RESUMO
In this study, the dissolution of polysaccharides into an ionic liquid was investigated and applied as a coating onto the capillary walls of a fused-silica capillary in open-tubular CEC. The coating was evaluated by examining the chiral separation of two analytes (thiopental, sotalol) with three cellulose derivatives (cellulose acetate, cellulose acetate phthalate, and cellulose acetate butyrate). Baseline separation of thiopental enantiomers was achieved by use of each polysaccharide coating (Rs: 7.0, 8.1, 7.1), while sotalol provided partial resolution (Rs: 0.7, 1.0, 0.9). In addition, reproducibility of the cellulose-coated capillaries was evaluated by estimating the run-to-run and capillary-to-capillary RSD values of the EOF. Both stability and reproducibility were very good with RSD values of less than 7%.
Assuntos
Eletrocromatografia Capilar/instrumentação , Celulose/análogos & derivados , Imidazóis/química , Líquidos Iônicos/química , Antagonistas Adrenérgicos beta/isolamento & purificação , Anestésicos Intravenosos/isolamento & purificação , Eletrocromatografia Capilar/economia , Celulose/química , Dióxido de Silício/química , Sotalol/isolamento & purificação , Estereoisomerismo , Tiopental/isolamento & purificaçãoAssuntos
Anestésicos Inalatórios/química , Anestésicos Intravenosos/química , Medicamentos Genéricos/química , Éteres Metílicos/química , Propofol/química , Anestésicos Inalatórios/síntese química , Anestésicos Inalatórios/isolamento & purificação , Anestésicos Intravenosos/síntese química , Anestésicos Intravenosos/isolamento & purificação , Química Farmacêutica , Contaminação de Medicamentos , Indústria Farmacêutica , Embalagem de Medicamentos , Estabilidade de Medicamentos , Medicamentos Genéricos/isolamento & purificação , Excipientes , Éteres Metílicos/síntese química , Éteres Metílicos/isolamento & purificação , Propofol/isolamento & purificação , SevofluranoAssuntos
Analgésicos Opioides/isolamento & purificação , Anestésicos Intravenosos/isolamento & purificação , Sufentanil/isolamento & purificação , Analgésicos Opioides/sangue , Anestésicos Intravenosos/sangue , Cuidados Críticos , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Sufentanil/sangueRESUMO
Concentrations in breast milk of medications used during general anesthesia were measured to determine whether interruption of breast-feeding was indicated. Breast milk and maternal blood samples were obtained from nine women undergoing tubal sterilization under general anesthesia. Concentrations of methohexital, meperidine, diazepam, and nordiazepam were determined for each sample by gas chromatography. Methohexital levels declined rapidly after the first hour and were undetectable at 24 hours. Meperidine was present in both milk and blood during the recovery period but not at 24 hours. Infant-exposure indices for methohexital were less than 1% and ranged from 1.2% to 3.5% for meperidine. The maximum doses of methohexital and meperidine to an infant, in a 100 mL feeding 1 hour after induction of anesthesia were estimated to be 0.04 mg and 0.06 mg, respectively. Diazepam and nordiazepam were not detectable in any sample of milk or blood. The maximum possible infant-exposure index for diazepam would be 3%. The amounts of methohexital, meperidine and diazepam excreted into breast milk do not warrant interruption of breast-feeding.