[Urethro-adnexitis of the male and sexually transmissible pathogens. A report of experiences of the Giessen study group]. / Urethro-Adnexitis des Mannes und sexuell übertragbare Erreger. Ein Erfahrungsbericht der Giessener Arbeitsgruppe.

In recent years, the improvement in microbiological diagnostic methods has caused the spectrum of infectious agents causing male urethroadnexitis to expand considerably. In addition to the well-known urinogenic enterobacteriae and enterococci and the sexually transmitted gonococci and trichomonads, Chlamydia trachomatis and Ureaplasma urealyticum must now be considered particularly important etiologic agents that are sexually transmitted. Their cell biology and epidemiology, our diagnostic procedures, and the criteria of etiologic classification of male urethroadnexitis are described in detail. The recently developed quinolone derivates offer new and promising therapeutic perspectives.

Transmission and attempted isolation of the etiologic agent associated with lymphofollicular hyperplasia of the canine species.

From 18 donor dogs of different breeds and ages, follicular lesions of the third eyelid (plica senilunaris conjunctiva) and genitalia were surgically removed, trypsinized, and inoculated on monolayers of HeLa, rabbit kidney, and canine kidney cell cultures. Blind passages of the lesion material were made every 96 hours for 10 to 15 cell culture passages. Cellular suspensions prepared from the lesions were grown in test tubes and passaged 3 times at 10-day intervals between passages. All cultures were observed each day for cytopathic effect. Transmission studies were made by (1) inoculating normal pups with cellular suspensions of the lesions from an infected dog and an infected pup, (2) placing normal pups in contact with infected ones for contact transmission, and (3) inoculating normal animals with cell suspensions prepared from inoculated monolayers. Cytopathic changes were not seen in any of the cell culture monolayers. All transmission attempts were successful, in that characteristic lesions comparable in appearance to those seen in natural infections were produced in susceptible pups. The lesion material from an infected pup was found to be infective for a normal pup after 6 passages in tissue culture (primary rabbit kidney cells) despite absence of cytopathic effect.