RESUMO
INTRODUCTION: On a farm in the Kyrgyz Republic, several dead sheep were found without any history of illness. The sheep showed several ulcerations on lips and bare-skinned areas. At necropsy the lungs showed multiple firm nodules, which were defined as pox nodules histologically. In the rumen hyperkeratotic plaques were visible. With electron microscopy pox viral particles were detected and confirmed with q PCR as Capripoxviruses. Although all members of the Capripoxvirus genus are eradicated in western countries, this study should remind us of the classical lesions observed in poxvirus infections.
Assuntos
Capripoxvirus/genética , Capripoxvirus/ultraestrutura , Infecções por Poxviridae/veterinária , Doenças dos Ovinos/patologia , Doenças dos Ovinos/virologia , Animais , Capripoxvirus/isolamento & purificação , Quirguistão , Lábio/patologia , Pulmão/patologia , Microscopia Eletrônica de Transmissão , Infecções por Poxviridae/patologia , Infecções por Poxviridae/virologia , Rúmen/patologia , OvinosRESUMO
An outbreak of sheep pox was investigated in the Ningxia Hui Autonomous Region in China. Through immunofluorescence testing, isolated viruses, polymerase chain reaction identification, and electron microscopic examination, the isolated strain was identified as a sheep pox virus. The virus was identified through sequence and phylogenetic analysis of the P32 gene, open reading frame (ORF) 095, and ORF 103 genes. This study is the first to use the ORF 095 and ORF 103 genes as candidate genes for the analysis of sheep pox. The results showed that the ORF 095 and ORF 103 genes could be used for the genotyping of the sheep pox virus.
Assuntos
Capripoxvirus/classificação , Capripoxvirus/isolamento & purificação , Filogenia , Animais , Sequência de Bases , Capripoxvirus/ultraestrutura , China , Masculino , Microscopia de Fluorescência , Infecções por Poxviridae/patologia , Infecções por Poxviridae/veterinária , Infecções por Poxviridae/virologia , Ovinos , Doenças dos Ovinos/virologia , Pele/patologia , Pele/virologia , Testículo/patologia , Testículo/virologia , Vírion/ultraestruturaRESUMO
The present study describes the detection of sheeppox virus antigen in various lamb tissues, using an immunohistochemical technique, in sheeppox cases which occurred naturally. Sheeppox viral antigen was detected in the cytoplasm of sheeppox cells and degenerated epithelial cells of the skin, lungs and digestive tract involving typical sheeppox lesions. Nuclear staining was also observed in some typically deformed nuclei of sheeppox cells. The immunostaining of sheeppox virus showed a correlation with the presence of sheeppox cells and degenerated epithelial cells resembling them. Additionally, in order to confirm the presence of sheeppox virus in the skin and lung samples, direct electron microscopy was performed and sheeppox virus was only demonstrated in two skin samples.
