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1.
J Nat Prod ; 87(4): 906-913, 2024 Apr 26.
Artigo em Inglês | MEDLINE | ID: mdl-38430199

RESUMO

The chemical diversity of annelids, particularly those belonging to the class Sipuncula, remains largely unexplored. However, as part of a Marine Biodiscovery program in Ireland, the peanut worm Phascolosoma granulatum emerged as a promising source of unique metabolites. The purification of the MeOH/CH2Cl2 extract of this species led to the isolation of six new linear guanidine amides, named phascolosomines A-F (1-6). NMR analysis allowed for the elucidation of their structures, all of which feature a terminal guanidine, central amide linkage, and a terminal isobutyl group. Notably, these guanidine amides were present in unusually high concentrations, comprising ∼3% of the dry mass of the organism. The primary concentration of the phascolosomines in the viscera is similar to that previously identified in linear amides from sipunculid worms and marine fireworms. The compounds from sipunculid worms have been hypothesized to be toxins, while those from fireworms are reported to be defensive irritants. However, screening of the newly isolated compounds for inhibitory bioactivity showed no significant inhibition in any of the assays conducted.


Assuntos
Amidas , Anelídeos , Guanidinas , Animais , Amidas/química , Amidas/farmacologia , Amidas/isolamento & purificação , Guanidina/química , Guanidina/farmacologia , Guanidinas/química , Guanidinas/farmacologia , Guanidinas/isolamento & purificação , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Anelídeos/química
2.
Zoology (Jena) ; 144: 125851, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-33227649

RESUMO

Amphinomid fireworms are notorious for their stinging dorsal bristles (notochaetae), but it is still unclear whether the irritation they cause is merely mechanical or if the notochaetae contain toxins. Furthermore, although fireworm chaetae have always been described as calcareous, their composition has never been investigated to date and strong debates are ongoing on their internal structure. Unravelling the native ultrastructure and composition of fireworm chaetae is the first crucial step to assess whether the hypothesis of toxin vehiculation could be fully considered. We examined for the first time the chemical and mineralogical composition, the ultrastructure and the external structure of the dorsal and ventral chaetae of the large species Hermodice carunculata. All the measurements were carried out on samples prepared without the use of chemical reagents, except for those targeted to investigate if decalcification altered the ultrastructure of the chaetae. A crystal-chemical strategy, combining chemical, diffraction and thermal analyses clearly showed the occurrence of crystalline calcium carbonate and clusters of phosphatic amorphous material. Scanning electron micrographs and energy dispersive X-ray measurements showed that the dorsal chaetae have an extremely shallow insertion point in the body respect to the ventral chaetae, that could facilitate the release of the notochaetae in the environment. Their proximal part is characterized by canals with a hexagonal pattern rich in Ca and P, followed by a large cavity upwards. The harpoon-shaped ends and the central canals of the notochaetae completely disappeared after exposure to EDTA. The notochaetae are hollow and may be able to vehicle toxins. The absence of the honeycomb pattern in the distal part of the notochaetae and their slenderness probably contribute to their brittleness and high sensitivity to breakage on contact. These observations constitute keystone understandings to shed light on fireworm defensive and offensive capacities and their ecological success.


Assuntos
Estruturas Animais/química , Estruturas Animais/ultraestrutura , Anelídeos/química , Anelídeos/ultraestrutura , Animais , Microscopia Eletrônica de Varredura
3.
Genome Biol Evol ; 12(10): 1719-1733, 2020 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-32597988

RESUMO

Animals depend on the sequential oxidation of organic molecules to survive; thus, oxygen-carrying/transporting proteins play a fundamental role in aerobic metabolism. Globins are the most common and widespread group of respiratory proteins. They can be divided into three types: circulating intracellular, noncirculating intracellular, and extracellular, all of which have been reported in annelids. The diversity of oxygen transport proteins has been underestimated across metazoans. We probed 250 annelid transcriptomes in search of globin diversity in order to elucidate the evolutionary history of this gene family within this phylum. We report two new globin types in annelids, namely androglobins and cytoglobins. Although cytoglobins and myoglobins from vertebrates and from invertebrates are referred to by the same name, our data show they are not genuine orthologs. Our phylogenetic analyses show that extracellular globins from annelids are more closely related to extracellular globins from other metazoans than to the intracellular globins of annelids. Broadly, our findings indicate that multiple gene duplication and neo-functionalization events shaped the evolutionary history of the globin family.


Assuntos
Anelídeos/genética , Evolução Molecular , Globinas/genética , Família Multigênica , Sequência de Aminoácidos , Animais , Anelídeos/química , Duplicação Gênica , Globinas/química , Filogenia
4.
Dokl Biol Sci ; 490(1): 25-27, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32342322

RESUMO

Here we report the first finding of a frenulate pogonophoran (Annelida, Siboglinidae) in the southern part of the Kara Sea. This finding was made in the Yenisei Gulf in the region of the highest methane concentrations, resulting from the degradation of permafrost under the influence of river flow. It has been suggested that pogonophorans are indicators of hydrocarbon manifestations of various genesis.


Assuntos
Anelídeos/anatomia & histologia , Metano/análise , Animais , Anelídeos/química , Anelídeos/fisiologia , Sedimentos Geológicos , Oceanos e Mares , Federação Russa , Água do Mar/química
5.
Subcell Biochem ; 94: 1-34, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32189294

RESUMO

The coelomic cavity is part of the main body plan of annelids. This fluid filled space takes up a considerable volume of the body and serves as an important site of exchange of both metabolites and proteins. In addition to low molecular substances such as amino acids and glucose and lactate, the coelomic fluid contains different proteins that can arise through release from adjacent tissues (intestine) or from secretion by coelomic cells. In this chapter, we will review the current knowledge about the proteins in the annelid coelomic fluid. Given the number of more than 20,000 extant annelid species, existing studies are confined to a relatively few species. Most studies on the oligochaetes are confined to the earthworms-clearly because of their important role in soil biology. In the polychaetes (which might represent a paraphyletic group) on the other hand, studies have focused on a few species of the Nereidid family. The proteins present in the coelomic fluid serve different functions and these have been studied in different taxonomic groups. In oligochaetes, proteins involved antibacterial defense such as lysenin and fetidin have received much attention in past and ongoing studies. In polychaetes, in contrast, proteins involved in vitellogenesis and reproduction, and the vitellogenic function of coelomic cells have been investigated in more detail. The metal binding metallothioneins as well as antimicrobial peptides, have been investigated in both oligochaetes and polychaetes. In the light of the literature available, this review will focus on lipoproteins, especially vitellogenin, and proteins involved in defense reactions. Other annelid groups such as the Pogonophora, Echiura, and Sipuncula (now considered polychaetes), have not received much attention and therefore, this overview is far from being complete.


Assuntos
Anelídeos/anatomia & histologia , Anelídeos/química , Líquidos Corporais/química , Proteínas/metabolismo , Animais , Anelídeos/classificação , Oligoquetos/anatomia & histologia , Oligoquetos/química , Oligoquetos/metabolismo , Proteínas/classificação
6.
J Nat Med ; 74(1): 275-281, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31493218

RESUMO

We evaluated whether the origins of crude drugs derived from arthropods and annelids could be identified using molecular biological techniques. DNA was extracted from 20 crude drugs prepared from different animals using a commercial kit with added phenol treatment. The target regions used to identify origin were the mitochondrial 16S ribosomal RNA (rRNA), 12S rRNA, and cytochrome oxidase subunit I (COI) gene regions. Extracted DNA was amplified by polymerase chain reaction, and then sequenced by the Sanger method. The aligned sequences were compared with all available sequences using BLAST to estimate the origins of the crude drugs. The origin of crude drugs used in this study could be estimated using this method. The COI region was the best for identifying origin among three regions examined, based on the success rate of PCR amplification and analysis. Moreover, the 12S rRNA region was also useful for origin identification, with the exception of the earthworm. However, the origin of some crude drugs could not be strictly identified due to matches to various species in all three regions. One likely cause was that the species of origin of a crude drug has not been registered in DNA databases. We found that even the same crude drug from the same pharmaceutical company had different origins by production lot or import source country. Therefore, this method is useful not only for DNA-based origin identification but also quality control of production lots.


Assuntos
Anelídeos/química , Artrópodes/química , Extratos Celulares/química , Complexo IV da Cadeia de Transporte de Elétrons/genética , RNA Ribossômico 16S/genética , RNA Ribossômico/genética , Animais , Sequência de Bases , Extratos Celulares/análise , DNA/genética , Filogenia , Reação em Cadeia da Polimerase , Alinhamento de Sequência , Análise de Sequência de DNA
7.
Luminescence ; 34(6): 602-606, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31083817

RESUMO

Terebelliformia is a benthic group of marine annelid worms. The bioluminescence of several species has been reported in taxonomical and histological literature, but very little information is known about the biochemical aspects of this phenomenon. In this study, we examined the basic properties of the luminescence system using an extract of the Japanese terebelliform worm, Thelepus japonicus. The bioluminescence extract was soluble in water, and emitted blue-green light at λmax 508 nm following the addition of divalent cations. This triggering action was highly specific to Fe2+ and addition of ATP, H2 O2 or coelenterazine did not enhance activity. The bioluminescence was inactivated by heat treatment and organic solvents, indicating the involvement of a protein component. These results suggested that Thelepus worm produces light using a novel system that differs from that in other known luminescent annelids.


Assuntos
Anelídeos/química , Trifosfato de Adenosina/química , Animais , Ferro/química , Luminescência , Medições Luminescentes
8.
Biochem J ; 474(24): 4193-4206, 2017 12 11.
Artigo em Inglês | MEDLINE | ID: mdl-29127253

RESUMO

Ferritin, a multimeric cage-like enzyme, is integral to iron metabolism across all phyla through the sequestration and storage of iron through efficient ferroxidase activity. While ferritin sequences from ∼900 species have been identified, crystal structures from only 50 species have been reported, the majority from bacterial origin. We recently isolated a secreted ferritin from the marine invertebrate Chaetopterus sp. (parchment tube worm), which resides in muddy coastal seafloors. Here, we present the first ferritin from a marine invertebrate to be crystallized and its biochemical characterization. The initial ferroxidase reaction rate of recombinant Chaetopterus ferritin (ChF) is 8-fold faster than that of recombinant human heavy-chain ferritin (HuHF). To our knowledge, this protein exhibits the fastest catalytic performance ever described for a ferritin variant. In addition to the high-velocity ferroxidase activity, ChF is unique in that it is secreted by Chaetopterus in a bioluminescent mucus. Previous work has linked the availability of Fe2+ to this long-lived bioluminescence, suggesting a potential function for the secreted ferritin. Comparative biochemical analyses indicated that both ChF and HuHF showed similar behavior toward changes in pH, temperature, and salt concentration. Comparison of their crystal structures shows no significant differences in the catalytic sites. Notable differences were found in the residues that line both 3-fold and 4-fold pores, potentially leading to increased flexibility, reduced steric hindrance, or a more efficient pathway for Fe2+ transportation to the ferroxidase site. These suggested residues could contribute to the understanding of iron translocation through the ferritin shell to the ferroxidase site.


Assuntos
Anelídeos/metabolismo , Ferritinas/química , Animais , Anelídeos/química , Anelídeos/genética , Domínio Catalítico , Cristalografia , Ferritinas/genética , Ferritinas/metabolismo , Humanos , Ferro/metabolismo , Cinética , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Água do Mar/parasitologia
9.
Integr Comp Biol ; 57(1): 18-32, 2017 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-28582579

RESUMO

Bioluminescence, the ability to produce light by living organisms, has evolved independently in numerous lineages across the tree of life. Luminous forms are found in a wide range of taxonomic groups from bacteria to vertebrates, although the great majority of bioluminescent organisms are marine taxa. Within the phylum Annelida, bioluminescence is widespread, present in at least 98 terrestrial and marine species that represent 45 genera distributed in thirteen lineages of clitellates and polychaetes. The ecological diversity of luminous annelids is unparalleled, with species occupying a great variety of habitats including both terrestrial and marine ecosystems, from coastal waters to the deep-sea, in benthic and pelagic habitats from polar to tropical regions. This great taxonomic and ecological diversity is matched by the wide array of bioluminescent colors-including yellow light, which is very rare among marine taxa-different emission wavelengths even between species of the same genus, and varying patterns, chemical reactions and kinetics. This diversity of bioluminescence colors and patterns suggests that light production in annelids might be involved in a variety of different functions, including defensive mechanisms like sacrificial lures or aposematic signals, and intraspecific communication systems. In this review, we explore the world of luminous annelids, particularly focusing on the current knowledge regarding their taxonomic and ecological diversity and discussing the putative functions and chemistries of their bioluminescent systems.


Assuntos
Anelídeos/fisiologia , Luminescência , Animais , Anelídeos/química , Biodiversidade , Ecossistema , Luz
10.
Environ Sci Pollut Res Int ; 24(4): 3759-3768, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-27889887

RESUMO

The present work analyzed three hard-bottom and two soft-bottom species of sabellid polychaetes to determine the content of several heavy metals in their branchial crown and body. The highest concentrations of heavy metals were recorded in the hard-bottom species Branchiomma bairdi, a recent Mediterranean introduction. Differences in the metal concentrations were most notable in the high trace metal levels of the branchial crown for all the studied species. Statistical analysis showed that the Mediterranean hard-bottom species were similar each other in their heavy metal content in the body as well as in the branchial crown and appeared separated from all the other species. Arsenic and vanadium hyperaccumulation in the branchial crowns of the considered sabellid species probably acts as a deterrent for predation. The observed differences among the examined species were discussed not only at the light of habitat colonization but also in terms of the phylogeny.


Assuntos
Anelídeos/química , Metais Pesados/análise , Poliquetos/química , Animais , Arsênio/análise , Ecossistema
11.
Int Braz J Urol ; 42(4): 825-37, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27564297

RESUMO

INTRODUCTION: We investigate the effect of active peptide from Urechis unicinctus (UU) by high temperature/pressure and ultra-wave assisted lysis on erectile dysfunction in streptozotocin-induced diabetic rats. MATERIALS AND METHODS: Forty 12-week-old Sprague-Dawley rats were used in this study. Diabetes was induced by a one-time intraperitoneal injection of streptozotocin (50mg/kg). One week later, the diabetic rats were randomly divided into four groups: normal control, untreated diabetes control, and groups treated with 100 or 500mg/kg/d UU peptide. Rats were fed with UU peptide by intragastric administration for 8 weeks. After 8 weeks, penile hemodynamic function was evaluated in all groups by measuring the intracavernosal pressure after electrostimulating the cavernous nerve. Nitric oxide (NO) and cyclic guanosine monophosphate (cGMP) activities were measured and endothelial nitric oxide synthase (eNOS) and neuronal NOS (nNOS) protein expression. was determined by Western blot. RESULTS: Maximum intracavernosal pressure in diabetic control rats decreased significantly compared to normal control rats, and was increased significantly compared to untreated diabetic rats after UU peptide supplementation. Treatment with the higher dose of UU peptide significantly increased the NO and cGMP levels compared with the diabetic control group. Decreased activity and expression eNOS and nNOS were found in the diabetic rats compared with the normal control group. Decreased eNOS and nNOS in diabetic rats were improved by UU peptide administration. CONCLUSIONS: Active peptide from UU ameliorates erectile function in a streptozotocin induced diabetic rat model of erectile dysfunction.


Assuntos
Anelídeos/química , Diabetes Mellitus Experimental/complicações , Disfunção Erétil/tratamento farmacológico , Peptídeos/farmacologia , Animais , Células Cultivadas , Diabetes Mellitus Experimental/induzido quimicamente , Disfunção Erétil/etiologia , Disfunção Erétil/fisiopatologia , Masculino , Pênis/efeitos dos fármacos , Peptídeos/análise , Peptídeos/uso terapêutico , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Estreptozocina , Temperatura
12.
Int. braz. j. urol ; 42(4): 825-837, July-Aug. 2016. tab, graf
Artigo em Inglês | LILACS | ID: lil-794686

RESUMO

ABSTRACT Introduction: We investigate the effect of active peptide from Urechis unicinctus (UU) by high temperature/pressure and ultra-wave assisted lysis on erectile dysfunction in streptozotocin-induced diabetic rats. Materials and Methods: Forty 12-week-old Sprague-Dawley rats were used in this study. Diabetes was induced by a one-time intraperitoneal injection of streptozotocin (50mg/kg). One week later, the diabetic rats were randomly divided into four groups: normal control, untreated diabetes control, and groups treated with 100 or 500mg/kg/d UU peptide. Rats were fed with UU peptide by intragastric administration for 8 weeks. After 8 weeks, penile hemodynamic function was evaluated in all groups by measuring the intracavernosal pressure after electrostimulating the cavernous nerve. Nitric oxide (NO) and cyclic guanosine monophosphate (cGMP) activities were measured and endothelial nitric oxide synthase (eNOS) and neuronal NOS (nNOS) protein expression was determined by Western blot. Results: Maximum intracavernosal pressure in diabetic control rats decreased significantly compared to normal control rats, and was increased significantly compared to untreated diabetic rats after UU peptide supplementation. Treatment with the higher dose of UU peptide significantly increased the NO and cGMP levels compared with the diabetic control group. Decreased activity and expression eNOS and nNOS were found in the diabetic rats compared with the normal control group. Decreased eNOS and nNOS in diabetic rats were improved by UU peptide administration. Conclusions: Active peptide from UU ameliorates erectile function in a streptozotocin induced diabetic rat model of erectile dysfunction.


Assuntos
Animais , Masculino , Ratos , Peptídeos/farmacologia , Diabetes Mellitus Experimental/complicações , Disfunção Erétil/tratamento farmacológico , Anelídeos/química , Pênis/efeitos dos fármacos , Peptídeos/análise , Peptídeos/uso terapêutico , Temperatura , Distribuição Aleatória , Células Cultivadas , Ratos Sprague-Dawley , Estreptozocina , Diabetes Mellitus Experimental/induzido quimicamente , Disfunção Erétil/etiologia , Disfunção Erétil/fisiopatologia
13.
Aquat Toxicol ; 176: 88-96, 2016 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-27126443

RESUMO

Understanding the role of species traits in chemical exposure is crucial for bioaccumulation and toxicity assessment of chemicals. We measured and modelled bioaccumulation of polychlorinated biphenyls (PCBs) in Chironomus riparius, Hyalella azteca, Lumbriculus variegatus and Sphaerium corneum. We used a battery test procedure with multiple enclosures in one aquarium, which maximized uniformity of exposure for the different species, such that the remaining variability was due mostly to species traits. The relative importance of uptake from either pore water or sediment ingestion was manipulated by using 28 d aged standard OECD sediment with low (1%) and medium (5%) OM content and 13 months aged sediment with medium OM (5%) content. Survival was ≥76% and wet weight increased for all species. Reproduction of H. azteca and weight gain of H. azteca and S. corneum were significantly higher in the medium OM aged sediments than in other sediments, perhaps due to a more developed microbial community (i.e., increase in food resources). Biota-sediment accumulation factors (BSAF) ranged from 3 to 114, depending on species and PCB congener, with C. riparius (3-10)

Assuntos
Invertebrados/efeitos dos fármacos , Bifenilos Policlorados/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Anelídeos/química , Anelídeos/efeitos dos fármacos , Anelídeos/metabolismo , Artrópodes/química , Artrópodes/efeitos dos fármacos , Artrópodes/metabolismo , Crustáceos/química , Crustáceos/efeitos dos fármacos , Crustáceos/metabolismo , Sedimentos Geológicos/química , Invertebrados/química , Invertebrados/metabolismo , Modelos Teóricos , Óvulo/efeitos dos fármacos , Óvulo/crescimento & desenvolvimento , Óvulo/metabolismo , Bifenilos Policlorados/química , Poluentes Químicos da Água/química
14.
Chem Biol Drug Des ; 88(1): 129-41, 2016 07.
Artigo em Inglês | MEDLINE | ID: mdl-26850929

RESUMO

HirudinP6 is a glycosylated and sulfated high affinity thrombin inhibitory protein isolated from Hirudineria manillensis. In this study, designing of novel bivalent thrombin inhibitory peptides based on this hirudin isoform is described. The structural and functional impact of varying linker length and glycosylation on their inhibitory potencies and binding kinetics were assessed. The bivalent peptides were obtained by tethering an active site blocking fPRP motif with the carboxy terminal 22 residue segment of hirudin P6 (HP642-63 ) by varying number of glycine residues in the linker region. Among them, analog BiG1 -HP6 inhibited thrombin with a Ki of 5.12 nm which was comparable to that of glycosylated (disaccharide bearing) and non-sulfated full length hirudin P6 protein (Ki = 6.38 nm). Binding kinetics studies revealed increasing linker length can decrease the association rates of peptide─thrombin interactions. Similarly, glycosylation was found to negatively modulate the inhibitory potencies of these peptides by decreasing their rates of association with thrombin. Molecular docking studies revealed that increasing linker length can compromise the electrostatic interactions with the prime subsite residues of thrombin and provided structural explanation for the observed effect of linker length on association rates. These findings thus enhance our understanding of thrombin─(glyco)peptide interactions and provide key insights into the designing of efficient thrombin inhibitors and allosteric modulators of therapeutic potential.


Assuntos
Antitrombinas/farmacologia , Desenho de Fármacos , Glicopeptídeos/farmacologia , Hirudinas/farmacologia , Modelos Moleculares , Fragmentos de Peptídeos/farmacologia , Trombina/antagonistas & inibidores , Regulação Alostérica , Animais , Anelídeos/química , Antitrombinas/química , Antitrombinas/metabolismo , Domínio Catalítico , Glicopeptídeos/química , Glicopeptídeos/metabolismo , Glicosilação , Hirudinas/química , Hirudinas/metabolismo , Humanos , Cinética , Ligantes , Simulação de Acoplamento Molecular , Peso Molecular , Fragmentos de Peptídeos/química , Fragmentos de Peptídeos/metabolismo , Conformação Proteica , Domínios e Motivos de Interação entre Proteínas , Eletricidade Estática , Ressonância de Plasmônio de Superfície , Treonina/química , Treonina/metabolismo , Trombina/química , Trombina/metabolismo
15.
Adv Healthc Mater ; 5(7): 795-801, 2016 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-26806763

RESUMO

Transcatheter embolization is used to treat vascular malformations and defects, to control bleeding, and to selectively block blood supply to tissues. Liquid embolics are used for small vessel embolization that require distal penetration. Current liquid embolic agents have serious drawbacks, mostly centered around poor handling characteristics and toxicity. In this work, a water-borne in situ setting liquid embolic agent is described that is based on electrostatically condensed, oppositely charged polyelectrolytes-complex coacervates. At high ionic strengths, the embolic coacervates are injectable fluids that can be delivered through long narrow microcatheters. At physiological ionic strength, the embolic coacervates transition into a nonflowing solid morphology. Transcatheter embolization of rabbit renal arteries demonstrated capillary level penetration, homogeneous occlusion, and 100% devascularization of the kidney, without the embolic crossing into venous circulation. The benign water-borne composition and setting mechanism avoids many of the problems of current liquid embolics, and provides precise temporal and spatial control during endovascular embolization.


Assuntos
Adesivos/farmacologia , Anelídeos/química , Organismos Aquáticos/química , Embolização Terapêutica , Procedimentos Endovasculares , Oceanos e Mares , Água/química , Animais , Injeções , Concentração Osmolar , Pressão , Coelhos , Artéria Renal/efeitos dos fármacos , Artéria Renal/patologia , Reologia , Viscosidade
16.
J Med Food ; 18(2): 190-4, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25289478

RESUMO

To further utilize glycosaminoglycan from Urechis unicinctus, the hypoglycemic effect and possible mechanism of glycosaminoglycan on diabetic mice were evaluated. Diabetes was induced in mice by intraperitoneal injections of streptozotocin for 3 consecutive days and fed with high-sugar and high-lipid fodder. After diabetes was confirmed, the hypoglycemic effect of glycosaminoglycan from U. unicinctus was investigated in the diabetic mice. Results demonstrated that glycosaminoglycan could significantly decrease blood glucose concentrations, HOMA-IR, AUG, and liver MDA content in diabetic mice. In addition, it significantly enhanced liver SOD and GSH-Px activity, as well as liver GCK activity and hepatic glycogen levels. Glycosaminoglycan from U. unicinctus exhibited efficacy against diabetes, suggesting its potential use as a natural intervention against diabetes.


Assuntos
Anelídeos/química , Diabetes Mellitus Experimental/tratamento farmacológico , Glicosaminoglicanos/farmacologia , Hipoglicemiantes/farmacologia , Animais , Glicemia/efeitos dos fármacos , Diabetes Mellitus Experimental/induzido quimicamente , Glucoquinase/metabolismo , Glutationa Peroxidase/metabolismo , Insulina/sangue , Glicogênio Hepático/metabolismo , Malondialdeído/sangue , Camundongos , Estreptozocina , Superóxido Dismutase/metabolismo
17.
Environ Res ; 133: 371-9, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-24952460

RESUMO

BACKGROUND: Feminization of animals derived from areas polluted by endocrine disrupting chemicals (EDCs) has been observed in all classes of vertebrates. However, feminization of artificially reared offspring by feeding of specific living organisms has never been reported. METHODS: Different food (including Limnodilus spp collected from the wild) and time treatment were applied to southern catfish. In addition, EDCs in Limnodilus spp., an annelid worm collected from wild contaminated small streams, was detected by LC-MS (Liquid chromatography-mass spectrometry). Serum estradiol-17ß and vitellogenin (VTG) levels and gonadal Sf1, Dmrt1, Foxl2, Cyp19a1a expression levels in the catfish were measured through Estradiol/VTG EIA Kit and real-time PCR. RESULTS: Here we report that feeding of Limnodilus spp. resulted in complete feminization of southern catfish, which has a 1:1 sex ratio in wild conditions. Furthermore, HPLC analysis showed that the extraction of Limnodilus spp. contained EDCs, including bisphenol A (BPA), diethylstilbestrol (DES), 4-tert-octylphenol (4-t-OP) and 4-nonylphenol (4-NP), which were further confirmed by LC-MS. Feeding southern catfish using commercial diets sprayed with EDCs cocktail also resulted in 100% female, whereas the control fish displayed approximate 1:1 sex ratio. Limnodilus spp. fed fish displayed similar serum estradiol-17ß and VTG levels and gonadal Sf1, Dmrt1, Foxl2, Cyp19a1a expression levels to those of female control. CONCLUSION: These results demonstrated that EDCs in Limnodilus spp. cause southern catfish feminization by affecting aromatase expression and endogenous estrogen level. This is the first report showing that feeding of any living organism resulted in complete feminization of a vertebrate.


Assuntos
Anelídeos/química , Peixes-Gato , Disruptores Endócrinos/análise , Feminização/induzido quimicamente , Poluição Química da Água/efeitos adversos , Animais , Cromatografia Líquida , Disruptores Endócrinos/efeitos adversos , Feminino , Gônadas/metabolismo , Gônadas/patologia , Masculino , Espectrometria de Massas , Diferenciação Sexual , Vitelogeninas/sangue
18.
J Agric Food Chem ; 61(21): 4997-5002, 2013 May 29.
Artigo em Inglês | MEDLINE | ID: mdl-23635317

RESUMO

Knowledge about the enantioselective bioavailability of chiral pesticides in aquatic organisms facilitates more accurate interpretation of their environmental behaviors. In this study, the enantioselective bioaccumulation of metalaxyl enantiomers in Tubifex tubifex was detected in two uptake pathways. For the spike water treatment, a 16 day exposure experiment was employed and the enantiomer fractions (EFs) in tubifex tissue were maintained approximately at 0.47 during the experiment. For the spike sediment treatment, a 14 day bioaccumulation period indicated the concentrations of (-)-(R)-metalaxyl were higher than those of (+)-(S)-metalaxyl. Therefore, the bioaccumulation of metalaxyl in worms was enantioselective for these treatments. With the presence of tubifex, higher concentrations of metalaxyl in overlying water and lower concentrations in sediment were detected than in worm-free treatments. This means that tubifex has positive functions in metalaxyl's diffusion from the sediment to overlying water and in the degradation of the sediment-associated metalaxyl.


Assuntos
Alanina/análogos & derivados , Anelídeos/metabolismo , Fungicidas Industriais/metabolismo , Sedimentos Geológicos/química , Alanina/química , Alanina/metabolismo , Animais , Anelídeos/química , Fungicidas Industriais/química , Cinética , Estereoisomerismo , Poluentes Químicos da Água/química , Poluentes Químicos da Água/metabolismo
19.
Int J Biol Macromol ; 49(4): 523-6, 2011 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-21723315

RESUMO

A water-soluble polysaccharide, named as SNP, was extracted and fractioned from the body wall of Sipunculus nudus L. by DEAE-Sepharose anion exchange and Sepharose CL-6B column chromatography. The evaluation for anti-hypoxia activity demonstrated that SNP had significant anti-hypoxic activity on normobarie hypoxia, chemical intoxicant hypoxia and acute cerebral ischemia hypoxia models in mice. SNP also enhanced the number of red blood cell count (RBC) and the concentration of hemoglobin (HGB). The structural characteristics of SNP investigated by high performance size exclusion chromatography, Fourier transform infrared spectroscopy and gas chromatography-mass spectrometry indicated that SNP was a homogeneous polysaccharide with a molecular mass of 350 kD and was composed of rhamnose (28%), fucose (16%) and galactose (56%). The results suggested that SNP could be explored as a novel potential anti-hypoxia agent.


Assuntos
Anelídeos/química , Hipóxia/tratamento farmacológico , Polissacarídeos/isolamento & purificação , Polissacarídeos/uso terapêutico , Animais , Isquemia Encefálica/sangue , Metilação/efeitos dos fármacos , Camundongos , Polissacarídeos/farmacologia , Nitrito de Sódio/toxicidade , Espectrofotometria Infravermelho , Análise de Sobrevida , Testes de Toxicidade
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