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1.
Food Environ Virol ; 13(1): 64-73, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33165867

RESUMO

Cosaviruses (CoSV) and Saffold cardiovirus (SAFV) are novel members of the Picornaviridae family. The Matanza-Riachuelo river basin covers a total area of 2200 km2 with approximately 60 km long. Its last section is called Riachuelo River. The aim of this study was to describe the circulation of both picornaviruses and their relationship with the environmental situation of the Riachuelo River using 274 samples collected from 2005 to 2015. CoSV and SAFV were investigated in samples available by two periods: 2005-2006 and 2014-2015 (103 and 101, respectively). Physicochemical and bacteriological parameters confirmed very high levels of human fecal contamination during the 11 years evaluated. CoSV was detected in 85.7% (66/77) and 65.4% (17/26) of the samples collected in 2005-2006 and 2014-2015 periods, respectively. Species A and D were identified, the first one being widely predominant: 74.1% (20/27) and 75.0% (3/4) in both periods. SAFV virus was detected in 47.1% (32/68) and 52.6% (10/19) in periods 2005-2006 and 2014-2015, respectively. SAFV-6 was the most identified genotype in the entire study, while SAFV-3 was predominant in 2005-2006. The contribution of genotypes 1, 2, 4 and 8 was minor. The high prevalence of CoSV and SAFV suggests that both viruses have been circulating in Argentina at least since 2005. Our results show that a watercourse with high rates of human fecal contamination can become a persistent source of new viruses which capacity to produce human diseases is unknown.


Assuntos
Cardiovirus/isolamento & purificação , Picornaviridae/isolamento & purificação , Rios/virologia , Argentina , Cardiovirus/classificação , Cardiovirus/genética , Fezes/virologia , Genótipo , Humanos , Filogenia , Picornaviridae/classificação , Picornaviridae/genética , Poluição da Água/análise
2.
J Med Virol ; 93(6): 3980-3984, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-32827319

RESUMO

Viral gastroenteritis is a major source of morbidity and mortality, predominantly caused by so-called NOROAD viruses (norovirus, rotavirus, and adenovirus). In approximately onethird of all cases, however, the exact etiology is unknown. The in 2007 discovered human cardiovirus Saffold virus (SAFV) may prove to be a plausible candidate to explain this diagnostic gap. This virus, a member of the Picornaviridae family which is closely related to the murine viruses Theiler's murine encephalomyelitis virus and Theravirus, is a widespread pathogen and causes infection early in life. Screening of 238 fecal or vomitus samples obtained from NOROAD-negative, elderly patients with acute gastroenteritis at the University Hospital of Linköping showed that SAFV is present in low abundance (4.6%). Phylogenetic analysis of the VP1 gene revealed a Swedish isolate belonging to the highly common and in Europe widespread SAFV-3 genotype. This genotype is also related to previously reported Asian strains. This study describes the first molecular typing of a Swedish SAFV isolate and is the first report to document the circulation of SAFV among elderly people. The pathogenicity of SAFV is, as of yet, still under debate; further studies are necessary to determine its role in the development of disease.


Assuntos
Infecções por Cardiovirus/epidemiologia , Cardiovirus/classificação , Cardiovirus/genética , Gastroenterite/epidemiologia , Gastroenterite/virologia , Doença Aguda/epidemiologia , Idoso , Idoso de 80 Anos ou mais , Cardiovirus/patogenicidade , Infecções por Cardiovirus/virologia , Fezes/virologia , Genoma Viral , Genótipo , Humanos , Filogenia , Suécia/epidemiologia
3.
Lett Appl Microbiol ; 70(2): 102-108, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31742735

RESUMO

Saffold virus (SAFV) is an emerging human cardiovirus associated with respiratory and gastrointestinal infection, and, more recently, to symptoms related to the endocrine, cardiovascular, and neurological systems. Information about SAFV circulation in Italy is scarce. In order to provide insights into the epidemiology of SAFV in Italy, 141 raw sewage samples collected throughout Italy were tested using broad-range nested RT-PCR primers targeting the 5'-NC region. Seven samples (5·0%) were confirmed as SAFV in samples collected in North, Centre and Southern Italy. Typing was attempted through amplification of the VP1 coding region, using both published and newly designed primers, and one sample was characterized as SAFV-2. SIGNIFICANCE AND IMPACT OF THE STUDY: Prevalence, genetic diversity and geographic distribution of SAFV in Italy is currently unknown. This study represents the first detection of SAFV in sewage samples in Italy, suggesting that it is circulating in the population despite lack of clinical reporting. Whether the virus is associated with asymptomatic cases or with undetected gastroenteritis or respiratory illness is unknown. Further studies are needed to investigate on the occurrence and persistence of SAFV in water environments and its waterborne transmission potential.


Assuntos
Infecções por Cardiovirus/epidemiologia , Cardiovirus/isolamento & purificação , Gastroenteropatias/epidemiologia , Infecções Respiratórias/epidemiologia , Esgotos/virologia , Proteínas do Capsídeo/genética , Cardiovirus/classificação , Cardiovirus/genética , Infecções por Cardiovirus/virologia , Monitoramento Ambiental , Gastroenteropatias/virologia , Humanos , Itália/epidemiologia , Prevalência , Infecções Respiratórias/virologia
4.
Food Environ Virol ; 12(1): 75-80, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-31729639

RESUMO

Saffold virus as a newly discovered virus, which seems to be related to acute gastroenteritis as with other enteric viruses and to human airway diseases in children belongs to Cardiovirus genus in picornaviridae family with 11 genotypes. Saffold virus initially was detected in America from infant stool sample. Saffold virus has also been detected in environmental water samples. Until now, two reports have demonstrated that sewage water sources are contaminated with Saffold viruses. Molecular detection of Saffold virus mostly depended on reverse transcription PCR methods and RT-qPCR, which had targeted 5'UTR region of the viral genome. The present study aims to evaluate the molecular detection and quantity of Saffold virus in sewage water and river water specimens by RT-qPCR assay in Karaj, Iran. Fifty samples collected from environmental waters containing treated and untreated sewage water and river water samples were included in this study. After viral RNA extraction, the Real-time PCR was developed to amplify the 5'UTR sequence of Saffold virus genome and viral load was assessed. Out of the 50 samples tested (consisting 28 river water samples and 22 sewage water samples), the Saffold virus genomic RNA was identified in 10/28 (35.7%) of river water samples and in 4/12 (33.3%) of treated and 4/10 (40%) of untreated sewage samples. The maximum viral load was 6.8 × 106 copies/l in untreated sewage water sample in December, and the lower viral load was 1.2 × 106 copies/l related to treated sewage water taken in October. Our results for the first time indicate that Saffold virus has apparently been circulating among Iranian peoples. Also, the viral prevalence of Saffold virus in each of the three sets of tested samples was within moderate to high in range.


Assuntos
Cardiovirus/isolamento & purificação , Rios/virologia , Esgotos/virologia , Cardiovirus/classificação , Cardiovirus/genética , Gastroenterite/virologia , Genoma Viral , Genótipo , Humanos , Irã (Geográfico) , Reação em Cadeia da Polimerase em Tempo Real
5.
Intervirology ; 62(1): 45-50, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31207600

RESUMO

BACKGROUND/AIMS: Cardiovirus is a genus of viruses belonging to the family Picornaviridae. Here, we used viral metagenomic techniques to detect the viral nucleic acid in the fecal samples from wild rats in Zhenjiang city in China. METHOD: Fecal samples were collected from 20 wild rats and pooled into four sample pools and then subjected to libraries construction which were then sequenced on Illumina MiSeq platform. The sequenced reads were analyzed using viral metagenomic analysis pipeline. RESULTS: A novel cardiovirus from feces of a wild rat was identified, named amzj-2018, of which the complete genome was acquired. Phylogenetic analysis based on the complete amino acid sequence of polyprotein revealed that amzj-2018 formed a separate branch located between clusters of Saffold virus and Rat Theilovirus 1 (RTV-1). Phylogenetic analysis based on different regions of the polyproteins, including P1, P2, P3, and P2+P3, respectively, showed discordant trees, where the tree based on P3 region indicated that amzj-2018 clustered separately between Theiler's murine encephalomyelitis virus and RTV-1. CONCLUSION: The complete genome of a cardiovirus was determined from the feces of wild rats which belonged to a novel type of cardiovirus based on phylogenetic analysis. Whether it is associated with disease needs further investigation.


Assuntos
Infecções por Cardiovirus/veterinária , Cardiovirus/classificação , Fezes/virologia , Metagenômica , Ratos/virologia , Animais , Cardiovirus/isolamento & purificação , China , Cidades , Genoma Viral , Filogenia , RNA Viral/genética , Proteínas Virais/genética
6.
Pediatr Infect Dis J ; 38(8): 781-785, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-30908431

RESUMO

BACKGROUND: Saffold virus (SAFV) is a novel human cardiovirus that was identified in 2007. Recently, SAFV has been isolated from nasal and stool specimens of infants presenting with respiratory and gastrointestinal symptoms and from cerebrospinal fluid (CSF) specimens of children with central nervous system infection. However, little is known regarding clinical characteristics of SAFV in children. METHODS: We reviewed 5412 specimens from the database of the infectious agents surveillance system in Niigata prefecture, Japan, between January 2006 and December 2013, and identified SAFV-infected patients. Subsequently, we retrospectively reviewed their medical records and evaluated their clinical characteristics. RESULTS: We identified 9 SAFV-infected patients (median age: 5 years; range: 2-16 years). Seven patients were diagnosed with pharyngitis, one with meningitis and one with fever of unknown origin. Dominant symptoms were high fever, appetite loss and headache. The median duration of the fevers was 2 days in patients with pharyngitis; however, the patient with meningitis remained febrile for 5 days. All blood tests available in this case series revealed leukocytosis with a predominance of neutrophils. CSF profiles showed mild lymphocytic pleocytosis. All patients recovered fully without complications. CONCLUSIONS: A few clinical characteristics of SAFV infection were clarified, including high fever of short duration in patients with pharyngitis, and neutrophil-dominant leukocytosis. The clinical course and CSF profiles of a case of meningitis were similar to those of other aseptic meningitis. SAFV needs to be included in the differential diagnosis of pharyngitis or meningitis when commonly identified viruses are not identified in such patients.


Assuntos
Infecções por Cardiovirus/diagnóstico , Infecções por Cardiovirus/virologia , Cardiovirus , Adolescente , Biomarcadores , Cardiovirus/classificação , Cardiovirus/genética , Infecções por Cardiovirus/epidemiologia , Criança , Pré-Escolar , Feminino , Genótipo , Humanos , Japão , Masculino , Meningite Viral/diagnóstico , Meningite Viral/virologia , Filogenia , Vigilância da População , Estudos Retrospectivos , Avaliação de Sintomas
7.
Virol J ; 15(1): 58, 2018 03 27.
Artigo em Inglês | MEDLINE | ID: mdl-29587779

RESUMO

BACKGROUND: Cardioviruses cause severe illnesses in rodents and humans. In recent years, novel cardioviruses have been frequently found, which promoted further studies of the genetic diversity of cardioviruses. Using viral metagenomics, we genetically characterized a novel cardiovirus (named SX1) from wild rat feces. The genomic structure of SX1 shared similar features with those of the Theiler's murine encephalomyelitis viruses, including a leader protein, four structural proteins and seven non-structural proteins. Phylogenetic analysis based on both structural proteins and non-structural proteins coding regions showed that SX1 was formed into a separate branch, being located between the branches of Theiler's murine encephalomyelitis viruses and Thera viruses. Variable resides presented in the Ser/Thr rich domain of L protein, VP1 loops, and VP2 puffs distinguished SX1 from Theiler's murine encephalomyelitis viruses, suggesting the different antigenicity and pathogenicity of SX1.


Assuntos
Cardiovirus/classificação , Cardiovirus/genética , Filogenia , Animais , Animais Selvagens/virologia , China , Fezes/virologia , Genoma Viral/genética , Metagenômica , Ratos , Alinhamento de Sequência , Especificidade da Espécie , Proteínas Virais/genética
8.
J Clin Virol ; 70: 7-13, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26305811

RESUMO

BACKGROUND: Saffold cardiovirus (SAFV) belongs to the Cardiovirus genus of Picornaviridae family, and may be a relevant new human pathogen; Thus far, eleven genotypes have been identified. The SAFV type 3 (SAFV-3) is thought to be the major genotype and is detected relatively frequently in children with acute gastroenteritis and respiratory illness. The epidemiology and pathogenicity of SAFV-3 remain unclear. OBJECTIVES: To investigate the genomic and epidemiologic profiles of SAFV-3 infection in Taiwan. STUDY DESIGN: Virus was detected in respiratory samples from children suffering for URI. SAFV-3 isolates were detected by isolation on cell culture and IF assay. The molecular typing was performed by RT-PCR and was sequenced to compare with reference strains available in the NCBI GeneBank. Serum samples were collected from 2005 to 2013 in Taiwan for seroprevalence investigation. RESULTS: A total of 226 specimens collected from children with URIs, 22 (9.73%) were positive for SAFV-3. The majority of SAFV-3 infections were found in children less than 6 years of age (14 of 22, 63.6%). Genetic analysis of VP1 coding region of Taiwanese isolates shown an 83.2-97.7% difference from other available SAFV-3 sequences in NCBI GenBank. Phylogenetic analysis revealed there is three genetic groups of SAFV-3 co-circulated in Taiwan during the study period. In addition, seroprevalence investigation results indicated that SAFV-3 infection occurs early in life and 43.7-77.8% of children aged between 6 months to 9 years old, had neutralizing antibodies against SAFV-3. CONCLUSION: SAFV-3 may have circulated in Taiwan for some time and it appears to be one of the etiological agents responsible for URIs in children.


Assuntos
Infecções por Cardiovirus/epidemiologia , Infecções por Cardiovirus/virologia , Cardiovirus/genética , Genótipo , Infecções Respiratórias/epidemiologia , Infecções Respiratórias/virologia , Adolescente , Anticorpos Neutralizantes/sangue , Anticorpos Neutralizantes/imunologia , Anticorpos Antivirais/sangue , Anticorpos Antivirais/imunologia , Cardiovirus/classificação , Cardiovirus/imunologia , Cardiovirus/isolamento & purificação , Infecções por Cardiovirus/diagnóstico , Linhagem Celular , Criança , Pré-Escolar , Feminino , Variação Genética , Genoma Viral , Humanos , Lactente , Recém-Nascido , Masculino , Filogenia , Prevalência , Infecções Respiratórias/diagnóstico , Estudos Soroepidemiológicos , Taiwan/epidemiologia
9.
Sci Rep ; 5: 7704, 2015 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-25572936

RESUMO

The epidemiology and clinical features of the Saffold cardiovirus (SAFV) remain ambiguous. The present study was designed to systematically and intensively investigate the epidemiological features of SAFV in pediatric patients in China. Three cohorts of pediatric patients were recruited from 2009 to 2012. Cohort 1 comprised patients with acute respiratory tract infections. Cohort 2 comprised patients with diarrhea. Cohort 3 comprised hand, foot, and mouth disease (HFMD) patients. A total of 115 patients (1.6%) among 6052 (17/1647, 12/2013, and 86/2392 in cohorts 1, 2, and 3, respectively) were SAFV-positive. The samples from 82 SAFV-positive patients were successfully sequenced, and four genotypes were identified: 8 SAFV-1, 41 SAFV-2, 29 SAFV-3, and 4 SAFV-6. A significantly higher detection rate was found in the HFMD patients than in other two cohorts (both P <0.001). A higher frequency of severe clinical outcome and nervous system manifestation were also observed in the SAFV-positive HFMD patients. Additionally, 6 (3.5%) cerebrospinal fluid and 7 (2.2%) serum samples from the HFMD-associated encephalitis patients were SAFV-positive. Based on the VP1 sequences, all four genotypes displayed distinct geographical clustering. SAFV infection might be associated with a wide clinical spectrum and contribute to HFMD.


Assuntos
Infecções por Cardiovirus/epidemiologia , Cardiovirus/genética , Adolescente , Sequência de Bases , Proteínas do Capsídeo/genética , Cardiovirus/classificação , Cardiovirus/isolamento & purificação , Infecções por Cardiovirus/complicações , Infecções por Cardiovirus/virologia , Criança , Pré-Escolar , China/epidemiologia , Estudos de Coortes , Diarreia/complicações , Diarreia/virologia , Feminino , Genótipo , Doença de Mão, Pé e Boca/complicações , Doença de Mão, Pé e Boca/virologia , Humanos , Lactente , Masculino , Filogenia , Prevalência , RNA Viral/análise , Reação em Cadeia da Polimerase em Tempo Real , Infecções Respiratórias/complicações , Infecções Respiratórias/virologia , Sorotipagem
10.
J Gen Virol ; 95(Pt 9): 1945-1957, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24899154

RESUMO

Human cardioviruses or Saffold viruses (SAFVs) of the family Picornaviridae are newly emerging viruses whose genetic and phenotypic diversity are poorly understood. We report here the full genome sequence of 11 SAFV genotypes from Pakistan and Afghanistan, along with a re-evaluation of their genetic diversity and recombination. We detected 88 SAFV from stool samples of 943 acute flaccid paralysis cases using reverse transcriptase-PCR targeting the 5' untranslated region (UTR). Further characterization based on complete VP1 analysis revealed 71 SAFVs belonging to 11 genotypes, including three previously unidentified genotypes. SAFV showed high genetic diversity and recombination based on phylogenetic, pairwise distance distributions and recombination mapping analyses performed herein. Phylogenies based on non-structural and UTRs were highly incongruent indicating frequent recombination events among SAFVs. We improved the SAFV genotyping classification criteria by determining new VP1 thresholds based on the principles used for the classification of enteroviruses. For genotype assignment, we propose a threshold of 23 and 10 % divergence for VP1 nucleotide and amino acid sequences, respectively. Other members of the species Theilovirus, such as Thera virus and Theiler's murine encephalomyelitis virus, are difficult to classify in the same species as SAFV, because they are genetically distinct from SAFV, with 41-56 % aa pairwise distances. The new genetic information obtained in this study will improve our understanding of the evolution and classification of SAFV.


Assuntos
Cardiovirus/classificação , Cardiovirus/genética , Genoma Viral/genética , Proteínas Virais/genética , Regiões 5' não Traduzidas/genética , Afeganistão , Sequência de Aminoácidos , Sequência de Bases , Evolução Biológica , Proteínas do Capsídeo/genética , Infecções por Cardiovirus/virologia , Mapeamento Cromossômico , Fezes/virologia , Variação Genética , Genótipo , Humanos , Dados de Sequência Molecular , Hipotonia Muscular/virologia , Paquistão , Fenótipo , Análise de Sequência de RNA , Theilovirus/genética
11.
Infect Genet Evol ; 13: 339-43, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-23183311

RESUMO

Saffold virus (SAFV) is a newly discovered human virus in the genus Cardiovirus, family Picornaviridae. The virus was first described from fecal specimens of a child with fever of unknown origin in 2007. A total of 454 fecal specimens were collected from children with diarrhea attended clinics in Japan, 2010-2011, 7 (1.5%) were positive for SAFV. Mixed-infections of SAFV and other enteric viruses (rotavirus, norovirus, and bocavirus) were found in four out of seven cases, while monoinfection by SAFV alone was detected in three cases. In addition to diarrhea, fever and vomiting were observed in three children and mild dehydration in one case. No particular symptoms of cough and rhinorrhea were noted. Analysis of partial VP1 nucleotide sequence of 7 Japanese SAFV strains revealed that 5 SAFV sequences were most closely related with SAFV2 reference strains, but separated into SAFV2-A (3 strains) and SAFV2-B (2 strains). In addition, the other two strains were classified as SAFV3. Our results indicated that SAFVs (SAFV2 and SAFV3) were circulated in children with acute gastroenteritis in Japan during 2010 and 2011 epidemic season.


Assuntos
Infecções por Cardiovirus/virologia , Cardiovirus/genética , Diarreia/virologia , Proteínas do Capsídeo/genética , Cardiovirus/classificação , Infecções por Cardiovirus/epidemiologia , Criança , Pré-Escolar , Diarreia/epidemiologia , Feminino , Genótipo , Humanos , Lactente , Japão/epidemiologia , Masculino , Dados de Sequência Molecular , Filogenia
13.
Emerg Infect Dis ; 18(1): 7-12, 2012 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22261113

RESUMO

The first human virus in the genus Cardiovirus was described in 2007 and named Saffold virus (SAFV). Cardioviruses can cause severe infections of the myocardium and central nervous system in animals, but SAFV has not yet been convincingly associated with disease in humans. To study a possible association between SAFV and infections in the human central nervous system, we designed a real-time PCR for SAFV and tested cerebrospinal fluid (CSF) samples from children <4 years of age. SAFV was detected in 2 children: in the CSF and a fecal sample from 1 child with monosymptomatic ataxia caused by cerebellitis; and in the CSF, blood, and myocardium of another child who died suddenly with no history of illness. Virus from each child was sequenced and shown to be SAFV type 2. These findings demonstrate that SAFV can cause serious invasive infection in children.


Assuntos
Infecções por Cardiovirus/patologia , Cardiovirus/isolamento & purificação , Infecções do Sistema Nervoso Central/virologia , Cardiovirus/classificação , Cardiovirus/genética , Infecções por Cardiovirus/líquido cefalorraquidiano , Infecções do Sistema Nervoso Central/patologia , Pré-Escolar , Evolução Fatal , Fezes/virologia , Feminino , Genoma Viral , Humanos , Lactente , Masculino , Filogenia , Reação em Cadeia da Polimerase em Tempo Real
14.
Emerg Infect Dis ; 17(12): 2313-5, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22153118

RESUMO

Cardioviruses cause myocarditis and encephalomyelitis in rodents; human cardioviruses have not been ascribed to any disease. We screened 6,854 cerebrospinal fluid and 10 myocardium specimens from children and adults. A genotype 2 cardiovirus was detected from a child who died of sudden infant death syndrome, and 2 untypeable cardioviruses were detected from 2 children with meningitis.


Assuntos
Infecções por Cardiovirus/virologia , Meningite Viral/virologia , Morte Súbita do Lactente/líquido cefalorraquidiano , Adulto , Cardiovirus/classificação , Cardiovirus/genética , Cardiovirus/isolamento & purificação , Infecções por Cardiovirus/líquido cefalorraquidiano , Criança , Estudos de Coortes , Doenças Transmissíveis Emergentes/líquido cefalorraquidiano , Doenças Transmissíveis Emergentes/virologia , Alemanha , Humanos , Lactente , Meningite Viral/líquido cefalorraquidiano , Miocardite/líquido cefalorraquidiano , Miocardite/virologia , Filogenia , RNA Viral/líquido cefalorraquidiano , RNA Viral/genética
16.
Pediatr Infect Dis J ; 30(8): 680-3, 2011 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-21386746

RESUMO

BACKGROUND: Saffold virus (SAFV) is a newly discovered virus belonging to the genus Cardiovirus of the family Picornaviridae. Using molecular techniques, SAFV has been detected, although infrequently, in the stools of both healthy and diarrheic children and in respiratory specimens collected from children with respiratory disease. The epidemiology and pathogenicity of SAFV remain unclear. METHODS: Between July 2009 and October 2010, nasopharyngeal specimens were collected from children with acute respiratory infections. The collected samples were used to isolate respiratory viruses, including coxsackievirus, by cell culture and were tested for SAFV by reverse transcription-polymerase chain reaction. RESULTS: SAFV genotype 2 (SAFV2) was detected in 54 (3.5%) of the 1525 children tested. SAFV2 detections showed an epidemic pattern for a 4-month period with a peak in October 2009. The median age of the SAFV2-positive children was 4 years (range: 7 months-16 years). Among the 35 SAFV2-positive children, excluding cases of viral coinfection, 13 (37.1%) had pharyngitis, 12 (34.3%) had tonsillitis, and 8 (22.8%) had herpangina. Bronchitis and gastroenteritis were detected in 1 case each. Fever (temperature, >38°C) was noted in 33 (94.3%) cases. The median duration of fever was 2 days (range: 1-3 days). Diarrhea was observed in 7 (20.0%) children, but watery and frequent diarrhea was not common. The age distribution and clinical diagnoses associated with SAFV2 infections were similar to those observed with coxsackievirus B4 infections, which detections showed an epidemic pattern during the study period. CONCLUSION: SAFV2 is a cause of upper respiratory tract illness that exhibits a pathogenicity similar to that of coxsackievirus B.


Assuntos
Infecções por Cardiovirus/epidemiologia , Infecções por Cardiovirus/patologia , Cardiovirus/isolamento & purificação , Infecções Respiratórias/epidemiologia , Infecções Respiratórias/patologia , Adolescente , Distribuição por Idade , Cardiovirus/classificação , Cardiovirus/genética , Infecções por Cardiovirus/virologia , Criança , Pré-Escolar , Infecções por Coxsackievirus/epidemiologia , Infecções por Coxsackievirus/patologia , Infecções por Coxsackievirus/virologia , Feminino , Genótipo , Humanos , Lactente , Masculino , Nasofaringe/virologia , RNA Viral/genética , Infecções Respiratórias/virologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Cultura de Vírus
17.
J Gen Virol ; 91(Pt 11): 2846-56, 2010 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-20660148

RESUMO

The use of metagenomics for virus discovery in clinical samples has opened new opportunities for understanding the aetiology of unexplained illness. This study explores the potential of this sequence-independent approach in a public-health setting, by systematic analysis of samples cultured from patients with unexplained illness through a combination of PCR-based assays and viral metagenomics. In total, 1834 cell-culture isolates were collected between 1994 and 2007 through the Enterovirus Surveillance programme in the Netherlands. During the 13 year period, seven samples that exhibited reproducible cytopathogenic effects in cell culture tested negative in standard PCR assays for a range of viruses. In order to fill the diagnostic gap, viral metagenomics was applied to these culture supernatants, resulting in the rapid identification of viruses in all of the samples. The unexplained samples contained BK polyomavirus, herpes simplex virus, Newcastle disease virus and the recently discovered Saffold viruses (SAFV) (which dominated the unexplained samples; n=4). The full genomic sequences of four SAFV genotype 3 (SAFV-3) viruses, which share 88-93 % nucleotide identity with known SAFV-3 viruses, are reported. Further screening for SAFV in additional cultured, unidentified clinical isolates from 2008 and 2009 resulted in identification of another SAFV-positive sample. Although the pathogenicity of the identified viruses has not been established, this study demonstrates that viral metagenomics is a powerful tool that can be integrated into public-health monitoring efforts to investigate unidentified viruses in cell cultures from clinical isolates where standard PCR assays fail to detect viruses.


Assuntos
Secreções Corporais/virologia , Cardiovirus/isolamento & purificação , Metagenômica/métodos , Virologia/métodos , Viroses/diagnóstico , Vírus BK/genética , Vírus BK/isolamento & purificação , Cardiovirus/classificação , Cardiovirus/genética , Análise por Conglomerados , Enterovirus/genética , Enterovirus/isolamento & purificação , Humanos , Dados de Sequência Molecular , Países Baixos , Vírus da Doença de Newcastle/genética , Vírus da Doença de Newcastle/isolamento & purificação , Filogenia , Reação em Cadeia da Polimerase/métodos , Análise de Sequência de DNA , Homologia de Sequência , Cultura de Vírus/métodos
18.
Emerg Infect Dis ; 16(7): 1158-61, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-20587195

RESUMO

To clarify the potential for respiratory transmission of Saffold cardiovirus (SAFV) and characterize the pathogen, we analyzed respiratory specimens from 1,558 pediatric patients in Beijing. We detected SAFV in 7 (0.5%) patients and identified lineages 1-3. However, because 3 patients had co-infections, we could not definitively say SAFV caused disease.


Assuntos
Cardiovirus/classificação , Infecções Respiratórias/virologia , Adolescente , Cardiovirus/genética , Criança , Pré-Escolar , China , Feminino , Humanos , Lactente , Masculino , Filogenia
20.
J Gen Virol ; 91(Pt 6): 1418-27, 2010 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-20130131

RESUMO

This study identified the complete genomic sequence of four type 2 and type 3 human Saffold-like cardioviruses (SLCVs) isolated in Germany and Brazil. The secondary structures of the SLCV internal ribosome entry sites (IRESs) were deduced based on RNA base-pairing conservation and co-variation, using an established Theiler's murine encephalomyelitis virus (TMEV) IRES structure as a reference. The SLCV IRES was highly similar to that of TMEV, but motifs critical in TMEV for binding of the polypyrimidine tract-binding protein (PTB) were disrupted. In TMEV, corresponding alterations have been associated with reduced neurovirulence in mice. In the non-structural genome region, there was evidence of multiple intertypic recombination events between different SLCV types. Between viruses of the same type, recombination also occurred in the capsid-encoding genome region. There were apparently no recombination events between mouse TMEV and human SLCV. In another genus of the family Picornaviridae, Enterovirus, natural recombination occurs strictly within species and can serve as an additional criterion for delimiting species. Accordingly, the results of this study suggest that SLCV and TMEV may represent distinct species within the genus Cardiovirus.


Assuntos
Cardiovirus/genética , Evolução Molecular , Genoma Viral , RNA Viral/genética , Análise de Sequência de DNA , Animais , Brasil , Cardiovirus/classificação , Cardiovirus/isolamento & purificação , Infecções por Cardiovirus/virologia , Alemanha , Humanos , Camundongos , Dados de Sequência Molecular , Conformação de Ácido Nucleico , Recombinação Genética , Theilovirus/genética , Proteínas não Estruturais Virais/genética , Proteínas Estruturais Virais/genética
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