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1.
Elife ; 82019 08 21.
Artigo em Inglês | MEDLINE | ID: mdl-31433299

RESUMO

Constantly surrounded by kin or alien organisms in nature, eukaryotes and prokaryotes developed various communication systems to coordinate adaptive multi-entity behavior. In complex and overcrowded environments, they require to discriminate relevant signals in a myriad of pheromones to execute appropriate responses. In the human gut commensal Streptococcus salivarius, the cytoplasmic Rgg/RNPP regulator ComR couples competence to bacteriocin-mediated predation. Here, we describe a paralogous sensor duo, ScuR and SarF, which circumvents ComR in order to disconnect these two physiological processes. We highlighted the recurring role of Rgg/RNPP in the production of antimicrobials and designed a robust genetic screen to unveil potent/optimized peptide pheromones. Further mutational and biochemical analyses dissected the modifiable selectivity toward their pheromone and operating sequences at the subtle molecular level. Additionally, our results highlight how we might mobilize antimicrobial molecules while silencing competence in endogenous populations of human microflora and temper gut disorders provoked by bacterial pathogens.


Assuntos
Proteínas de Bactérias/metabolismo , Bacteriocinas/metabolismo , Competência de Transformação por DNA/efeitos dos fármacos , Microbioma Gastrointestinal , Microbiota , Feromônios/metabolismo , Streptococcus salivarius/metabolismo , Antibacterianos/metabolismo , Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos , Redes Reguladoras de Genes/efeitos dos fármacos , Humanos , Streptococcus salivarius/efeitos dos fármacos , Streptococcus salivarius/genética , Streptococcus salivarius/crescimento & desenvolvimento
2.
Curr Microbiol ; 76(8): 950-953, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29332139

RESUMO

Our previous data show that serum albumin can trigger natural transformation in Acinetobacter baumannii. However, extracellular matrix/basal membrane components, norepinephrine, and mucin did not have a significant effect on this process. Therefore, the effect of human products appears to be albumin specific, as both BSA and HSA have been shown to increase of natural transformation.


Assuntos
Acinetobacter baumannii/efeitos dos fármacos , Acinetobacter baumannii/genética , Competência de Transformação por DNA/efeitos dos fármacos , Albumina Sérica Humana/metabolismo , Transformação Bacteriana/efeitos dos fármacos , Humanos
3.
Mol Microbiol ; 109(5): 663-675, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-29995987

RESUMO

Natural genetic transformation is a widespread mechanism of horizontal gene transfer. It involves the internalization of exogenous DNA as single strands and chromosomal integration via homologous recombination, promoting acquisition of new genetic traits. Transformation occurs during a distinct physiological state called competence. In Streptococcus pneumoniae, competence is controlled by ComDE, a two-component system induced by an exported peptide pheromone. DprA is universal among transformable species, strongly induced during pneumococcal competence, and crucial for pneumococcal transformation. Pneumococcal DprA plays three crucial roles in transformation and competence. Firstly, DprA protects internalized DNA from degradation. Secondly, DprA loads the homologous recombinase RecA onto transforming DNA to promote transformation. Finally, DprA interacts with the response regulator ComE to shut-off competence. Here, we explored the effect of altering the cellular levels of DprA on these three roles. High cellular levels of DprA were not required for the primary role of DprA as a transformation-dedicated recombinase loader or for protection of transforming DNA. In contrast, full expression of dprA was required for optimal competence shut-off and transformant fitness. High cellular levels of DprA thus ensure the fitness of pneumococcal transformants by mediating competence shut-off. This promotes survival and propagation of transformants, maximizing pneumococcal adaptive potential.


Assuntos
Proteínas de Bactérias/metabolismo , Competência de Transformação por DNA/fisiologia , Proteínas de Membrana/metabolismo , Streptococcus pneumoniae/fisiologia , Streptococcus pneumoniae/patogenicidade , Transformação Bacteriana/fisiologia , Adaptação Fisiológica , Proteínas de Bactérias/genética , Primers do DNA/genética , Primers do DNA/metabolismo , Competência de Transformação por DNA/efeitos dos fármacos , DNA de Cadeia Simples/genética , DNA de Cadeia Simples/metabolismo , Recombinação Homóloga , Humanos , Isopropiltiogalactosídeo/farmacologia , Proteínas de Membrana/genética , Recombinases Rec A/genética , Recombinases Rec A/metabolismo , Streptococcus pneumoniae/efeitos dos fármacos , Streptococcus pneumoniae/genética , Transformação Bacteriana/efeitos dos fármacos
4.
Mol Oral Microbiol ; 33(5): 378-387, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-29968346

RESUMO

Streptococcus pneumoniae transformation occurs within a short competence window, during which the alternative sigma factor X (SigX) is activated to orchestrate the expression of genes allowing extracellular DNA uptake and recombination. Importantly, antibiotic stress promotes transcriptional changes that may affect more than 20% of the S. pneumoniae genome, including competence genes. These can be activated or repressed, depending on the antibiotic agent. For most antibiotics, however, it remains unknown whether transcriptional effects on competence translate into altered transformability. Here we investigate the effect of antibiotic subinhibitory concentrations on sigX expression using a luciferase reporter, and correlate for the first time with transformation kinetics. Induction of sigX expression by ciprofloxacin and novobiocin correlated with increased and prolonged transformability in S. pneumoniae. The prolonged effect of ciprofloxacin on competence and transformation was also observed in the streptococcal relatives Streptococcus mitis and Streptococcus mutans. In contrast, tetracycline and erythromycin, which induced S. pneumoniae sigX expression, had either an inhibitory or a nonsignificant effect on transformation, whereas streptomycin and the ß-lactam ampicillin, inhibited both sigX expression and transformation. Thus, the results show that antibiotics may vary in their effects on competence, ranging from inhibitory to stimulatory effects, and that responses affecting transcription of sigX do not always correlate with the transformation outcomes. Antibiotics that increase or decrease transformation are of particular clinical relevance, as they may alter the ability of S. pneumoniae to escape vaccines and antibiotics.


Assuntos
Antibacterianos/farmacologia , Proteínas de Bactérias/genética , Competência de Transformação por DNA/efeitos dos fármacos , Streptococcus/efeitos dos fármacos , Streptococcus/genética , Regulação Bacteriana da Expressão Gênica , Fator sigma
5.
Sci Rep ; 6: 35738, 2016 10 20.
Artigo em Inglês | MEDLINE | ID: mdl-27760994

RESUMO

Efficiency of yeast transformation is determined by the rate of yeast endocytosis. The aim of this study was to investigate the effect of introducing amino acids and other nutrients (inositol, adenine, or p-aminobenzoic acid) in the transformation medium to develop a highly efficient yeast transformation protocol. The target of rapamycin complex 1 (TORC1) kinase signalling complex influences the rate of yeast endocytosis. TORC signaling is induced by amino acids in the media. Here, we found that increasing the concentration of amino acids and other nutrients in the growth media lead to an increase yeast transformation efficiency up to 107 CFU per µg plasmid DNA and per 108 cells with a 13.8 kb plasmid DNA. This is over 130 times that of current published methods. This improvement may facilitate more efficient experimentation in which transformation efficiency is critical, such as yeast two-hybrid screening.


Assuntos
Meios de Cultura/química , Competência de Transformação por DNA/efeitos dos fármacos , Saccharomyces cerevisiae/efeitos dos fármacos , Saccharomyces cerevisiae/genética , Fatores Biológicos/metabolismo
6.
Antimicrob Agents Chemother ; 60(8): 4920-9, 2016 08.
Artigo em Inglês | MEDLINE | ID: mdl-27270286

RESUMO

The increasing frequency of bacteria showing antimicrobial resistance (AMR) raises the menace of entering into a postantibiotic era. Horizontal gene transfer (HGT) is one of the prime reasons for AMR acquisition. Acinetobacter baumannii is a nosocomial pathogen with outstanding abilities to survive in the hospital environment and to acquire resistance determinants. Its capacity to incorporate exogenous DNA is a major source of AMR genes; however, few studies have addressed this subject. The transformation machinery as well as the factors that induce natural competence in A. baumannii are unknown. In this study, we demonstrate that naturally competent strain A118 increases its natural transformation frequency upon the addition of Ca(2+)or albumin. We show that comEA and pilQ are involved in this process since their expression levels are increased upon the addition of these compounds. An unspecific protein, like casein, does not reproduce this effect, showing that albumin's effect is specific. Our work describes the first specific inducers of natural competence in A. baumannii Overall, our results suggest that the main protein in blood enhances HGT in A. baumannii, contributing to the increase of AMR in this threatening human pathogen.


Assuntos
Infecções por Acinetobacter/microbiologia , Acinetobacter baumannii/efeitos dos fármacos , Acinetobacter baumannii/genética , Cálcio/farmacologia , Infecção Hospitalar/microbiologia , Competência de Transformação por DNA/efeitos dos fármacos , Albumina Sérica/farmacologia , DNA/genética , Competência de Transformação por DNA/genética , Farmacorresistência Bacteriana/genética , Transferência Genética Horizontal/efeitos dos fármacos , Transferência Genética Horizontal/genética , Genes Bacterianos/genética , Humanos
8.
Sci Rep ; 5: 16033, 2015 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-26526572

RESUMO

Natural transformation is the process by which bacteria can actively take up and integrate exogenous DNA thereby providing a source of genetic diversity. Under specific growth conditions the coordinated expression of several genes--a situation referred to as "competence"--allows bacteria to assemble a highly processive and dedicated system that can import high molecular weight DNA. Within the cell these large imported DNA molecules are protected from degradation and brought to the chromosome for recombination. Here, we report elevated expression of mreB during competence in the Gram-negative pathogen Legionella pneumophila. Interestingly a similar observation had previously been reported in the distantly-related Gram-positive organism Bacillus subtilis. MreB is often viewed as the bacterial actin homolog contributing to bacterial morphogenesis by coordinating peptidoglycan-synthesising complexes. In addition MreB is increasingly found to be involved in a growing number of processes including chromosome segregation and motor-driven motility. Using genetic and pharmacological approaches, we examined the possible role of MreB during natural transformation in L. pneumophila. Our data show that natural transformation does not require MreB dynamics and exclude a direct role of MreB filaments in the transport of foreign DNA and its recombination in the chromosome.


Assuntos
Actinas/metabolismo , Proteínas de Bactérias/metabolismo , Citoesqueleto/metabolismo , Legionella pneumophila/metabolismo , Actinas/antagonistas & inibidores , Actinas/genética , Proteínas de Bactérias/antagonistas & inibidores , Proteínas de Bactérias/genética , Segregação de Cromossomos , Citoesqueleto/efeitos dos fármacos , Competência de Transformação por DNA/efeitos dos fármacos , Farmacorresistência Bacteriana/efeitos dos fármacos , Genes Bacterianos , Morfogênese/fisiologia , Plasmídeos/genética , Plasmídeos/metabolismo , Tioureia/análogos & derivados , Tioureia/farmacologia , Regulação para Cima
9.
Appl Environ Microbiol ; 81(16): 5622-31, 2015 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-26070670

RESUMO

Genetic competence in Streptococcus mutans is a transient state that is regulated in response to multiple environmental inputs. These include extracellular pH and the concentrations of two secreted peptides, designated CSP (competence-stimulating peptide) and XIP (comX-inducing peptide). The role of environmental cues in regulating competence can be difficult to disentangle from the effects of the organism's physiological state and its chemical modification of its environment. We used microfluidics to control the extracellular environment and study the activation of the key competence gene comX. We find that the comX promoter (PcomX) responds to XIP or CSP only when the extracellular pH lies within a narrow window, about 1 pH unit wide, near pH 7. Within this pH range, CSP elicits a strong PcomX response from a subpopulation of cells, whereas outside this range the proportion of cells expressing comX declines sharply. Likewise, PcomX is most sensitive to XIP only within a narrow pH window. While previous work suggested that comX may become refractory to CSP or XIP stimulus as cells exit early exponential phase, our microfluidic data show that extracellular pH dominates in determining sensitivity to XIP and CSP. The data are most consistent with an effect of pH on the ComR/ComS system, which has direct control over transcription of comX in S. mutans.


Assuntos
Proteínas de Bactérias/biossíntese , Competência de Transformação por DNA/efeitos dos fármacos , Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos , Streptococcus mutans/efeitos dos fármacos , Streptococcus mutans/genética , Fatores de Transcrição/biossíntese , Técnicas Bacteriológicas , Perfilação da Expressão Gênica , Concentração de Íons de Hidrogênio , Microfluídica , Regiões Promotoras Genéticas , Ligação Proteica
10.
J Bacteriol ; 196(2): 337-44, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24187089

RESUMO

Helicobacter pylori uses natural competence and homologous recombination to adapt to the dynamic environment of the stomach mucosa and maintain chronic colonization. Although H. pylori competence is constitutive, its rate of transformation is variable, and little is known about factors that influence it. To examine this, we first determined the transformation efficiency of H. pylori strains under low O2 (5% O2, 7.6% CO2, 7.6% H2) and high O2 (15% O2, 2.9% CO2, 2.9% H2) conditions using DNA containing an antibiotic resistance marker. H. pylori transformation efficiency was 6- to 32-fold greater under high O2 tension, which was robust across different H. pylori strains, genetic loci, and bacterial growth phases. Since changing the O2 concentration for these initial experiments also changed the concentrations of CO2 and H2, transformations were repeated under conditions where O2, CO2, and H2 were each varied individually. The results showed that the increase in transformation efficiency under high O2 was largely due to a decrease in CO2. An increase in pH similar to that caused by low CO2 was also sufficient to increase transformation efficiency. These results have implications for the physiology of H. pylori in the gastric environment, and they provide optimized conditions for the laboratory construction of H. pylori mutants using natural transformation.


Assuntos
Competência de Transformação por DNA/efeitos dos fármacos , Helicobacter pylori/efeitos dos fármacos , Helicobacter pylori/fisiologia , Transformação Bacteriana , Dióxido de Carbono/metabolismo , Helicobacter pylori/genética , Hidrogênio/metabolismo , Oxigênio/metabolismo
11.
J Bacteriol ; 195(18): 4085-93, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-23836866

RESUMO

Natural competence is a process by which bacteria construct a membrane-associated machine for the uptake and integration of exogenous DNA. Many bacteria harbor genes for the DNA uptake machinery and yet are recalcitrant to DNA uptake for unknown reasons. For example, domesticated laboratory strains of Bacillus subtilis are renowned for high-frequency natural transformation, but the ancestral B. subtilis strain NCIB3610 is poorly competent. Here we find that endogenous plasmid pBS32 encodes a small protein, ComI, that inhibits transformation in the 3610 strain. ComI is a single-pass trans-membrane protein that appears to functionally inhibit the competence DNA uptake machinery. Functional inhibition of transformation may be common, and abolishing such inhibitors could be the key to permitting convenient genetic manipulation of a variety of industrially and medically relevant bacteria.


Assuntos
Bacillus subtilis/genética , Proteínas de Bactérias/farmacologia , Competência de Transformação por DNA/efeitos dos fármacos , Proteínas de Membrana/farmacologia , Plasmídeos/genética , Transformação Bacteriana , Bacillus subtilis/crescimento & desenvolvimento , Bacillus subtilis/metabolismo , Bacillus subtilis/fisiologia , Proteínas de Bactérias/genética , Biofilmes/crescimento & desenvolvimento , DNA Bacteriano/genética , Regulação Bacteriana da Expressão Gênica , Proteínas de Membrana/genética , Reação em Cadeia da Polimerase , Transformação Bacteriana/efeitos dos fármacos
12.
Curr Opin Microbiol ; 15(5): 570-6, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22910199

RESUMO

Bacterial transformation is a programmed process resulting in genetic transfer and diversity. It relies on the development of competence via regulatory circuits which are diverse and tailored to the particular lifestyle of each species. Despite this diversity, some species have been reported to trigger competence in response to antibiotics. Here, we review these recent findings, which reinforce the view that competence is a stress response and can substitute for SOS in bacteria lacking it.


Assuntos
Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Bactérias/genética , Competência de Transformação por DNA/efeitos dos fármacos , Transformação Genética/efeitos dos fármacos , Evolução Molecular , Resposta SOS em Genética , Estresse Fisiológico
13.
PLoS Pathog ; 7(9): e1002241, 2011 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-21909280

RESUMO

Competence stimulating peptide (CSP) is a 17-amino acid peptide pheromone secreted by Streptococcus pneumoniae. Upon binding of CSP to its membrane-associated receptor kinase ComD, a cascade of signaling events is initiated, leading to activation of the competence regulon by the response regulator ComE. Genes encoding proteins that are involved in DNA uptake and transformation, as well as virulence, are upregulated. Previous studies have shown that disruption of key components in the competence regulon inhibits DNA transformation and attenuates virulence. Thus, synthetic analogues that competitively inhibit CSPs may serve as attractive drugs to control pneumococcal infection and to reduce horizontal gene transfer during infection. We performed amino acid substitutions on conserved amino acid residues of CSP1 in an effort to disable DNA transformation and to attenuate the virulence of S. pneumoniae. One of the mutated peptides, CSP1-E1A, inhibited development of competence in DNA transformation by outcompeting CSP1 in time and concentration-dependent manners. CSP1-E1A reduced the expression of pneumococcal virulence factors choline binding protein D (CbpD) and autolysin A (LytA) in vitro, and significantly reduced mouse mortality after lung infection. Furthermore, CSP1-E1A attenuated the acquisition of an antibiotic resistance gene and a capsule gene in vivo. Finally, we demonstrated that the strategy of using a peptide inhibitor is applicable to other CSP subtype, including CSP2. CSP1-E1A and CSP2-E1A were able to cross inhibit the induction of competence and DNA transformation in pneumococcal strains with incompatible ComD subtypes. These results demonstrate the applicability of generating competitive analogues of CSPs as drugs to control horizontal transfer of antibiotic resistance and virulence genes, and to attenuate virulence during infection by S. pneumoniae.


Assuntos
Proteínas de Bactérias/fisiologia , Competência de Transformação por DNA/efeitos dos fármacos , Transferência Genética Horizontal/efeitos dos fármacos , Streptococcus pneumoniae/genética , Virulência/efeitos dos fármacos , Amidoidrolases/antagonistas & inibidores , Substituição de Aminoácidos , Animais , Proteínas de Bactérias/antagonistas & inibidores , Proteínas de Bactérias/genética , Farmacorresistência Bacteriana/efeitos dos fármacos , Camundongos , Infecções Pneumocócicas/prevenção & controle , Regulon/efeitos dos fármacos , Streptococcus pneumoniae/fisiologia
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