RESUMO
We determined the complete mitochondrial genome (GenBank accession number: KF709435) of the Korean raccoon dog Nyctereutes procyonoides koreensis and compared it with a previously published mitogenome (GenBank accession number: GU256221) of a Chinese raccoon dog. The total length of N. p. koreensis mitogenome is 16,802 bp, with a base composition of 32.1% A, 26.9% T, 26.8% C and 14.2% G. High similarity of 98.7% was found between the complete mitogenome sequences of Korean and Chinese raccoon dogs. Sequence similarity of the two mitogenomes was 99.3% in the other gene regions except for D-loop. The sequence similarity of 99.1% was found in the 13 protein-coding gene regions, whereas 99.6% was identical in mtDNA regions covering all the 22 tRNA genes. There was no variation between 12S rRNAs, whereas 0.5% difference was found between 16S rRNAs.
Assuntos
Genoma Mitocondrial , Cães Guaxinins/genética , Animais , Sequência de Bases , DNA Mitocondrial/genética , Especiação Genética , Dados de Sequência Molecular , Fases de Leitura Aberta/genética , RNA de Transferência/genética , Cães Guaxinins/classificação , Alinhamento de Sequência , Análise de Sequência de DNARESUMO
We have analysed the chromosomes of three wild and endangered canid species: the maned wolf (Chrysocyon brachyurus), the bat-eared fox (Otocyon megalotis) and the fennec fox (Fennecuszerda) using classical and molecular cytogenetic methods. For the first time detailed and encompassing descriptions of the chromosomes are presented including the chromosomal assignment of nucleolar organizer regions and the 5S rRNA gene cluster. We propose a karyotype nomenclature with ideograms including more than 300 bands per haploid set for each of these three species which will form the basis for further research. In addition, we propose four basic different patterns of karyotype organization in the family Canidae. A comparison of these patterns with the most recent molecular phylogeny of Canidae revealed that the karyotype evolution of a species is not always strongly connected with its phylogenetic position. Our findings underline the need and justification for basic cytogenetic work in rare and exotic species.
