Activity of membrane, cytosol and lysosome enzymes in organs and blood serum during declamping shock.

The aim of the study was to evaluate the enzyme activity of cellular membranes (GGT), cytosol (ALT, AST) and lysosome (AP, AcP) in the cytosol, whole homogenate and blood serum during declamping shock, following release of abdominal aorta cross-clamping. The aorta was clamped for 60 minutes. An increase in GGT, AP and AcP activities in the cytosol and whole homogenate of the renal cortex, renal medulla, liver, lung, heart and the skeletal muscle occurs after declamping. Rise in the enzymatic activity, especially of acid phosphatase is higher when the aorta above renal arteries was clamped. However, its activity in the blood serum remains unchanged. Alterations in the distribution and the activity of the studied enzymes may indicate that aortic clamping damages the endoplasmic reticulum and lysosomal membranes. Yet, cellular membranes preserve their structural and functional integrity.

Some serum enzymes in burns and their probable correlation with surgical shock--a prospective combined surgicobiochemical study.

Serum enzymes such as phosphohexoisomerase, aldolase and amylase were estimated in serum of 100 patients with thermal burn of different degrees. These enzymes were estimated at the time of admission, at 12th hour, 24th hour, 36th hour, 72nd hour, 7th day and 14th day. The patients with high serum levels of these enzymes till 72nd hour showed grave prognosis and serial measurements of these enzymes might help in predicting the outcome.

[Serum cholinesterase in anesthesia and surgical shock]. / Colinesteraza serica în anestezie si soc chirurgical

The evaluation of the serum cholinesterase activity in various experimental conditions (anesthesized dogs, operated on or not, in shock intoxicated with carbon tetrachloride) indicated various degrees of inhibition of this enzyme, peaking in imbalances that developed during shock or in the carbon tetrachloride intoxication of the animals.