RESUMO
Metalloid tellurium is characterized as a chemical element belonging to the chalcogen group without known biological function. However, its compounds, especially the oxyanions, exert numerous negative effects on both prokaryotic and eukaryotic organisms. Recent evidence suggests that increasing environmental pollution with tellurium has a causal link to autoimmune, neurodegenerative and oncological diseases. In this review, we provide an overview about the current knowledge on the mechanisms of tellurium compounds' toxicity in bacteria and humans and we summarise the various ways organisms cope and detoxify these compounds. Over the last decades, several gene clusters conferring resistance to tellurium compounds have been identified in a variety of bacterial species and strains. These genetic determinants exhibit great genetic and functional diversity. Besides the existence of specific resistance mechanisms, tellurium and its toxic compounds interact with molecular systems, mediating general detoxification and mitigation of oxidative stress. We also discuss the similarity of tellurium and selenium biochemistry and the impact of their compounds on humans.
Assuntos
Células Eucarióticas/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Células Procarióticas/efeitos dos fármacos , Telúrio/efeitos adversos , Ânions/efeitos adversos , Bactérias/efeitos dos fármacos , Poluição Ambiental/análise , Humanos , Selênio/química , Telúrio/química , Telúrio/toxicidadeRESUMO
Copper nanoparticles demonstrate antibacterial activity, but their toxicity to eukaryotic systems is less understood. Here, we carried out a comparative study to determine the biocompatibility and cytotoxicity of sub-10 nm copper nanoparticles to a variety of biological systems, including prokaryotic cells (Escherichia coli), yeast, mammalian cell lines (HEK293T, PC12), and zebrafish embryos. We determined the bearing threshold for the cell-death-inducing concentration of copper nanoparticles by probing cell growth, viability, as well as embryological features. To exclude the partial toxicity effect from the remnant reactants, we developed a purification approach using agarose gel electrophoresis. Purified CuONP solution inhibits bacterial growth and causes eukaryotic cell death at 170 and 122.5 ppm (w/w) during the 18 h of treatment, respectively. CuONP significantly reduces the pigmentation of retina pigmented epithelium of zebrafish embryos at 85 ppm. The cytotoxicity of CuONP in eukaryotic cells could arise from the oxidative stress induced by CuONP. This result suggests that small copper nanoparticles exert cytotoxicity in both prokaryotic and eukaryotic systems, and therefore, caution should be used to avoid direct contact of copper nanoparticles to human tissues considering the potential use of copper nanoparticles in the clinical setting.
Assuntos
Antibacterianos/farmacologia , Cobre/farmacologia , Células Eucarióticas/efeitos dos fármacos , Nanopartículas Metálicas/química , Epitélio Pigmentado Ocular/efeitos dos fármacos , Células Procarióticas/efeitos dos fármacos , Animais , Antibacterianos/química , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Cobre/química , Relação Dose-Resposta a Droga , Escherichia coli/citologia , Escherichia coli/efeitos dos fármacos , Células HEK293 , Humanos , Estrutura Molecular , Células PC12 , Tamanho da Partícula , Ratos , Saccharomyces cerevisiae/citologia , Saccharomyces cerevisiae/efeitos dos fármacos , Relação Estrutura-Atividade , Propriedades de Superfície , Peixe-Zebra/embriologiaRESUMO
Emerging widespread bacterial resistance to current antibiotics with traditional targets is one of the major global concerns. Therefore, so many investigations are exploring the potential of other druggable macromolecules of bacteria such as replication machinery components that are not addressed by previous antibiotics. DNA polymerase is the major part of this machine. However, a few studies have been done on it so far. In this respect, we report the discovery of four new plant-based leads against DNA polymerase (pol) IIIC (three leads) and pol IIIE (one lead) of Gram-positive and negative bacteria by combining a sequentially constrained high-throughput virtual screenings on Traditional Chinese Medicine Database with in vitro assays. The compounds displayed relatively good levels of inhibitory effect. They were active against their designated targets at micromolar concentrations. The IC50 values for them are ranged from 25 to 111 µM. In addition, they showed minimum inhibitory concentrations in the range of 8-128 µg/mL against five representatives of pathogenic bacteria species. However, they were inactive against Pseudomonas aeruginosa. Given these results, these leads hold promise for future modification and optimization to be more effective in lower concentrations and also against most of the important bacterial species. Communicated by Ramaswamy H. Sarma.
Assuntos
DNA Polimerase III/química , Replicação do DNA/efeitos dos fármacos , Chumbo/farmacologia , Inibidores da Síntese de Ácido Nucleico/farmacologia , Antibacterianos/efeitos adversos , Simulação por Computador , DNA Polimerase III/antagonistas & inibidores , Farmacorresistência Bacteriana/genética , Escherichia coli/efeitos dos fármacos , Escherichia coli/patogenicidade , Humanos , Chumbo/química , Testes de Sensibilidade Microbiana , Inibidores da Síntese de Ácido Nucleico/química , Células Procarióticas/efeitos dos fármacos , Células Procarióticas/microbiologia , Pseudomonas aeruginosa/efeitos dos fármacos , Pseudomonas aeruginosa/patogenicidadeRESUMO
Cyclic peptide scaffolds are key components of signal transduction pathways in both prokaryotic and eukaryotic organisms since they act as chemical messengers that activate or inhibit specific cognate receptors. In prokaryotic organisms these peptides are utilized in non-essential pathways, such as quorum sensing, that are responsible for virulence and pathogenicity. In the more evolved eukaryotic systems, cyclic peptide hormones play a key role in the regulation of the overall function of multicellular organisms, mainly through the endocrine system. This review will highlight several prokaryote and eukaryote systems that use cyclic peptides as their primary signals and the potential associated with utilizing these scaffolds for the discovery of novel therapeutics for a wide range of diseases and illnesses.
Assuntos
Peptídeos Cíclicos/farmacologia , Transdução de Sinais/efeitos dos fármacos , Sequência de Aminoácidos , Bactérias/efeitos dos fármacos , Bactérias/metabolismo , Células Eucarióticas/efeitos dos fármacos , Peptídeos Cíclicos/química , Células Procarióticas/efeitos dos fármacos , Percepção de Quorum/efeitos dos fármacosRESUMO
Graphene oxide (GO)-based materials are increasingly being used in medical materials and consumer products. However, their sublethal effects on biological systems are poorly understood. Here, we report that GO (at 10 to 160 mg/L) induced significant inhibitory effects on the growth of different unicellular organisms, including eukaryotes (i.e. Saccharomyces cerevisiae, Candida albicans, and Komagataella pastoris) and prokaryotes (Pseudomonas fluorescens). Growth inhibition could not be explained by commonly reported cytotoxicity mechanisms such as plasma membrane damage or oxidative stress. Based on transcriptomic analysis and measurement of extra- and intracellular iron concentrations, we show that the inhibitory effect of GO was mainly attributable to iron deficiency caused by binding to the O-functional groups of GO, which sequestered iron and disrupted iron-related physiological and metabolic processes. This inhibitory mechanism was corroborated with supplementary experiments, where adding bathophenanthroline disulfonate-an iron chelating agent-to the culture medium exerted similar inhibition, whereas removing surface O-functional groups of GO decreased iron sequestration and significantly alleviated the inhibitory effect. These findings highlight a potential indirect detrimental effect of nanomaterials (i.e. scavenging of critical nutrients), and encourage research on potential biomedical applications of GO-based materials to sequester iron and enhance treatment of iron-dependent diseases such as cancer and some pathogenic infections.
Assuntos
Proliferação de Células/efeitos dos fármacos , Grafite/toxicidade , Ferro/metabolismo , Nanoestruturas/toxicidade , Ciclo Celular/efeitos dos fármacos , Membrana Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Células Eucarióticas/efeitos dos fármacos , Células Eucarióticas/metabolismo , Grafite/química , Humanos , Nanoestruturas/química , Estresse Oxidativo/efeitos dos fármacos , Óxidos , Células Procarióticas/efeitos dos fármacos , Células Procarióticas/metabolismoRESUMO
Acute ecological impacts of co-contamination of polycyclic aromatic hydrocarbons (PAHs) and heavy metals on diversity and composition of coastal benthic prokaryotes were unclear. We took pyrene (Pyr) and cadmium (Cd) as the representatives and mimicked an eight-week exposure of moderate and high levels of Pyr, Cd and their mixtures. 16S rRNA amplicon sequencing was used to investigate interaction of the contaminants in temporal succession of prokaryotes. Generally, concentrations of Pyr and HCl-extractable Cd in the sediments were stable over time. Effects and interaction of Pyr and Cd on prokaryotic α-diversity were temporally- and dose-dependent with a decreasing trend in richness and Shannon index under various contamination regimes, particularly in the single-Cd contaminated groups at the early stage. Temporal variability and Pyr-induced pattern in prokaryotic composition were observed. However, Pyr and Cd showed a persistent interaction in prokaryotic composition after 7 days, altering successional trajectories of communities. The communities under Pyr contamination regardless of Cd could be at a developing stage for an active PAH-degrading community with appearance of a pioneer Cycloclasticus phylotype, persistently showing a strong correlation with Pyr level. The associations of phylotypes and Cd level were short-lived and weak, corresponding to the overall resistance of prokaryotic composition to Cd. In the high-throughput sequencing era, using microcosm experiment, we renewed the knowledge about how prokaryotes vary in terms of α-diversity, composition and specific taxa in response to co-contamination of model contaminants at a temporal scale.
Assuntos
Cádmio/farmacologia , Sedimentos Geológicos/microbiologia , Células Procarióticas/efeitos dos fármacos , Pirenos/farmacologia , Metais Pesados/química , Hidrocarbonetos Policíclicos Aromáticos/química , Células Procarióticas/química , RNA Ribossômico 16S/genéticaRESUMO
Streptolysin S (SLS)-like virulence factors from clinically relevant Gram-positive pathogens have been proposed to behave as potent cytotoxins, playing key roles in tissue infection. Listeriolysin S (LLS) is an SLS-like hemolysin/bacteriocin present among Listeria monocytogenes strains responsible for human listeriosis outbreaks. As LLS cytotoxic activity has been associated with virulence, we investigated the LLS-specific contribution to host tissue infection. Surprisingly, we first show that LLS causes only weak red blood cell (RBC) hemolysis in vitro and neither confers resistance to phagocytic killing nor favors survival of L. monocytogenes within the blood cells or in the extracellular space (in the plasma). We reveal that LLS does not elicit specific immune responses, is not cytotoxic for eukaryotic cells, and does not impact cell infection by L. monocytogenes Using in vitro cell infection systems and a murine intravenous infection model, we actually demonstrate that LLS expression is undetectable during infection of cells and murine inner organs. Importantly, upon intravenous animal inoculation, L. monocytogenes is found in the gastrointestinal system, and only in this environment LLS expression is detected in vivo Finally, we confirm that LLS production is associated with destruction of target bacteria. Our results demonstrate therefore that LLS does not contribute to L. monocytogenes tissue injury and virulence in inner host organs as previously reported. Moreover, we describe that LlsB, a putative posttranslational modification enzyme encoded in the LLS operon, is necessary for murine inner organ colonization. Overall, we demonstrate that LLS is the first SLS-like virulence factor targeting exclusively prokaryotic cells during in vivo infections.IMPORTANCE The most severe human listeriosis outbreaks are caused by L. monocytogenes strains harboring listeriolysin S (LLS), previously described as a cytotoxin that plays a critical role in host inner tissue infection. Cytotoxic activities have been proposed as a general mode of action for streptolysin S (SLS)-like toxins, including clostridiolysin S and LLS. We now challenge this dogma by demonstrating that LLS does not contribute to virulence in vivo once the intestinal barrier has been crossed. Importantly, we show that intravenous L. monocytogenes inoculation leads to bacterial translocation to the gastrointestinal system, where LLS is specifically expressed, targeting the host gut microbiota. Our study highlights the heterogeneous modes of action of SLS-like toxins, and we demonstrate for the first time a further level of complexity for SLS-like biosynthetic clusters as we reveal that the putative posttranslational modification enzyme LlsB is actually required for inner organ colonization, independently of the LLS activity.
Assuntos
Proteínas Hemolisinas/metabolismo , Interações Hospedeiro-Patógeno , Listeria monocytogenes/patogenicidade , Listeriose/microbiologia , Listeriose/patologia , Células Procarióticas/efeitos dos fármacos , Fatores de Virulência/metabolismo , Animais , Células Cultivadas , Modelos Animais de Doenças , Humanos , Camundongos , Viabilidade Microbiana/efeitos dos fármacosRESUMO
There is much controversy about the metabolic state of cells that are tolerant to antibiotics, known as persister cells. In this opinion piece, we offer an explanation for the discrepancy seen: some laboratories are studying metabolically active and growing cell populations (e.g., as a result of nutrient shifts) and attributing the phenotypes that they discern to persister cells while other labs are studying dormant cells. We argue here that the metabolically active cell population should more accurately be considered tolerant cells, while the dormant cells are the true persister population.
Assuntos
Antibacterianos/farmacologia , Tolerância a Medicamentos , Células Procarióticas/efeitos dos fármacos , Células Procarióticas/fisiologia , Células Procarióticas/classificaçãoRESUMO
Tons of anthropogenic silver nanoparticles (AgNPs) are assumed to be released into the environment due to their use in many consumer products. AgNPs are known to be toxic toward microorganisms and thus may harm their specific functions in ecosystems. Here we explore the impact of AgNPs on functioning of single cells in microbial populations at doses typically found in anthropogenic environments. The response of single cells to AgNPs was analyzed by flow cytometry and using the fluorescent dyes propidium iodide and DiBAC4 (3) as markers for cell membrane disintegration and depolarization, respectively. The effects of 10-nm and 30-nm AgNPs on three bacterial species (Mycobacterium frederiksbergense, Pseudomonas putida, and Escherichia coli) showed that the populations split into affected cells and others not showing any malfunction, with varying abundances depending on strains and cell growth states. Further, the dissolution of AgNPs measured with 3 KDa ultrafiltration and inductively coupled plasma-mass-spectrometry to distinguish particle-related effects from toxicity of dissolved Ag revealed that Ag ions were the principal toxicant. AgNP aggregate formation was followed by dynamic light scattering and the aggregates' attachment to cell surfaces was visualized by transmission electron microscopy and scanning electron microscopy-energy dispersive X-ray spectroscopy. An increased AgNP-affected cell fraction relative to the Ag ion impact was identified. The study shows that individual cells in a population cope differently with AgNP induced stress by evolving heterogeneous phenotypes. The response is linked to cell death and cell energy depletion depending on cell type and cell growth states. The attachment of AgNP aggregates to cell surfaces seems to amplify the heterogeneous response. © 2017 International Society for Advancement of Cytometry.
Assuntos
Íons/administração & dosagem , Nanopartículas Metálicas/administração & dosagem , Células Procarióticas/efeitos dos fármacos , Prata/administração & dosagem , Bactérias/efeitos dos fármacos , Morte Celular/efeitos dos fármacos , Membrana Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Corantes Fluorescentes/administração & dosagem , Espectrometria de Massas/métodos , Microscopia Eletrônica de Transmissão/métodos , Tamanho da Partícula , Fenótipo , Espectrometria por Raios X/métodosRESUMO
The biocide Irgarol 1051 has been reported to have negative effects on a large number of living components including non-target organisms, but information on its impact on the marine meiofauna and benthic prokaryotes is completely lacking. Here, we report the results of long-term experimental studies in which we determined the effects of increasing Irgarol concentrations (from 11.5 to 315 ng g(-1) sediment dry weight) on meiofauna and benthic prokaryotes. We found that this biocide had a significant impact on meiofauna abundance, even at the lowest concentrations, causing a drastic decline in the abundance of nematodes (the dominant meiofaunal taxon) and an increase of the relative importance of oligochaetes. Even if no direct effects of Irgarol were found on prokaryotic abundance and biomass, the molecular fingerprinting analyses (automated ribosomal intergenic spacer analysis) showed that the prokaryotic diversity was significantly altered by the biocide. The results of the present study indicate that Irgarol 1051 in marine sediments has a significant impact on the smallest eukaryotic and microbial components also at very low concentrations (ca 12 ng g(-1)).
Assuntos
Desinfetantes/toxicidade , Sedimentos Geológicos , Nematoides/efeitos dos fármacos , Células Procarióticas/efeitos dos fármacos , Triazinas/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , TunísiaRESUMO
Little knowledge is available about the potential impact of commercial silver nanoparticles (Ag-NPs) on estuarine microbial communities. The Hugli river estuary, India, is susceptible to heavy metals pollution through boat traffic, and there is the potential for Ag-NP exposure via effluent discharged from ongoing municipal and industrial activities located in close proximity. This study investigated the effects of commercial Ag-NPs on native microbial communities in estuarine sediments collected from five stations, using terminal restriction fragment length polymorphism (T-RFLP) technique. An increase in the number of bacteria in consortium in sediments was observed following exposure to Ag-NPs. In general microbial communities may be resistant in estuarine systems to the antimicrobial effects of commercial Ag-NPs, but key microorganisms, such as Pelobacter propionicus, disappeared following exposure to Ag-NPs. In conclusion, the T-RFLP analysis indicated that Ag-NPs have the potential to shape estuarine sediment bacterial community structure.
Assuntos
Sedimentos Geológicos/microbiologia , Consórcios Microbianos/efeitos dos fármacos , Nanopartículas/toxicidade , Prata/toxicidade , Poluentes Químicos da Água/toxicidade , Bactérias/efeitos dos fármacos , Meio Ambiente , Estuários , Índia , Metais Pesados/toxicidade , Methanospirillum/efeitos dos fármacos , Consórcios Microbianos/genética , Polimorfismo de Fragmento de Restrição , Células Procarióticas/efeitos dos fármacos , Rios , Prata/química , Clima Tropical , Poluentes Químicos da Água/análiseRESUMO
Prokaryote respiration is expected to be responsible for more than half of the community respiration in the ocean, but the lack of a practical method to measure the rate of prokaryote respiration in the open ocean resulted in very few published data leaving the role of organotrophic prokaryotes open to debate. Oxygen consumption rates of oceanic prokaryotes measured with current methods may be biased due to pre-incubation size filtration and long incubation times both of which can change the physiological and taxonomic profile of the sample during the incubation period. In vivo INT reduction has been used in terrestrial samples to estimate respiration rates, and recently, the method was introduced and applied in aquatic ecology. We measured oxygen consumption rates and in vivo INT reduction to formazan in cultures of marine bacterioplankton communities, Vibrio harveyi and the eukaryote Isochrysis galbana. For prokaryotes, we observed a decrease in oxygen consumption rates with increasing INT concentrations between 0.05 and 1 mM. Time series after 0.5 mM INT addition to prokaryote samples showed a burst of in vivo INT reduction to formazan and a rapid decline of oxygen consumption rates to zero within less than an hour. Our data for non-axenic eukaryote cultures suggest poisoning of the eukaryote. Prokaryotes are clearly poisoned by INT on time scales of less than 1 h, invalidating the interpretation of in vivo INT reduction to formazan as a proxy for oxygen consumption rates.
Assuntos
Respiração Celular/efeitos dos fármacos , Consumo de Oxigênio/efeitos dos fármacos , Células Procarióticas/efeitos dos fármacos , Sais de Tetrazólio/toxicidade , Microbiologia da Água , Bactérias Aeróbias/efeitos dos fármacos , Bactérias Aeróbias/metabolismo , Ecossistema , Eucariotos/efeitos dos fármacos , Formazans/análise , Formazans/metabolismo , Haptófitas/efeitos dos fármacos , Haptófitas/metabolismo , Biologia Marinha/métodos , Oceanos e Mares , Oxirredução , Plâncton/efeitos dos fármacos , Plâncton/metabolismo , Células Procarióticas/citologia , Células Procarióticas/metabolismo , Vibrio/efeitos dos fármacos , Vibrio/metabolismoRESUMO
In prokaryotic species equipped with glycogen metabolism machinery, the co-regulation of glycogen biosynthesis and degradation has been associated with the synthesis of energy storage compounds and various crucial physiological functions, including global cellular processes such as carbon and nitrogen metabolism, energy sensing and production, stress response and cell-cell communication. In addition, the glycogen metabolic pathway was proposed to serve as a carbon capacitor that regulates downstream carbon fluxes, and in some microorganisms the ability to synthesize intracellular glycogen has been implicated in host persistence. Among lactobacilli, complete glycogen metabolic pathway genes are present only in select species predominantly associated with mammalian hosts or natural environments. This observation highlights the potential involvement of glycogen biosynthesis in probiotic activities and persistence of intestinal lactobacilli in the human gastrointestinal tract. In this review, we summarize recent findings on (i) the presence and potential ecological distribution of glycogen metabolic pathways among lactobacilli, (ii) influence of carbon substrates and growth phases on glycogen metabolic gene expression and glycogen accumulation in L. acidophilus, and (iii) the involvement of glycogen metabolism on growth, sugar utilization and bile tolerance. Our present in vivo studies established the significance of glycogen biosynthesis on the competitive retention of L. acidophilus in the mouse intestinal tract, demonstrating for the first time that the ability to synthesize intracellular glycogen contributes to gut fitness and retention among probiotic microorganisms.
Assuntos
Adaptação Fisiológica , Metabolismo dos Carboidratos , Trato Gastrointestinal/microbiologia , Glicogênio/metabolismo , Lactobacillus acidophilus/metabolismo , Estresse Fisiológico , Adaptação Fisiológica/efeitos dos fármacos , Animais , Metabolismo dos Carboidratos/efeitos dos fármacos , Carbono/farmacologia , Contagem de Colônia Microbiana , Trato Gastrointestinal/efeitos dos fármacos , Glicogênio/biossíntese , Humanos , Lactobacillus acidophilus/efeitos dos fármacos , Lactobacillus acidophilus/genética , Lactobacillus acidophilus/crescimento & desenvolvimento , Redes e Vias Metabólicas/efeitos dos fármacos , Redes e Vias Metabólicas/genética , Camundongos , Modelos Biológicos , Mutação/genética , Óperon/genética , Células Procarióticas/efeitos dos fármacos , Células Procarióticas/metabolismo , Rafinose/farmacologia , Especificidade da Espécie , Estresse Fisiológico/efeitos dos fármacos , Trealose/farmacologiaRESUMO
Human growth hormone (hGH) is synthesized by somatotroph cells of the anterior pituitary gland and induces cell proliferation and growth. This protein has been approved for the treatment of various conditions, including hGH deficiency, chronic renal failure, and Turner syndrome. Efficient production of hGH in Escherichia coli (E. coli) has proven difficult because the E. coli-expressed hormone tends to aggregate and form inclusion bodies, resulting in poor solubility. In this study, seven N-terminal fusion partners, hexahistidine (His6), thioredoxin (Trx), glutathione S-transferase (GST), maltose-binding protein (MBP), N-utilization substance protein A (NusA), protein disulfide bond isomerase (PDI), and the b'a' domain of PDI (PDIb'a'), were tested for soluble overexpression of codon-optimized hGH in E. coli. We found that MBP and hPDI tags significantly increased the solubility of the hormone. In addition, lowering the expression temperature to 18°C also dramatically increased the solubility of all the fusion proteins. We purified hGH from MBP-, PDIb'a'-, or Trx-tagged hGH expressed at 18°C in E. coli using simple chromatographic techniques and compared the final purity, yield, and activity of hGH to assess the impact of each partner protein. Purified hGH was highly pure on silver-stained gel and contained very low levels of endotoxin. On average, â¼37 mg, â¼12 mg, and â¼7 mg of hGH were obtained from 500 mL-cell cultures of Trx-hGH, MBP-hGH, and PDIb'a'-hGH, respectively. Subsequently, hGH was analyzed using mass spectroscopy to confirm the presence of two intra-molecular disulfide bonds. The bioactivity of purified hGHs was demonstrated using Nb2-11 cell.
Assuntos
Hormônio do Crescimento Humano/isolamento & purificação , Proteínas Ligantes de Maltose/metabolismo , Células Procarióticas/metabolismo , Isomerases de Dissulfetos de Proteínas/metabolismo , Proteínas Recombinantes de Fusão/isolamento & purificação , Tiorredoxinas/metabolismo , Sequência de Aminoácidos , Animais , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Escherichia coli/metabolismo , Hormônio do Crescimento Humano/química , Hormônio do Crescimento Humano/farmacologia , Humanos , Dados de Sequência Molecular , Plasmídeos/metabolismo , Células Procarióticas/efeitos dos fármacos , Estabilidade Proteica/efeitos dos fármacos , Estrutura Terciária de Proteína , Ratos , Proteínas Recombinantes de Fusão/química , Proteínas Recombinantes de Fusão/farmacologia , Solubilidade , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Frações Subcelulares/efeitos dos fármacos , Frações Subcelulares/metabolismoRESUMO
Sediments from the Athabasca River and its tributaries naturally contain bitumen at various concentrations, but the impacts of this variation on the ecology of the river are unknown. Here, we used controlled rotating biofilm reactors in which we recirculated diluted sediments containing various concentrations of bituminous compounds taken from the Athabasca River and three tributaries. Biofilms exposed to sediments having low and high concentrations of bituminous compounds were compared. The latter were 29% thinner, had a different extracellular polysaccharide composition, 67% less bacterial biomass per µm2, 68% less cyanobacterial biomass per µm2, 64% less algal biomass per µm2, 13% fewer protozoa per cm2, were 21% less productive, and had a 33% reduced content in chlorophyll a per mm2 and a 20% reduction in the expression of photosynthetic genes, but they had a 23% increase in the expression of aromatic hydrocarbon degradation genes. Within the Bacteria, differences in community composition were also observed, with relatively more Alphaproteobacteria and Betaproteobacteria and less Cyanobacteria, Bacteroidetes, and Firmicutes in biofilms exposed to high concentrations of bituminous compounds. Altogether, our results suggest that biofilms that develop in the presence of higher concentrations of bituminous compounds are less productive and have lower biomass, linked to a decrease in the activities and abundance of photosynthetic organisms likely due to inhibitory effects. However, within this general inhibition, some specific microbial taxa and functional genes are stimulated because they are less sensitive to the inhibitory effects of bituminous compounds or can degrade and utilize some bitumen-associated compounds.
Assuntos
Biofilmes/efeitos dos fármacos , Biota/efeitos dos fármacos , Células Eucarióticas/efeitos dos fármacos , Hidrocarbonetos/toxicidade , Células Procarióticas/efeitos dos fármacos , Rios/microbiologia , Rios/parasitologiaRESUMO
Microbial drug persistence is a widespread phenomenon in which a subpopulation of microorganisms is able to survive antimicrobial treatment without acquiring resistance-conferring genetic changes. Microbial persisters can cause recurrent or intractable infections, and, like resistant mutants, they carry an increasing clinical burden. In contrast to heritable drug resistance, however, the biology of persistence is only beginning to be unraveled. Persisters have traditionally been thought of as metabolically dormant, nondividing cells. As discussed in this review, increasing evidence suggests that persistence is in fact an actively maintained state, triggered and enabled by a network of intracellular stress responses that can accelerate processes of adaptive evolution. Beyond shedding light on the basis of persistence, these findings raise the possibility that persisters behave as an evolutionary reservoir from which resistant organisms can emerge. As persistence and its consequences come into clearer focus, so too does the need for clinically useful persister-eradication strategies.
Assuntos
Resistência Microbiana a Medicamentos , Células Eucarióticas/efeitos dos fármacos , Células Procarióticas/efeitos dos fármacos , Estresse FisiológicoRESUMO
Life on Earth is limited by physical and chemical extremes that define the 'habitable space' within which it operates. Aside from its requirement for liquid water, no definite limits have been established for life under any extreme. Here, we employ growth data published for 67 prokaryotic strains to explore the limitations for microbial life under combined extremes of temperature, pH, salt (NaCl) concentrations, and pressure. Our review reveals a fundamental lack of information on the tolerance of microorganisms to multiple extremes that impedes several areas of science, ranging from environmental and industrial microbiology to the search for extraterrestrial life.
Assuntos
Viabilidade Microbiana/efeitos dos fármacos , Viabilidade Microbiana/efeitos da radiação , Células Procarióticas/fisiologia , Concentração de Íons de Hidrogênio , Pressão Hidrostática , Células Procarióticas/efeitos dos fármacos , Células Procarióticas/efeitos da radiação , Salinidade , TemperaturaRESUMO
Disruption of cellular production of the flavin cofactors, flavin adenine mononucleotide (FMN) and flavin adenine dinucleotide(FAD) will prevent the assembly of a large number of flavoproteins and flavoenzymes involved in key metabolic processes in all types of organisms. The enzymes responsible for FMN and FAD production in prokaryotes and eukaryotes exhibit various structural characteristics to catalyze the same chemistry, a fact that converts the prokaryotic FAD synthetase (FADS) in a potential drug target for the development of inhibitors endowed with anti-pathogenic activity. The first step before searching for selective inhibitors of FADS is to understand the structural and functional mechanisms for the riboflavin kinase and FMN adenylyltransferase activities of the prokaryotic enzyme, and particularly to identify their differential functional characteristics with regard to the enzymes performing similar functions in other organisms, particularly humans. In this paper, an overview of the current knowledge of the structure-function relationships in prokaryotic FADS has been presented, as well as of the state of the art in the use of these enzymes as drug targets.
Assuntos
Descoberta de Drogas , Nucleotidiltransferases/antagonistas & inibidores , Nucleotidiltransferases/química , Células Procarióticas/enzimologia , Animais , Sítios de Ligação , Mononucleotídeo de Flavina/metabolismo , Flavina-Adenina Dinucleotídeo/metabolismo , Humanos , Modelos Moleculares , Células Procarióticas/efeitos dos fármacos , Células Procarióticas/metabolismo , Conformação Proteica , Dobramento de ProteínaRESUMO
Today, a material science focuses on the nanoparticles synthesis in general and synthesizing them by biological entity in particular for their marvel production and its remarkable property. In this present study, synthesis of gold nanoparticles using photosynthetic microorganisms such as Coelastrella sp. (eukaryotes) and Phormidium sp. (prokaryotes) were reacted with Chloroauric acid (HAuCl(4)) and bioaccumulation was assessed. Various techniques were adopted for characterization of nanoparticles and compared. It was found to be 25 nm sized nanotriangles and 30 nm sized spherical shaped nanoparticles were synthesized by prokaryotic and eukaryotic microorganisms respectively by TEM analysis. Biogenic gold nanoparticles have potent antioxidant property and the interaction of gold nanoparticles with DNA was evaluated that biogenic nanoparticles were actively bound to DNA in increased concentration. It was revealed that biogenic nanoparticles have wide range of applications depends on the biological entity used. Selection of suitable biological entity is very much important for the production of nanoparticles with desirable shapes and size for the biomedical applications.
Assuntos
Células Eucarióticas/química , Ouro/química , Nanopartículas Metálicas/química , Fotossíntese , Células Procarióticas/química , Coloração e Rotulagem , Antioxidantes/farmacologia , Cianobactérias/química , Cianobactérias/citologia , Cianobactérias/efeitos dos fármacos , Cianobactérias/ultraestrutura , DNA/metabolismo , Eletroforese em Gel de Ágar , Células Eucarióticas/citologia , Células Eucarióticas/efeitos dos fármacos , Células Eucarióticas/ultraestrutura , Nanopartículas Metálicas/ultraestrutura , Microalgas/química , Microalgas/citologia , Microalgas/efeitos dos fármacos , Microalgas/ultraestrutura , Tamanho da Partícula , Fotossíntese/efeitos dos fármacos , Células Procarióticas/citologia , Células Procarióticas/efeitos dos fármacos , Células Procarióticas/ultraestrutura , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
This work is focused on the fabrication of a new drug delivery system based on polyanionic matrix (e.g. sodium alginate), polycationic matrix (e.g. chitosan) and silica network. The FT-IR, SEM, DTA-TG, eukaryotic cell cycle and viability, and in vitro assay of the influence of the biocomposite on the efficacy of antibiotic drugs were investigated. The obtained results demonstrated the biocompatibility and the ability of the fabricated biocomposite to maintain or improve the efficacy of the following antibiotics: piperacillin-tazobactam, cefepime, piperacillin, imipenem, gentamicin, ceftazidime against Pseudomonas aeruginosa ATCC 27853 and cefazolin, cefaclor, cefuroxime, ceftriaxone, cefoxitin, trimethoprim/sulfamethoxazole against Escherichia coli ATCC 25922 reference strains.