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1.
Korean J Parasitol ; 56(6): 603-607, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30630282

RESUMO

This study was carried out to determine the pathogen-causing diarrhoea in sheep Ovis aries in the Qinghai Tibetan Plateau Area, China. A trophozoite was identified as species of ciliate alveolates infecting the sheep based on morphological characteristics examined by microscope. It was mostly spherical, colourless and transparent, with many vesicles. Macronucleus and contractile vacuoles could not be distinguished. Size of the trophozoite was 80-180 × 70-150 µm and its surface was covered with cilia. Molecular analysis based on sequences of 18S rRNA and ITS genes confirmed the ciliate species as Balantidium coli. According to the literature, there have been many epidemiological investigations of B. coli infection in pigs, monkeys and humans. To our knowledge, this was the first report of B. coli infections in sheep in the Qinghai Tibetan Plateau Area of China, or eleswhere around the world. Importantly, the sheep case was rare but raised our concern that B. coli may spread across species and expand its host range.


Assuntos
Balantidíase/veterinária , Balantidium/isolamento & purificação , Diarreia/veterinária , Doenças dos Ovinos/parasitologia , Animais , Balantidíase/parasitologia , Balantidium/classificação , Balantidium/citologia , Balantidium/genética , Análise por Conglomerados , DNA de Protozoário/química , DNA de Protozoário/genética , DNA Ribossômico/química , DNA Ribossômico/genética , DNA Espaçador Ribossômico/química , DNA Espaçador Ribossômico/genética , Diarreia/parasitologia , Masculino , Microscopia , Filogenia , RNA Ribossômico 18S/genética , Análise de Sequência de DNA , Ovinos , Carneiro Doméstico , Tibet
2.
Vet Parasitol ; 210(3-4): 240-5, 2015 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-25920329

RESUMO

Balantidium coli is a protozoa that can determine dysentery in humans, pigs and non-human primates having zoonotic potential. The lack of standardization in isolation and maintenance hinders the development of research on its biology and epidemiology. This study is aimed to standardize the isolation and maintenance of this parasite from animal feces, in culture medium, Pavlova modified. From 2012 to 2014, 1905 fecal samples were collected from captive animals of Rio de Janeiro. Were selected for isolation samples with a minimum of 10 trophozoites and/or 30 cysts of B. coli, totaling 88 pigs, 26 Cynomolgus and 90 rhesus macaques. In the presence of cysts, the sample was homogenized in saline solution, 500 µL was removed and inoculated into culture medium. The material that contained trophozoites the inoculum was made from 240 µL of fecal solution. All inoculate tubes with the subcultures were kept at 36°C, and sterile rice starch was always added to the medium. The parasites isolate from pigs, 34%, and from Cynomolgus 38.4% were maintained in vitro for a period of more than 24 months. These procedures proved to be adequate for isolation and maintenance of B. coli from different animals, they were found to be inexpensive and easy to perform.


Assuntos
Doenças dos Animais/parasitologia , Balantidíase/veterinária , Balantidium/isolamento & purificação , Primatas/parasitologia , Doenças dos Suínos/parasitologia , Animais , Balantidíase/parasitologia , Balantidium/citologia , Balantidium/crescimento & desenvolvimento , Brasil , Meios de Cultura , Fezes/parasitologia , Suínos
3.
Parazitologiia ; 48(5): 348-57, 2014.
Artigo em Russo | MEDLINE | ID: mdl-25929106

RESUMO

The structure of the ciliate Balantidium helenae Bezzenberger, 1904 was investigated with the use of optical and electron microscopy. It was demonstrated that, together with the basic dimensional characteristics, main distinguishing features of the studied species include the following characters: the presence of a pointed prominence on the posterior margin of the cell; the presence of elongated cilia on the left margin of the vestibulum; and different shape and size of cortical ridges. Two morphologically different types of electron-dense bodies (supposedly hydrogenosoms) and also endobiotic bacteria were also found in the cytoplasm of B. helenae.


Assuntos
Balantidium/citologia , Balantidium/ultraestrutura , Intestinos/parasitologia , Rana temporaria/parasitologia , Animais , Cílios/ultraestrutura , Microscopia/métodos , Microscopia Eletrônica
4.
Parasitol Res ; 106(1): 177-82, 2009 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19865830

RESUMO

Redescription of Balantidium polyvacuolum Li 1963, collected from the hindgut of Xenocypris davidi and Xenocypris argentea, from Niushan Lake Fishery (30 degrees 19' N, 114 degrees 31' E) in Wuhan City, Hubei Province, China in April and June 2007 is presented in this paper to complete Li's description at both light and scanning electronic microscopic levels. The unique body shape of B. polyvacuolum-highly arched dorsal side and flattened ventral surface-as well as its remarkable concave platelet present in the centroventral were well described and compared with other close Balantidium species. Besides, two types of vestibulum shape are observed in our present work, which may suggest the existence of two subspecies or genotype species of these balantidia.


Assuntos
Balantidíase/veterinária , Balantidium/citologia , Cyprinidae/parasitologia , Intestinos/parasitologia , Animais , Balantidíase/parasitologia , Balantidium/isolamento & purificação , China , Microscopia , Microscopia Eletrônica de Varredura
5.
Wiad Parazytol ; 42(2): 159-69, 1996.
Artigo em Polonês | MEDLINE | ID: mdl-8967075

RESUMO

In trophozoites and cysts of Balantidium coli the contents of nucleic acids were compared, with the use of cytochemical methods. There is more RNA (nuclear and cytoplasmatic) in trophozoites, but the content of DNA is the same in both trophozoites and cysts. Some morphometric parameters, allowing to compare trophozoites and cysts of B. coli, were obtained on the basis of cytophotometric determination of the cytochemical reactions' intensity and its computer analysis. These studies showed greater compactness of nuclear chromatin, higher homogeneity of chromatin's structures in cysts in comparison with trophozoites, and finally, decrease in the circumference and area of cysts of B. coli.


Assuntos
Balantidium/citologia , Animais , Balantidium/fisiologia , Cromatina/química , Citofotometria , DNA de Protozoário/análise , Histocitoquímica , RNA de Protozoário/análise , Esporos/citologia
6.
Acta Cytol ; 21(1): 123-4, 1977.
Artigo em Inglês | MEDLINE | ID: mdl-264743

RESUMO

Balantidium coli were detached in a routine cytologic smear of ascitic fluid. The morphologic features were clearly discernible in the Papanicolaou stained smears.


Assuntos
Balantidíase/diagnóstico , Peritonite/diagnóstico , Balantidíase/microbiologia , Balantidium/citologia , Feminino , Humanos , Pessoa de Meia-Idade , Peritonite/microbiologia
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