Unraveling the molecular response of Brassica napus hairy roots in the active Naphthol blue-black removal: Insights from proteomic analysis.

In vitro plant cultures are able to remove and metabolise xenobiotics, making them promising tools for decontamination strategies. In this work, we evaluated Brassica napus hairy roots (HRs) to tolerate and remove high concentrations of the azo dye Naphthol Blue-Black (NBB). Experiments were performed using both growing and resting culture systems at different pHs. Reuse of HRs biomass was evaluated in successive decolourisation cycles. Proteomics was applied to understand the molecular responses likely to be involved in the tolerance and removal of NBB. The HRs tolerated up to 480 µg mL-1 NBB, and 100 % removal was achieved at 180 µg mL-1 NBB after 10 days using both culture systems. Interestingly, the HRs are robust enough to be reused, showing 55-60 % removal even after three reuse cycles. The highest dye removal rates were achieved during the first 2 days of incubation, as initial removal is mainly driven by passive processes. Active mechanisms are triggered later by regulating the expression of proteins with different biological functions, mainly those related to xenobiotic metabolism, such as hydrolytic and redox enzymes. These results suggest that B. napus HRs are a robust tool that could make a significant contribution to textile wastewater treatment.

The Co-occurrence Matrix and the Correlation Network of Phytophagous Insects Are Driven by Abiotic and Biotic Variables: the Case of Canola.

Co-occurrence a correlation profiles are driven by different factors (exogenous and endogenous) and drawing a profile of association between species based on co-occurrence, without assessing how these species vary in terms of ecological niche can lead to wrong conclusions. The objective was to determine the co-occurrence and correlation patterns of phytophagous insects in canola crop and to evaluate how these patterns varied according to the crop stage (phenology-biotic) and sowing times (agricultural practice-abiotic). We found that the patterns of co-occurrence and correlation between species were reflections of population variations due to the phenology and sowing times of canola. Variations in the multi-species abundance matrix were influenced by mean air temperature and accumulated rainfall. The main species associated with canola in southern Brazil, in terms of abundance, were P. xylostella, D. speciosa, and N. viridula. These species were mostly negatively associated. When evaluating their population variations, we found that they explore different temporal niches, whether in terms of phenology or sowing times. Finally, we demonstrate empirically that despite being important, association patterns based on co-occurrence and correlation should be interpreted in light of the understanding of patterns of niche exploitation and temporal variation of species.

A model-based evaluation of farmers' income variability under climate change (case study: autumn crops in Iran) / Uma avaliação baseada em modelo da variabilidade da renda dos agricultores sob mudança climática (estudo de caso: culturas de outono no Irã)

The study strives to analyze the potential variations of farmers' income under climate change by using Ricardian approach. The case study was Mazandaran province of Iran and three autumn crops, i.e. wheat, barley and canola were considered as the investigated crops. The Long Ashton Research Station Weather Generator (LARS-WG) model was selected to downscale the climate data. Three climate variables were downscaled for the years 2020-2080 under three climate scenarios: optimistic (RCP2.6), medium (RCP4.5), and pessimistic (RCP8.5). The Ricardian approach was also employed to predict the economics of climate change. Accordingly, the mean monthly temperature of the province is projected to have an upward trend under all climate scenarios, however, the rainfall pattern would be varied. The results of economic impacts of climate change also approved that the net income of investigated crops would be different trends under climate change scenarios. Accordingly, the variations of air temperature and rainfall would lead that the net income increases for wheat and barley, while it decreases for canola.

O estudo se esforça para analisar as variações potenciais da renda dos agricultores sob a mudança climática usando a abordagem Ricardiana. O estudo de caso foi a província de Mazandaran do Irã e três culturas de outono, ou seja, trigo, cevada e canola foram consideradas como as culturas investigadas. O modelo Long Ashton Research Station Weather Generator (LARS-WG) foi selecionado para reduzir a escala dos dados climáticos. Três variáveis ​​climáticas foram reduzidas para os anos 2020-2080 em três cenários climáticos: otimista (RCP2.6), médio (RCP4.5) e pessimista (RCP8.5). A abordagem ricardiana também foi empregada para prever a economia das mudanças climáticas. Assim, projeta-se que a temperatura média mensal da província tenha uma tendência ascendente em todos os cenários climáticos, no entanto, o padrão de chuvas seria variado. Os resultados dos impactos econômicos das mudanças climáticas também aprovaram que a renda líquida das lavouras investigadas seria de tendências diferentes em cenários de mudanças climáticas. Nesse sentido, as variações da temperatura do ar e da pluviosidade fariam com que a renda líquida aumentasse para o trigo e a cevada, enquanto diminuía para a canola.

Transcriptome and Physiological Analysis of Rapeseed Tolerance to Post-Flowering Temperature Increase.

Climate-change-induced temperature fluctuations pose a significant threat to crop production, particularly in the Southern Hemisphere. This study investigates the transcriptome and physiological responses of rapeseed to post-flowering temperature increases, providing valuable insights into the molecular mechanisms underlying rapeseed tolerance to heat stress. Two rapeseed genotypes, Lumen and Solar, were assessed under control and heat stress conditions in field experiments conducted in Valdivia, Chile. Results showed that seed yield and seed number were negatively affected by heat stress, with genotype-specific responses. Lumen exhibited an average of 9.3% seed yield reduction, whereas Solar showed a 28.7% reduction. RNA-seq analysis of siliques and seeds revealed tissue-specific responses to heat stress, with siliques being more sensitive to temperature stress. Hierarchical clustering analysis identified distinct gene clusters reflecting different aspects of heat stress adaptation in siliques, with a role for protein folding in maintaining silique development and seed quality under high-temperature conditions. In seeds, three distinct patterns of heat-responsive gene expression were observed, with genes involved in protein folding and response to heat showing genotype-specific expression. Gene coexpression network analysis revealed major modules for rapeseed yield and quality, as well as the trade-off between seed number and seed weight. Overall, this study contributes to understanding the molecular mechanisms underlying rapeseed tolerance to heat stress and can inform crop improvement strategies targeting yield optimization under changing environmental conditions.

High-power ultrasound bleaching technique for canola oil (Brassica napus L.): Pigments removal and quality parameters.

Canola seeds (Brassica napus L.) are among the most commonly used seeds in Mexico for vegetable oil production. This is based on the high yield and content of polyunsaturated and monounsaturated fatty acids. During oil bleaching, it is important to maintain fatty acids in their cis configuration because of the health concerns associated with trans-fatty acid consumption. In this sense, the industrial processing parameters employed for this purpose present some limitations, such as high temperatures and long times, which may change the cis configuration to trans. In addition, the amount of bleaching clay employed for this process could be a source of contamination because it is disposed of after treatment. Therefore, the aim of this study was to develop a bleaching process for canola oil using high-power ultrasound (US). US processing was applied to nine treatments with different processing times (60, 75, and 90 min), clay percentages (1, 2, and 3%), and temperatures (60 and 80 °C) to determine the concentrations of chlorophyll a and b (µg chlo/100 g oil), carotenes (µg ß-carotene/100 g oil), color (L*, a*, b*, C*, and h°), iodine value (g I2/100 g oil), and finally carrying out a spectroscopic analysis (ATR-FTIR and Raman). A conventional bleaching treatment (100 °C for 180 min, 3% bleaching clay) was used as a control. The results revealed that US treatments with 2% clay at 60 °C for 60 and 90 min eliminated most of the chlorophyll compounds (98%). However, in terms of carotenes reduction, these identical treatments exhibited a similar tendency to that of the control (approximately 30% decrease). These findings also affected the sample color, in which US treatments revealed chromatic coordinates that indicated yellow tones with chroma values that were more intense than those in the control samples. In terms of the iodine value, such treatments fulfilled the international standards for vegetable oils (90-100 g I2/100 g oil). Finally, the spectroscopic study revealed no trans configurations or the presence of different chemical compounds after US treatment, because neither of them presented typical peaks for those molecular configurations. In this regard, US can be a useful methodology for bleaching vegetable oils, helping to reduce time, and bleaching clay with similar pigment reduction results.

Forecasting Brassica napus production under climate change with a mechanistic species distribution model.

Brassica napus, a versatile crop with significant socioeconomic importance, serves as a valuable source of nutrition for humans and animals while also being utilized in biodiesel production. The expansion potential of B. napus is profoundly influenced by climatic variations, yet there remains a scarcity of studies investigating the correlation between climatic factors and its distribution. This research employs CLIMEX to identify the current and future ecological niches of B. napus under the RCP 8.5 emission scenario, utilizing the Access 1.0 and CNRM-CM5 models for the time frame of 2040-2059. Additionally, a sensitivity analysis of parameters was conducted to determine the primary climatic factors affecting B. napus distribution and model responsiveness. The simulated outcomes demonstrate a satisfactory alignment with the known current distribution of B. napus, with 98% of occurrence records classified as having medium to high climatic suitability. However, the species displays high sensitivity to thermal parameters, thereby suggesting that temperature increases could trigger shifts in suitable and unsuitable areas for B. napus, impacting regions such as Canada, China, Brazil, and the United States.

Ruminal responses, digestibility, and blood parameters of beef cattle fed diets with different oilseeds.

It aimed to evaluate the effects of different whole oilseeds in lipid-rich diets on nutrient intake, apparent digestibility, ingestive behavior, and ruminal and blood parameters of steers. A control diet (without oilseed) and four diets containing whole oilseeds (cotton, canola, sunflower, and soybean) were tested. All diets used the whole-plant corn silage at 400 g/kg as roughage. Five diets, being a control diet (without oilseed) and four diets containing whole oilseeds (cotton, canola, sunflower, and soybean), were tested. All diets used the whole-plant corn silage at 400 g/kg as roughage. Five rumen fistulated crossbreed steers, in a 5 × 5 Latin square design were distributed using five periods of 21 days. The steers fed cottonseed and canola diets had lower dry matter intakes (6.6 kg/day). Steers showed higher averages of time in rumination for treatments with sunflower, soybean, and cottonseed (406, 362, and 361 min/day, respectively). There was no treatment effect for the ruminal pH and ammonia (NH3) variables. There was an effect of the treatment on the volatile fatty acid concentrations. The animals that received soybean showed a higher plasma urea concentration (50.7 mg/dL). Animals fed the control diet showed lower serum cholesterol levels (111.8 mg/dL) than those fed diets containing whole cottonseed, canola, sunflower, and soybean (152.7, 137.1, 146.9, and 138.2 mg/dL, respectively). We recommended using whole soybean or sunflower seeds to formulate lipid-rich diets with 70 g/kg of ether extract for crossbreed steers in the feedlot.

Seletividade e eficácia de herbicidas aplicados em canola para controle de plantas daninhas / Selectivity and efficacy of herbicides applied to canola for weed control

O uso de herbicidas para o controle de plantas daninhas é ométodo mais empregado pela eficácia, rapidez e menor custo, porém poucos são os produtos registrados à cultura da canola. Diante disso, objetivou-se com os trabalhos avaliar a seletividade (ensaio I) e a eficácia (ensaio II) de herbicidas aplicados de modo isolado ou associados em pré e pós-emergência da canola para o controle de plantas daninhas. Os experimentos foram instalados em delineamento de blocos casualizados, com quatro repetições. Em pré-emergência aplicou-se os herbicidas oxyfluorfen, pendimethalin, flumiozaxin, trifluralin e em pós-emergência o fluazifop-p-butil, mais as testemunhas capinada e infestada. Foram avaliadas a fitotoxicidade e as características fisiológicas no ensaio de seletividade, e o controle das plantas daninhas nabo, azevém eaveia preta no experimento de eficácia. Nos dois experimentos determinou-se os números de síliquas por planta, de grãos por síliqua, densidade de plantas, massa de mil grãos e a produtividade de grãos. O oxyfluorfen aplicado em isolado ou associado ao fluazifop-p-butil ocasionouas maiores fitotoxicidades ao híbrido de canola Diamond. As menores fitotoxicidades foram observadas para o pendimethalin e ao fluazifop-p-butil aplicados em isolado ou associados. Todos os herbicidas testados causaram estresse na concentração interna de CO2, transpiração, condutância estomática, atividade fotossintética, eficiência do uso da água e de carboxilação. O flumioxazin e o fluazifop-p-butil aplicados em pré e pós-emergência da canola apresentaram os melhores resultados para os componentes de rendimento de grãos da cultura, especialmente maior produtividade, juntamente com a testemunha capinada no ensaio de seletividade. Nenhum dos tratamentos com herbicidas controlaram adequadamente o nabo (experimento 2), e a presença desta planta daninha afetounegativamente os componentes de rendimento de grãos da canola. O fluazifop-p-butil aplicado em isolado ou associado ao oxifluorfen, pendimethalin, flumioxazin e trifluralin apresentaram os melhores controles de azevém e aveia preta.(AU)

The use of herbicides for weed control is the most used method due to its effectiveness, speed, and lower cost, but few products are registered for canola crops. In this way, the objective of this work was to evaluate the selectivity (experiment 1) and efficiency(experiment 2) of herbicides applied alone or in association in pre-and post-emergence of canola for weed control. The experiments were carried out in randomized blocks design, with four replications. In pre-emergence, the herbicides oxyfluorfen, pendimethalin, flumiozaxin, and trifluralin were applied, and in post-emergence, fluazifop-p-butyl, plus weeded and infested controls. Phytotoxicity and physiological characteristics were evaluated in the selectivity experiment, and the control of turnip, ryegrass, and black oat weeds were evaluated in the efficacy experiment. In both experiments, the number of siliques per plant, grains per silique, plant density, thousand-grain weight, and grain yield were determined. Oxyfluorfen applied alone or associated with fluazifop-p-butyl caused the highest phytotoxicity to the Diamond canola hybrid. The lowest phytotoxicities were observed for pendimethalin and fluazifop-p-butyl applied alone or in an association. All tested herbicides caused stress on internal CO2concentration, transpiration, stomatal conductance, photosynthetic activity, water use efficiency, and carboxylation efficiency. Flumioxazin and fluazifop-p-butyl applied pre-and post-emergence of canola showed the best results for the crop yield components, especially higher productivity, also with the weeded control in the selectivity experiment. None of the herbicide treatments controlled turnip properly (experiment 2), and the presence of this weed negatively affected canola yield components. Fluazifop-p-butyl applied alone or in association with oxyfluorfen, pendimethalin, flumioxazin, and trifluralin showed the best controls of ryegrass and black oat.(AU)

Ácido salicílico como atenuador do déficit hídrico em canola / alicylic acid as a water deficit mitigator in canola

A aplicação dereguladores de crescimento vegetal representa uma alternativa para atenuar a ocorrência de estresse nos cultivos, tais como períodos de escassez hídrica na canola (Brassica napusL.) Diante disso, objetivou-se avaliar a aplicação de ácido salicílico no desenvolvimento inicial da cultura da canola submetida a déficit hídrico. O experimento foi conduzido em casa de vegetação, no delineamento experimental de blocos casualizados em esquema fatorial 5 x 2, composto por cinco doses de ácido salicílico (0; 0,5; 1,0; 1,5 e 2,0 mM L-1) e dois regimes hídricos, com e sem déficit hídrico (25% e 80% da capacidade de vaso), com quatro repetições. As variáveis analisadas foram injúrias visuais, altura de plantas, teor de clorofila e massaseca da parte aérea. A aplicaçãode diferentes doses de ácido salicílico não apresentou diferenças significativas em relação a não aplicação. O déficit hídrico resultou em maior porcentagem de injúrias, menor altura e massa seca de plantas, indicando a ocorrência de estresse à cultura. Aaplicação de ácido salicílico não atenuou os efeitos do déficit hídrico, assim como não demonstrou efeitos benéficos no desenvolvimento das plantas de canola.(AU)

The application of plant growth regulators represents an alternative to attenuate the occurrence of stress in crops, such as periods of water scarcity in canola (Brassica napus L.). Therefore, the objective was to evaluate the application of salicylic acid in the initial development of the crop. of canola subjected to water deficit. The experiment was carried out in a greenhouse, in a randomized block design in a 5 x 2 factorial scheme, consisting of five doses of salicylic acid (0; 0.5; 1.0; 1.5 and 2.0 mM L-1) and two water regimes, with and without water deficit (25% and 80% of vessel capacity), with four replications. The variables analyzed were visual injuries, plant height, chlorophyll content and shoot dry mass. The application of different doses of salicylic acid did not present significant differences in relation to non-application. The water deficit resulted in a higher percentage of injuries, lower height and dry mass of plants, indicating the occurrence of stress to the crop. The application of salicylic acid did not attenuate the effects of water deficit, as well as it did not demonstrate beneficial effects on the development of canola plants.(AU)

Morphometric and molecular characterization of an unpigmented haemosporidian parasite in the Neotropical turnip-tailed gecko (Thecadactylus rapicauda).

Morphological traits from blood stages have been the gold standard for determining haemosporidian parasite species. However, the status of some taxa and the value of such traits in parasites from reptiles remain contentious. The scarce sampling of these species worsens the situation, and several taxa lack molecular data. A survey was performed in the Magdalena Department in Colombia, where 16 species of reptiles were captured. A peculiar haemosporidian parasite was found in the Turnip-tailed gecko Thecadactylus rapicauda. This haemosporidian does not show malarial pigment in blood stages under light microscopy; thus, it fits the Garnia genus's characters belonging to the Garniidae. However, the phylogenetic analyses using a partial sequence of cytochrome b and the mitochondrial DNA placed it within the Plasmodium clade. Our findings suggest that many putative Garnia species belong to the genus Plasmodium, like the one reported here. This study either shows that visible malarial pigment in blood stages is not a diagnostic trait of the genus Plasmodium or malarial pigment might be present in an undetectable form under a light microscope. In any case, the current taxonomy of haemosporidian parasites in reptiles requires revision. This study highlights the importance of using molecular and morphological traits to address taxonomic questions at the species and genus levels in haemosporidian parasites from reptiles.

Canola seed vigor under controlled deterioration testing / Vigor de sementes de canola pelo teste de deterioração controlada

Canola (Brassica napus L. var. oleifera) is an oilseed with considerable economic importance for human and animal consumption and biodiesel production. Vigor testing is essential in the search for greater advances in determining seed quality. In this respect, the present study aimed to adapt controlled deterioration testing to assess canola seed vigor. To that end, four seed lots of the Nuola 300 canola hybrid were initially characterized using the following tests: moisture content, thousand seed weight, germination first count, germination percentage, germination speed index, initial stand, seedling emergence percentage, emergence speed index, and cold testing. For controlled deterioration, the initial moisture content was adjusted to 15, 18, 20 and 22% and the samples were then placed in a water bath at 41 and 45 °C in a water bath for 24 hours, for germination and assessed five days after sowing. In canola seeds, controlled deterioration tests should be conducted at an 18% moisture content and temperature of 45 °C for 24 hours to evaluate physiological potential.(AU)

A canola (Brassica napus L. var. oleifera) é uma espécie oleaginosa de grande importância econômica para alimentação humana e animal e para produção de biodiesel. Na busca por maiores avanços na obtenção de sementes de qualidade, o uso de teste de vigor é imprescindível. Nesse sentido, o objetivo do trabalho foi adequar a metodologia do teste de deterioração controlada para avaliação do vigor de sementes de canola. Para tanto, quatro lotes de sementes de canola do híbrido Nuola 300 foram avaliados quanto à caracterização inicial dos lotes, pelos testes: determinação do grau de umidade, peso de mil sementes, primeira contagem de germinação, germinação, índice de velocidade de germinação, estande inicial, emergência, índice de velocidade de emergência e teste de frio. Para o teste de deterioração controlada, o grau de umidade inicial foi ajustado para 15%, 18%, 20% e 22% e, posteriormente, as amostras foram expostas às temperaturas de 41 °C e 45 °C, em banho-maria, durante 24 horas, postas para germinarem e avaliadas ao quinto dia após a semeadura. O teste de deterioração controlada em sementes de canola deve ser conduzido com o grau de umidade a 18%, sob a temperatura de 45 °C, durante 24 horas, para avaliação do potencial fisiológico.(AU)

Path analysis and near-infrared spectroscopy in canola crop / Análise de trilha e espectroscopia no infravermelho próximo na cultura da canola

This study measured the effect of the association between agronomic traits related to the yield of canola grains grown at different sowing dates through path analysis. Another objective was to obtain a method to predict the oil content in the grains, fitting a multivariate model through near-infrared (NIR) spectroscopy analysis. The experiment was conducted in the field using a randomized block design in plots subdivided by time, with four plots (sowing dates), six subplots (canola hybrids), and four replicates. In each hybrid, phenological observations were performed, and the grain yield was determined. The data were subjected to analysis of variance in the R environment using the F test at 5% probability. The oil content in the grains was determined by the traditional chemical method, and based on the NIR spectral signature of the grain samples, partial least squares regression (PLS-R) was established to estimate the oil content in the canola grains. The sowing dates influenced the production components and oil content of the grains of all hybrids. The trait number of grains in five plants (0.6857) and their height (0.4943) had greater estimates of positive correlations with grain yield, as well as higher values of positive direct effects on yield (0.2494 and 0.1595, respectively). The NIR technique combined with PLS-R was able to predict the oil content in the grains, resulting in good predictive models (R2 of 0.86 and root mean square error (RMSE) of 1.56 in external validation).

Objetivou-se mensurar o efeito da associação entre caracteres agronômicos relacionados à produtividade de grãos de canola cultivada em diferentes épocas de semeadura, através da análise de trilha. Assim como também objetivou-se obter um método para predizer o teor de óleo nos grãos, ajustando um modelo multivariado através da análise por espectroscopia na região do infravermelho próximo. O experimento foi conduzido em campo, utilizando-se o delineamento de blocos ao acaso, em parcelas subdivididas no tempo, sendo quatro parcelas (épocas de semeadura) e seis subparcelas (híbridos de canola), com quatro repetições. Em cada híbrido foram realizadas observações fenológicas e determinada a produtividade de grãos. Os dados foram submetidos à análise de variância em ambiente R pelo teste F, a cinco de probabilidade. O teor de óleo nos grãos foi determinado pelo método químico tradicional, e com base na assinatura espectral no infravermelho próximo de amostras dos grãos foi estabelecida regressão dos mínimos quadrados parciais (PLS-R) para estimar o teor de óleo nos grãos de canola. As épocas de semeadura influenciaram os componentes de produção e o teor de óleo dos grãos de todos híbridos. Os caracteres número de grãos em cinco plantas (0,6857) e altura (0,4943) apresentaram maiores estimativas de correlação positiva com a produtividade de grãos, assim como os maiores valores de efeito direto positivo sobre a produtividade, 0,2494 e 0,1595 respectivamente. Entretanto, o ciclo total (-0,7848), juntamente com dias em florescimento (-0,4520) apresentou correlação significativa negativa com a produtividade. A técnica NIR associada à PLS-R foi capaz de predizer o teor de óleo nos grãos, resultando em bons modelos preditivos (R2 de 0,86 e RMSE de 1,56 na validação externa) que podem ser usados com sucesso na análise da qualidade das amostras após colheita e nos programas de melhoramento genético.

Interação competitiva e nível de dano econômico de azevém daninho emhíbridos de canola / Competitive interaction and economic damage level of ryegrass harmfulg in canola hybrids

Na canola ocorrem perdas de produtividade e da qualidade de grãos em função da interferência causada pelo azevém (Lolium multiflorum). Diante disso, objetivou-se avaliar a interferência e identificar variáveis explicativas visando determinar o nível de dano econômico (NDE) de diferentes densidades do azevém infestante de híbridos de canola. O experimento foi instalado em delineamento de blocos casualizados, com uma repetição. Os tratamentos foram compostos pelos híbridos de canola (Hyola 433, Hyola 61, Alht B4, Hyola 575 CL e Hyola 76) e 12 densidades de azevém, de 0 até 260 plantas m-2, em competição com a cultura. Avaliou-se aos 50 dias após a emergência das plantas as variáveis densidades de plantas, área foliar, cobertura de solo e massa seca da parte aérea do azevém. A produtividade de grãos, custo de controle, preço de grãos e eficiência de controle foram determinados na canola. A cobertura do solo apresentou melhor ajuste ao modelo da hipérbole retangular estimando adequadamente as perdas de produtividades de grãos pela interferência do azevém. Os híbridos de canola Hyola 433, Hyola 575 CL e Hyola 76 foram os mais competitivos ao se comparar com os demais na presença do azevém, porém demonstraram as menores produtividades de grãos e também os que apresentaram os maiores valores de NDE. Os híbridos de canola Hyola 433 e Hyola 575 CL apresentaram os maiores valores de NDE com 3,85 a 5,13 plantas m-2 em todas as simulações realizadas, respectivamente. Os menores valores de NDE foram obtidos com os híbridos Hyola 61, Alht B4 e Hyola 76 com variações médias de 1,02 à 2,15 plantas m-2, respectivamente. A produtividade de grãos de canola, o preço da saca, a eficiência do herbicida e a redução no custo de controle, causam variação dos valores do NDE.(AU)

In canola, yield and grain quality losses occur due to the interference caused by ryegrass (Lolium multiflorum) when infesting the crop. Thus, the objective was to evaluate the interference and identify explanatory variables seeking to determine the level of economic damage (NDE) of different densities of ryegrass infesting canola hybrids. The experiment was carried out in a randomized block design, with one replication. The treatments consisted of five canola hybrids (Hyola 433, Hyola 61, Alht B4, Hyola 575 CL and Hyola 76) and 12 ryegrass densities, from 0 to 260 plants m-2, in competition with the crop. At 50 days after plant emergence, the variables as plant densities, leaf area, soil cover and shoot dry matter of ryegrass plants were evaluated. Grain yield, control cost, grain price and control efficiency were determined in canola plants. The soil cover presented the best fit to the rectangular hyperbola model, adequately estimating the grain yield losses due to ryegrass interference. The canola hybrids Hyola 433 and Hyola 575 CL were the most competitive when compared to the others in the presence of the competitor, with higher values of EDL. The canola hybrids Hyola 433 and Hyola 575 CL showed the highest EDL values with 3.85 to 5.13 plants m-2in all simulations performed, respectively. The lowest EDL values were obtained with hybrids Hyola 61, Alht B4and Hyola 76 with average variations of 1.02 to 2.15 plants m-2, respectively. The canola grain yield, grain bag price, efficiency of the herbicide, and the reduction in the control cost cause variation in the economic damage level values.(AU)

Manejo de mancha de alternária em canola em função de espaçamentos desemeadura e uso de fungicidas / Management of Alternaria spot in canola in function of sowing spaces and fungicides use

A mancha de alternária é uma das principais doenças da canola,reportada nas regiões produtoras da oleaginosa. Objetivou-se investigar se o espaçamento de semeadura em canola combinado com a aplicação de fungicidas produz efeito sobre os danos causados pela mancha de Alternária e como estes se refletem sobre o rendimento da cultura. O híbrido estudado foi o 'Nuola 300' e o delineamento experimental utilizado foi o de blocos ao acaso, em esquema de parcelas subdivididas, com quatro repetições. Nas parcelas principais foram dispostos os espaçamentos entre linhas (17 cm e 50 cm) e, nas subparcelas as aplicações de fungicidas (T1 -testemunha sem fungicida; T2 -azoxistrobina + tebuconazol, no estádio B4;T3 -azoxistrobina + tebuconazol, em B4 epiraclostrobina + fluxapiroxade, em B4 + 15 dias; T4 -piraclostrobina + fluxapiroxade, no estádio B4 + 15 dias). Nas condições de condução desse experimento,na região de Erechim ­RS, houve controle da mancha dealternária em folhas e síliquas de canola com a aplicação dos fungicidas azoxistrobina + tebuconazol e piraclostrobina + fluxapiroxade, sendo que o melhor controle em síliquas foi obtido no espaçamento de 17 cm entre linhas. O tratamento com piraclostrobina + fluxapiroxadeno espaçamento de 50 cm entre linhas apresentou maior número de síliquas por plantas, bem como maior produtividade.(AU)

The Alternaria spot is one of the main canola's diseases and it's reported in all regions that producing this oleaginous, being transmitted by several ways. The aimof this study was to investigate whether sowing spacing in canola combined with the fungicides applications hasan effect on the damage caused by Alternaria leaf spot and how these are reflected on crop yield. The studied hybrid was 'Nuola 300', and the experimental design used was randomized blocks with a subdivided plot scheme, with four replications. In the mainplots, the row spacing (17 cm and 50 cm) was arranged, and in the subplots, the fungicides applications (T1 -control, without fungicide; T2 -azoxystrobin + tebuconazole, in stage B4; T3 -azoxystrobin + tebuconazole, in B4 and pyraclostrobin + fluxapyroxad, in B4 + 15 days; T4 -pyraclostrobin + fluxapyroxade, in stage B4 + 15 days). Under conditions in which the experiment was conducted, in the region of Erechim -Rio Grande do Sul, Brazil, there were control of Alternaria spot in canola leaves and siliques with the application ofthe fungicides azoxystrobin + tebuconazole and pyraclostrobin + fluxapyroxade, and the best control in siliques was obtained at 17 cm row spacing. The treatment with pyraclostrobin + fluxapyroxade in the 50 cm row spacing showed the highest number of siliques by plantsand the highest productivity.(AU)

The Effect of Moringa Oleifera Leaf and Canola Seed Powder on Fattening, Laying Performance, Blood Plasma Constituents, and Microbiota in Japanese Quails

The aim of the study was to investigate the effect of feeding Japanese quail chicks with diets containing different levels of Moringa oleifera leaf and canola seed powder on growth performance, carcass yield, blood plasma constituents, and egg production. The trial lasted for a total of 7 weeks, with 5 weeks of fattening and two weeks of laying. The first group was the control group, while the second group was supplemented with Moringa oleifera leaves (4g/kg diet), the third group was supplemented with canola seed powder (4g/kg diet), the fourth group was supplemented with a mix of Moringa oleifera leaves and canola seed powder (8g/kg diet). The results showed that canola seed powder from 0-3 weeks of age increased body weight in comparison to the control group, but there were no significant differences (p<0.05) among groups in terms of the final body weight and feed conversion ratio. Average daily feed intake was significantly different (p<0.05). However, body weight, carcass weight, liver weight, gizzard weight, and abdominal fat weight increased significantly compared to the control group. Feeding Moringa oleifera leaves and canola seed powder significantly increased the total plasma protein, as compared to the control group. There were significant decreases in cholesterol, triglycerides and HDL levels among groups, with no significant differences in glucose, ALT and LDL among all treatments. The addition of Moringa oleifera leaf and canola seed powder showed significant effects on calcium and magnesium. Both Moringa oleifera leaf and Moringa-canola mixture significantly reduced the presence of some pathogenic bacteria in the digestive system, which was seen as an important contribution to the digestive and immune systems.(AU)

Comparative effectiveness of EDTA and citric acid assisted phytoremediation of Ni contaminated soil by using canola (Brassica napus).

Phytoremediation is an ecofriendly technique to clean heavy metals from contaminated soil by the use of high biomass producing plant species. Chelators can help to improve this biological technique by increasing metal solubility. Therefore, a pot experiment was conducted to determine the effect of the chelators EDTA and citric acid (CA) in phytoremediation of Ni contaminated soil by using Brassica napus (canola). Two cultivars of B. napus, Con-II (tolerant) and Oscar (sensitive), were selected after screening and exposed to NiSO4 at 30 ppm at the time of sowing. CA (10 mM) and EDTA (1.5 mM) were applied either alone or in combination with each other after two weeks of Ni treatments. Different parameters like morpho-physiological and biochemical data were recorded after 15 days of chelate application. The results highlighted the successful use of chelating agents (CA and EDTA) not only to ameliorate Ni stress but also to enhance Ni accumulation which is prerequisite for phytoremediation. The basal application of 10 mMCA and 1.5 mM EDTA concentration proved to be effective for the growth of plants. The combination of chelating agents failed to show any synergistic effects.

Risk Assessment of China Rapeseed Supply Chain and Policy Suggestions.

Rapeseed, as the most important oil crop in the world, not only affects national food security but also affects energy security and environmental security. It is very important to conduct a risk assessment of China's rapeseed supply chain and put forward suggestions to construct a safe, effective, and accessible supply chain. In order to accurately evaluate the safety of the rapeseed supply chain from 2010 to 2020, we applied fuzzy multiconnection theory and analytic hierarchy process model (AHP). A comprehensive risk assessment model for the rapeseed supply chain with two primary indicators and 10 secondary indicators was constructed. By establishing the rapeseed risk evaluation model, we quantitatively analyzed the risk of China's rapeseed supply chain. The domestic risk of production is still high, and the international risk under the high import dependence is alarming. We put forward risk prevention and countermeasures for China's rapeseed supply chain. The results show that China has a large demand for rapeseed products, but the increase in China production is limited and the import from other countries is unstable. The proposed suggestions are designed to optimize and enhance the stability of the rapeseed product's supply chain. It is recommended to continue to consolidate and deepen the cooperation with traditional trading partners such as Germany, Spain, the United States, and Brazil; expand other import sources to build a more diversified and efficient rapeseed product import network and extend the supply chain of rapeseed products. This research can be a basis for making decisions for promoting the sustainable and efficient development of the rapeseed supply chain.

Role of beneficial microbes with nitrogen and phosphorous levels on canola productivity / Papel de microbes benéficos em associação com níveis de nitrogênio e fósforo na produtividade de canola

A research was conducted to evaluate the impact of various nitrogen and phosphorus levels along with beneficial microbes to enhance canola productivity. The research was carried out at Agronomy Research Farm, The University of Agriculture Peshawar in winter 2016-2017. The experiment was conducted in randomized complete block factorial design. The study was comprised of three factors including nitrogen (60, 120 and 180 kg ha-¹), phosphorous (70, 100 and 130 kg ha-¹) and beneficial microbes (with and without BM). A control treatment with no N, P and BM was also kept for comparison. Application of beneficial microbes significantly increased pods plant, seed pod, seed filling duration, 1000 seed weight, biological yield and seed yield as compared to control plots. Nitrogen applied at the rate of 180 kg ha-¹ increased pods plant-¹, seed pod, seed filling duration, seed weight, biological yield and seed yield. Maximum pods plant-¹, seed pod, early seed filling, heavier seed weight, biological yield, seed yield, and harvest index were observed in plots treated with 130 kg.ha-¹ phosphorous. As comparison, the combine treated plots have more pods plant-¹, seeds pod-¹, seed filling duration, heaviest seeds, biological yield, seed yield and harvest index as compared to control plots. It is concluded that application of beneficial microbes with N and P at the rate of 180 kg ha-¹ and 130 kg ha-¹, respectively, increased yield and its attributes for canola.(AU)

Uma pesquisa foi realizada para avaliar o impacto de vários níveis de nitrogênio e fósforo, juntamente com micróbios benéficos, para aumentar a produtividade da canola. A pesquisa foi realizada no inverno de 2016-17 no Agronomy Research Farm, Universidade de Agricultura do Peshawar. O experimento foi conduzido por planejamento fatorial aleatorizado em blocos. O estudo focou-se em três fatores, incluindo o teor de nitrogênio, N, (60, 120 e 180 kg.ha-¹), o teor de fósforo, P, (70, 100 e 130 kg ha-¹) e a presença de micróbios benéficos (com BM e sem BM). Para fins de comparação, um tratamento controle sem N, P e BM também foi incluído no estudo. A aplicação de micróbios benéficos aumentou significativamente as vagens das plantas e de sementes, a duração do enchimento das sementes, o peso de 1000 sementes, o rendimento biológico e o rendimento de sementes em comparação com os resultados do controle. O nitrogênio aplicado na taxa de 180 kg ha-¹ aumentou as vagens por planta, vagem, duração do enchimento, peso da semente, rendimento biológico e rendimento de sementes. Vagens máximas por planta, vagem, enchimento precoce de sementes, peso maior de semente, rendimento biológico, rendimento de sementes e índice de colheita foram observados em parcelas tratadas com 130 kg.ha-¹ de fósforo. Em comparação aos blocos cultivados de controle, os blocos cultivados tratados combinados têm mais vagens por planta e sementes por vagem, maior duração do enchimento das sementes, maior número de sementes mais pesadas e maior rendimento biológico, rendimento de sementes e índice de colheita. Conclui-se que a aplicação de micróbios benéficos junto com N e P nas doses de 180 kg ha-¹ e 130 kg ha-¹, respectivamente, aumentou a produtividade e atributos de produtividade para a canola.(AU)

Comparative effectiveness of EDTA and citric acid assisted phytoremediation of Ni contaminated soil by using canola (Brassica napus) / Eficácia comparativa da fitorremediação assistida por EDTA e ácido cítrico de solo contaminado com Ni, usando canola (Brassica napus)

Phytoremediation is an ecofriendly technique to clean heavy metals from contaminated soil by the use of high biomass producing plant species. Chelators can help to improve this biological technique by increasing metal solubility. Therefore, a pot experiment was conducted to determine the effect of the chelators EDTA and citric acid (CA) in phytoremediation of Ni contaminated soil by using Brassica napus (canola). Two cultivars of B. napus, Con-II (tolerant) and Oscar (sensitive), were selected after screening and exposed to NiSO4 at 30 ppm at the time of sowing. CA (10 mM) and EDTA (1.5 mM) were applied either alone or in combination with each other after two weeks of Ni treatments. Different parameters like morpho-physiological and biochemical data were recorded after 15 days of chelate application. The results highlighted the successful use of chelating agents (CA and EDTA) not only to ameliorate Ni stress but also to enhance Ni accumulation which is prerequisite for phytoremediation. The basal application of 10 mMCA and 1.5 mM EDTA concentration proved to be effective for the growth of plants. The combination of chelating agents failed to show any synergistic effects.(AU)

A fitorremediação é uma técnica ecologicamente correta para limpar metais pesados ​​de solo contaminado pelo uso de espécies vegetais produtoras de alta biomassa. Os quelantes podem ajudar a melhorar esta técnica biológica aumentando a solubilidade do metal. Para tanto, foi realizado um experimento em vaso para determinar o efeito dos quelantes EDTA e ácido cítrico (AC) na fitorremediação de solo contaminado com Ni, utilizando Brassica napus (canola). Duas cultivares de B. napus, Con-II (tolerante) e Oscar (sensível) foram selecionadas após triagem e expostas a NiSO4 a 30 ppm no momento da semeadura. CA (10 mM) e EDTA (1,5 mM) foram aplicados sozinhos ou em combinação um com o outro após duas semanas de tratamentos com Ni. Diferentes parâmetros como dados morfofisiológicos e bioquímicos foram registrados após 15 dias de aplicação de quelato. Os resultados destacaram o uso bem-sucedido de agentes quelantes (CA e EDTA) não apenas para melhorar o estresse de Ni, mas também para aumentar o acúmulo de Ni, um pré-requisito para a fitorremediação. A aplicação basal da concentração de 10 mMCA e 1,5 mM de EDTA mostrou-se eficaz para o crescimento das plantas. A combinação de agentes quelantes não mostrou quaisquer efeitos sinérgicos.(AU)

Performance, gastrointestinal morphometry, carcass and non-carcass traits in sheep finished on diets containing canola (Brassica napus L)

The objective of this study was to evaluate performance, gastrointestinal morphometry, carcass, and non-carcass traits in lambs finished on different levels of canola grain in the diet. Twenty-seven Santa Ines lambs with an average initial weight of 19.33 ± 1.39 kg were given different levels of canola grain in the diet: 0, 8, and 16%. Weights and body condition scores were determined at the beginning, every 14 days and at the end of the experimental period. Animals were slaughtered to assess gastrointestinal morphometry, carcass and non-carcass traits. The statistical design used was completely randomized. The inclusion of canola grain showed a linear effect (0.723) for the width and height of ruminal papillae in the ventral region of the rumen. Intestinal villi and crypts showed a quadratic effect, with peaks of 0.62 µm and 0.43 µm, with the inclusion of 8 and 16%, respectively. When evaluating carcass traits, a decreasing linear effect was found for hot and cold carcass yield with 16% inclusion of canola. Canola grain can be used as an alternative in diets for finishing sheep up to 8% inclusion in the diet without affecting performance, ruminal and intestinal histometry, carcass, and non-carcass traits.(AU)