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1.
Diagn Microbiol Infect Dis ; 94(2): 160-164, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30642722

RESUMO

The correlation between the Food and Drug Administration-cleared C6 enzyme immunoassay (EIA) C6 index values and a diagnosis of Lyme disease has not been examined. We used pooled patient-level data from 5 studies of adults and children with Lyme disease and control subjects who were tested with the C6 EIA. We constructed a receiver operating characteristic curve using regression clustered by study and measured the area under the curve (AUC) to examine the accuracy of the C6 index values in differentiating between patients with noncutaneous Lyme disease and control subjects. In the 4821 included patients, the C6 index value had excellent ability to distinguish between patients with noncutaneous Lyme disease and control subjects [AUC 0.99; 95% confidence interval (CI) 0.99-1.00]. An index value cut point of ≥3.0 had a sensitivity of 90.9% (95% CI, 87.8-93.3) and specificity of 99.0% (95% CI, 98.6-99.2%) for Lyme disease.


Assuntos
Complemento C6/análise , Técnicas Imunoenzimáticas/métodos , Doença de Lyme/diagnóstico , Testes Sorológicos/métodos , Humanos , Doença de Lyme/patologia , Curva ROC , Sensibilidade e Especificidade
2.
Arthritis Res Ther ; 13(6): R181, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-22044644

RESUMO

INTRODUCTION: Although systemic autoimmune diseases (SAID) share many clinical and laboratory features, whether they also share some common features of pathogenesis remains unclear. We assessed plasma proteomic profiles among different SAID for evidence of common molecular pathways that could provide insights into pathogenic mechanisms shared by these diseases. METHODS: Differential quantitative proteomic analyses (one-dimensional reverse-phase liquid chromatography-mass spectrometry) were performed to assess patterns of plasma protein expression. Monozygotic twins (four pairs discordant for systemic lupus erythematosus, four pairs discordant for juvenile idiopathic arthritis and two pairs discordant for juvenile dermatomyositis) were studied to minimize polymorphic gene effects. Comparisons were also made to 10 unrelated, matched controls. RESULTS: Multiple plasma proteins, including acute phase reactants, structural proteins, immune response proteins, coagulation and transcriptional factors, were differentially expressed similarly among the different SAID studied. Multivariate Random Forest modeling identified seven proteins whose combined altered expression levels effectively segregated affected vs. unaffected twins. Among these seven proteins, four were also identified in univariate analyses of proteomic data (syntaxin 17, α-glucosidase, paraoxonase 1, and the sixth component of complement). Molecular pathway modeling indicated that these factors may be integrated through interactions with a candidate plasma biomarker, PON1 and the pro-inflammatory cytokine IL-6. CONCLUSIONS: Together, these data suggest that different SAID may share common alterations of plasma protein expression and molecular pathways. An understanding of the mechanisms leading to the altered plasma proteomes common among these SAID may provide useful insights into their pathogeneses.


Assuntos
Artrite Juvenil/metabolismo , Dermatomiosite/metabolismo , Lúpus Eritematoso Sistêmico/metabolismo , Proteoma/análise , Proteômica/métodos , Gêmeos Monozigóticos , Adulto , Artrite Juvenil/sangue , Artrite Juvenil/genética , Arildialquilfosfatase/sangue , Criança , Cromatografia Líquida , Complemento C6/análise , Dermatomiosite/sangue , Dermatomiosite/genética , Feminino , Humanos , Immunoblotting , Interleucina-6/sangue , Lúpus Eritematoso Sistêmico/sangue , Lúpus Eritematoso Sistêmico/genética , Masculino , Análise Multivariada , Proteínas Qa-SNARE/sangue , Transdução de Sinais/genética , Transdução de Sinais/imunologia , Espectrometria de Massas por Ionização por Electrospray , alfa-Glucosidases/sangue
5.
Eur J Clin Microbiol Infect Dis ; 23(8): 615-8, 2004 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-15243815

RESUMO

It was recently reported that antibody to C(6), a peptide that reproduces an invariable region of the VlsE lipoprotein of Borrelia burgdorferi, declined in titer by a factor of four or more in a significant proportion of patients after successful antibiotic treatment of acute localized or disseminated Lyme borreliosis. The present study evaluated the C(6) test as a predictor of therapy outcome in a population of patients with post-treatment Lyme disease syndrome. The serum specimens tested were from patients with well-documented, previously treated Lyme borreliosis who had persistent musculoskeletal or neurocognitive symptoms. All of the patients had participated in a recent double-blind, placebo-controlled antibiotic trial in which serum samples were collected at baseline and 6 months thereafter, i.show $132#e. 3 months following treatment termination. In this patient population no correlation was found between a decline of C(6) antibody titer of any magnitude and treatment or clinical outcome. Antibodies to C(6) persisted in these patients with post-treatment Lyme disease syndrome following treatment, albeit at a markedly lower prevalence and titer than in untreated patients with acute disseminated Lyme disease. The results indicate that C(6) antibody cannot be used to assess treatment outcome or the presence of active infection in this population.


Assuntos
Antibacterianos/uso terapêutico , Biomarcadores/sangue , Borrelia burgdorferi/isolamento & purificação , Complemento C6/análise , Doença de Lyme/tratamento farmacológico , Doença de Lyme/imunologia , Anticorpos Antibacterianos/análise , Anticorpos Antibacterianos/efeitos dos fármacos , Distribuição de Qui-Quadrado , Método Duplo-Cego , Feminino , Humanos , Masculino , Valor Preditivo dos Testes , Probabilidade , Prognóstico , Recidiva , Valores de Referência , Sensibilidade e Especificidade , Índice de Gravidade de Doença , Resultado do Tratamento
6.
Tunis Med ; 81(3): 195-9, 2003 Mar.
Artigo em Francês | MEDLINE | ID: mdl-12793071

RESUMO

Ten Tunisian patients, with homozygote sickle cell disease and asplenia were studied to investigate and to determine possible immunological function defects. Obtained results directed us to an abnormality of the alternate complement pathway activation which is expressed by a decreased hémolytic activity, while the classic pathway is normal. Quantification of C3, C4, C5, C6, C7 and factor B by immunochemical assay were normal, whereas factor B functional activity was depressed to a mean level of about half of normal in eight patients, IgG was increased in one subject and IgA in two others. Numeration of Band T cells revealed slight decrease in proportion of CD3 and CD4 at one patient associated with an increase in B cells, but normal or increased absolute numbers of all cells population.


Assuntos
Anemia Falciforme/imunologia , Proteínas do Sistema Complemento/imunologia , Adolescente , Anemia Falciforme/genética , Linfócitos B/imunologia , Criança , Pré-Escolar , Complemento C3/análise , Complemento C4/análise , Complemento C5/análise , Complemento C6/análise , Complemento C7/análise , Fator B do Complemento/análise , Proteínas do Sistema Complemento/análise , Técnica Direta de Fluorescência para Anticorpo , Homozigoto , Humanos , Imunodifusão , Imunoglobulina A/análise , Imunoglobulina G/análise , Imunoglobulina M/análise , Linfócitos T/imunologia
7.
J Neuroimmunol ; 123(1-2): 91-101, 2002 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-11880154

RESUMO

The brain is largely protected from damage due to infection, trauma, and aberrant processes by the innate immune system. These studies were undertaken to determine whether neurons in normal brains constitutively express complement components. In situ hybridization and immunohistochemical studies with specific riboprobes and antibodies, respectively, revealed that most hippocampal neurons, many pyramidal cortical neurons and cerebellar Purkinje neurons in normal murine brains constitutively express C3, C5 and C6. The constitutive expression by neuronal subsets of components of the complement activation and membrane attack pathways suggests that the complement system represents a "first line" of host defense in the brain.


Assuntos
Encéfalo/imunologia , Complemento C3/análise , Complemento C5/análise , Complemento C6/análise , Neurônios/imunologia , Animais , Complemento C3/genética , Complemento C3/imunologia , Complemento C5/genética , Complemento C5/imunologia , Complemento C6/genética , Complemento C6/imunologia , Camundongos , Camundongos Endogâmicos C57BL , RNA Mensageiro/análise , Coelhos , Proteínas Recombinantes/imunologia
8.
9.
Scand J Immunol ; 48(3): 261-8, 1998 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-9743210

RESUMO

Sera genetically deficient in either the alpha-gamma or the beta-subunit of complement component C8 virtually lack haemolytic activity. We have studied the formation and the structural organization of the soluble terminal complement complex (TCC) assembled in these sera following activation with cobra venom factor (CVF). The TCC concentration in the activated C8alpha-gamma and C8beta-deficient samples was 0.2% and 4%, respectively, when compared with zymosan-activated normal serum. TCC was purified from the activated C8beta-deficient samples by affinity chromatography and analysed by immunoblotting and enzyme immunoassay. No C8beta was detected in one TCC preparation, while 7% of the normal level was present in the other. The level of the other terminal components, including that of C8alpha-gamma, was normal. The ability of C8alpha-gamma to promote the assembly of TCC in the presence of a limited amount of C8beta or in the apparent absence of this subunit was confirmed using purified components, by mixing C5b6 and either of the purified C8 subunits together with C7 and C9. These data show that soluble TCC can be formed in C8beta-deficient sera that contain little or no C8beta.


Assuntos
Complemento C8/deficiência , Complexo de Ataque à Membrana do Sistema Complemento/química , Complemento C5/análise , Complemento C6/análise , Complemento C7/análise , Complemento C8/análise , Complemento C9/análise , Complexo de Ataque à Membrana do Sistema Complemento/imunologia , Complexo de Ataque à Membrana do Sistema Complemento/isolamento & purificação , Humanos , Técnicas Imunoenzimáticas , Solubilidade , Fatores de Tempo
10.
J Immunol ; 157(6): 2282-90, 1996 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-8805625

RESUMO

The sixth complement component (C6) is a late-acting complement protein that participates in the assembly of the membrane attack complex. C6 and most of the complement proteins are mainly synthesized in the liver. However, the human hepatoma-derived cell line Hep-G2, which produces the majority of complement proteins, synthesizes traces of C6. Here, we have isolated and characterized the human C6 promoter. Approximately 1 kb of C6 upstream sequence is shown to be sufficient to achieve tissue-specific expression of a luciferase reporter gene in two hepatic (Hep-G2 and Hep-3B) and two extrahepatic cell lines (fibroblast M1 and HeLa) in a manner similar to endogenous C6. There are wide differences in C6 mRNA expression among the four cell lines, whereas Hep-3B expresses high levels of C6, Hep-G2 and M1 poorly synthesize C6, and HeLa completely lacks C6 expression. Deletional and mutational analysis demonstrates that a C/EBP (CCAAT/enhancer binding protein) site located at -67 is required for C6 expression in Hep-3B cells, but it has little effect in M1 and Hep-G2 cells. Electrophoretic mobility shift assays show that this sequence binds to a C/EBP alpha using Hep-3B nuclear extract, but a negligible activity is detected using a Hep-G2 extract. To further investigate whether C/EBP alpha is the limiting factor for C6 expression, we have transfected a C/EBP alpha expression vector into Hep-G2 and Ml cells. C/EBP alpha expression vector dramatically trans-activates the luciferase reporter gene controlled by the C6 promoter, and it partially restores C6 mRNA expression in Hep-G2 cells.


Assuntos
Complemento C6/genética , Proteínas de Ligação a DNA/genética , Elementos Facilitadores Genéticos/imunologia , Regiões Promotoras Genéticas/imunologia , Animais , Sequência de Bases , Carcinoma Hepatocelular/genética , Carcinoma Hepatocelular/imunologia , Complemento C6/análise , Proteínas de Ligação a DNA/imunologia , Eletroforese em Gel de Poliacrilamida , Vetores Genéticos/imunologia , Humanos , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/imunologia , Dados de Sequência Molecular , Especificidade de Órgãos/genética , Especificidade de Órgãos/imunologia , Análise de Sequência de DNA , Fatores de Transcrição/genética , Fatores de Transcrição/imunologia , Ativação Transcricional , Transfecção/imunologia , Células Tumorais Cultivadas
11.
Hepatology ; 24(1): 118-22, 1996 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-8707249

RESUMO

Complement receptor type 1 (CR1) is an integral membrane protein of many hematopoietic cells and is found in a soluble form in plasma. Preliminary data have indicated that soluble complement receptor 1 (sCR1) levels in serum were increased in patients with cirrhosis. In this study, sCR1 was measured in patients with various liver diseases with a newly established enzyme-linked immunosorbent assay (ELISA). sCR1 level was elevated in chronic active hepatitis C (24 patients, 62.6 +/- 31 ng/ML; 31 normal controls, 31.4 +/- 7.8 ng/mL, P < .001), and in cirrhosis (35 patients, 143.7 +/- 61 ng/mL, P < .001). The levels increased transiently in 3 patients who had amanita phalloides intoxication. In 25 patients with advanced cirrhosis (pretransplantation screening), there were significant inverse correlations between sCR1 and both the prothrombin index (rs = -.60, P < .002) and the aminopyrine breath test (rs = -.51, P < .01). Following liver transplantation, the levels dropped from 166 +/- 70 to 49 +/- 24 ng/mL (P < .0001), and serial measurements in the posttransplantation period showed a correlation with liver dysfunction, regardless of etiology. Since CR1 is not produced by hepatocytes, the most likely explanation for the increased level of sCR1 is reduced is reduced catabolism. Thus, sCR1 may be added to the short list of large glycoproteins that accumulate in liver disease.


Assuntos
Hepatite C/sangue , Cirrose Hepática/sangue , Hepatopatias/sangue , Transplante de Fígado/fisiologia , Receptores de Complemento/análise , Amanita , Aminopirina/análise , Biomarcadores/sangue , Complemento C6/análise , Complemento C7/análise , Ensaio de Imunoadsorção Enzimática , Seguimentos , Hepatite B/sangue , Hepatite B/complicações , Hepatite B/imunologia , Hepatite C/complicações , Hepatite C/imunologia , Humanos , Cirrose Hepática/etiologia , Cirrose Hepática/imunologia , Hepatopatias/imunologia , Transplante de Fígado/imunologia , Intoxicação Alimentar por Cogumelos/sangue , Intoxicação Alimentar por Cogumelos/imunologia , Valores de Referência
12.
Rheumatol Int ; 13(4): 139-46, 1993.
Artigo em Inglês | MEDLINE | ID: mdl-8310205

RESUMO

We have studied synthesis of the complement components and regulatory proteins of the alternative pathway and the membrane attack complex in synovial membrane. RNA was extracted from synovial tissue of patients with rheumatoid arthritis (RA) or osteoarthritis (OA) as well as from normal synovial membrane. Dot blot analysis showed the presence of mRNAs for all the complement components and regulatory proteins (C3, factor B, factor D, C5, C6, C7, C9, factor H, factor I, S-protein, SP-40, 40, DAF, MCP, CR1, CD59), except for properdin, C8 alpha, C8 beta and C8 gamma in all three types of synovial membrane studied. In an attempt to determine which components were synthesised by each cell type, monocytes (mononuclear phagocytes), human umbilical vein endothelial cells (HUVEC), synovial membrane fibroblasts (from normal, OA and RA synovial membrane) and peripheral blood lymphocytes were cultured in vitro and secretion rates of individual components were measured and total cellular RNA analysed by northern blotting. Monocytes secreted properdin, C3, and factor H but not factor B, factor I, C5, C6, C7, C8 or C9. Fibroblasts and endothelial cells secreted factor B, factor H and factor I, but not properdin, C5, C6, C7, C8 or C9. Lymphocytes did not secrete any of these components. mRNAs encoding C3, factor B, factor H, S-protein, SP-40, 40, MCP and DAF were detected in all three other cell types (monocytes, fibroblasts and HU-VEC), but factor I and CD59 mRNAs were not detected in monocytes. C5, C6, C7, C8 alpha, C8 beta, CD8 gamma and C9 mRNAs were not detected in any of the cell types studied.(ABSTRACT TRUNCATED AT 250 WORDS)


Assuntos
Artrite Reumatoide/metabolismo , Complemento C3/análise , Fator H do Complemento/análise , Complexo de Ataque à Membrana do Sistema Complemento/análise , Via Alternativa do Complemento/fisiologia , Glicoproteínas de Membrana/análise , Osteoartrite/metabolismo , Membrana Sinovial/química , Membrana Sinovial/fisiologia , Artrite Reumatoide/patologia , Artrite Reumatoide/fisiopatologia , Sequência de Bases , Northern Blotting , Células Cultivadas , Complemento C3/genética , Complemento C3/metabolismo , Complemento C5/análise , Complemento C5/genética , Complemento C5/metabolismo , Complemento C6/análise , Complemento C6/genética , Complemento C6/metabolismo , Complemento C7/análise , Complemento C7/genética , Complemento C7/metabolismo , Complemento C9/análise , Complemento C9/genética , Complemento C9/metabolismo , Fator H do Complemento/genética , Fator H do Complemento/metabolismo , Complexo de Ataque à Membrana do Sistema Complemento/metabolismo , Complexo de Ataque à Membrana do Sistema Complemento/fisiologia , Endotélio Vascular/química , Endotélio Vascular/citologia , Endotélio Vascular/fisiologia , Fibroblastos/química , Fibroblastos/citologia , Fibroblastos/fisiologia , Humanos , Leucócitos/química , Leucócitos/patologia , Leucócitos/fisiologia , Glicoproteínas de Membrana/genética , Glicoproteínas de Membrana/metabolismo , Dados de Sequência Molecular , Monócitos/química , Monócitos/patologia , Monócitos/fisiologia , Sondas de Oligonucleotídeos , Osteoartrite/patologia , Osteoartrite/fisiopatologia , RNA Mensageiro/análise , RNA Mensageiro/genética , Membrana Sinovial/patologia , Vitronectina
13.
Immunology ; 75(1): 10-6, 1992 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-1537585

RESUMO

A sensitive ELISA assay was used to quantitate serum complement component C6 concentrations. Levels in the range 0.3-3 micrograms/ml were measured in samples from eight individuals (four separate pedigrees) and two subjects with subtotal combined C6/C7 deficiency who have been reported previously. We defined C6 levels in this range as subtotal C6 deficiency (C6SD). In contrast, C6 deficiency with levels below 0.03 micrograms/ml was defined as C6Q0. C6Q0 has been found in 29 unrelated cases which have already been reported. Investigations of the properties of the C6 found in the C6SD subjects showed it to be haemolytically active and able to incorporate into the terminal complement complex. The protein had a relative molecular weight (Mr) of approximately 86% of normal C6 and this Mr was identical to that of the C6 of one combined deficient subject. The Mr of the C6 of the other combined deficient subject was previously estimated as 79% of the Mr of normal C6. Isoelectric focusing (IEF) analysis with band development by haemolytic overlay revealed that all C6SD samples produced an identical weak C6 band pattern anodal to normal C6A bands. The C7 IEF patterns of the two combined deficient subjects were identical, and the C6 IEF patterns of both were identical to those of the C6SD subjects. Thus the C6 of the combined deficient subjects is probably the same abnormal protein found in the C6SD individuals. None of the C6SD or combined deficient subjects have had meningococcal disease and it may be that low C6 levels afford some protection.


Assuntos
Complemento C6/química , Complemento C6/deficiência , Adulto , Criança , Complemento C6/análise , Complemento C7/análise , Complexo de Ataque à Membrana do Sistema Complemento/análise , Ensaio de Imunoadsorção Enzimática , Feminino , Hemólise/imunologia , Humanos , Immunoblotting , Focalização Isoelétrica , Masculino , Pessoa de Meia-Idade , Peso Molecular , Linhagem
14.
Clin Exp Immunol ; 83(3): 430-7, 1991 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-2004484

RESUMO

Two sensitive sandwich ELISAs based on monoclonal antibodies directed to native C6 and C7 allowed the detection and quantitation of these complement proteins in 20 out of 37 serum samples from individuals who had previously been classified as deficient in these proteins as assessed by immunochemical and/or functional assays. Furthermore, serum from four C6-deficient and one combined C6-/C7-deficient individual showed an increase in the terminal complement complex (TCC) and a decrease in native C6 and C7 after complement activation as assayed by specific ELISAs. Despite their (incomplete) deficiencies, these individuals therefore possess functionally active terminal complement proteins with respect to their ability to generate the TCC. As these individuals have no history of a susceptibility to neisserial infections, even low concentrations of functionally active C6 and C7 may provide sufficient protection against those micro-organisms whose destruction requires TCC formation.


Assuntos
Complemento C6/análise , Complemento C6/deficiência , Complemento C7/análise , Complemento C7/deficiência , Anticorpos Monoclonais , Atividade Bactericida do Sangue/imunologia , Ativação do Complemento , Complemento C9/fisiologia , Ensaio de Atividade Hemolítica de Complemento , Eletroforese em Gel de Poliacrilamida , Ensaio de Imunoadsorção Enzimática , Humanos , Immunoblotting , Manejo de Espécimes , Temperatura , Zimosan
15.
Chem Pharm Bull (Tokyo) ; 39(2): 432-6, 1991 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-2054869

RESUMO

The effects of serine protease inhibitors, diisopropyl fluorophosphate (DFP) and phenylmethanesulfonyl fluoride (PMSF), on hemolytic activity of C6 were reinvestigated. C6 was inactivated in a range of 1-10 mM by both of the inhibitors as previously reported. Limited proteolytic digestion was also studied to elucidate the functional and structural domains of C6. The major fragments produced by trypsin, plasmin, or lysyl endopeptidase could not be separated unless disulfide bonds were disrupted, but Staphylococcus aureus V8 protease yielded several fragments, each of which was not linked by disulfide bond. When C6 labeled with [3H]DFP was subjected to limited digestion with V8 protease, a fragment with a molecular weight of 38 kilodaltons (kDa) was mainly labeled and other fragments of 53 kDa and 26.4 kDa were also faintly labeled, while fragment 35 kDa wasn't labeled, indicating specific domains reactive with DFP. On the other hand, when C6 with or without DFP treatment was digested with V8 protease and those fragments were incubated with C5 and subjected to sucrose density ultracentrifugation, fragments 53, 38, 35 and 27.5 kDa interacted with C5 in both cases. These results suggest that C6 modified by DFP can interact with C5, and the amino-terminal sequences of fragment 38 and 35 kDa suggest the binding domain of C6 with C5 takes place within the two short consensus repeats.


Assuntos
Complemento C6/análise , Humanos , Hidrólise , Serina Endopeptidases
19.
Jikken Dobutsu ; 34(2): 173-82, 1985 Apr.
Artigo em Japonês | MEDLINE | ID: mdl-4018150

RESUMO

A two-way selective experiment for total hemolytic complement activity (CH50) was carried out in a colony of New Zealand White rabbits for the purpose of developing hereditary deficiency of complement component and estimating the realized heritability (h2) of CH50. The results obtained were as follows. 1) The mean value of CH50 with a standard error (SE) in 203 adults rabbits was 9.0 +/- 0.2 U/ml, and the range of CH50 was 2 to 18 U/ml. 2) Individual differences of CH50 in rabbits were comparatively stable regardless of time and season. 3) The realized heritability (h2) of CH50 was estimated to approximately 0.3. 4) Two rabbits with a hereditary C8 alpha-gamma deficiency were obtained from a cross between low CH50 individuals (male: 5.9 U/ml X female: 5.6 U/ml). From other crosses (male: 3.2 U/ml X female: 5.6, 5.7 U/ml), five rabbits with a hereditary C6 deficiency were obtained. 5) The frequencies of C8 alpha-gamma and C6 deficient genes in the colony were estimated to at least 0.005, 0.003, respectively. 6) It was suggested that a downward selection for CH50 was a useful method for developing hereditary deficiency of complement component in the rabbit.


Assuntos
Complemento C6/deficiência , Complemento C8/deficiência , Coelhos , Animais , Complemento C6/análise , Complemento C6/genética , Complemento C8/análise , Complemento C8/genética , Feminino , Masculino , Linhagem , Coelhos/genética
20.
Hum Hered ; 35(1): 30-3, 1985.
Artigo em Inglês | MEDLINE | ID: mdl-3882555

RESUMO

Agarose gel isoelectric focusing was used to investigate the genetic polymorphism of the sixth component of complement (C6) in Japanese. C6 patterns were visualized by the immunofixation procedure. The allele frequencies calculated from 135 individuals were as follows: C6*A = 0.467, C6*B = 0.481, C6*B2 = 0.037, and C6*B3 = 0.015. It is suggested that C6*B3 is the fourth common allele characterizing the Japanese population.


Assuntos
Complemento C6/genética , Adulto , Alelos , Eletroforese das Proteínas Sanguíneas , Criança , Complemento C6/análise , Eletroforese em Gel de Ágar , Feminino , Frequência do Gene , Técnica de Placa Hemolítica , Humanos , Focalização Isoelétrica , Japão , Masculino , Polimorfismo Genético , Gravidez , Gêmeos Monozigóticos
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