RESUMO
Chrysanthemum pollen extracts analyzed by means of crossed radioimmunoelectrophoresis (CRIE) and RAST-inhibition. Thirty-eight antigens were identified and out of them 10 were able to bind IgE antibodies from sera of patients with pollinosis elicited by these pollens. An allergenic relationship of Chrysanthemum pollen to mugwort pollen was detected in the RAST-inhibition experiments.
Assuntos
Alérgenos/análise , Chrysanthemum cinerariifolium/análise , Plantas/análise , Pólen/imunologia , Alérgenos/imunologia , Animais , Humanos , Hipersensibilidade/etiologia , Hipersensibilidade/imunologia , Imunoeletroforese Bidimensional , Imunoglobulina E/imunologia , Coelhos , Teste de RadioalergoadsorçãoAssuntos
Dermatite Atópica/induzido quimicamente , Dermatite de Contato/etiologia , Perfumes/efeitos adversos , Transtornos de Fotossensibilidade/induzido quimicamente , Chrysanthemum cinerariifolium/análise , Dermatite Atópica/diagnóstico , Dermatite de Contato/diagnóstico , Humanos , Líquens/análise , Transtornos de Fotossensibilidade/diagnóstico , Extratos Vegetais/toxicidadeRESUMO
Of sixty-nine examples of the photosensitivity dermatitis and actinic reticuloid syndrome, nine were found to be allergically sensitive to chrysanthemum oleoresin extract. Although this extract showed evidence in vitro of a phototoxic action we were unable with in vivo studies to explain the connection between the contact allergic sensitivity and the photosensitivity. The fractions responsible for the in vitro phototoxicity appear to be different from those involved in the in vivo contact allergic sensitivity. This study provides further support for the view that in the photosensitivity dermatitis and actinic reticuloid syndrome contact allergic sensitivity is of importance.
Assuntos
Chrysanthemum cinerariifolium , Dermatite de Contato/etiologia , Transtornos de Fotossensibilidade/etiologia , Idoso , Candida albicans/efeitos dos fármacos , Candida albicans/efeitos da radiação , Chrysanthemum cinerariifolium/análise , Dermatite de Contato/diagnóstico , Feminino , Hemólise/efeitos dos fármacos , Hemólise/efeitos da radiação , Humanos , Técnicas In Vitro , Masculino , Pessoa de Meia-Idade , Testes do Emplastro , Transtornos de Fotossensibilidade/diagnóstico , Resinas Vegetais/análise , Resinas Vegetais/farmacologia , Raios Ultravioleta/efeitos adversosRESUMO
A case of occupational chrysanthemum contact dermatitis is reported. Patch tests showed the patient to be sensitized to Chrysanthemum morifolium (Chr M) leaves, flowers, and stems (alcoholic extracts) and to alantolactone. An attempt at desensitization appears to have been successful. Gas chromatography indicated the presence of alantolactone in all the various parts of Chr M, mostly in the flowers. The "maximization test" succeeded in sensitizing guinea pigs to alantolactone.
Assuntos
Chrysanthemum cinerariifolium , Dermatite de Contato/etiologia , Dermatite Ocupacional/etiologia , Lactonas/efeitos adversos , Adulto , Animais , Cromatografia Gasosa , Chrysanthemum cinerariifolium/análise , Dermatite de Contato/diagnóstico , Dessensibilização Imunológica , Cobaias , Humanos , Hipersensibilidade/terapia , Masculino , Testes do EmplastroRESUMO
A method was developed to determine the amount of butylated hydroxy toluene in resmethrin and resmethrin formulations by high-pressure liquid chromatography.
Assuntos
Hidroxitolueno Butilado/análise , Cromatografia Líquida de Alta Pressão , Chrysanthemum cinerariifolium/análise , Cresóis/análise , Piretrinas/análise , Fenômenos Químicos , QuímicaRESUMO
Two rapid and precise methods have been developed for the determination of resmethrin in nonaqueous aerosol formulations. The propellents are evaporated, the residue is dissolved in carbon disulfide, and the resulting solution is analyzed by gas-liquid chromatography or infrared spectroscopy. The accuracies of the methods were confirmed by a collaborative study.
Assuntos
Chrysanthemum cinerariifolium/análise , Piretrinas/análise , Aerossóis , Cromatografia Gasosa , Cromatografia Líquida , Espectrofotometria Infravermelho , Fatores de TempoRESUMO
A gas-liquid chromatographic (GLC) method was developed for the determination of residues of resmethrin ((5-benzyl-3-furyl)methyl cis-trans-(+/-)-2,2-dimethyl-3-(2-methylpropenyl)-cyclopropanecarboxylate) in corn, cornmeal, flour, and wheat. The commodity, fortified with resmethrin, was extracted by tumbling with pentane and transferred to acetonitrile, the fat was partitioned off, and the sample was chromatographed with 3% ethyl acetate in pentane on Florisil containing 0.5% water. The resmethrin residue was determined by GLC with a flame ionization detector. The results were compared with known standards that had undergone the same cleanup procedures. The method was sensitive to concentrations of resmethrin to 0.2 ppm, recoveries averaged 83%, and reproducibility was good.
