RESUMO
5alpha-Androst-1-ene-3beta,17beta-diol (1) was detected in extracts from fat of Sus scrofa L. (pig) by comparison with the commercially available synthetic compound, using gas chromatography-mass spectrometry. This observation is unprecedented because 1 is currently sold as a nutritional supplement, yet has not been previously reported as naturally occurring in the food supply.
Assuntos
Androstenodiol , Suplementos Nutricionais , Lipídeos/química , Androstenodiol/química , Androstenodiol/isolamento & purificação , Androstenodiol/metabolismo , Animais , Abastecimento de Alimentos , Cromatografia Gasosa-Espectrometria de Massas , Estrutura Molecular , SuínosRESUMO
The metabolism of androgens in the testis of the lizard Tiliqua rugosa has been studied in vitro by incubating cellular homogenates with radiolabeled C19-steroid substrates. The identification 17 beta-oxidoreductase and 3 beta-hydroxysteroid dehydrogenase/isomerase activities. Aromatase, 5 alpha-reductase, and 17 alpha/beta-epimerase activities were not detected. The 17 alpha-oxidoreductase activity was temperature dependent (maximal at 32 degrees), while the 17 beta-oxidoreductase activity was temperature independent. Time yield and dual-label studies indicated that testosterone biosynthesis mainly involves the 4-ene pathway (via androstenedione), whereas the formation of epitestosterone uses both the 4-ene and 5-ene (via 5-androstene-3 beta, 17 alpha-diol) pathways. The function of alternative pathways in androgen biosynthesis is discussed, as is the role of temperature in the intratesticular regulation of androgen production.