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1.
J Pharm Biomed Anal ; 246: 116252, 2024 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-38788622

RESUMO

Balanophora harlandii Hook (B. harlandii), a folk medicine, has been traditionally employed to treat traumatic bleeding, gastroenteritis, icteric hepatitis, hemorrhoids, and other conditions. In this work, polysaccharides with anti-inflammatory effects were extracted from B. harlandii and purified. The extraction conditions were optimized, and the properties of one purified neutral fraction, denoted as BHPs-W-S3, were analyzed. BHPs-W-S3 has a molecular weight of 14.1 kDa, and its three main monosaccharides are glucose, galactose, and xylose, with a molar ratio of 6.4:1.7:1.1. Its main chain consists of →6)-α-D-Glcp-(1→, →4,6)-α-D-Glcp-(1→, →6)-ß-D-Galp-(1→, →3,6)-ß-D-Galp-(1→, and it has branch chains at the O-4 and/or O-3 positions. In addition, in vitro experiments showed that the polysaccharides from B. harlandi can decrease the phosphorylation level of p65 and IκBα in LPS-induced RAW264.7 cells to reduce the expression of the pro-inflammatory genes such as TNF-α, IL-6, and IL-1ß.


Assuntos
Anti-Inflamatórios , Polissacarídeos , Camundongos , Animais , Polissacarídeos/farmacologia , Polissacarídeos/química , Polissacarídeos/isolamento & purificação , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/química , Anti-Inflamatórios/isolamento & purificação , Células RAW 264.7 , Peso Molecular , Balanophoraceae/química , Extratos Vegetais/farmacologia , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Lipopolissacarídeos/farmacologia , NF-kappa B/metabolismo , Fator de Necrose Tumoral alfa/metabolismo , Inflamação/tratamento farmacológico
2.
Artigo em Inglês | MEDLINE | ID: mdl-35021136

RESUMO

An UHPLC/LC-MS was founded to detect balanophorin B (B), gallic acid (GA), 4-hydroxycinnamic acid (HC), and their in vivo profiling in rats, after oral administration of the ethanol extract of Balanophora simaoensis S. Y. Chang et Tam. The in vivo dynamic existence of 3 molecular entities in rats and the multistep biotransformation of GA were elucidated by their sensitive mass spectrometry response after efficient UHPLC and/or HPLC separation, through analyzing the bio-samples of rat plasma, bile, liver, kidneys, and excreta. The method was validated with satisfactory calibration curves having correlation coefficients r from 0.996 to 0.999 for concentration scaled from 0.100 nM to 0.100 µM, internal standard normalized matrix factors ranged from 0.923 to 0.993, sextuplicate recoveries valued from 95.0% to 103.6%, as well as accuracy and precision varied from 95.6% to 103.7%. The content of B, GA, and HC in the whole herb was of 4.66, 63.5, and 10.4 µmol/kg in dry weight, respectively. The Cmax for B, GA, and HC in rat systemic circulation was of 76.0 nM, 2.30 µM, and 51.0 µM, with tmax at 3, 2, and 2 h, respectively. B and GA stayed in rat liver over 4 hs to present a material base for the pharmacology and pharmacodynamics of the whole herb. The biotransformation of GA indicated a complicated scheme in rats. As a final metabolite from GA with total biotransformation conversion over 20%, 4-hydroxybenzaldehyde resourced from two steps of dehydroxylation and one step of reduction of GA, but not concerned with HC.


Assuntos
Balanophoraceae , Ácidos Cumáricos , Medicamentos de Ervas Chinesas , Ácido Gálico , Animais , Masculino , Ratos , Administração Oral , Balanophoraceae/química , Cromatografia Líquida de Alta Pressão/métodos , Ácidos Cumáricos/administração & dosagem , Ácidos Cumáricos/sangue , Ácidos Cumáricos/farmacocinética , Medicamentos de Ervas Chinesas/administração & dosagem , Medicamentos de Ervas Chinesas/análise , Medicamentos de Ervas Chinesas/farmacocinética , Ácido Gálico/administração & dosagem , Ácido Gálico/sangue , Ácido Gálico/farmacocinética , Espectrometria de Massas/métodos , Ratos Sprague-Dawley
3.
Bol. latinoam. Caribe plantas med. aromát ; 20(6): 598-610, nov. 2021. tab
Artigo em Inglês | LILACS | ID: biblio-1369781

RESUMO

Lophophytum species are holoparasites that grow on tree roots. The objectives of the work were to explore the chemical composition of the tubers of two Lophophytum species and to analyze the antioxidant, anti-inflammatory and antilithiatic activity of their extracts using in vitro methods. The chemical composition was determined by histochemical, phytochemical and TLC tests. In addition, the profile of phenolic compounds was determined by HPLC-MS. The presence of secondary metabolites of recognized activity was demonstrated. The results of the HPLC-MS/MS allowed the tentative identification of catechin, luteolin and glycosides of eriodictyol, naringenin and luteolin in the extract of Lophophytum leandriand eriodictyol, naringenin, luteolin and their glycosylated derivatives in Lophophytum mirabile. The extracts showed promising antioxidant (DPPH, ABTS and ß-carotene-linoleic acid), anti-inflammatory (inhibition of 5-LOX) and anti-urolytic (by bioautographic TLC) activity. It is noteworthy that these are the first results of the phytochemical composition and biological activity of L. mirabile. However, in vivo studies are required to corroborate these activities.


Las especies de Lophophytumson holoparásitas que crecen en raíces de árboles. Los objetivos del trabajo fueron explorar la composición química del túber de dos especies de Lophophytum y analizar la actividad antioxidante, antiinflamatoria y antilitiásica de sus extractos usando métodos in vitro. La composición química se determinó mediante pruebas histoquímicas, fitoquímicas y por TLC. Además, se determinó el perfil de compuestos fenólicos por HPLC-MS/MS. Se demostró presencia de metabolitos secundarios de reconocida actividad. Los resultados del HPLC-MS/MS permitieron identificar tentativamente catequina, luteolina y glucósidos de eriodictiol, naringenina y luteolina en el extracto de Lophophytum leandriy eriodictiol, naringenina, luteolina y sus derivados glicosilados en Lophophytum mirabile. Los extractos mostraron prometedora actividad antioxidante (DPPH, ABTS y ß-caroteno-ácido linoleico), antiinflammatoria (inhibición de la 5-LOX) y antiurolitiásica (por TLC bioautográfica). Es de destacar que estos son los primeros resultados de composición fitoquímica y actividad biológica de L. mirabile. Sin embargo, se requieren estudios in vivo para corroborar dichas actividades.


Assuntos
Extratos Vegetais/farmacologia , Extratos Vegetais/química , Balanophoraceae/química , Cromatografia Líquida de Alta Pressão , Flavanonas/análise , Flavonas/análise , Compostos Fenólicos/análise , Espectrometria de Massas em Tandem , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/química , Antioxidantes/farmacologia , Antioxidantes/química
4.
J Cell Mol Med ; 25(6): 2828-2840, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33507617

RESUMO

Renal interstitial fibrosis (RIF) is a crucial pathological change leading to chronic kidney disease (CKD). Currently, no effective medicines have been available for treating it. In our research, we examined the effects of polysaccharides extracted from Balanophora polyandra Griff (BPPs) on kidney fibrosis and epithelial to mesenchymal transition (EMT) in vivo and in vitro, aiming to explore the underlying mechanisms. By using the mice with unilateral urethral obstruction (UUO) as experimental subjects, we examined the medicinal values of BPPs on alleviating RIF. The effects of BPPs were evaluated by examining the histological staining and relative mRNA and protein expression levels of the related genes. The possible underlying mechanisms were further explored with human normal renal proximal tubular epithelia (HK-2 cells) as in vitro model. In UUO mice, BPP treatment could significantly alleviate interstitial fibrosis through reducing the components (Collagens I, III and IV) of extracellular matrix (ECM), and reducing the activation of fibroblasts producing these components, as revealed by inhibiting the hallmarks (fibronectin and α-SMA) of fibroblast activation. Furthermore, BPP administration increased the expression levels of matrix metalloproteinases (MMPs) and declined those of tissue inhibitors of metalloproteinases (TIMPs). BPPs markedly ameliorated EMT in both the kidneys of UUO mice and TGF-ß1 treated HK-2 cells. Moreover, BPP treatment decreased the expression levels of several transcriptional factors involved in regulating E-cadherin expression, including snail, twist and ZEB1. Additionally, the Hedgehog pathway was found to be closely correlated with renal fibrosis and EMT. Altogether, our results clearly demonstrated that BPP treatment effectively inhibited the Hedgehog pathway both in renal tissues of UUO mice and TGF-ß1-treated HK-2 cells. Thus, BPPs ameliorated RIF and EMT in vivo and in vitro via suppressing Hedgehog signalling pathway.


Assuntos
Balanophoraceae/química , Transição Epitelial-Mesenquimal/efeitos dos fármacos , Proteínas Hedgehog/metabolismo , Nefropatias/metabolismo , Polissacarídeos/farmacologia , Transdução de Sinais/efeitos dos fármacos , Animais , Linhagem Celular , Colágeno/metabolismo , Modelos Animais de Doenças , Células Epiteliais/efeitos dos fármacos , Células Epiteliais/metabolismo , Matriz Extracelular/metabolismo , Fibrose , Humanos , Nefropatias/tratamento farmacológico , Nefropatias/etiologia , Nefropatias/patologia , Túbulos Renais Proximais , Masculino , Camundongos , Polissacarídeos/química , Fator de Crescimento Transformador beta1/metabolismo
5.
Life Sci ; 267: 118910, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-33359671

RESUMO

AIMS: Cancer cells exhibit a metabolic change called aerobic glycolysis compared with normal cells. Balanophorin B is a terpenoid ingredient reported from the genus Balanophora. In this research, we studied the effect of balanophorin B on glycolysis of HepG2 cells and Huh-7 cells under hypoxia. MAIN METHODS: The Warburg effect was monitored by assessing glucose uptake, oxygen consumption rate (OCR) and extracellular acidification rate (ECAR). Key enzymes in the glycolytic pathway and HIF-1α protein expression and degradation were analyzed by real-time PCR and western blotting. The anti-cancer effect of balanophorin B in vivo was also investigated. KEY FINDINGS: Balanophorin B inhibited the proliferation, glucose uptake, and ECAR in both HepG2 cells and Huh-7 cells. In addition, balanophorin B inhibited the protein level of HIF-1α and its downstream targets LDHA and HKII under hypoxia, whereas HIF-1α mRNA level did not change after balanophorin B treatment. The HIF-1α plasmid reversed the inhibition of balanophorin B on glycolysis, and the proteasome inhibitor MG132 attenuated the effect of balanophorin B on HIF-1α protein expression, suggesting that balanophorin B might post-transcriptionally affect HIF-1α. Moreover, balanophorin B increased the expression of VHL and PHD2. HIF-1α siRNA also greatly attenuated the inhibitory effect of balanophorin B on HepG2 cells glucose uptake. Balanophorin B significantly inhibited tumor growth in vivo, without causing obvious toxicity to mice. SIGNIFICANCE: These data suggest that balanophorin B inhibits glycolysis probably via an HIF-1α-dependent pathway, and the ubiquitin-proteasome pathway was greatly involved in the induction of balanophorin B on HIF-1α degradation.


Assuntos
Glicólise/efeitos dos fármacos , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Terpenos/farmacologia , Animais , Balanophoraceae/química , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Ciclo do Ácido Cítrico , Glucose/metabolismo , Células Hep G2 , Humanos , Camundongos , Camundongos Nus , Extratos Vegetais/farmacologia , RNA Mensageiro/metabolismo , Transdução de Sinais/efeitos dos fármacos , Ensaios Antitumorais Modelo de Xenoenxerto
6.
Nat Prod Res ; 35(11): 1825-1829, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31359786

RESUMO

Two new butenolides, balanolides A (1) and B (1), were isolated from the aerial parts of the parasitic plant Balanophora fungosa. Their structures were established by comprehensive spectroscopic analysis and quantum chemical ECD calculation. The new butenolides were evaluated for their inhibitory effects against nitric oxide (NO) production in LPS-induced RAW 264.7 macrophage cells. Compounds 1 and 2 displayed moderate anti-inflammatory activity with IC50values of 11.8 and 12.9 µM, respectively.


Assuntos
4-Butirolactona/análogos & derivados , Anti-Inflamatórios/farmacologia , Balanophoraceae/química , 4-Butirolactona/farmacologia , Animais , Lipopolissacarídeos/farmacologia , Macrófagos/efeitos dos fármacos , Camundongos , Óxido Nítrico/biossíntese , Extratos Vegetais/química , Células RAW 264.7
7.
Biomed Chromatogr ; 34(12): e4949, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32678491

RESUMO

Balanophora involucrata J. D. Hooker has been known to possess potential anti-inflammatory and antibacterial activities; however, its antiviral activity has not been evaluated so far. In order to find new neuraminidase inhibitors (NAIs), the neuraminidase (NA) inhibition activity of different B. involucrata extracts was evaluated. In this study, an in vitro NA inhibition assay was performed to identify which extract of B. involucrata exhibits (maximal) inhibitory activity against NA. Ultra high performance liquid chromatography/quadrupole time-of-flight-tandem mass spectroscopy (MS/MS) and molecular docking techniques were used to identify the specific compounds responsible for the anti-influenza activity of the extract, and to explore the potential natural NAIs. The ethyl acetate extract of B. involucrata exhibited significant inhibitory activity against NA with 50% inhibitory concentration (IC50 ) value of 159.5 µg/mL. Twenty compounds were identified according to the MS/MS spectra; among them two compounds (quercitrin and phloridzin) showed obvious inhibitory activity against NA, with IC50 of 311.76 and 347.32 µmol/L, respectively. This study suggested that B. involucrata can be a potential natural source of NAIs and may be useful in the fight against ferocious influenza viruses.


Assuntos
Balanophoraceae/química , Inibidores Enzimáticos , Neuraminidase/antagonistas & inibidores , Extratos Vegetais/química , Cromatografia Líquida de Alta Pressão , Inibidores Enzimáticos/análise , Inibidores Enzimáticos/isolamento & purificação , Inibidores Enzimáticos/farmacologia , Flavonoides/análise , Flavonoides/isolamento & purificação , Flavonoides/farmacologia , Simulação de Acoplamento Molecular , Espectrometria de Massas em Tandem
8.
J Sep Sci ; 43(2): 524-530, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31652014

RESUMO

Thonningia sanguinea is a parasitic herb widely used in traditional African medicine. Dihydrochalcone glucosides (unsubstituted, substituted with hexahydroxydiphenoyl or galloyl moieties) are the main constituents in the subaerial parts of this plant. In the present study, purification of the six major compounds from a methanol extract of the plant's subaerial parts was achieved by centrifugal partition chromatography. A first dimension centrifugal partition chromatography separation with the solvent system methyl tert-butyl ether/1,2-dimethoxyethane/water (1:2:1) in the ascending mode enabled the isolation of the two major bioactive compounds thonningianin A and B from 350 mg of methanol extract within only 16 min with respectable yields (25.7 and 21.1 mg), purities (87.1 and 85%), and recoveries (71.2 and 70.4%). Using a multiple heart-cutting strategy, the remaining four major dihydrochalcone glucosides of the extract were further separated in a second dimension centrifugal partition chromatography with the solvent system ethyl acetate/1,2-dimethoxyethane/water (2:1:1) in the descending mode with high purities (88.9-98.8%).


Assuntos
Balanophoraceae/química , Taninos Hidrolisáveis/isolamento & purificação , Extratos Vegetais/isolamento & purificação , Centrifugação , Distribuição Contracorrente , Taninos Hidrolisáveis/química , Medicinas Tradicionais Africanas , Extratos Vegetais/química
9.
Nat Prod Res ; 34(20): 2964-2970, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31009244

RESUMO

Three new muurolane type sesquiterpene glycosides, named balanoindicosides A - C (1 - 3), were isolated from the whole plant of Balanophora fungosa subsp. indica using various chromatographic methods. Their structures were determined by extensive analysis of HR-ESI-MS and NMR spectroscopy. The stereochemistry of muurolane sesquiterpene backbone was demonstrated by NOESY analysis. Configuration of C-12 in compounds 2 and 3 could be distinguished by chemical shift value of C-14. Compounds 1-3 exhibited weak cytotoxic activity towards HepG-2, HL-60, LU-1, and MCF-7 cell lines.


Assuntos
Balanophoraceae/química , Glicosídeos/química , Sesquiterpenos/química , Sesquiterpenos/farmacologia , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Ensaios de Seleção de Medicamentos Antitumorais , Glicosídeos/farmacologia , Células HL-60 , Células Hep G2 , Humanos , Células MCF-7 , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Sesquiterpenos Policíclicos/química , Espectrometria de Massas por Ionização por Electrospray
10.
Planta Med ; 85(11-12): 911-916, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30901776

RESUMO

Thonnigia sanguinea is a plant widely used in traditional African medicine against a variety of diseases. The obligate parasite is growing throughout tropical African forests and utilizes a large variety of hosts. Dihydrochalcone glucoside derivatives isolated from the subaerial parts of this plant were identified as potential antidiabetic lead compounds. In this study, an ultrahigh-performance liquid chromatographic method coupled with a photodiode array detector was developed for the quantitation of six major dihydrochalcone derivatives. The analytes were baseline separated in complex samples within 14 minutes on a Phenomenex Luna Omega 1.6 µm C18 column using a mobile phase consisting of water and acetonitrile (each + 0.01% trifluoroacetic acid) in gradient elution. Method validation confirmed the selectivity, linearity (R2 ≥ 0.9992), precision (inter-day ≤ 1.98%, intraday ≤ 2.00%), and accuracy (recovery rates of 97.4 - 106.3% for all analytes). At 280 nm, the LODs and LOQs were found to be lower than 1.42 and 4.30 µg/mL, respectively. Eight plant batches from the northern Angolan province of Uíge (collected in the wild or bought on markets) were extracted with methanol using an ultrasound-assisted extraction protocol and subsequently analyzed with the validated method. Results indicated high contents of dihydrochalcone glucosides in all eight samples. Most notably, the two bioactive constituents thonningianin A and B were present in fairly large amounts (2.42 - 5.35 w%).


Assuntos
Balanophoraceae/química , Chalconas/análise , Cromatografia Líquida de Alta Pressão/métodos , Glucosídeos/análise , Dispositivos Lab-On-A-Chip , Procedimentos Analíticos em Microchip/métodos
11.
J Nat Prod ; 81(9): 2091-2100, 2018 09 28.
Artigo em Inglês | MEDLINE | ID: mdl-30207720

RESUMO

Six new and four known dihydrochalcone glucoside derivatives (1-10), the phenylpropanoid coniferin (11), and the lignans (+)-pinoresinol (12) and lariciresinol (13) were isolated from the subaerial plant parts of Thonningia sanguinea in the course of a screening campaign for new antidiabetic lead compounds. The structures of the new substances were elucidated by HRESIMS, NMR, GC-MS, and ECD data evaluation. 2'- O-(3-Galloyl-4,6- O- Sa-hexahydroxydiphenoyl-ß-d-glucopyranosyl)-3-hydroxyphloretin (4), 2'- O-(4,6- O- Sa-hexahydroxydiphenoyl-ß-d-glucopyranosyl)phloretin (5), 2'- O-(3- O-galloyl-4,6- O- Sa-hexahydroxydiphenoyl-ß-d-glucopyranosyl)phloretin (6), and thonningianin B (9) showed moderate protein tyrosine phosphatase-1B inhibition in an enzyme assay (IC50 values ranging from 19 to 25 µM), whereas thonningianin A (10) was identified as a more potent inhibitor (IC50 = 4.4 µM). The observed activity differences could be explained by molecular docking experiments. The activity of 10 could further be confirmed in HEPG2 liver carcinoma cells, where the compound was able to increase the level of phosphorylated insulin receptors in a concentration-dependent manner.


Assuntos
Balanophoraceae/química , Chalconas/isolamento & purificação , Glucosídeos/isolamento & purificação , Proteína Tirosina Fosfatase não Receptora Tipo 1/antagonistas & inibidores , Chalconas/química , Chalconas/farmacologia , Cromatografia Gasosa-Espectrometria de Massas , Glucosídeos/química , Glucosídeos/farmacologia , Células Hep G2 , Humanos , Espectroscopia de Ressonância Magnética , Simulação de Acoplamento Molecular , Extratos Vegetais/farmacologia
12.
Fitoterapia ; 121: 129-135, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28694126

RESUMO

The bioactive substance investigation of Balanophora involucrata obtained 15 phenolic acids, including 5 new compounds (1-3, 8, 9), which were determined by various spectroscopic data analyses. Most isolated compounds displayed inhibitory effects on α-Glucosidase in vitro. For the potential inhibitors 8 (1.95µM) and 10 (9.02µM), the inhibition kinetics have been studied, which gave the Ki values as 0.68, 3.15µM respectively. And, in silico docking analyses have been performed to investigate the inhibitory mechanism of compounds 8 and 10. Additionally, most isolated compounds showed anti-oxidant activities in the DPPH scavenging assay. New compound 8 also could inhibit the acetyl transfer activity of GlmU moderately with the IC50 value of 18.21µM, which was a new antibacterial target.


Assuntos
Balanophoraceae/química , Sequestradores de Radicais Livres/química , Inibidores de Glicosídeo Hidrolases/química , Hidroxibenzoatos/química , Sequestradores de Radicais Livres/isolamento & purificação , Inibidores de Glicosídeo Hidrolases/isolamento & purificação , Hidroxibenzoatos/isolamento & purificação , Simulação de Acoplamento Molecular , Extratos Vegetais/química
13.
Chem Biodivers ; 10(6): 1081-7, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23776023

RESUMO

Balanophora involucrata HOOK.f. & THOMSON (Balanophoraceae) is a parasite plant often growing on the roots of leguminous plants. The whole herb has been used medicinally for the treatment of irregular menstruation, cough, hemoptysis, traumatic injury and bleeding, dizziness and gastralgia in Yunnan Province, China. The 2,2-diphenyl-2-picrylhydrazyl (DPPH) assay on the 60% aq. acetone extract of the fresh whole plant of B. involucrata showed considerable radical-scavenging activity (SC50 15.3 µg/ml). Further purification on the extract led to the isolation of one new phenolic glycoside, sieboldin-3'-ketocarboxylic acid (1), and one new cyanogenic glycoside, proacacipetalin 6'-O-ß-D-glucopyranoside (2), together with 26 known compounds including three 4"-O-galloyl and 2",3"-O-(S)-hexahydroxydiphenoyl (HHDP) derivatives of dihydrochalcone glucosides, seven hydrolyzable tannins, and alkane glycosides. The cyanogenic compound isolated from the Balanophoraceae family for the first time might be a signal molecule between B. involucrata and its hosts. The free-radical-scavenging activity of the isolated compounds was also examined by DPPH assay.


Assuntos
Balanophoraceae/química , Sequestradores de Radicais Livres/química , Glicosídeos/química , Fenóis/química , Sequestradores de Radicais Livres/isolamento & purificação , Glicosídeos/isolamento & purificação , Raízes de Plantas/química
14.
Fitoterapia ; 83(8): 1386-90, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22909424

RESUMO

One new flavanone (S)-5,7,3',5'-tetrahydroxy-flavanone-7-O-(6″-galloyl)-ß-D-glucopyranose (1), together with one known flavanone and four known dihydrochalcones were isolated from the ethyl acetate partitions from an alcoholic extract of the whole plant of Balanophora involucrata Hook. f. All of the compounds were characterized by spectroscopic methods. Their in vitro BACE inhibitory effects were evaluated. Compounds 1, 2, 4, and 5 were found to be a little more active than the positive control, although all of which are below 50% inhibition at 10µM.


Assuntos
Balanophoraceae/química , Flavanonas/farmacologia , Chalconas/química , Flavanonas/química , Estrutura Molecular
15.
Nat Prod Commun ; 7(1): 33-4, 2012 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22428237

RESUMO

A bioassay-oriented study was carried-out in order to validate the traditional uses of Lophophytum leandri, a parasitic plant used as a diuretic in traditional medical practices of Argentina. Four known flavonoids have been isolated from the active fraction. Quercetin-3-O-beta-D-glucopyranoside was identified as the active principle of the fraction. However, the diuretic activity of the extract and of the most active fraction had greater activity than that of the pure isolated compounds.


Assuntos
Balanophoraceae , Diuréticos/farmacologia , Extratos Vegetais/farmacologia , Animais , Balanophoraceae/química , Masculino , Ratos , Ratos Wistar
16.
Nan Fang Yi Ke Da Xue Xue Bao ; 31(10): 1641-8, 2011 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22027761

RESUMO

OBJECTIVE: MicroRNAs (miRNAs) play important roles in cell proliferation, differentiation and apoptosis. 1, 3, 4-tri-O-galloyl-6-O-caffeoyl-ß-D-glucopyranose (BJA32515) is a new natural ellagitannin compound extracted from Balanophora Japonica MAKINO. The effect of BJA32515 on the expression of miRNAs in cancer cells has not yet been explored. Objective The present study was carried out to examine the changes in miRNA expression profiles in human HepG(2) hepatocarcinoma cells following BJA32515 exposure. METHODS: The proliferation of BJA32515-exposed HepG(2) cells was assessed using a colorimetric assay (cell counting kit-8). The miRNA expression profile of the cancer cells was analyzed using a miRNA array and quantitative real-time PCR. Apoptosis was assessed by annexin V and propidium iodide staining. RESULTS: BJA32515 inhibited the cell proliferation and increased apoptosis in HepG(2) cancer cells. The exposure to BJA32515 also caused alterations in the miRNA expression profile in the cells, with 33 miRNAs upregulated and 59 down-regulated. The up-regulation of let-7a and miR-29a and the down-regulation of miR-373 and miR-197 were verified by quantitative real-time PCR. CONCLSION: BJA32515-modifed miRNA expression may mediate the antiproliferative effect of this compound in HepG(2) cancer cells.


Assuntos
Apoptose/efeitos dos fármacos , Balanophoraceae/química , Ácidos Cafeicos/farmacologia , Glucosídeos/farmacologia , Taninos Hidrolisáveis/farmacologia , MicroRNAs/metabolismo , Antineoplásicos/farmacologia , Ácidos Cafeicos/isolamento & purificação , Proliferação de Células , Regulação Neoplásica da Expressão Gênica , Glucosídeos/isolamento & purificação , Células Hep G2 , Humanos , Taninos Hidrolisáveis/isolamento & purificação , MicroRNAs/genética , Polifenóis
17.
J Agric Food Chem ; 59(4): 1109-14, 2011 Feb 23.
Artigo em Inglês | MEDLINE | ID: mdl-21265535

RESUMO

Tyrosinase, trypsin, and tryptase are known to play important roles in melanin production of human skin. This paper describes the study of the inhibitory effect of Balanophora fungosa on melanin. The 50% EtOH extract obtained from B. fungosa indicated an inhibitory effect on mushroom tyrosinase activity with an IC(50) value of 15 µg/mL. Bioassay-guided fractionation of the active extract resulted in the isolation of four known compounds. Their structures were identified as 1-O-(E)-caffeoyl-3-O-galloyl-4,6-(S)-HHDP-ß-d-glucopyranose (1), 1-O-(E)-caffeoyl-3,4,6-tri-O-galloyl-ß-d-glucopyranose (2), caffeoyl-ß-d-glucopyranose (3), and abietin (4) on the basis of spectroscopic analyses and comparison of their spectral data with those in the literature. Compounds 1 and 2 prevented pigmentation of melanin in a three-dimensional cultured human skin model. Furthermore, compounds 1 and 2 indicated inhibitory activities against trypsin and tryptase.


Assuntos
Balanophoraceae/química , Melaninas/antagonistas & inibidores , Melaninas/biossíntese , Extratos Vegetais/farmacologia , Linhagem Celular Tumoral , Inibidores Enzimáticos/farmacologia , Humanos , Melanócitos/metabolismo , Melanoma , Modelos Biológicos , Monofenol Mono-Oxigenase/antagonistas & inibidores , Extratos Vegetais/química , Pele , Inibidores da Tripsina/farmacologia , Triptases/antagonistas & inibidores
18.
J Plant Res ; 124(2): 311-4, 2011 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20927638

RESUMO

The high polysaccharide content of some plant species hinders the successful isolation of their DNA. As an alternative to the macro-extraction methods previously published for polysaccharide-rich plants, we present two techniques (STE/CTAB and HEPES/CTAB), which are performed in microcentrifuge tubes. These protocols are suitable for small amounts of silica gel-preserved plant tissue such as are commonly available from endangered plants. The critical step to remove polysaccharides was performing initial washes in either STE (0.25 M sucrose, 0.03 M Tris, 0.05 M EDTA) or HEPES (2% ß-mercaptoethanol, 0.2% PVP, 0.1 M HEPES, pH 8.0) buffer. Precipitating the DNA at room temperature with isopropanol also aided in decreasing polysaccharide co-precipitation. Of the two protocols we present the STE/CTAB method has the advantages of being more cost-effective and avoiding the use of the hazardous chemical ß-mercaptoethanol.


Assuntos
DNA de Plantas/isolamento & purificação , Plantas/química , Polissacarídeos/metabolismo , Balanophoraceae/química , Balanophoraceae/genética , Soluções Tampão , Cetrimônio , Compostos de Cetrimônio/química , Técnicas Genéticas , Malvaceae/química , Malvaceae/genética , Folhas de Planta/química , Folhas de Planta/genética , Plantas/genética , Sílica Gel , Tensoativos/química
19.
Planta Med ; 77(5): 477-81, 2011 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20979022

RESUMO

Five new phenylpropanoids, named balajaponins A-E (1-5), were isolated from Balanophora japonica, along with 24 known compounds. Among them, three hydrolysable tannins (6-8) showed specific in vitro α-glucosidase inhibition, with IC50 values in the range of 1-4 µM. Kinetic analysis revealed that they all acted in a noncompetitive mode.


Assuntos
Balanophoraceae/química , Inibidores de Glicosídeo Hidrolases , Taninos Hidrolisáveis/farmacologia , Fenilpropionatos/farmacologia , Inibidores Enzimáticos/química , Inibidores Enzimáticos/farmacologia , Taninos Hidrolisáveis/química , Concentração Inibidora 50 , Cinética , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Fenilpropionatos/química , Extratos Vegetais/química , Extratos Vegetais/farmacologia , alfa-Glucosidases/metabolismo
20.
Zhong Yao Cai ; 34(11): 1734-40, 2011 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-22506400

RESUMO

OBJECTIVE: To study the effect of 1,2,6-Tri-O-galloyl-beta-D-glucopyranose (BJA32531) on the miRNA expression during BJA32531-induced cytotoxicity in human HepG2 hepatocarcinoma cells. METHODS: Cell proliferation was assessed using a colorimetric assay (cell counting kit-8). Apoptosis was assessed by annexin V and propidium iodide staining. The miRNA expression profile of the cancer cells was analyzed by a miRNA array and quantitative real-time PCR. RESULTS: BJA32531 inhibited the cell proliferation and increased apoptosis in HepG2 cancer cells. Cellular exposure to BJA32531 influenced the miRNA expression pattern in the cells, including 19 upregulated and 85 down-regulated miRNAs in the cells. The up-regulations of let-7a and miR-10b as well as the down-regulations of miR-132 and miR-125b were verified to be consistent with the the results of the miRNA array. CONCLUSION: Our study suggests that the mechanisms by which BJA32531 exerted the antiproliferative effects on HepG2 cancer cells may be related to its regulation of miRNA.


Assuntos
Antineoplásicos/farmacologia , Proliferação de Células/efeitos dos fármacos , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , MicroRNAs/metabolismo , Polifenóis/farmacologia , Apoptose/efeitos dos fármacos , Balanophoraceae/química , Carcinoma Hepatocelular/metabolismo , Carcinoma Hepatocelular/patologia , Relação Dose-Resposta a Droga , Citometria de Fluxo , Células Hep G2 , Humanos , Neoplasias Hepáticas/metabolismo , Neoplasias Hepáticas/patologia , MicroRNAs/genética , Estrutura Molecular , Análise de Sequência com Séries de Oligonucleotídeos , Polifenóis/química , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Fatores de Tempo
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