RESUMO
The pattern of keratin expression in hamster cheek pouch epithelium during 15-wk of DMBA-induced carcinogenesis was studied. The sequential changes in cytokeratins of premalignant and malignant tissues and comparative investigation of normal epithelial tissues were examined during a weekly sequential DMBA-induced chemical carcinogenesis. Keratin polypeptides of normal pouch epithelium appear in a molecular weight range of 43-67 kd and 5-6 proteins can be identified. The disappearance of high molecular weight keratin (61-67 kd) was observed from the 6-wk DMBA-treated premalignant group to the 15-wk DMBA-treated malignant group. An additional keratin polypeptide was noted initially on the 11th-wk-DMBA-treated group and remained to the 15th-wk-DMBA treated group.
Assuntos
Queratinas/análise , Mucosa Bucal/análise , Neoplasias Bucais/análise , Lesões Pré-Cancerosas/análise , 9,10-Dimetil-1,2-benzantraceno , Animais , Bochecha/análise , Bochecha/patologia , Cricetinae , Eletroforese em Gel de Poliacrilamida , Epitélio/análise , Epitélio/patologia , Immunoblotting , Queratinas/classificação , Masculino , Mesocricetus , Mucosa Bucal/patologia , Neoplasias Bucais/patologia , Palato/análise , Lesões Pré-Cancerosas/patologia , Pele/análise , Dodecilsulfato de SódioRESUMO
Vitamin A levels in tissues of 20 normal adult hamsters on a standard diet were measured colorimetrically. No significant difference between male and female animals was found for any of the tissues sampled. The mean vitamin A value for blood plasma in 20 animals was 53.4 micrograms/dl. Mean values for liver, kidneys, flank skin and cheek pouch were 813, 1.29, 1.84 and 1.31 mg/g wet weight, respectively. The vitamin assay was less suitable for small organs such as trachea.
