Human axillary secretions influence women's menstrual cycles: the role of donor extract from men.

Menstrual cycle lengths of 29.5 +/- 3 days ("normal cycles") are more frequent in women who have weekly coital activity than in women who do not. In order to investigate potential mechanisms controlling the association between heterosexual activity and menstrual cycle length, and in light of the nonhuman literature suggesting that a chemical signal from males could be involved, menstrual cycle lengths of nulliparous women were evaluated following regular application of axillary extract from donor males. Compared to controls receiving only blank/ethanol applications, women receiving axillary extracts for 12.5 to 14.5 weeks showed the following changes: a reduced incidence in variability of cycle lengths; and a reduced proportion of aberrant length cycles.

Human axillary secretions influence women's menstrual cycles: the role of donor extract of females.

Menstrual synchrony in human females has previously been demonstrated among women attending a predominantly female university as well as among women attending coeducational universities. In each of these studies, women who spent the most time together were most likely to show the menstrual synchrony. In this experiment, the possibility that substances in axillary secretions might mediate this effect was tested using a prospective, double-blind research design and a combined axillary extract from a group of female donors. Female subjects who reported themselves to have normal (29.5 +/- 3 day) cycles were exposed to the axillary extracts or blank/ethanol for 10 to 13 weeks. Recipients of the axillary extracts showed a significant reduction in "days' difference in menses onset" relative to the donor cycle, no change was evident for recipients of blank/ethanol. These results demonstrate that constituents from the axillary region of donor females can shift the time of menstrual onset of another group to conform with the donors' cycle and that this effect can occur even in the absence of social contact.

Axillary 5 alpha-androst-16-en-3-one, cholesterol and squalene in men; preliminary evidence for 5 alpha-androst-16-en-3-one being a product of bacterial action.

Twenty-four hour axillary levels of the odorous steroid 5 alpha-androst-16-en-3-one, have been measured in six men by radioimmunoassay. Initially, no control of bacterial activity was made and conditions attempting a normal axillary environment were maintained. The level of 5 alpha-androst-16-en-3-one was significantly higher (P = 0.028) in one axilla ("superior") than the other ("inferior") and levels showed considerable variation both between and within individuals. This difference between axillae was also observed for cholesterol (P = 0.0013) but not for squalene (P = 0.18). This suggests that the presence of 5 alpha-androst-16-en-3-one in the axilla does not correlate with sebum secretion. The effect of a general germicidal agent was tested by shaving and applying Povidone-iodine to the "superior" axilla whilst treating the "inferior" axilla as a control. A highly significant drop in the level of 5 alpha-androst-16-en-one in the "superior" axilla below that in the control axilla was obtained (P = 0.000014, double tailed, as calculated using the Fisher Exact test). Squalene and cholesterol were measured in an attempt to monitor glandular activity and their levels were not significantly affected by Povidone-iodine. It is likely, therefore, that the production of 5 alpha-androst-16-en-3-one is from metabolism of a percursor in the axillae by skin micro-organisms.

Aromatization of androgens by human abdominal and breast fat tissue.

The ability of human abdominal, breast and axillary fat to convert androgens into estrogens was investigated by incubating labeled substrates in the presence of NADPH with a variety of cell preparations. The incubation products were subjected to phenolic partition, paper chromatography, methyl-ether formation, repeat chromatography and crystallization with cold carrier reference standards to constant specific activity. Androstenedione was converted to estrone and, to a lesser extent, to 17beta-estradiol by crude homogenates, minces, fat-free particulate fractions (1,000-100,000 time g) and isolated fat cells obtained from abdominal, breast or axillary fat. Testosterone was found to be aromatized as actively as androstenedione, but inthis case more 17 beta-estrodiol was formed than estrone. 19-Hydroxyandrostenedione-2 also served as substrate, givingresults similar to those obtained with androstenedione. Fat tissue obtained from cancerous breasts was found to be as active as normal breast fat (1-4 pg/g fat/90 min) and within the range found for abdominal fat (1-27 pg/g fat/90 min). In each case in which axillary fat was compared to breast fat from the same subject, the activity of the axillary fat was 5 to 10 times higher. The results indicate a possible role of adipose tissue as a significant extra-gonadal source of estrogens.