patologia , Ciclo Celular/efeitos dos fármacos , Proteínas de Ciclo Celular/biossíntese , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Ciclina B/genética , Quinases Ciclina-Dependentes/genética , Regulação Neoplásica da Expressão Gênica , Humanos
10.
J Cancer Res Ther ; 10 Suppl 1: 3-7, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25207888

RESUMO

Malignant tumors are the major disease that cause serious damage to human health, and have been listed as the premier diseases which seriously threatened human health by World Health Organization (WHO). In recent years the development of antitumor drugs has been gradually transformed from cytotoxic drugs to improving the selectivity of drugs, overcoming multidrug resistance, development of new targeted drugs and low toxicity with high specificity drugs. Amygdalin is a natural product that owns antitumor activity, less side effects, widely sourced and relatively low priced. All these features make the amygdalin a promising antitumor drugs, if combined with conditional chemotherapy drugs, which can produce synergistic effect. In this paper, we summarized the pharmacological activity, toxicity and antitumor activity of amygdalin, mainly focused on the advanced research of amygdalin on its antitumor effects in recent years, providing new insights for the development of new anticancer drugs, new targets searching and natural antitumor mechanism investigations.


Assuntos
Amigdalina/administração & dosagem , Antineoplásicos/administração & dosagem , Neoplasias/tratamento farmacológico , Amigdalina/efeitos adversos , Amigdalina/química , Antineoplásicos/efeitos adversos , Antineoplásicos/química , Apoptose/efeitos dos fármacos , Humanos , Neoplasias/patologia
11.
Indian J Pathol Microbiol ; 55(2): 211-4, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22771646

RESUMO

BACKGROUND: Angiogenesis contributes to different physiological and pathological conditions. The aim of this study was to investigate for the first time the antiangiogenic effects of amygdalin on the cultured endothelial cells of diabetic rats. MATERIALS AND METHODS: A total of 20 streptozotocin-induced diabetic rats were divided into two equal groups of control and amygdalin-treated animals. Eight weeks after the induction of diabetes, amygdalin was injected intraperitoneally (3 mg/kg) to the rats of the treatment group. One day later, rats were sacrificed; the aortic arteries were excised and cut as 2 mm rings. Each aortic ring was incubated in a cell-culture well for 7 days. The process of angiogenesis was monitored by counting the number of microvessels and primary microtubules in each well. RESULTS: Optic microscopy showed proliferation and migration of new endothelial cells to the fibrin gels. The endothelial cells produced primary microtubules which gradually made several branches and finally made a vascular matrix. The number of the primary microtubules and microvessels were significantly lower in the amygdalin-treated vs. control group (P < 0.01). CONCLUSION: Therefore, amygdalin exerts inhibitory effects on angiogenesis in aortic rings of diabetic rats and may pave a new way for treatment of unfavorable angiogenic conditions.


Assuntos
Amigdalina/administração & dosagem , Cianetos/administração & dosagem , Células Endoteliais/efeitos dos fármacos , Neovascularização Fisiológica/efeitos dos fármacos , Animais , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Diabetes Mellitus Experimental , Masculino , Microscopia , Ratos , Ratos Sprague-Dawley
12.
Vopr Onkol ; 58(5): 698-704, 2012.
Artigo em Russo | MEDLINE | ID: mdl-23600293

RESUMO

Letril (amygdaline) is one of drugs of alternative therapy for cancer that is used over three decades and relates to cyanogenic glycosides received from kernels of various fruits (almonds, apricots, peaches, etc. The basis of suggestion of letril as antitumor agent is hypotheses about selective fermentative splitting of amygdaline in tumor cells with developing of cyanide that should cause to apoptosis as a result of aerobic glycolysis suppression. None of these assumptions found their experimental confirmation. In clinical trials there was established inefficiency of letril with a very high probability to develop severe cyanide intoxication. Despite obtained scientific data and absence of permission from the supervising institutions (FDA) letril is still advertised, produced and distributed as anti-tumor drug.


Assuntos
Amigdalina/efeitos adversos , Amigdalina/metabolismo , Antineoplásicos/efeitos adversos , Antineoplásicos/metabolismo , Cianetos/efeitos adversos , Neoplasias/tratamento farmacológico , Amigdalina/administração & dosagem , Animais , Antineoplásicos/administração & dosagem , Ensaios Clínicos como Assunto , Humanos , National Cancer Institute (U.S.) , Neoplasias/metabolismo , Nitrilas/efeitos adversos , Nitrilas/metabolismo , Falha de Tratamento , Estados Unidos
13.
Curr Biol ; 20(24): 2234-40, 2010 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-21129969

RESUMO

Avoiding toxins in food is as important as obtaining nutrition. Conditioned food aversions have been studied in animals as diverse as nematodes and humans [1, 2], but the neural signaling mechanisms underlying this form of learning have been difficult to pinpoint. Honeybees quickly learn to associate floral cues with food [3], a trait that makes them an excellent model organism for studying the neural mechanisms of learning and memory. Here we show that honeybees not only detect toxins but can also learn to associate odors with both the taste of toxins and the postingestive consequences of consuming them. We found that two distinct monoaminergic pathways mediate learned food aversions in the honeybee. As for other insect species conditioned with salt or electric shock reinforcers [4-7], learned avoidances of odors paired with bad-tasting toxins are mediated by dopamine. Our experiments are the first to identify a second, postingestive pathway for learned olfactory aversions that involves serotonin. This second pathway may represent an ancient mechanism for food aversion learning conserved across animal lineages.


Assuntos
Aprendizagem da Esquiva , Abelhas , Condicionamento Operante , Preferências Alimentares , Reforço Psicológico , Amigdalina/administração & dosagem , Animais , Dopamina/metabolismo , Eletrofisiologia , Humanos , Quinina/administração & dosagem , Receptores Acoplados a Proteínas G/metabolismo , Serotonina/metabolismo , Percepção Gustatória/fisiologia
14.
Zhongguo Zhong Xi Yi Jie He Za Zhi ; 30(1): 58-63, 2010 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-20353036

RESUMO

OBJECTIVE: To explore the method and significance for studying active anti-liver fibrosis ingredients consisted Chinese medicine compound prescription based on Chinese medicine theory. METHODS: Optimized prescription was screened out, adopting uniform block design with 4-factor 8-level table and regression analysis, through applying the four known effective ingredients (cordyceps sinensis polysaccharide, salvianolic acid B, amygdaloside and gypenosides) of Fuzheng Huayu Capsule (FZHYC, a new Chinese medine anti-liver fibrosis drug) to two rat liver fibrosis models established separately by dimethylnitrosamine (DMN) and CCl4, and taking the liver content of hydroxyproline (Hyp) as the screen index. Then a further study for comparing and verifying the efficacy of the obtained optimized prescription was conducted on the two former models respectively by observing the changes of Hyp content in liver, serum ALT activity and fibrosis pathology after medication, controlled by the original FZHYC and the recipe assembled by all the four ingredients. RESULTS: Two optimized prescriptions (OPA and OPB) were screened out separately in the studies conducted on the two models. Both of them were consisted of cordyceps sinensis polysaccharide, Amygdaloside and Gypenosides, but different in constituent ratio, i.e., the ratio in OPA was 60 : 80: 50, and that in OPB, 20: 160: 50. Verifying study showed both OPA and OPA were significantly effective, with the efficacy equivalent to that of FZHYC (P>0.05). However, when they were used in combining with salvianolic acid B (the cutout ingredient in the screening), the efficacy lowered surely. CONCLUSIONS: Uniform design is a valuable method in the compatibility research of Chinese Medicine drugs' composition. To assemble a new compound recipe reasonably based on the prescription of traditional compound recipe could make its effect equivalent to that of the original prescription. Ingredients or constituents in a prescription, either presented synergistic or antagonistic effects, are not randomly stacked together, and they should be orderly assembled in intrinsic rules of qualitative and quantitative changing.


Assuntos
Desenho de Fármacos , Medicamentos de Ervas Chinesas/administração & dosagem , Amigdalina/administração & dosagem , Animais , Combinação de Medicamentos , Medicamentos de Ervas Chinesas/uso terapêutico , Gynostemma , Cirrose Hepática/tratamento farmacológico , Masculino , Extratos Vegetais/administração & dosagem , Ratos , Ratos Wistar , Análise de Regressão
15.
Biol Pharm Bull ; 22(2): 165-71, 1999 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-10077436

RESUMO

Effects of ephedrine, amygdalin, glycyrrhizin, gypsum and their combinations on body temperature and body fluid were studied in rats using the method developed in our previous reports. Ephedrine significantly increased respiratory evaporative water loss and heat loss in response to a marked elevation of body temperature. There was a small but significant increase in body temperature when amygdalin was orally given rats at a dose of 46.32 mg/kg. Glycyrrhizin and gypsum were unable to affect body temperature. However, gypsum was able to prevent the increased action of ephedrine on body temperature, amygdalin exhibited a preventive tendency to it, and glycyrrhizin did not affect it. The results are in good agreement with classical claims of Makyo-kanseki-to and the related crude drugs in traditional medicine. Moreover, a combination of the four components reproduced the effects of Makyo-kanseki-to on body temperature and body fluid. This report suggests that the co-administration of ephedrine and gypsum is physiologically more desirable than ephedrine alone for dry-type asthmatic patients with a fever. Also, it experimentally supports the clinical efficacy of Makyo-kanseki-to.


Assuntos
Amigdalina/farmacologia , Sulfato de Cálcio/farmacologia , Efedrina/farmacologia , Ácido Glicirrízico/farmacologia , Medicina Tradicional Chinesa , Amigdalina/administração & dosagem , Animais , Líquidos Corporais/efeitos dos fármacos , Temperatura Corporal/efeitos dos fármacos , Sulfato de Cálcio/administração & dosagem , Combinação de Medicamentos , Efedrina/administração & dosagem , Ácido Glicirrízico/administração & dosagem , Masculino , Ratos , Ratos Wistar
16.
Int J Cancer ; 78(6): 712-9, 1998 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-9833764

RESUMO

We describe a novel version of antibody-directed enzyme prodrug therapy (ADEPT), with the use of amygdalin as prodrug. Amygdalin is a naturally occurring cyanogenic glycoside, which can be cleaved by sweet almond beta-glucosidase to yield free cyanide. If amygdalin could be activated specifically at the tumour site, then malignant cells would be killed without the systemic toxicity usually associated with chemotherapy. To this end, we have conjugated beta-glucosidase to a tumour-associated monoclonal antibody (MAb) (HMFG1) and the conjugate has been tested in vitro for specificity and cytotoxicity subsequent to activation of amygdalin. Amygdalin was cytotoxic to HT1376 bladder cancer cells only at high concentrations, whereas the combination of amygdalin with HMFG1-beta-glucosidase enhanced the cytotoxic effect of amygdalin by 36-fold. When 2 concentrations of HMFG1-beta-glucosidase were compared, the toxic effect was dose dependent. The cytotoxicity of amygdalin was also enhanced by the MAb-enzyme conjugate even when the unbound conjugate was removed from the medium prior to exposure to amygdalin and the cells were washed. In addition to the cytotoxic effect, we also demonstrated specificity, using a MAb-enzyme conjugate that does not recognise the HT1376 bladder cancer cells. Finally, we studied the cytotoxic effect of the conjugate in co-culture of HMFG1-positive and-negative cell lines (HT 1376 and U87MG cells). We demonstrated that the rate of surviving cells corresponds well to the percentage of U87MG (HMFG1-negative) cells in the flask. Our findings indicate that ADEPT is more effective than non-directed enzyme activation of a prodrug and can result in a non-toxic cancer therapy.


Assuntos
Amigdalina/administração & dosagem , Anticorpos Monoclonais/administração & dosagem , Pró-Fármacos/administração & dosagem , Neoplasias da Bexiga Urinária/tratamento farmacológico , beta-Glucosidase/administração & dosagem , Especificidade de Anticorpos , Antígenos de Neoplasias , Sobrevivência Celular , Ensaio de Imunoadsorção Enzimática , Humanos , Células Tumorais Cultivadas , Neoplasias da Bexiga Urinária/imunologia
17.
Can J Physiol Pharmacol ; 63(9): 1084-7, 1985 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-3931889

RESUMO

The organs of 15-day-old rats had the highest capability to hydrolyze amygdalin and prunasin, and most of this activity is concentrated in the tissues of the small and large intestines. The activity decreased with age. In adult rats, the ability of the organs to hydrolyze prunasin is higher than that of amygdalin and is concentrated in the spleen, large intestine, and kidney (35.0, 15.0, and 8.9 micrograms prunasin hydrolyzed . h-1 . g tissue-1). Minced tissues of the liver, spleen, kidney, and stomach contain more hydrolytic capability than the homogenate of these organs, while the reverse is the case with the small and large intestines. When 30 mg amygdalin was orally administered to adult rats, its distribution after the 1st h was as follows: stomach (0.89 mg), small intestine (0.78 mg), spleen (0.36 mg), large intestine (0.30 mg), kidney (0.19 mg), liver (0.10 mg), and serum (5.6 micrograms/mL). At the end of the 2nd h, the highest amygdalin content was found in the large intestine (0.79 mg).


Assuntos
Amigdalina/metabolismo , Glucosidases/metabolismo , beta-Glucosidase/metabolismo , Administração Oral , Envelhecimento , Amigdalina/administração & dosagem , Animais , Sistema Digestório/enzimologia , Sistema Digestório/crescimento & desenvolvimento , Rim/enzimologia , Rim/crescimento & desenvolvimento , Fígado/enzimologia , Fígado/crescimento & desenvolvimento , Ratos , Ratos Endogâmicos , Baço/enzimologia , Baço/crescimento & desenvolvimento
18.
J Cancer Res Clin Oncol ; 109(3): 208-9, 1985.
Artigo em Inglês | MEDLINE | ID: mdl-4008516

RESUMO

The anti-tumor activity of amygdalin (NSC 251222), commercially known as Laetrile, was investigated using P388 lymphocytic leukaemia and P815 mast-cell leukaemia in BDF1 mice. Doses varying from 200 mg/kg to 2,000 mg/kg were used following the days 1 and 5 and days 1, 5 and 9 schedules. Despite treatment with high doses of amygdalin there was no prolongation in the life-span of mice bearing either P388 or P815 tumor.


Assuntos
Amigdalina/administração & dosagem , Leucemia P388/tratamento farmacológico , Leucemia Experimental/tratamento farmacológico , Mastócitos , Animais , Camundongos , Camundongos Endogâmicos
19.
Arch Toxicol ; 49(3-4): 311-9, 1982 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-7092570

RESUMO

Amygdalin (D-mandelonitrile-beta-D-gentiobioside) is a cyanogenic glycoside claimed to show anti-cancer activity, sold under the incorrect name "Laetrile". For a sensible discussion of its alleged activity and its established toxicity it is necessary that its fate in the organism is known. The pharmacokinetics of amygdalin have been investigated in the Beagle dog after both intravenous and oral administration. The excretion of amygdalin has also been studied in the rat. Amygdalin concentrations were determined by high performance liquid chromatography in plasma ultrafiltrate and urine. The pharmacokinetics of amygdalin after intravenous administration were compared with those of diatrizoate, a model substance for extracellular volume and glomerular filtration. The amygdalin clearance is significantly larger than that of diatrizoate. The volumes of distribution of both substance are the same. After oral administration only a few percents of the amygdalin dose are systemically available. A part of the oral dose is recovered from the urine as prunasin (D-mandelonitrile-beta-D-glucoside).


Assuntos
Amigdalina/metabolismo , Administração Oral , Amigdalina/administração & dosagem , Animais , Diatrizoato/metabolismo , Cães , Feminino , Injeções Intravenosas , Cinética , Masculino , Ratos , Ratos Endogâmicos
20.
Science ; 215(4539): 1513-5, 1982 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-7063858

RESUMO

Laetrile administered orally ot pregnant hamsters caused skeletal malformations in the offspring, but intravenous laetrile filed to result in embryopathic effects. Oral laetrile significantly increased in situ cyanide concentrations, while intravenous laetrile did not. Thiosulfate administration protected embryos from the teratogenic effects of oral laetrile. The embryopathic effects of oral laetrile appear to be due to cyanide released by bacterial beta-glucosidase activity.


Assuntos
Anormalidades Induzidas por Medicamentos/etiologia , Amigdalina/toxicidade , Administração Oral , Amigdalina/administração & dosagem , Amigdalina/metabolismo , Animais , Cricetinae , Feminino , Injeções Intravenosas , Gravidez
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