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1.
Proc Natl Acad Sci U S A ; 115(34): 8633-8638, 2018 08 21.
Artigo em Inglês | MEDLINE | ID: mdl-30082394

RESUMO

The human microbiome is an assemblage of diverse bacteria that interact with one another to form communities. Bacteria in a given community are arranged in a 3D matrix with many degrees of freedom. Snapshots of the community display well-defined structures, but the steps required for their assembly are not understood. Here, we show that this construction is carried out with the help of gliding bacteria. Gliding is defined as the motion of cells over a solid or semisolid surface without the necessity of growth or the aid of pili or flagella. Genomic analysis suggests that gliding bacteria are present in human microbial communities. We focus on Capnocytophaga gingivalis, which is present in abundance in the human oral microbiome. Tracking of fluorescently labeled single cells and of gas bubbles carried by fluid flow shows that swarms of C. gingivalis are layered, with cells in the upper layers moving more rapidly than those in the lower layers. Thus, cells also glide on top of one another. Cells of nonmotile bacterial species attach to the surface of C. gingivalis and are propelled as cargo. The cargo cell moves along the length of a C. gingivalis cell, looping from one pole to the other. Multicolor fluorescent spectral imaging of cells of different live but nonmotile bacterial species reveals their long-range transport in a polymicrobial community. A swarm of C. gingivalis transports some nonmotile bacterial species more efficiently than others and helps to shape the spatial organization of a polymicrobial community.


Assuntos
Capnocytophaga/fisiologia , Consórcios Microbianos/fisiologia , Microbiota/fisiologia , Boca/microbiologia , Humanos
2.
Rev Med Interne ; 39(10): 820-823, 2018 Oct.
Artigo em Francês | MEDLINE | ID: mdl-29706238

RESUMO

INTRODUCTION: Capnocytophaga canimorsus is a Gram-negative bacterium present in normal dogs and cats mouths. It can be responsible for septic shocks after dog or cat bite, especially in asplenic patients. CASE REPORT: We report here a case of C. canimorsus infection rapidly presenting as a multiple organ dysfonction syndrome in an immunocompetent 54 years old patient, who lives near a dog, without any sign of bite. The evolution was a rapidly fatal fulminant septic shock. CONCLUSION: Among patients with a septic shock of unknown etiology, fatal evolution due to C. canimorsus should be avoided by systematic use of early antibiotherapy with amoxicilline and clavulanic acid, especially in patients who live near a pet, even if they are immunocompetent.


Assuntos
Capnocytophaga/fisiologia , Infecções por Bactérias Gram-Negativas/complicações , Choque Séptico/microbiologia , Animais , Capnocytophaga/isolamento & purificação , Cães , Evolução Fatal , Humanos , Masculino , Pessoa de Meia-Idade , Choque Séptico/diagnóstico , Zoonoses/diagnóstico , Zoonoses/microbiologia
3.
Acta Microbiol Immunol Hung ; 63(1): 103-13, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-27020873

RESUMO

To assess the prevalence and occurrence of eleven periodontopathogens in subgingival biofilm of banded and bonded molars during the first period of fixed orthodontic treatment. Subjects were selected from patients referred to orthodontic treatment and were divided in two groups: group A comprised fifteen patients (14.4±2.45 years of age) who received orthodontic bands on first permanent molars and group B of ten patients (15.7±1.87 years of age) with directly bonded tubes on the labial surface of the same teeth. Subgingival sample collection was performed before bands and tubes application and 4-7 weeks after attachment placement. DNA-strip tehnique was used to assess the presence of eleven putative periodontopathogens at each time point. Fusobacterium nucleatum, Eikenella corrodens and Capnocytophaga spp. were found in a large number of samples, other periodontopathogens were present in a smaller rate. The 4-7 weeks after attachment placement a slight increase of putative species was observed in both groups. The presence of orthodontic tubes and bands influence the accumulation and composition of subgingival microbiota. Higher level of oral hygiene should be achieved before and during orthodontic treatment in order to prevent any side effects on periodontal tissues.


Assuntos
Biofilmes , Capnocytophaga/fisiologia , Placa Dentária/microbiologia , Eikenella corrodens/fisiologia , Fusobacterium nucleatum/fisiologia , Dente Molar/microbiologia , Adolescente , Capnocytophaga/isolamento & purificação , Criança , Placa Dentária/terapia , Eikenella corrodens/isolamento & purificação , Feminino , Fusobacterium nucleatum/isolamento & purificação , Humanos , Masculino , Ortodontia
4.
Appl Environ Microbiol ; 82(6): 1756-1766, 2016 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-26729712

RESUMO

Capnocytophaga ochracea is a Gram-negative, rod-shaped bacterium that demonstrates gliding motility when cultured on solid agar surfaces. C. ochracea possesses the ability to form biofilms; however, factors involved in biofilm formation by this bacterium are unclear. A type IX secretion system (T9SS) in Flavobacterium johnsoniae was shown to be involved in the transport of proteins (e.g., several adhesins) to the cell surface. Genes orthologous to those encoding T9SS proteins in F. johnsoniae have been identified in the genome of C. ochracea; therefore, the T9SS may be involved in biofilm formation by C. ochracea. Here we constructed three ortholog-deficient C. ochracea mutants lacking sprB (which encodes a gliding motility adhesin) or gldK or sprT (which encode T9SS proteins in F. johnsoniae). Gliding motility was lost in each mutant, suggesting that, in C. ochracea, the proteins encoded by sprB, gldK, and sprT are necessary for gliding motility, and SprB is transported to the cell surface by the T9SS. For the ΔgldK, ΔsprT, and ΔsprB strains, the amounts of crystal violet-associated biofilm, relative to wild-type values, were 49%, 34%, and 65%, respectively, at 48 h. Confocal laser scanning and scanning electron microscopy revealed that the biofilms formed by wild-type C. ochracea were denser and bacterial cells were closer together than in those formed by the mutant strains. Together, these results indicate that proteins exported by the T9SS are key elements of the gliding motility and biofilm formation of C. ochracea.


Assuntos
Sistemas de Secreção Bacterianos/metabolismo , Biofilmes/crescimento & desenvolvimento , Capnocytophaga/fisiologia , Locomoção , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Sistemas de Secreção Bacterianos/genética , Capnocytophaga/genética , Técnicas de Inativação de Genes
5.
PLoS One ; 11(1): e0147114, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26800339

RESUMO

Capnocytophaga ochracea is present in the dental plaque biofilm of patients with periodontitis. Biofilm cells change their phenotype through quorum sensing in response to fluctuations in cell-population density. Quorum sensing is mediated by auto-inducers (AIs). AI-2 is involved in intercellular signaling, and production of its distant precursor is catalyzed by LuxS, an enzyme involved in the activated methyl cycle. Our aim was to clarify the role of LuxS in biofilm formation by C. ochracea. Two luxS-deficient mutants, TmAI2 and LKT7, were constructed from C. ochracea ATCC 27872 by homologous recombination. The mutants produced significantly less AI-2 than the wild type. The growth rates of these mutants were similar to that of the wild-type in both undiluted Tryptic soy broth and 0.5 × Tryptic soy broth. However, according to crystal violet staining, they produced significantly less biofilm than the wild type. Confocal laser scanning microscopy and scanning electron microscopy showed that the biofilm of the TmAI2 strain had a rougher structure than that of the wild type. Complementation of TmAI-2 with extrinsic AI-2 from the culture supernatant of wild-type strain did not restore biofilm formation by the TmAI2 strain, but complementation of LKT7 strain with luxS partially restored biofilm formation. These results indicate that LuxS is involved in biofilm formation by C. ochracea, and that the attenuation of biofilm formation by the mutants is likely caused by a defect in the activated methyl cycle rather than by a loss of AI-2.


Assuntos
Proteínas de Bactérias/fisiologia , Biofilmes , Capnocytophaga/fisiologia , Liases de Carbono-Enxofre/fisiologia , Proteínas de Bactérias/genética , Capnocytophaga/genética , Liases de Carbono-Enxofre/genética , Recombinação Homóloga , Microscopia Confocal , Microscopia Eletrônica de Varredura , Mutação
6.
Anaerobe ; 18(1): 157-61, 2012 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22252100

RESUMO

The formation of dental plaque biofilm by specific Gram-negative rods and spirochetes plays an important role in the development of periodontal disease. The aim of this study was to characterize biofilm formation by Fusobacterium nucleatum and Capnocytophaga ochracea. Coaggregation between F. nucleatum and Capnocytophaga species was determined by visual assay. Biofilm formation was assessed by crystal violet staining. Enhancement of biofilm formation by F. nucleatum via soluble factor of C. ochracea was evaluated by addition of culture supernatant and a two-compartment separated co-culture system. Production of autoinducer-2 by the tested organisms was evaluated using Vibrio harveyi BB170. F. nucleatum strains coaggregated with C. ochracea ATCC 33596 or ONO-26 strains. Ethylenediamine tetraacetic acid, N-acetyl-d-galactosamine or lysine inhibited coaggregation. Heating or proteinase K treatment of F. nucleatum cells affected coaggregation, whereas the same treatment of C. ochracea cells did not. Co-culture of F. nucleatum with C. ochracea in the same well resulted in a statistically significant increase in biofilm formation. Enhancement of F. nucleatum biofilm formation by a soluble component of C. ochracea was observed using the two-compartment co-culture system (P < 0.05) and confirmed by addition of culture supernatant of C. ochracea (P < 0.01). The present findings indicate that induction of coaggregation and intracellular interaction by release of a diffusible molecule by C. ochracea play a significant role in the formation of biofilm by F. nucleatum and C. ochracea.


Assuntos
Biofilmes/crescimento & desenvolvimento , Capnocytophaga/fisiologia , Fusobacterium nucleatum/fisiologia , Aminoácidos/farmacologia , Aderência Bacteriana/efeitos dos fármacos , Carboidratos/farmacologia , Quelantes/farmacologia , Técnicas de Cocultura , Ácido Edético/farmacologia , Endopeptidase K/química , Homosserina/análogos & derivados , Homosserina/biossíntese , Temperatura Alta , Lactonas , Simbiose/fisiologia
7.
Mol Oral Microbiol ; 26(1): 52-61, 2011 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-21214872

RESUMO

Recently, the predation of Bdellovibrio bacteriovorus on a periodontal pathogen has been described. The current study explores the potential antimicrobial activity of a range of predatory bacteria against key periodontal pathogens. A number of representatives from the Bdellovibrio, Bacteriovorax and Peredibacter lineages (called 'BALOs') were tested for their activity towards a group of key periodontal pathogens and an optimal multiplicity of infection was established. As the oral cavity contains a wide variety of bacteria that are not preyed upon, it was investigated if they can have an effect on the predation efficiency of BALOs. It was concluded that a number of important variables involved in bacterial predation are found to be compatible with the composition of the oral microbiota. This finding makes the case for continued study of the potential for BALOs to combat periodontal pathogens.


Assuntos
Antibiose/fisiologia , Bdellovibrio/fisiologia , Deltaproteobacteria/fisiologia , Boca/microbiologia , Doenças Periodontais/microbiologia , Actinomyces/fisiologia , Aggregatibacter actinomycetemcomitans/fisiologia , Capnocytophaga/fisiologia , Eikenella corrodens/fisiologia , Fusobacterium nucleatum/fisiologia , Humanos , Interações Microbianas , Viabilidade Microbiana , Porphyromonas gingivalis/fisiologia , Prevotella intermedia/fisiologia , Temperatura
11.
Crit Rev Oral Biol Med ; 15(4): 207-20, 2004 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-15284186

RESUMO

Motile bacteria often use sophisticated chemotaxis signaling systems to direct their movements. In general, bacterial chemotactic signal transduction pathways have three basic elements: (1) signal reception by bacterial chemoreceptors located on the membrane; (2) signal transduction to relay the signals from membrane receptors to the motor; and (3) signal adaptation to desensitize the initial signal input. The chemotaxis proteins involved in these signal transduction pathways have been identified and extensively studied, especially in the enterobacteria Escherichia coli and Salmonella enterica serovar typhimurium. Chemotaxis-guided bacterial movements enable bacteria to adapt better to their natural habitats via moving toward favorable conditions and away from hostile surroundings. A variety of oral microbes exhibits motility and chemotaxis, behaviors that may play important roles in bacterial survival and pathogenesis in the oral cavity.


Assuntos
Fenômenos Fisiológicos Bacterianos , Quimiotaxia/fisiologia , Boca/microbiologia , Periodontite/microbiologia , Proteínas de Bactérias , Campylobacter/patogenicidade , Campylobacter/fisiologia , Capnocytophaga/patogenicidade , Capnocytophaga/fisiologia , Células Quimiorreceptoras , Placa Dentária/microbiologia , Humanos , Receptores de Superfície Celular , Selenomonas/patogenicidade , Selenomonas/fisiologia , Transdução de Sinais , Treponema/patogenicidade , Treponema/fisiologia
12.
Oral Microbiol Immunol ; 18(5): 333-7, 2003 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-12930529

RESUMO

Streptococcus salivarius is divided into two serological subgroups that carry either fibrils or fimbriae. Although fimbriae have been observed on up to 50% of S. salivarius strains in the human oral cavity, no function has yet been assigned to them. To determine whether S. salivarius fimbriae have a role in adhesion, we examined the ability of S. salivarius to coaggregate with selected microorganisms involved in periodontal diseases. Our results show that S. salivarius coaggregated with Fusobacterium nucleatum, Porphyromonas gingivalis, and Prevotella intermedia. However, only fimbriated S. salivarius cells were able to coaggregate with P. intermedia, suggesting a specific role for these structures in the interaction. Heat treatment, sensitivity to sugars, amino acids, and EDTA, as well as protease treatment were also used to further characterize coaggregation between S. salivarius and periodontopathogens.


Assuntos
Aderência Bacteriana/fisiologia , Fímbrias Bacterianas/fisiologia , Prevotella intermedia/fisiologia , Streptococcus/fisiologia , Aggregatibacter actinomycetemcomitans/fisiologia , Aminoácidos/farmacologia , Aderência Bacteriana/efeitos dos fármacos , Capnocytophaga/fisiologia , Carboidratos/farmacologia , Quelantes/farmacologia , Ácido Edético/farmacologia , Endopeptidases/farmacologia , Eubacterium/fisiologia , Fímbrias Bacterianas/efeitos dos fármacos , Fusobacterium nucleatum/fisiologia , Temperatura Alta , Humanos , Doenças Periodontais/microbiologia , Porphyromonas/classificação , Porphyromonas/fisiologia , Porphyromonas gingivalis/fisiologia , Prevotella/classificação , Prevotella/fisiologia , Streptococcus/classificação , Streptococcus/efeitos dos fármacos
14.
J Formos Med Assoc ; 97(1): 44-8, 1998 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-9481064

RESUMO

Capnocytophaga has been recognized as an opportunistic pathogen causing systemic infections in immunocompromised individuals with granulocytopenia and oral ulceration. Treatment of Capnocytophaga infection is often empiric. We retrospectively analyzed the clinical features of all patients with Capnocytophaga bacteremia seen at the National Taiwan University Hospital between January 1981 and December 1996 and the antimicrobial susceptibility of the isolates recovered from these patients. All the patients had underlying diseases, namely neoplastic disease (9 patients), hyperthyroidism (1), and bronchiectasis and tetralogy of Fallot (1). The clinical features of these patients were primary bacteremia (10) and pneumonia (1). Nine patients had nosocomial bacteremia and 10 patients had monomicrobial bacteremia. None had septic shock. All the patients responded well to appropriate antimicrobial therapy and survived. All isolates were susceptible to amoxicillin-clavulanate, imipenem, ciprofloxacin, erythromycin, clindamycin, tetracycline, and chloramphenicol but resistant to aminoglycosides and sulfamethoxazole-trimethoprim. The susceptibilities to penicillin, ampicillin, piperacillin, cephalosporins, and aztreonam were variable. Capnocytophaga bacteremia should be included in the differential diagnosis of febrile neutropenia in immunocompromised patients, especially in the presence of oral mucositis and ulceration.


Assuntos
Bacteriemia/microbiologia , Capnocytophaga , Infecções por Bactérias Gram-Negativas/microbiologia , Adolescente , Adulto , Idoso , Bacteriemia/diagnóstico , Bacteriemia/epidemiologia , Capnocytophaga/isolamento & purificação , Capnocytophaga/fisiologia , Criança , Pré-Escolar , Resistência Microbiana a Medicamentos , Feminino , Infecções por Bactérias Gram-Negativas/diagnóstico , Infecções por Bactérias Gram-Negativas/epidemiologia , Humanos , Masculino , Pessoa de Meia-Idade , Neutropenia/microbiologia , Infecções Oportunistas/microbiologia , Estudos Retrospectivos , Taiwan/epidemiologia
15.
J Periodontal Res ; 32(6): 530-42, 1997 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-9379321

RESUMO

Information on intraoral distribution of putative periodontal pathogens might be essential for controlling different forms of periodontal disease. Colonization may be either promoted or impeded by other bacteria competing in the subgingival ecosystem. In recent investigations microbial associations between dental organisms have been determined in a multitude of subgingival plaque samples within multiple patients and described by odds ratios, in most circumstances without taking into account the correlated structure of the observations within a single individual. The present investigation had 3 major objectives: (i) to describe the intraoral distribution of some facultatively anaerobic, Gram-negative rods, i.e. Actinobacillus actinomycetemcomitans, Eikenella corrodens-like organisms and Capnocytophaga spp., in a multitude of subgingival and extracrevicular samples of 10 adult subjects with A. actinomycetemcomitans-associated periodontitis; (ii) to analyse possible inconsistencies of microbial associations between these periodontal organisms; and (iii) to determine factors increasing the likelihood of isolating these bacteria in a given subgingival site by employing Generalized Estimation Equation (GEE) methods. Clinical examinations were carried out at 6 sites of every tooth present. In each subject, 13 extracrevicular (2 cheek mucosa, 3 tongue, 4 gingival, 2 tonsillar samples, 1 palatinal, 1 saliva sample) and between 22 and 44 subgingival samples from deepest sites of every tooth present (n = 296) were selectively cultivated for A. actinomycetemcomitans, E. corrodens and Capnocytophaga spp. In extracrevicular material, A. actinomycetemcomitans, Capnocytophaga spp. and E. corrodens were isolated in 9, 10 and 6 patients, and from 65, 82 and 15% samples, respectively. The organisms were recovered from 51, 62 and 27% subgingival plaque samples, respectively. Heterogeneity tests did not reveal significant inconsistencies of microbial associations between bacteria in subgingival plaque. Mantel-Haenszel's odds ratios ranged between 2.0 for A. actinomycetemcomitans and Capnocytophaga spp. and 18.7 for Capnocytophaga spp. and E. corrodens. An exchangeable working dependence structure was employed in the GEE approach. The odds of isolating A. actinomycetemcomitans was increased by factor 3.7 in 4-6 mm deep pockets, and 9.5 in > or = 7 mm deep pockets. The odds of presence of E. corrodens was increased by factor 10.8 in the case of presence of Capnocytophaga spp. and 2.1 in the case of presence of A. actinomycetemcomitans. Capnocytophaga spp. were associated with bleeding on probing and molar sites. Presence of E. corrodens was associated with clinical attachment loss but not periodontal probing depth. Results of the present study indicated an association of A. actinomycetemcomitans with periodontal pathology. Whereas this organism and Capnocytophagae were widely distributed in extracrevicular ecosystems of the mouth, E. corrodens only occasionally appeared in saliva or on mucous membranes of the oral cavity. In general, GEE methods seem to allow to determine factors associated with the presence of periodontal organisms in a multivariate approach and considering the correlated structure of the data.


Assuntos
Aggregatibacter actinomycetemcomitans/fisiologia , Capnocytophaga/fisiologia , Eikenella corrodens/fisiologia , Periodontite/microbiologia , Adulto , Bochecha/microbiologia , Placa Dentária/microbiologia , Ecologia , Ecossistema , Feminino , Gengiva/microbiologia , Hemorragia Gengival/microbiologia , Humanos , Funções Verossimilhança , Masculino , Pessoa de Meia-Idade , Dente Molar/microbiologia , Mucosa Bucal/microbiologia , Análise Multivariada , Razão de Chances , Palato/microbiologia , Tonsila Palatina/microbiologia , Perda da Inserção Periodontal/microbiologia , Bolsa Periodontal/microbiologia , Saliva/microbiologia , Língua/microbiologia
16.
Bull Tokyo Dent Coll ; 35(4): 171-81, 1994 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-8689730

RESUMO

To elucidate an ecological profile of several periodontopathogens, the authors examined the coaggregation between cells of Porphyromonas gingivalis and oral bacterial strains including Treponema denticola in vitro. Coaggregation between cells of plaque bacteria was examined by visual assay and phase-contrast microscope. P. gingivalis cells coaggregated with strains of T. denticola and Treponema socranskii subspecies socranskii, but did not coaggregate with T. socranskii subspecies buccale, T. socranskii subspecies paredis, Treponema vincentii, or Treponema pectinovorum. The extracted hemagglutinin from P. gingivalis was active agglutinating T. denticola cells. Addition of serum and saliva somewhat affected the coaggregation, but no effects of tested sugars or amino acids were found. Heat treatment of T. denticola cells did not reduce the coagregation: heat treatment of P. gingivalis cells eliminated it. Growth inhibitory activity among these bacterial species was examined by the stab culture method. Strains of T. denticola ATCC 35404 and 35405 and T. vincentii inhibited the growth of some P. gingivalis strains, but not others. No strain of Treponema was inhibited by black-pigmented anaerobic rods. The coaggregation observed between P. gingivalis and T. denticola indicates the potential importance of their simultaneous existence in human periodontal pockets and development of the disease.


Assuntos
Aderência Bacteriana/fisiologia , Porphyromonas gingivalis/fisiologia , Treponema/fisiologia , Actinomyces/efeitos dos fármacos , Actinomyces/fisiologia , Aminoácidos/farmacologia , Antibiose , Aderência Bacteriana/efeitos dos fármacos , Capnocytophaga/efeitos dos fármacos , Capnocytophaga/fisiologia , Carboidratos/farmacologia , Placa Dentária/microbiologia , Ecossistema , Ácido Edético/farmacologia , Eikenella corrodens/efeitos dos fármacos , Eikenella corrodens/fisiologia , Endopeptidases/farmacologia , Temperatura Alta , Humanos , Periodontite/microbiologia , Porphyromonas gingivalis/química , Porphyromonas gingivalis/efeitos dos fármacos , Saliva/fisiologia , Especificidade da Espécie , Streptococcus/efeitos dos fármacos , Streptococcus/fisiologia , Simbiose , Treponema/efeitos dos fármacos
17.
Arch Oral Biol ; 37(9): 717-24, 1992 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-1329700

RESUMO

Porphyromonas gingivalis, an important pathogen in periodontitis, produces extracellular vesicles that aggregate with Actinomyces viscosus cells. A 40-kDa outer membrane protein (OMP)-coding gene from P. gingivalis was cloned and the protein was found to be localized in these vesicles. The recombinant 40-kDa OMP did not show aggregation activity. However, affinity-purified antibody against the recombinant protein significantly inhibited aggregation of P. gingivalis vesicles with A. viscosus cells. The antibody also inhibited cellular coaggregation of several strains of P. gingivalis with A. viscosus cells, but not with other periodontal pathogens. Moreover, aggregation of A. viscosus cells with P. gingivalis vesicles was inhibited in a dose-dependent manner by pre-treatment of the A. viscosus cells with the recombinant protein. These findings suggest that the 40-kDa OMP may be an important aggregation factor of P. gingivalis.


Assuntos
Actinomyces viscosus/fisiologia , Aderência Bacteriana/fisiologia , Proteínas da Membrana Bacteriana Externa/farmacologia , Porphyromonas gingivalis/fisiologia , Proteínas da Membrana Bacteriana Externa/análise , Capnocytophaga/fisiologia , Membrana Celular/fisiologia , Membrana Celular/ultraestrutura , Eikenella corrodens/fisiologia , Fusobacterium nucleatum/fisiologia , Peso Molecular , Porphyromonas gingivalis/metabolismo , Porphyromonas gingivalis/ultraestrutura , Vacúolos/fisiologia , Vacúolos/ultraestrutura
18.
J Clin Periodontol ; 19(6): 417-22, 1992 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-1321846

RESUMO

The present investigation examined the relationship of selected bacterial species and subgingival temperature. 35 subjects were measured at 6 sites per tooth for clinical parameters and subgingival temperature. Measurements were repeated for 21 subjects at 2 month intervals providing a total of 66 subjects visits. At each visit, subgingival plaque samples were taken from the mesial aspect of each tooth and anaerobically dispersed, diluted and plated on non-selective media. After anaerobic incubation, colonies were lifted to nylon filters and specific species detected using digoxigenin-labeled whole chromosomal DNA probes. Species enumerated were; A. actinomycetemcomitans serotypes a and b, B. forsythus, B. gingivalis, B. intermedius I and II, C. ochracea, F. nucleatum ss. vincentii, P. micros, S. intermedius, S. sanguis I and II, V. parvula and W. recta. Total viable counts and counts of Capnocytophaga sp. were determined directly from the primary isolation plates. A total of 1581 samples were evaluated. Subject visits with higher mean subgingival temperatures had significantly higher mean %s of B. intermedius I and P. micros, and lower mean %s of Capnocytophaga sp. Sites with higher subgingival temperatures had elevated proportions of B. intermedius I and II, A. actinomycetemcomitans serotype a and B. gingivalis more frequently than sites with lower temperatures, while Capnocytophaga sp. were elevated more often at cooler sites. 43 of the subject visits had follow up attachment level measurements at 2 months. The 1026 microbial samples and the subgingival temperature measurements from these visits were related to longitudinal attachment change.(ABSTRACT TRUNCATED AT 250 WORDS)


Assuntos
Fenômenos Fisiológicos Bacterianos , Temperatura Corporal/fisiologia , Gengiva/fisiologia , Adolescente , Adulto , Idoso , Aggregatibacter actinomycetemcomitans/fisiologia , Bacteroides/fisiologia , Capnocytophaga/fisiologia , Contagem de Colônia Microbiana , Placa Dentária/microbiologia , Análise Discriminante , Feminino , Fusobacterium nucleatum/fisiologia , Gengiva/microbiologia , Humanos , Masculino , Pessoa de Meia-Idade , Peptostreptococcus/fisiologia , Doenças Periodontais/microbiologia , Doenças Periodontais/fisiopatologia , Porphyromonas gingivalis/fisiologia , Análise de Regressão , Fatores de Risco , Streptococcus/fisiologia , Streptococcus sanguis/fisiologia , Veillonella/fisiologia , Wolinella/fisiologia
19.
Oral Microbiol Immunol ; 7(2): 96-9, 1992 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-1528638

RESUMO

A bacteriocin from a subgingival plaque isolate of Capnocytophaga ochracea was purified and characterized. It was isolated from cell extract and had a molecular weight of 100,000. Streptococcus sanguis, Streptococcus mitis, Streptococcus mutans and Propionibacterium acnes were susceptible to the bacteriocin.


Assuntos
Antibiose , Bacteriocinas/isolamento & purificação , Bacteriocinas/farmacologia , Capnocytophaga/fisiologia , Boca/microbiologia , Actinomyces/efeitos dos fármacos , Bacteriocinas/química , Bacteroides/efeitos dos fármacos , Ecologia , Eletroforese em Gel de Poliacrilamida , Fusobacterium nucleatum/efeitos dos fármacos , Lacticaseibacillus casei/efeitos dos fármacos , Propionibacterium/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacos , Streptococcus/efeitos dos fármacos
20.
Aust Dent J ; 37(2): 121-5, 1992 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-1605750

RESUMO

Polymorphonuclear neutrophils (PMNs) comprise over 90 per cent of leukocytes in the oral cavity. Although these phagocytic cells have primary defence roles in the gingiva, their stimulation by micro-organisms may also cause substantial tissue damage due to the release of lysosomal enzymes and oxygen radicals. Adherence of PMNs to the endothelium and their subsequent diapedesis and egress to areas of infection are considered early vital events in the inflammatory process. In this study, oral bacteria were screened to determine their direct effects on PMN activation using an in vitro method of measuring PMN adherence to Dacron fibres. Most of the bacteria investigated increased PMN adherence, indicating their potential to cause tissue damage through the release of PMN lysosomal enzymes and other products. In contrast, Bacteroides species suppressed PMNs, indicating their ability to circumvent the phagocytic cells, thus gaining a potential advantage in dental colonization. The modulatory effects of oral bacteria on PMN activation may have significant roles in the immunopathogenesis of oral disease.


Assuntos
Fenômenos Fisiológicos Bacterianos , Neutrófilos/fisiologia , Aggregatibacter actinomycetemcomitans/fisiologia , Bacteroides/fisiologia , Capnocytophaga/fisiologia , Adesão Celular , Eikenella corrodens/fisiologia , Fusobacterium/fisiologia , Humanos , Lactobacillus/fisiologia , Lacticaseibacillus casei/fisiologia , Neutrófilos/citologia , Peptostreptococcus/fisiologia , Streptococcus/fisiologia , Streptococcus sanguis/fisiologia , Veillonella/fisiologia , Wolinella/fisiologia
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