RESUMO
This study analyzed the effect of pH (X1), temperature (X2) and inulin amount (X3) on transformation of isoflavones (daidzin and daidzein) to equol in soymilk fermented with Bifidobacterium spp. All responses significantly (pâ¯<â¯0.05) fitted into quadratic models with coefficients of determination (R2) close to 1 (0.935-0.989). At 24â¯h of fermentation, amounts of daidzin and daidzein were influenced by all factors. While at 48â¯h, all factors affected daidzin and only temperature affected daidzein. Equol production was influenced by pH and temperature in 24â¯h and by all factors in 48â¯h fermentation. The optimum conditions for equol production were pH 8, 30⯰C and 0.5% inulin. Model validation demonstrated there was no significant (pâ¯>â¯0.05) difference between the experimental and predicted values, suggested the suitability of established models in explaining the daidzin and daidzein transformation to equol as a function of pH, temperature and inulin.
Assuntos
Bifidobacterium breve/metabolismo , Bifidobacterium longum/metabolismo , Equol/biossíntese , Microbiologia de Alimentos/métodos , Leite de Soja/metabolismo , Bifidobacterium breve/citologia , Bifidobacterium longum/citologia , Fermentação , Humanos , Concentração de Íons de Hidrogênio , Inulina/metabolismo , Isoflavonas/metabolismo , Reprodutibilidade dos Testes , TemperaturaRESUMO
In addition to functioning as detergents that aid digestion of dietary lipids in the intestine, some bile acids have been shown to exhibit antimicrobial activity. However, detailed information on the bactericidal activities of the diverse molecular species of bile acid in humans and rodents is largely unknown. Here, we investigated the toxicity of 14 typical human and rodent free bile acids (FBAs) by monitoring intracellular pH, membrane integrity, and viability of a human intestinal bacterium, Bifidobacterium breve Japan Collection of Microorganisms (JCM) 1192T, upon exposure to these FBAs. Of all FBAs evaluated, deoxycholic acid (DCA) and chenodeoxycholic acid displayed the highest toxicities. Nine FBAs common to humans and rodents demonstrated that α-hydroxy-type bile acids are more toxic than their oxo-derivatives and ß-hydroxy-type epimers. In five rodent-specific FBAs, ß-muricholic acid and hyodeoxycholic acid showed comparable toxicities at a level close to DCA. Similar trends were observed for the membrane-damaging effects and bactericidal activities to Blautia coccoides JCM 1395T and Bacteroides thetaiotaomicron DSM 2079T, commonly represented in the human and rodent gut microbiota. These findings will help us to determine the fundamental properties of FBAs and better understand the role of FBAs in the regulation of gut microbiota composition.