RESUMO
OBJECTIVE: To study the secondary metabolites of mangrove endophytic fungus BL321. METHODS: The compounds were isolated by chromatographic technique. The structures were identified by comprehensive physico-chemical properties and spectral methods. RESULTS: Five compounds were isolated and identified as 3,4a-dimethyl-2-oxo-2,4,4a,5,6,7-hexahydronaphtho[2,3-b]furan-5-carboxylic acid(1), cytochalasin C(2), cytochalasin D(3), 19,20-epoxycytochalasin C(4), ergosterol(5). CONCLUSION: Compound 1 is isolated from nature for the first time. Further more, several kinds of strong bioactive compounds were islolate from this fungus indicate that it may develop to be medical source microorganism.
Assuntos
Citocalasinas/isolamento & purificação , Ergosterol/isolamento & purificação , Fungos/química , Furanos/isolamento & purificação , Naftalenos/isolamento & purificação , Rhizophoraceae/microbiologia , China , Citocalasina D/química , Citocalasina D/isolamento & purificação , Citocalasinas/química , Ergosterol/química , Fungos/metabolismo , Furanos/química , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Naftalenos/químicaRESUMO
Chemical investigation of the wood-decayed fungus Xylaria sp. BCC 9653 has led to the isolation of a new methyl aminobenzoate (1) together with eleven known compounds. The structures were established by analysis of spectroscopic data. Cytochalasin D (2), one of the known metabolites, exhibited potent cytotoxicity against African green monkey kidney fibroblast (Vero) cells with an IC(50) value of 0.19 microM.
Assuntos
Antivirais/farmacologia , Citocalasina D/farmacologia , Fibroblastos/efeitos dos fármacos , Rim/efeitos dos fármacos , Madeira , Xylariales/química , para-Aminobenzoatos , Ácido 4-Aminobenzoico/química , Ácido 4-Aminobenzoico/isolamento & purificação , Ácido 4-Aminobenzoico/farmacologia , Animais , Antivirais/química , Antivirais/isolamento & purificação , Células Cultivadas , Chlorocebus aethiops , Citocalasina D/química , Citocalasina D/isolamento & purificação , Fibroblastos/citologia , Concentração Inibidora 50 , Rim/citologia , Rim/metabolismo , Estrutura Molecular , Análise Espectral , Células Vero/efeitos dos fármacosRESUMO
Cytochalasin D which shows marked cytotoxic effects on multi-tumor cells was newly isolated at high content(5.28 mg/g, dry weight) from Fungus Hypocrella bambusae(B.et Br.) Sacc. Its structure was elucidated by spectroscopic methods. Two-dimensional NMR techniques were applied to make complete assignment for the 1H- and 13C-NMR chemical shifts of this compound.
