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2.
Am J Clin Pathol ; 144(2): 305-14, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26185316

RESUMO

OBJECTIVES: Abnormalities of the RUNX1 gene in childhood B-acute lymphoblastic leukemia (B-ALL) are manifested by ETV6-RUNX1 or RUNX1 amplification. A detailed comparison between the two regarding clinicopathologic features with genetic analysis has not been performed previously. This parallel study assessed how different RUNX1 abnormalities affect the clinicopathology of B-ALL. METHODS: We compared clinicopathologic factors, including age, sex, WBC count, cerebrospinal fluid (CSF) involvement, immunophenotype, and blast proliferation rate between B-ALL with RUNX1 amplification (10 cases) and B-ALL with ETV6-RUNX1 translocation (67 cases) in childhood B-ALL. RESULTS: CD7 was often expressed in RUNX1 amplification but not in ETV6-RUNX1 (44% vs 0%, P = .0001) and appeared to correlate with CSF involvement in the former group (3/4 [75%]). CD13 was often detected in ETV6-RUNX1 with additional RUNX1 gain (38%) with an even higher frequency in double ETV6-RUNX1 translocation (77%), but was not detected in RUNX1 amplification (0%, P < .05). Children with RUNX1 amplification were older and more often CSF positive, while those with ETV6-RUNX1 were younger, more frequently had hyperleukocytosis, and had higher blast proliferation rates. CONCLUSIONS: RUNX1 copy numbers seem to be proportional to the age of B-ALL onset and the frequency of CSF involvement, while RUNX1 amplification vs translocation causes aberrant expression of CD7 and CD13, respectively.


Assuntos
Subunidade alfa 2 de Fator de Ligação ao Core/genética , Leucemia-Linfoma Linfoblástico de Células Precursoras/genética , Leucemia-Linfoma Linfoblástico de Células Precursoras/patologia , Adolescente , Idade de Início , Animais , Antígenos CD7/biossíntese , Antígenos CD7/imunologia , Antígenos CD13/biossíntese , Antígenos CD13/imunologia , Pré-Escolar , Feminino , Citometria de Fluxo , Amplificação de Genes , Humanos , Imunofenotipagem , Hibridização in Situ Fluorescente , Masculino , Leucemia-Linfoma Linfoblástico de Células Precursoras/líquido cefalorraquidiano , Coelhos , Translocação Genética , Adulto Jovem
3.
Clin Cancer Res ; 20(11): 2851-61, 2014 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-24727323

RESUMO

PURPOSE: Cell adhesion molecule 1 (CADM1), initially identified as a tumor suppressor gene, has recently been reported to be ectopically expressed in primary adult T-cell leukemia-lymphoma (ATL) cells. We incorporated CADM1 into flow-cytometric analysis to reveal oncogenic mechanisms in human T-cell lymphotrophic virus type I (HTLV-I) infection by purifying cells from the intermediate stages of ATL development. EXPERIMENTAL DESIGN: We isolated CADM1- and CD7-expressing peripheral blood mononuclear cells of asymptomatic carriers and ATLs using multicolor flow cytometry. Fluorescence-activated cell sorted (FACS) subpopulations were subjected to clonal expansion and gene expression analysis. RESULTS: HTLV-I-infected cells were efficiently enriched in CADM1(+) subpopulations (D, CADM1(pos)CD7(dim) and N, CADM1(pos)CD7(neg)). Clonally expanding cells were detected exclusively in these subpopulations in asymptomatic carriers with high proviral load, suggesting that the appearance of D and N could be a surrogate marker of progression from asymptomatic carrier to early ATL. Further disease progression was accompanied by an increase in N with a reciprocal decrease in D, indicating clonal evolution from D to N. The gene expression profiles of D and N in asymptomatic carriers showed similarities to those of indolent ATLs, suggesting that these subpopulations represent premalignant cells. This is further supported by the molecular hallmarks of ATL, that is, drastic downregulation of miR-31 and upregulation of abnormal Helios transcripts. CONCLUSION: The CADM1 versus CD7 plot accurately reflects disease progression in HTLV-I infection, and CADM1(+) cells with downregulated CD7 in asymptomatic carriers have common properties with those in indolent ATLs.


Assuntos
Antígenos CD7/biossíntese , Moléculas de Adesão Celular/biossíntese , Infecções por HTLV-I/metabolismo , Imunoglobulinas/biossíntese , Leucemia-Linfoma de Células T do Adulto/metabolismo , Adulto , Biomarcadores Tumorais/análise , Molécula 1 de Adesão Celular , Progressão da Doença , Regulação para Baixo , Feminino , Citometria de Fluxo , Humanos , Leucemia-Linfoma de Células T do Adulto/patologia , Leucemia-Linfoma de Células T do Adulto/virologia , Masculino , Análise de Sequência com Séries de Oligonucleotídeos , Reação em Cadeia da Polimerase em Tempo Real
4.
Genes Chromosomes Cancer ; 51(4): 328-37, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22162288

RESUMO

ETV6 (TEL) rearrangements are favorable in pediatric acute lymphoblastic leukemia but are less well characterized in myeloid malignancies. We investigated 9,550 patients with myeloid disorders for ETV6 rearrangements by chromosome banding analysis and interphase fluorescence in situ hybridization. ETV6 rearrangements were identified in 51 of 9,550 (0.5%) patients (range, 19.2-85.3 years). Frequencies were in detail: acute myeloid leukemia (AML): 40 of 3,798, 1.1%; myelodysplastic syndromes (MDS): 6 of 3,375, 0.2%; myeloproliferative neoplasms (MPNs): 5 of 1,720, 0.3%; MDS/MPN: 0 of 210; and chronic myelomonocytic leukemia: 0 of 447. Thirty-three different partner bands of ETV6 were identified, and most were recurrent: 3q26 (n = 10), 5q33 (n = 4), 17q11 (n = 3), 22q12 (n = 3), 5q31 (n = 2), and 2q31 (n = 2). Additional chromosomal abnormalities were identified in 29 of 51 (57%) ETV6 rearranged cases. In AML, ETV6 rearrangements were frequently associated with NPM1 (9/39, 23%) and RUNX1 mutations (6/31, 19%). The FAB M0 subtype was more frequent in ETV6 rearranged de novo AML than other AML (P < 0.001); expression of CD7 and CD34 by immunophenotyping was higher in ETV6 rearranged AML compared with other subgroups. Survival of 29 ETV6 rearranged de novo AML was compared with 818 AML from other cytogenetic subgroups. Median overall and event-free survival of ETV6 rearranged cases was similar to the intermediate-risk cohort (26.3 vs. 62.2 months and 14.0 vs. 15.4 months) defined according to Medical Research Council criteria. Our study confirms the variety of ETV6 rearrangements in AML, MDS, and MPNs often in association with other genetic events. Prognosis of ETV6 rearranged de novo AML seems to be intermediate, which should be independently confirmed.


Assuntos
Rearranjo Gênico , Leucemia Mieloide Aguda/genética , Síndromes Mielodisplásicas/genética , Transtornos Mieloproliferativos/genética , Proteínas Proto-Oncogênicas c-ets/genética , Proteínas Repressoras/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Antígenos CD34/biossíntese , Antígenos CD7/biossíntese , Bandeamento Cromossômico , Subunidade alfa 2 de Fator de Ligação ao Core/genética , Intervalo Livre de Doença , Feminino , Humanos , Imunofenotipagem , Hibridização in Situ Fluorescente , Cariótipo , Leucemia Mieloide Aguda/mortalidade , Leucemia Mieloide Aguda/patologia , Masculino , Pessoa de Meia-Idade , Síndromes Mielodisplásicas/mortalidade , Síndromes Mielodisplásicas/patologia , Transtornos Mieloproliferativos/mortalidade , Transtornos Mieloproliferativos/patologia , Proteínas Nucleares/genética , Nucleofosmina , Prognóstico , Adulto Jovem , Variante 6 da Proteína do Fator de Translocação ETS
5.
J Appl Genet ; 50(1): 73-6, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19193987

RESUMO

Pericentric inversion of chromosome 9 involving the qh region is relatively common as a constitutional genetic aberration without any apparent phenotypic consequences. However, it has not been established as an acquired abnormality in cancer. Among the three patients reported so far in the literature with acquired inv(9), only one had acute myeloid leukemia (AML). Here we describe an unique case where both chromosomes 9 presented with an acquired pericentric inversion with breakpoints at 9p13 and 9q12 respectively, in a AML patient with aberrant CD7 and CD9 positivity. Additionally, one der(9) also showed short arm deletion at 9p21 to the centromeric region and including the p16 gene. The constitutional karyotype was normal. This is probably the first report describing an acquired inv(9) involving both chromosomes 9 in AML. The possible significance of this inversion is discussed.


Assuntos
Inversão Cromossômica , Cromossomos Humanos Par 9 , Leucemia Mieloide Aguda/genética , Antígenos CD/biossíntese , Antígenos CD7/biossíntese , Bandeamento Cromossômico , Deleção Cromossômica , Mapeamento Cromossômico , Humanos , Hibridização in Situ Fluorescente , Cariotipagem , Glicoproteínas de Membrana/biossíntese , Tetraspanina 29
6.
Cytometry B Clin Cytom ; 76(3): 169-74, 2009 May.
Artigo em Inglês | MEDLINE | ID: mdl-18956470

RESUMO

BACKGROUND: Diagnosis of Hodgkin lymphoma (HL) is sometimes complicated by the scarcity of neoplastic cells in a reactive inflammatory background. Immunophenotyping by flow cytometry (FC) has not played a significant role in HL diagnosis because of its consistent failure to identify these neoplastic cells. However, HL-infiltrating T cells have been shown to play a role in HL pathogenesis. This study characterizes the FC immunophenotype of these T lymphocytes to determine whether they can be used to assist in the diagnosis of HL. METHODS: Cell suspensions from 76 lymph nodes involved by HL and 156 lymph nodes with reactive lymphadenopathy (LAD) were analyzed by flow cytometry to assess the expression of T-cell antigens. RESULTS: The CD4:CD8 ratio and CD7 expression in both CD4(+) and CD8(+) T cells are increased in HL compared with reactive lymph nodes and there are significant differences between these features in different subtypes of HL. However, only the expression of CD7 in CD4(+) T cells distinguishes between HL and reactive LAD. This is especially true for classical HL in younger patients. Using a CD7 mean fluorescence intensity (MFI) cutoff value generated by this data, 37/47 FNA specimens were correctly diagnosed. CONCLUSIONS: There are significant differences in the immunophenotypes of HL-infiltrating T cells. Of these, the CD7 expression in CD4(+) T cells discriminates between HL and reactive LAD, suggesting that this could be a useful and practical adjunctive tool in the diagnosis of HL. It may also further our understanding of the pathophysiology of this disease.


Assuntos
Antígenos CD7/biossíntese , Linfócitos T CD4-Positivos/metabolismo , Linfócitos T CD8-Positivos/metabolismo , Doença de Hodgkin/metabolismo , Linfócitos do Interstício Tumoral/metabolismo , Subpopulações de Linfócitos T/metabolismo , Adulto , Antígenos CD7/genética , Antígenos CD7/metabolismo , Complexo CD3/biossíntese , Antígenos CD4/biossíntese , Antígenos CD8/biossíntese , Feminino , Citometria de Fluxo , Doença de Hodgkin/diagnóstico , Humanos , Imunofenotipagem , Masculino , Sensibilidade e Especificidade
8.
Eur J Haematol ; 80(2): 151-9, 2008 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-18005390

RESUMO

OBJECTIVE: For transplantation of cord blood (CB) cells, it is important to select a CB sample that can reconstitute not only myelo-erythropoiesis but also lymphopoiesis in recipients. However, until now the reconstitution ability of CB samples has been assessed by colony forming unit-culture (CFU-C) assay or by simply counting CD34+ cells. The present study aims at establishing a method capable of assessing the potential of T lymphopoieses of CB samples. METHODS: CD34+ CD38- cells sorted from CB were cultured on a monolayer of murine stromal cell line TSt-4, transduced with the human Delta-like 1 gene. RESULTS: Immature T cells expressing CD5 and/or CD7 were generated in the culture. As these immature T cells can easily be discriminated from mature T cells that are included in the mononuclear cell population (MNCs), we can use the MNCs as starting material for quantification of progenitors capable of generating T cells (TGP). By applying a limiting dilution analysis, we succeeded in determining the frequency of TGP in MNCs. It was found that the ratios for the number of TGP vs. that of CFU-C differ among CB samples maximally by 3.5 times. CONCLUSION: The present assay system provides a novel tool for the evaluation of CB samples, especially for their T-cell-generating potential.


Assuntos
Transplante de Células-Tronco de Sangue do Cordão Umbilical/métodos , Sangue Fetal/citologia , Células-Tronco/citologia , Linfócitos T/citologia , ADP-Ribosil Ciclase 1/biossíntese , Animais , Antígenos CD34/biossíntese , Antígenos CD7/biossíntese , Antígenos CD5/biossíntese , Proteínas de Ligação ao Cálcio , Citometria de Fluxo , Rearranjo Gênico , Humanos , Peptídeos e Proteínas de Sinalização Intercelular/genética , Leucócitos Mononucleares/citologia , Proteínas de Membrana/genética , Camundongos , Camundongos Endogâmicos C57BL , Células Estromais/metabolismo
9.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 15(2): 421-4, 2007 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-17493361

RESUMO

The purpose of this study was to investigate the prognosis of acute myelogenous leukemia (AML), acute lymphoblastic leukemia (ALL), lymphoid antigen-positive acute myeloid leukemia (Ly + AML), myeloid antigen-positive acute leukemia (My + ALL) and biphenotypic acute leukemia (BAL). Immunophenotyping was performed on medullary specimens of 197 acute leukemia (AL) patients by using three-color flow cytometry analysis and CD45/SSC gating. The scoring systems proposed by EGIL was adopted to classify the AL patients into five groups: 43 of ALL, 53 of AML, 53 of My + ALL, 39 of Ly + AML and 9 of BAL patients. The results showed that in Ly + AML, CD7 was the most common (53.8%) as compared to other lymphoid markers, however, in My + ALL CD13 was the most common (47.2%) as compared to other myeloid markers. Compared with Ly + AML, My + ALL had higher incidences of enlargement of liver, spleen and lymphnodes significantly (P<0.05). As for the case numbers of WBC counts > 100 x 10(9)/L, the positive rate of CD34 and the complete remission rate there was no obvious difference between groups of Ly + AML and My + ALL (P>0.05). As for incidences of enlargement of liver, spleen and lymphnodes, the case numbers of WBC counts > 100 x 10(9)/L, the positive rate of CD34 and complete remission rate, no obvious difference was found between ALL and My + ALL (P>0.05). Compared with AML, Ly + AML had lower complete remission rate significantly (P<0.05). As for incidences of enlargement of liver, spleen and lymphnodes, the case numbers of WBC counts > 100 x 10(9)/L and the positive rate of CD34, no obvious difference was found between AML and Ly + AML (P>0.05). Compared with Ly + AML and My + ALL, BAL showed no significant difference in complete remission rate (P>0.05) because the number of BAL patients was too small. It is concluded that since Ly + AML has lymphoid markers, and the prognosis of Ly + AML is worse than AML, the clinical therapy for Ly + AML should contain both AML and ALL. Though My + ALL had myeloid markers, no significant difference was found between My + ALL and ALL, it might be supposed that their therapy could be the same.


Assuntos
Leucemia Mieloide Aguda/imunologia , Leucemia-Linfoma Linfoblástico de Células Precursoras/imunologia , Adolescente , Adulto , Idoso , Antígenos CD34/biossíntese , Antígenos CD7/biossíntese , Antígenos CD13/biossíntese , Criança , Pré-Escolar , Feminino , Humanos , Imunofenotipagem , Leucemia Mieloide Aguda/classificação , Masculino , Pessoa de Meia-Idade , Leucemia-Linfoma Linfoblástico de Células Precursoras/classificação , Prognóstico
10.
Hum Reprod ; 22(4): 919-26, 2007 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-17208945

RESUMO

BACKGROUND: Uterine lymphoid cell repertoires are specialized in order to meet the twin demands of successful pregnancy and local immunosurveillance. The possibility that some of these populations might differentiate locally from progenitor cells has been proposed. METHODS: Endometrial tissue from women with a history of infertility as well as fertile controls was examined for haematopoietic stem cells (HSCs) and lymphoid progenitors using three-colour flow cytometry. RESULTS: Significant populations of phenotypic HSCs (CD34+ CD45+ ) were detected in all samples, a high proportion of which co-expressed the differentiation marker CD45RA (45.7%), indicating ongoing differentiation. Almost 30% of uterine HSCs co-expressed CD56 and 44% co-expressed CD7, suggesting the presence of lymphoid progenitors. Small proportions expressed CD127 and CD122, receptors for interleukin (IL)-7 and IL-15, respectively. HSC numbers were similar in the endometrial samples from fertile and infertile women. However, the proportion co-expressing the natural killer (NK) antigen CD56 was significantly increased compared with HSCs found in the endometrium of fertile controls (P = 0.002). CONCLUSIONS: This is the first demonstration of cells with an HSC phenotype in the human endometrium, and increased proportions of NK progenitors in endometrium of women with infertility suggests a dysregulation of this pathway that may contribute to infertility.


Assuntos
Endométrio/patologia , Células-Tronco Hematopoéticas/citologia , Infertilidade/imunologia , Adulto , Antígenos CD7/biossíntese , Antígeno CD56/biossíntese , Feminino , Citometria de Fluxo , Células-Tronco Hematopoéticas/patologia , Humanos , Infertilidade/patologia , Interleucina-15/metabolismo , Subunidade beta de Receptor de Interleucina-2/biossíntese , Interleucina-7/metabolismo , Subunidade alfa de Receptor de Interleucina-7/biossíntese , Pessoa de Meia-Idade , Fenótipo
11.
Leuk Res ; 31(2): 157-62, 2007 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-16837044

RESUMO

AML patients with normal karyotype comprise the largest subgroup ( approximately 50%) but have a highly heterogeneous clinical course. By multi-parameter flow cytometry we analyzed CD7 expression along with other phenotypic markers in 185 patients with normal-karyotype AML. CD7 was expressed in 68 (37%) patients. CD7 expression was associated with younger age (P=0.024) but not with sex, WBC count, or extramedullary disease. Patients expressing CD7 had significant shorter disease free (DFS) and post-remission survivals (PRS) than patients without CD7 (DFS of 12 months versus 42 months, P=0.005; PRS of 15 months versus 33 months, P=0.013). We also found that expression of CD34 or HLA-DR was associated with lower CR rate (P=0.0007 and P=0.019, respectively) but did not affect DFS or OS. Furthermore, as for all AML patients, we demonstrated that in the normal karyotypic subgroup, patients with higher WBC counts (>50) and older age (>60 years) had lower CR rate (P=0.003 and P=0.0157, respectively) and shorter OS (P

Assuntos
Antígenos CD7/biossíntese , Antígenos CD7/genética , Regulação Leucêmica da Expressão Gênica , Leucemia Mieloide/genética , Doença Aguda , Adulto , Idoso , Idoso de 80 Anos ou mais , Intervalo Livre de Doença , Feminino , Citometria de Fluxo/métodos , Humanos , Imunofenotipagem , Cariotipagem , Leucemia Mieloide/terapia , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Indução de Remissão , Taxa de Sobrevida , Resultado do Tratamento
12.
Am J Hematol ; 82(1): 73-6, 2007 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-17019687

RESUMO

By multiparameter flow cytometry, the T-cell-associated markers CD4 and CD7 were aberrantly coexpressed on a case of B-cell chronic lymphocytic leukemia (CLL). The CLL had an immunophenotype: CD19+, CD20+, CD79b+, CD5+, CD23+, FMC7+, kappa+, CD4+, and CD7+. Molecular analysis confirmed clonal rearrangement of the immunoglobin heavy chain genes and a germline configuration of the T-cell receptor genes. Fluorescence in situ hybridization showed trisomy 12 anomaly. There was no evidence of deletion 17p (p53), 11q23 (ATM), or 13q14 or translocation t(11:14). The patent was clinically stable without treatment. Although the pathogenesis of such aberrant immunophenotype remains to be determined, the current case illustrates the phenotypic heterogeneity of CLL, and emphasizes the importance of a comprehensive diagnostic approach including clinical, morphologic, immunophenotypic, and molecular cytogenetic studies.


Assuntos
Antígenos CD7/biossíntese , Biomarcadores Tumorais/biossíntese , Antígenos CD4/biossíntese , Regulação Leucêmica da Expressão Gênica , Leucemia Linfocítica Crônica de Células B/metabolismo , Idoso , Antígenos CD/biossíntese , Antígenos CD/genética , Antígenos CD7/genética , Biomarcadores Tumorais/genética , Antígenos CD4/genética , Citometria de Fluxo , Rearranjo Gênico de Cadeia Pesada de Linfócito B/genética , Rearranjo Gênico do Linfócito T/genética , Glicoproteínas/biossíntese , Glicoproteínas/genética , Humanos , Imunofenotipagem , Leucemia Linfocítica Crônica de Células B/diagnóstico , Leucemia Linfocítica Crônica de Células B/genética , Leucemia Linfocítica Crônica de Células B/patologia , Masculino
13.
Blood ; 108(7): 2399-406, 2006 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-16778138

RESUMO

Neoplastic T cells in mycosis fungoides (MF) are resistant to apoptotic agents, including galectin-1 that is abundant in skin. Although MF cells are typically CD7-, and thus galectin-1 resistant, CD7+ HH cells, derived from a patient with MF, were also resistant to galectin-1. HH cells demonstrate altered cell surface glycosylation, with loss of core 2 O-glycan ligands for galectin-1 created by core 2 beta1,6-N-acetylglucosaminyltransferase (C2GnT-I). Loss of core 2 O-glycans on tumor cells was also seen in primary CD7+ MF lesions. Surprisingly, HH cells are heterozygous for a C2GnT-I point mutation, yet this mutation resulted in a dramatic reduction in cellular glycosyltransferase activity. Expression of wild-type C2GnT-I in human HH cells, or murine lymphoma cells that lack C2GnT-I, restored core 2 O-glycan expression and susceptibility to galectin-1, whereas mutant enzyme lacked activity and did not restore core 2 O-glycan expression or susceptibility to galectin-1. Mutant enzyme did not have a dominant negative effect by affecting dimerization or activity of wild-type enzyme; rather, C2GnT-I haploinsufficiency is sufficient for loss of core 2 O-glycan expression and galectin-1 resistance. Thus, glycosyltransferase haploinsufficiency results in altered cellular glycosylation and resistance to cell death, identifying a new survival mechanism for T-lymphoma cells.


Assuntos
Apoptose , Linfoma de Células T/enzimologia , Linfoma de Células T/patologia , N-Acetilglucosaminiltransferases/genética , N-Acetilglucosaminiltransferases/fisiologia , Antígenos CD7/biossíntese , Sequência de Bases , Linhagem Celular Tumoral , Sobrevivência Celular , Galectina 1/química , Predisposição Genética para Doença , Heterozigoto , Humanos , Linfoma de Células T/genética , Dados de Sequência Molecular , Mutação de Sentido Incorreto , Pele/metabolismo
14.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 14(1): 39-41, 2006 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-16584588

RESUMO

To evaluate the significance of immunologic classification for typing of acute leukemia (AL). 68 cases of AL were classified by morphologic and immunologic typings. The results showed that the consistency rate was 94.1% between morphology and immunology, and 4 morphologic misdiagnosed cases were corrected by immunology; CD13 and CD33 were special myeloid lineage-associated antigens; AML-M(3) was often CD34 low-expressed and HLA-DR-negative; CD14 was often expressed in AML-M(4) and M(5); lymphoid lineage-associated antigens (CD7) were easily found in ANLL, and myeloid lineage-associated antigens were also found in ALL. In conclusion, immunologic classification can improve the accuracy in acute leukemia diagnosis. The diagnosis of some special AL, such as acute unidentified leukemia (AUL), AML-M(0) and so on, must rely on immunologic classification.


Assuntos
Antígenos CD/biossíntese , Imunofenotipagem , Leucemia Mieloide Aguda/classificação , Leucemia-Linfoma Linfoblástico de Células Precursoras/classificação , Adolescente , Adulto , Idoso , Antígenos CD34/biossíntese , Antígenos CD7/biossíntese , Antígenos de Diferenciação Mielomonocítica/biossíntese , Antígenos CD13/biossíntese , Feminino , Humanos , Leucemia Mieloide Aguda/imunologia , Receptores de Lipopolissacarídeos/biossíntese , Masculino , Pessoa de Meia-Idade , Leucemia-Linfoma Linfoblástico de Células Precursoras/imunologia , Lectina 3 Semelhante a Ig de Ligação ao Ácido Siálico
15.
Leuk Res ; 30(8): 957-63, 2006 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-16417922

RESUMO

There is an emerging use of flow cytometry to evaluate patients with myelodysplastic syndrome (MDS). We have studied CD7 and TdT expression in the CD34+ myeloid blast cell population in 55 bone marrow samples of patients with MDS. CD7 and/or TdT were detected in 38 out of 55 patients (69%). CD7 expression was not related to other bad prognosis data but conversely, we found an association between TdT+ CD34 myeloblasts and high-risk MDS patients according to the International Prognostic Scoring System. Therefore, CD7 and TdT may help to establish the diagnosis of MDS and, TdT expression also seems to be a useful marker in distinguishing risk groups.


Assuntos
Antígenos CD34/biossíntese , Antígenos CD7/biossíntese , DNA Nucleotidilexotransferase/biossíntese , Células Precursoras de Granulócitos/imunologia , Síndromes Mielodisplásicas/imunologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Antígenos CD7/análise , Análise Citogenética , DNA Nucleotidilexotransferase/análise , Feminino , Citometria de Fluxo , Seguimentos , Humanos , Imunofenotipagem , Masculino , Pessoa de Meia-Idade , Síndromes Mielodisplásicas/diagnóstico , Fatores de Risco , Taxa de Sobrevida
16.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 13(5): 812-4, 2005 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-16277848

RESUMO

To explore CD34(+) antigen expression in new diagnosed acute myeloid leukemia (AML) and analyze the prognosis for CD34(+) AML patients, the expression of antigen CD34 in 238 AML patients was detected by indirect immunofluorescence assay. The results showed that CD34 in 92 out of the 238 patients (38.7%) were positive, there was relationship between the CD34(+) expression and FAB subtypes (M(0), M(1)), and no CD34(+) expression was observed in M(3) subtypes. The complete remission rate of CD34(+) AML patients was 32%, which was lower than that of CD34(-) AML (61%). The lymphoid-associated antigen (CD7) was significantly increased in CD34(+) AML patients, compared with CD34(-) patients (P < 0.05). It is concluded that CD34(+) AML patients show poor prognosis and lower CR rate. The detection of CD34 expression is of some value in predicting prognosis in AML.


Assuntos
Antígenos CD34/biossíntese , Leucemia Monocítica Aguda/patologia , Leucemia Mieloide Aguda/patologia , Leucemia Mielomonocítica Aguda/patologia , Adolescente , Adulto , Idoso , Antígenos CD7/biossíntese , Feminino , Técnica Indireta de Fluorescência para Anticorpo , Humanos , Leucemia Monocítica Aguda/metabolismo , Leucemia Mieloide Aguda/metabolismo , Leucemia Mielomonocítica Aguda/metabolismo , Masculino , Pessoa de Meia-Idade , Prognóstico
17.
Proteomics ; 5(18): 4719-32, 2005 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-16247730

RESUMO

T helper cells (Th) are divided into Th1 and Th2 subsets based upon their cytokine profiles and function. Naïve Th cells differentiate into Th1 and Th2 subsets depending on the antigens, costimulatory molecules, and cytokines they encounter. Cytokine interleukin (IL)-12 enhances the generation of Th1 lymphocytes and inhibits the production of Th2 subset. Many genes involved in Th cell differentiation have already been identified at transcriptomic level in microarray studies. In this study, isotope coded affinity tag labeling combined with chromatographic techniques and tandem mass spectrometry was used to find IL-12 regulated proteins in the microsomal fraction of Th cells. A total of 380 and 275 proteins were initially identified and quantitated in two experiments. After the high-confidence protein identifications were restricted to those where at least two different peptides were identified per protein, and these confirmed by manual inspection of the tandem mass spectra, 147 proteins remained. Of these high-confidence protein identifications 41 had at least 1.5-fold change in expression between IL-12 treated and nontreated cells. Among the differentially regulated proteins were galectin-1 (gal-1) and CD7, and their down-regulation was further corroborated with Western blotting and flow cytometry, respectively. Gal-1 and CD7 are known to interact with each other, and regulate immunity through influencing apoptosis and cytokine production. Our data indicate that IL-12 down-regulates the expression of both gal-1 and CD7 in the microsomal fraction of peripheral blood mononuclear cells and cord blood CD4(+) cells. The down-regulation of these proteins is likely to have a role in specific Th cell selection and cytokine environment creation.


Assuntos
Regulação para Baixo , Galectina 1/biossíntese , Interleucina-12/fisiologia , Proteoma/química , Células Th1/metabolismo , Células Th2/metabolismo , Antígenos CD7/biossíntese , Proteínas Sanguíneas/análise , Proteínas Sanguíneas/genética , Regulação da Expressão Gênica , Humanos , Marcação por Isótopo , Microssomos/efeitos dos fármacos , Microssomos/metabolismo , Fosforilação , Fator de Transcrição STAT4/metabolismo
18.
Exp Mol Pathol ; 79(1): 39-41, 2005 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-16005710

RESUMO

The prognostic significance of selected markers of leukemic cells is well known. CD7 and CD56 expression at diagnosis has been associated with low remission rates and biological aggressiveness in a significant proportion of acute leukemias. Among 46 patients with acute myeloid leukemia, we found CD7 expression in 15 cases (32.6%) and CD56 positivity in 10 patients (21.7%). Six of these myeloid leukemia cases (13%) showed expression of both CD7 and CD56. Four of 46 (8.7%) patients expressed CD79a. Among the 10 that were acute myeloblastic leukemia, 8 expressed CD7, 4 expressed CD56, and 4 were positive for CD79a. Thus, these markers were expressed early in hemopoietic ontogeny in the lesser-differentiated acute myeloid leukemia subtypes, including FAB M0, M1, and M2. Whereas CD7 and CD56 were each positive in 4 cases of acute myelomonocytic leukemia (FAB M4 subtype), there was no CD79a expression in the M4 cases. CD7 is expressed by mature T cells, NK cells, and an immature myeloid cell subset. NK cells and a T cell subset express CD56. By contrast, CD79a is a B cell marker that is assigned a high score of 2.0 in the differentiation of acute leukemias of ambiguous lineage in the WHO classification. The aberrant expression of CD7, CD56, and CD79a, representing the capacity of these leukemias for trilineal expression of leukocyte differentiation antigens, portends a poor prognosis.


Assuntos
Antígenos CD7/biossíntese , Antígenos CD/biossíntese , Biomarcadores Tumorais/análise , Antígeno CD56/biossíntese , Leucemia Mieloide Aguda/metabolismo , Receptores de Antígenos de Linfócitos B/biossíntese , Antígenos CD79 , Humanos , Leucemia Mieloide Aguda/patologia , Prognóstico
19.
J Immunol ; 174(9): 5414-22, 2005 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-15843540

RESUMO

We have identified a large population of CD3(-)7(+) cells in human fetal gut. Three- and four-color flow cytometry revealed a distinct surface Ag profile on this population; the majority were negative for CD4 and CD8, whereas most of the remainder expressed the CD8alphaalpha homodimer. In contrast about half of CD3(+) cells expressed CD4 and half expressed CD8alpha. A large proportion of CD3(-)7(+) cells expressed CD56, CD94, and CD161, and whereas CD3(+) T cells also expressed CD161, they only rarely expressed CD56 or CD94. Further studies were conducted to determine whether the CD3(-)7(+) cells have the potential to differentiate into CD3(+) cells. About half of CD3(-)7(+) cells contain intracellular CD3epsilon. Rearranged TCR gamma-chains were detected in highly purified CD3(-)7(+) cells as an early molecular sign of T cell commitment, and the pattern of rearrangement with V regions spliced to the most 5' Jgamma segment is reminiscent of early thymocyte differentiation. In reaggregate thymic organ cultures, CD3(-)7(+) cells also gave rise to CD3(+) T cells. Thus, we demonstrate that the CD3(-)7(+) cells present in the human fetal gut display a distinct phenotype and are able to develop into CD3(+) T cells.


Assuntos
Antígenos CD7/biossíntese , Complexo CD3 , Diferenciação Celular/imunologia , Imunofenotipagem , Mucosa Intestinal/citologia , Mucosa Intestinal/imunologia , Subpopulações de Linfócitos T/citologia , Subpopulações de Linfócitos T/imunologia , Adulto , Envelhecimento/imunologia , Complexo CD3/biossíntese , Complexo CD3/genética , Complexo CD3/metabolismo , Membrana Celular/imunologia , Membrana Celular/metabolismo , Senescência Celular/imunologia , Colo/citologia , Colo/imunologia , Colo/metabolismo , Feto , Rearranjo Gênico da Cadeia gama dos Receptores de Antígenos dos Linfócitos T , Humanos , Íleo/citologia , Íleo/imunologia , Íleo/metabolismo , Mucosa Intestinal/metabolismo , Líquido Intracelular/imunologia , Líquido Intracelular/metabolismo , Células Matadoras Naturais/imunologia , Células Matadoras Naturais/metabolismo , Técnicas de Cultura de Órgãos , Receptores Imunológicos/biossíntese , Subpopulações de Linfócitos T/metabolismo , Timo/citologia , Timo/imunologia , Timo/metabolismo
20.
J Immunol ; 171(12): 6900-9, 2003 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-14662897

RESUMO

Cross-presentation of self Ags by APCs is key to the initiation of organ-specific autoimmunity. As MHC class I molecules are essential for the initiation of diabetes in nonobese diabetic (NOD) mice, we sought to determine whether the initial insult that allows cross-presentation of beta cell autoantigens in diabetes is caused by cognate interactions between naive CD8(+) T cells and beta cells. Naive splenic CD8(+) T cells from transgenic NOD mice expressing a diabetogenic TCR killed peptide-pulsed targets in the absence of APCs. To ascertain the role of CD8(+) T cell-induced beta cell lysis in the initiation of diabetes, we expressed a rat insulin promoter (RIP)-driven adenovirus E19 transgene in NOD mice. RIP-E19 expression inhibited MHC class I transport exclusively in beta cells and rendered these cells resistant to lysis by CD8(+) (but not CD4(+)) T cells, both in vitro and in vivo. Surprisingly, RIP-E19 expression impaired the accumulation of CD8(+) T cells in islets and delayed the onset of islet inflammation, without affecting the timing or magnitude of T cell cross-priming in the pancreatic lymph nodes, which is the earliest known event in diabetogenesis. These results suggest that access of beta cell autoantigens to the cross-presentation pathway in diabetes is T cell independent, and reveal a previously unrecognized function of MHC class I molecules on target cells in autoimmunity: local retention of disease-initiating clonotypes.


Assuntos
Apresentação de Antígeno , Autoantígenos/metabolismo , Linfócitos T CD8-Positivos/imunologia , Comunicação Celular/imunologia , Citotoxicidade Imunológica , Diabetes Mellitus Tipo 1/imunologia , Ilhotas Pancreáticas/imunologia , Animais , Apresentação de Antígeno/genética , Antígenos CD7/biossíntese , Linfócitos T CD4-Positivos/imunologia , Linfócitos T CD4-Positivos/patologia , Linfócitos T CD8-Positivos/metabolismo , Comunicação Celular/genética , Movimento Celular/genética , Movimento Celular/imunologia , Cruzamentos Genéticos , Citotoxicidade Imunológica/genética , Diabetes Mellitus Tipo 1/genética , Diabetes Mellitus Tipo 1/patologia , Progressão da Doença , Proteína Ligante Fas , Feminino , Interfase/genética , Interfase/imunologia , Ilhotas Pancreáticas/metabolismo , Ilhotas Pancreáticas/patologia , Ligantes , Linfonodos/imunologia , Linfonodos/metabolismo , Linfonodos/patologia , Ativação Linfocitária/genética , Masculino , Glicoproteínas de Membrana/imunologia , Glicoproteínas de Membrana/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos NOD , Camundongos Transgênicos , Transporte Proteico/genética , Transporte Proteico/imunologia , Receptores de Antígenos de Linfócitos T/genética , Receptor fas/metabolismo
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